Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP10_F_D01
(874 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase |Schi... 99 4e-22
SPAC186.07c |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 60 3e-10
SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosac... 59 9e-10
SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 56 5e-09
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 33 0.070
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 33 0.070
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 30 0.49
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 28 1.5
SPAC23G3.12c |||serine protease |Schizosaccharomyces pombe|chr 1... 28 2.0
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 28 2.0
SPAC23A1.15c |sec20||SNARE Sec20|Schizosaccharomyces pombe|chr 1... 27 2.6
SPBC947.15c |||NADH dehydrogenase|Schizosaccharomyces pombe|chr ... 27 2.6
SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces pombe... 27 3.5
SPBC725.11c |php2||CCAAT-binding factor complex subunit Php2 |Sc... 27 3.5
SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc... 27 4.6
SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|ch... 27 4.6
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 27 4.6
SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces p... 26 6.1
>SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 466
Score = 99 bits (238), Expect = 4e-22
Identities = 55/172 (31%), Positives = 95/172 (55%), Gaps = 1/172 (0%)
Frame = +3
Query: 132 DIKSVLIVDGVGAKCAELLNAYGIATT-TKAKISKEELLMEIPNHDALVVRSATQVTKEV 308
DIK +L+++ V L G K +S+++L+ +I A+ +RS T++T+ V
Sbjct: 55 DIK-ILLLENVNQSALSNLKDEGYQVEFLKTSMSEDDLVEKIKGVHAIGIRSKTRLTRRV 113
Query: 309 LDAGVKLKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVP 488
L+A L V+G G + +D+D A ++G+ V N+P AN+ S EL ++ LAR V
Sbjct: 114 LEAADSLIVIGCFCIGTNQVDLDFAAERGIAVFNSPYANSRSVAELVIGYIISLARQVGD 173
Query: 489 ASTALKAGRWDRALYTGSELAGKTLAILGLGRVGREVATRMYAFGMNIIGFD 644
S L G W++ E+ GKTL I+G G +G +++ A G++++ +D
Sbjct: 174 RSLELHRGEWNKVSSGCWEIRGKTLGIIGYGHIGSQLSVLAEAMGLHVVYYD 225
>SPAC186.07c |||hydroxyacid dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 332
Score = 60.5 bits (140), Expect = 3e-10
Identities = 46/171 (26%), Positives = 77/171 (45%), Gaps = 1/171 (0%)
Frame = +3
Query: 330 KVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASTALKA 509
K++ AG +N+D+ +A G+ V+ P + + E T L+L L R + A ++
Sbjct: 70 KLIALRCAGFNNVDLKAAADNGITVVRVPAYSPYAVAEYTIGLLLSLNRKIHRAYVRVRE 129
Query: 510 GRWDRALYTGSELAGKTLAILGLGRVGREVATRM-YAFGMNIIGFDPFVSADQCAQFHCT 686
++ G +L GKT+ +LG GR+G VA + FG ++ D + +F
Sbjct: 130 DDFNLNGLLGHDLHGKTIGLLGTGRIGGLVAKCLKLGFGCEVLAHD-IKPNKELEKFGIQ 188
Query: 687 KMELEDIWPLADYITLAHSGSLSLLETLSMPMS*XQCXKGVXIITWVEAGL 839
+E +++ AD++ L H E L KGV II GL
Sbjct: 189 FVEQQEVLAKADFLCL-HCPLTPDTEHLVDEKLLASMKKGVKIINTSRGGL 238
>SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 340
Score = 58.8 bits (136), Expect = 9e-10
Identities = 32/104 (30%), Positives = 50/104 (48%)
Frame = +3
Query: 324 KLKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASTAL 503
KL V G AG +N+DVD A + GV V N P + L + R A +L
Sbjct: 85 KLFVTG--AAGYNNVDVDWATRNGVYVANTPNGPTEGTANMNLMLFMCTLRGAREAEQSL 142
Query: 504 KAGRWDRALYTGSELAGKTLAILGLGRVGREVATRMYAFGMNII 635
+ G+W + L + GK + I+G+G +G+ A ++ G I+
Sbjct: 143 RLGKWRQNLSLTDDPYGKRVGIIGMGAIGKSFAQKILPLGCEIV 186
>SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 334
Score = 56.4 bits (130), Expect = 5e-09
Identities = 35/113 (30%), Positives = 54/113 (47%), Gaps = 1/113 (0%)
Frame = +3
Query: 300 KEVLD-AGVKLKVVGRAGAGVDNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLAR 476
KE++D +K + GAG + +DV + +G+ V + P A + ++ LML R
Sbjct: 71 KEIIDNLPPSVKFICHLGAGYETVDVAACTARGIQVSHVPKAVDDATADVGIFLMLGALR 130
Query: 477 HVVPASTALKAGRWDRALYTGSELAGKTLAILGLGRVGREVATRMYAFGMNII 635
L W+ + GKTL ILGLG +G+ +A R AF M I+
Sbjct: 131 GFNQGIFELHKNNWNANCKPSHDPEGKTLGILGLGGIGKTMAKRARAFDMKIV 183
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 32.7 bits (71), Expect = 0.070
Identities = 33/98 (33%), Positives = 44/98 (44%), Gaps = 1/98 (1%)
Frame = -1
Query: 412 ALITP-TPFLPAESTSMLSTPAPARPTTFSFTPASNTSLVT*VAERTTRASWLGISIRSS 236
+L TP TP +P STS S P P PT+ S T ++ L T TT S + + SS
Sbjct: 129 SLSTPITPTVPPTSTSSTSIPIP--PTSTSSTDTNSNPLPTTSTSCTTSTS-IPPTGGSS 185
Query: 235 SLEXXXXXXXXXXXXLRSSAHLAPTPSTIKTDLMSTTI 122
SL +S + PT ST TD S+ +
Sbjct: 186 SLSTPITPTVPPTSTSSTSIPIPPT-STSSTDTNSSPL 222
Score = 31.1 bits (67), Expect = 0.21
Identities = 33/98 (33%), Positives = 44/98 (44%), Gaps = 1/98 (1%)
Frame = -1
Query: 412 ALITP-TPFLPAESTSMLSTPAPARPTTFSFTPASNTSLVT*VAERTTRASWLGISIRSS 236
+L TP TP +P STS S P P PT+ S T +++ L T TT S + SS
Sbjct: 186 SLSTPITPTVPPTSTSSTSIPIP--PTSTSSTDTNSSPLPTTSTSCTTSTSI--PTGGSS 241
Query: 235 SLEXXXXXXXXXXXXLRSSAHLAPTPSTIKTDLMSTTI 122
SL +S + PT ST TD S+ +
Sbjct: 242 SLSTPITPTVPPTSTSSTSIPIPPT-STSSTDTNSSPL 278
Score = 30.3 bits (65), Expect = 0.37
Identities = 30/115 (26%), Positives = 48/115 (41%), Gaps = 2/115 (1%)
Frame = -1
Query: 601 ATSRPTLPRPRMARVLPASSLPVYRARSHLPAFSAVEAGTTWRARTNINVQVSSHALSAL 422
+TS PT ++ + + P + + +P + T + S +++
Sbjct: 232 STSIPTGGSSSLSTPITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTSTSCTTSTSI 291
Query: 421 AP-GALITP-TPFLPAESTSMLSTPAPARPTTFSFTPASNTSLVT*VAERTTRAS 263
P G TP TP +P STS STP P P + S T S++ L + TT S
Sbjct: 292 PPTGNSTTPVTPTVPPTSTSSTSTPPP--PASTSSTGTSSSPLPSTSTSCTTSTS 344
Score = 29.9 bits (64), Expect = 0.49
Identities = 21/52 (40%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = -1
Query: 415 GALITP-TPFLPAESTSMLSTPAPARPTTFSFTPASNTSLVT*VAERTTRAS 263
G TP TP +P STS STP P P + S T S++ L++ TT S
Sbjct: 349 GNSTTPVTPTVPPTSTSSTSTPPP--PASTSSTGTSSSPLLSTSTSCTTSTS 398
Score = 26.2 bits (55), Expect = 6.1
