Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP10_F_D11
(891 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 188 9e-49
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 177 1e-45
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 103 5e-23
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 93 5e-20
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 28 2.1
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.3
SPAC4G9.04c |||cleavage and polyadenylation specificity factor |... 26 6.3
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.3
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 188 bits (458), Expect = 9e-49
Identities = 80/115 (69%), Positives = 97/115 (84%)
Frame = +3
Query: 222 CLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYR 401
CLEHGIQP+G M + D F+TFFSETG GK+VPR+++VDLEP V+D+VRTG YR
Sbjct: 25 CLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYR 84
Query: 402 QLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFH 566
LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH
Sbjct: 85 DLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFH 139
Score = 78.2 bits (184), Expect = 1e-15
Identities = 35/43 (81%), Positives = 39/43 (90%)
Frame = +3
Query: 762 VDNEAIYDICRRNLDIERPTYTNLNRLIXQIVSSITXSLRFDG 890
VDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSIT SLRF+G
Sbjct: 204 VDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEG 246
Score = 56.4 bits (130), Expect = 5e-09
Identities = 27/53 (50%), Positives = 36/53 (67%)
Frame = +2
Query: 602 LIDGASLRLTTARSLNWSSPSTPRPQVSTAVVEPYNSILTTHTTLEHSDCAFM 760
L++ ++ T L +S P PQVST+VVEPYNS+LTTH TL+ +DC FM
Sbjct: 153 LLERLAMEYTKKSKLQFSV--YPAPQVSTSVVEPYNSVLTTHATLDLADCTFM 203
Score = 52.0 bits (119), Expect = 1e-07
Identities = 21/24 (87%), Positives = 22/24 (91%)
Frame = +2
Query: 149 MRECISVHVGQAGVQIGNACWELY 220
MRE IS+HVGQAG QIGNACWELY
Sbjct: 1 MREIISIHVGQAGTQIGNACWELY 24
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 177 bits (432), Expect = 1e-45
Identities = 79/120 (65%), Positives = 98/120 (81%), Gaps = 5/120 (4%)
Frame = +3
Query: 222 CLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVR 386
CLEHGI PDG PT+ K +D F TFFSETG GK VPR+++VDLEP V+D+VR
Sbjct: 25 CLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVR 83
Query: 387 TGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFH 566
TG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH
Sbjct: 84 TGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFH 143
Score = 79.4 bits (187), Expect = 6e-16
Identities = 37/43 (86%), Positives = 38/43 (88%)
Frame = +3
Query: 762 VDNEAIYDICRRNLDIERPTYTNLNRLIXQIVSSITXSLRFDG 890
VDNEA YDICRRNLDIERPTY NLNRLI Q+VSSIT SLRF G
Sbjct: 208 VDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAG 250
Score = 58.8 bits (136), Expect = 1e-09
Identities = 24/31 (77%), Positives = 28/31 (90%)
Frame = +2
Query: 668 PRPQVSTAVVEPYNSILTTHTTLEHSDCAFM 760
P PQVST+VVEPYNS+LTTH TL++SDC FM
Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSDCTFM 207
Score = 54.4 bits (125), Expect = 2e-08
Identities = 27/44 (61%), Positives = 31/44 (70%), Gaps = 1/44 (2%)
Frame = +2
Query: 149 MRECISVHVGQAGVQIGNACWELY-LPGARHPA*WPDAHRQDHR 277
MRE ISVHVGQAGVQIGNACWELY L P +P + + H+
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHK 44
Score = 32.3 bits (70), Expect = 0.095
Identities = 11/16 (68%), Positives = 15/16 (93%)
Frame = +1
Query: 628 DYGKKSKLEFAIYPAP 675
+YGKKS L+F++YPAP
Sbjct: 164 EYGKKSNLQFSVYPAP 179
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 103 bits (246), Expect = 5e-23
Identities = 49/113 (43%), Positives = 68/113 (60%)
Frame = +3
Query: 228 EHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQL 407
EHG+ G T + N +F+E GK+VPRAV VDLEP +D V++G + L
Sbjct: 27 EHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNL 84
Query: 408 FHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFH 566
F P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C LQGF + H
Sbjct: 85 FRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTH 137
Score = 41.9 bits (94), Expect = 1e-04
Identities = 17/43 (39%), Positives = 27/43 (62%)
Frame = +3
Query: 762 VDNEAIYDICRRNLDIERPTYTNLNRLIXQIVSSITXSLRFDG 890
+DNEA+ I L I+ P+Y +LN L+ +++ +T S RF G
Sbjct: 202 IDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPG 244
Score = 35.1 bits (77), Expect = 0.014
Identities = 15/30 (50%), Positives = 20/30 (66%)
Frame = +2
Query: 668 PRPQVSTAVVEPYNSILTTHTTLEHSDCAF 757
P P+ S VVEPYN+ L+ H +E+SD F
Sbjct: 171 PAPKSSDTVVEPYNATLSMHQLVENSDETF 200
Score = 27.5 bits (58), Expect = 2.7
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +2
Query: 149 MRECISVHVGQAGVQIGNACW 211
MRE + + GQ G Q+G A W
Sbjct: 1 MREIVHIQAGQCGNQVGAAFW 21
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 93.1 bits (221), Expect = 5e-20
Identities = 59/225 (26%), Positives = 100/225 (44%), Gaps = 2/225 (0%)
Frame = +3
Query: 222 CLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYR 401
CLEHGI PDG + + T G D + FF ++ +++PRA+ +DLEP VV+ + + TY
Sbjct: 26 CLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYG 83
Query: 402 QLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXX 575
L++PE ++ K A NN+A G Y+ + I + ++D I + AD L+GF + H
Sbjct: 84 SLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIA 142
Query: 576 XXXXXXXXXXXXXXXXXXXXQEV*TGVRHLPRALRFXXXXXXXXXXXXXXXXXXXXXXXX 755
+++ P +
Sbjct: 143 GGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSV 202
Query: 756 XWVDNEAIYDICRRNLDIERPTYTNLNRLIXQIVSSITXSLRFDG 890
+DN A+ I L + PT+ N+L+ ++S+ T +LR+ G
Sbjct: 203 VVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPG 247
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 27.9 bits (59), Expect = 2.1
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = -2
Query: 884 ESQRXSDXGDNLSDETIQVGVGWALNVEITAADVIDG 774
+ Q+ SD ++ +D T VG+G AL I + D +DG
Sbjct: 182 DKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 26.2 bits (55), Expect = 6.3
Identities = 16/38 (42%), Positives = 20/38 (52%)
Frame = +1
Query: 220 LAWSTASSLMARCPQTRPSGVETILSTLSSARPELAST 333
L ST SSL + ++PS T ST SSA P S+
Sbjct: 170 LTSSTFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPAC4G9.04c |||cleavage and polyadenylation specificity factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 638
Score = 26.2 bits (55), Expect = 6.3
Identities = 12/32 (37%), Positives = 22/32 (68%)
Frame = +2
Query: 617 SLRLTTARSLNWSSPSTPRPQVSTAVVEPYNS 712
++ LT L+ SS +TPRP++S+ + E Y++
Sbjct: 390 AVSLTADIDLSKSSLATPRPKLSSLLYENYSN 421
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.3
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 248 WPDAHRQDHRGWRRFFQHFLQR 313
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,543,621
Number of Sequences: 5004
Number of extensions: 74439
Number of successful extensions: 236
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 206
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 233
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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