Identities = 19/68 (27%), Positives = 27/68 (39%)
Frame = -1
Query: 466 TNINVQVSSHALSALAPGALITPTPFLPAESTSMLSTPAPARPTTFSFTPASNTSLVT*V 287
T N S+ L P +T TP T+ S P + P T S S+++ VT
Sbjct: 528 TTTNCTTSTSVLYTSTP---VTSTPLATTNCTTSTSVPYTSTPVTSSNYTISSSTPVTST 584
Query: 286 AERTTRAS 263
TT +
Sbjct: 585 PVTTTNCT 592
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 32.7 bits (71), Expect = 0.070
Identities = 26/84 (30%), Positives = 39/84 (46%), Gaps = 1/84 (1%)
Frame = -1
Query: 550 ASSLPVYRARSHLPAFSAVEAGTTWRARTNINVQVSS-HALSALAPGALITPTPFLPAES 374
+SSLP + S P+ S++ T+ + +V SS H + P L P+ F+ A+
Sbjct: 116 SSSLPSIPSSSSTPSISSIPHTTSSVSNDIPSVLGSSDHPIDLDNPEHLTPPSSFITAKQ 175
Query: 373 TSMLSTPAPARPTTFSFTPASNTS 302
S L TP P P + S P S
Sbjct: 176 LSRLPTPLP--PPSSSSLPTGTIS 197
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 29.9 bits (64), Expect = 0.49
Identities = 17/48 (35%), Positives = 25/48 (52%)
Frame = +1
Query: 49 SVAARRSEEAHTSIKLNNDSTTKPRWSWTLSRF*SSTGLAPSVPNSST 192
SVAA R+ A ++ NND T P+ + +S F S G + + P T
Sbjct: 415 SVAALRARFAKANVSENNDPPTFPKTAAKISSFNSKAGTSFAKPRPFT 462
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 28.3 bits (60), Expect = 1.5
Identities = 24/104 (23%), Positives = 52/104 (50%), Gaps = 3/104 (2%)
Frame = -1
Query: 610 ILVATSRPTLPRPRMARVLPASSLPVYRARSHLPAFSAVEAGTTWRARTNINVQVS---S 440
I+V++ T+ +P RV + + + P S + + + +++ Q S S
Sbjct: 427 IIVSSYVSTVTQPASTRVQTTTVSSISTSVKQ-PTASVASSSVSVPSSSSVQPQSSTPIS 485
Query: 439 HALSALAPGALITPTPFLPAESTSMLSTPAPARPTTFSFTPASN 308
+ SA +P + ++ + + +E +S L + + A P+T S TP+S+
Sbjct: 486 SSSSASSPQSTLSTSSEVVSEVSSTLLSGSSAIPSTSSSTPSSS 529
>SPAC23G3.12c |||serine protease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 996
Score = 27.9 bits (59), Expect = 2.0
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = -3
Query: 668 ALISRHERVESDDVHAEGVHPSGHLAADPAKAK 570
A+ HE VE V+ + VH G L DP K +
Sbjct: 112 AVFDNHEEVEVFPVYRDPVHDFGFLRFDPKKIR 144
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 27.9 bits (59), Expect = 2.0
Identities = 24/85 (28%), Positives = 40/85 (47%)
Frame = -1
Query: 379 ESTSMLSTPAPARPTTFSFTPASNTSLVT*VAERTTRASWLGISIRSSSLEXXXXXXXXX 200
ES+S+ +T +P +TF T +S +S+ T + ++ S +S+ SSS
Sbjct: 238 ESSSLTNTVSPTE-STFYETKSSTSSVPTQTIDSSSFTSSTPVSLTSSSTS--------S 288
Query: 199 XXXLRSSAHLAPTPSTIKTDLMSTT 125
+ S + TPSTI T + T
Sbjct: 289 SGSSQDSTTIDSTPSTIATSTLQPT 313
>SPAC23A1.15c |sec20||SNARE Sec20|Schizosaccharomyces pombe|chr
1|||Manual
Length = 226
Score = 27.5 bits (58), Expect = 2.6
Identities = 13/27 (48%), Positives = 19/27 (70%)
Frame = +1
Query: 22 HYREFLKIWSVAARRSEEAHTSIKLNN 102
H+REF KIW A R E ++SI+L++
Sbjct: 27 HFREFRKIWETA--RVELEYSSIQLDS 51
>SPBC947.15c |||NADH dehydrogenase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 551
Score = 27.5 bits (58), Expect = 2.6
Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 1/70 (1%)
Frame = -1
Query: 529 RARSHLPAFSAVEAGTTWRARTNI-NVQVSSHALSALAPGALITPTPFLPAESTSMLSTP 353
+ +S +P + V G+ W A I N+ S + ++ ++P TP LP+ + L P
Sbjct: 83 KGKSGVPKKNIVVLGSGWGAVAAIKNLDPSLYNITLVSPRDHFLFTPMLPSCTVGTLRLP 142
Query: 352 APARPTTFSF 323
+ P F
Sbjct: 143 SITEPIVALF 152
>SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 27.1 bits (57), Expect = 3.5
Identities = 19/54 (35%), Positives = 28/54 (51%), Gaps = 4/54 (7%)
Frame = +3
Query: 546 LAGKTLAILGLGRVGREVATRMYAFGMNII--GFDPFVSADQCAQ--FHCTKME 695
+AGK + G G VG+ +T + + G +I DP ++A Q A F T ME
Sbjct: 212 IAGKVAVVAGFGDVGKGCSTSLRSQGARVIVTEVDP-INALQAAMDGFEVTTME 264
>SPBC725.11c |php2||CCAAT-binding factor complex subunit Php2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 334
Score = 27.1 bits (57), Expect = 3.5
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -1
Query: 385 PAESTSMLSTPAPARPTTFSFTPASNTS 302
P+ ++ +PAP T S +PA+NTS
Sbjct: 125 PSSISNSSESPAPINSATASMSPANNTS 152
>SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase
Shk1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 658
Score = 26.6 bits (56), Expect = 4.6
Identities = 15/56 (26%), Positives = 26/56 (46%)
Frame = -1
Query: 436 ALSALAPGALITPTPFLPAESTSMLSTPAPARPTTFSFTPASNTSLVT*VAERTTR 269
++SAL+ + + PT + S S PAP P + S +P ++ V +R
Sbjct: 229 SVSALSSSSHLQPTSATSSSSRLYPSRPAPTPPASSSSSPLLSSQTVKTTTSNASR 284
>SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 867
Score = 26.6 bits (56), Expect = 4.6
Identities = 14/42 (33%), Positives = 19/42 (45%)
Frame = -1
Query: 535 VYRARSHLPAFSAVEAGTTWRARTNINVQVSSHALSALAPGA 410
+ R R HL + A E T WR + + + LS L P A
Sbjct: 334 IRRLRGHLREYVAWERNTIWREMMAMERRTQAARLSGLKPVA 375
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 26.6 bits (56), Expect = 4.6
Identities = 16/62 (25%), Positives = 28/62 (45%)
Frame = +3
Query: 231 KEELLMEIPNHDALVVRSATQVTKEVLDAGVKLKVVGRAGAGVDNIDVDSAGKKGVGVIN 410
KEE +I + +TQ+ + +K + + A +D+I DS G G +N
Sbjct: 660 KEEAQKQISQSLIADQQLSTQIGSVGMGKKQAVKELRKLQAKIDSIIADSMSPMGTGAVN 719
Query: 411 AP 416
+P
Sbjct: 720 SP 721
>SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 584
Score = 26.2 bits (55), Expect = 6.1
Identities = 22/70 (31%), Positives = 31/70 (44%), Gaps = 4/70 (5%)
Frame = -3
Query: 587 DPAKAKDGEGLASELAASVQSPVPP-ASLQRSGSWDHVASQNQHQRAGQLARAQRI---G 420
DP+K+KDG L S V P PP + + +H + + R +L R Q G
Sbjct: 293 DPSKSKDGLNLGS--LTDVVPPQPPQPEHELAALQEHNEAHDAAMRQYELERQQNAMLPG 350
Query: 419 ARSVDHSDAL 390
S+D AL
Sbjct: 351 LASIDGLQAL 360
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,291,025
Number of Sequences: 5004
Number of extensions: 65030
Number of successful extensions: 237
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 210
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 235
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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