Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP09_F_P20
(866 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa... 87 2e-18
SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz... 60 3e-10
SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac... 38 0.002
SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyce... 29 0.65
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 27 3.5
SPAC17C9.12 |||MSP domain|Schizosaccharomyces pombe|chr 1|||Manual 27 3.5
SPBC106.07c |||N alpha-acetyltransferase Nat2 |Schizosaccharomyc... 26 8.0
>SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 210
Score = 87.4 bits (207), Expect = 2e-18
Identities = 49/169 (28%), Positives = 88/169 (52%), Gaps = 4/169 (2%)
Frame = +1
Query: 169 LSKSVXLTFXLPDSAEACFY-QTMDKDTAASLEYQVISGGQYDVSVKIEAPNRQIIYQQE 345
L+ ++ LTF L + + C+Y + Y V SGG +DV I+AP+++ + +
Sbjct: 17 LASAIELTFKLENQEKQCYYLDSFHTGEKTHFTYAVQSGGSFDVDYMIKAPSQKTVALGK 76
Query: 346 KMQYDSHQFTALASGDYKVCFSNEFSTFSHKLVYMELNVGPEQPLPGV-GDHATVLTQ-- 516
K + FT G+Y+ CF N STF+ K+V ME+ + E LP + D A +
Sbjct: 77 KRRQADVFFTLEEKGEYEFCFDNHMSTFTDKIVTMEITMENELSLPALTRDEAKDYKKDS 136
Query: 517 LETSAEEIHSALNRIIDHQTHHRLREAQGRKRAEDLNEKVFWWSMGETL 663
++++ EI +AL+ I Q + + RE + + ++FW+S+ E++
Sbjct: 137 MQSTVLEISTALSEIDRVQNYFKTREHRNYSTVKSTQARIFWFSLAESI 185
>SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 199
Score = 60.5 bits (140), Expect = 3e-10
Identities = 38/148 (25%), Positives = 64/148 (43%)
Frame = +1
Query: 220 CFYQTMDKDTAASLEYQVISGGQYDVSVKIEAPNRQIIYQQEKMQYDSHQFTALASGDYK 399
CFY+ + + S+ YQ GG VS+ I P QI++Q+ + FT G Y
Sbjct: 33 CFYENLRNNDQMSVTYQTNVGGDQLVSMSIYNPAGQIMHQEVPNSMAQYSFTVKNPGKYM 92
Query: 400 VCFSNEFSTFSHKLVYMELNVGPEQPLPGVGDHATVLTQLETSAEEIHSALNRIIDHQTH 579
CF N+ K V ++ + + L ++H ++++ Q +
Sbjct: 93 YCFYNDALDGESKEVLFNVH----GVIYISDEDLDANNPLLGKVRQLHDTISKVKHEQEY 148
Query: 580 HRLREAQGRKRAEDLNEKVFWWSMGETL 663
RE R AE N++V WWS+ +T+
Sbjct: 149 FVARERIHRNTAESTNDRVKWWSILQTV 176
>SPBC16E9.09c |||COPII vesicle coat component
Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 215
Score = 37.5 bits (83), Expect = 0.002
Identities = 21/113 (18%), Positives = 49/113 (43%), Gaps = 2/113 (1%)
Frame = +1
Query: 319 NRQIIYQQEKMQYDSHQFTALASGDYKVCF--SNEFSTFSHKLVYMELNVGPEQPLPGVG 492
N +IY+ + ++ +FT G +++C+ +++ + FS + +++ + +
Sbjct: 76 NNHLIYKNDLRSNEAVRFTTTNEGTHRICYLSTSDGAWFSSTKTRLHVDLASDDKYNLIA 135
Query: 493 DHATVLTQLETSAEEIHSALNRIIDHQTHHRLREAQGRKRAEDLNEKVFWWSM 651
L +++ L I+ Q R RE R +E N +V W++
Sbjct: 136 SSEDEAKDLTDRVAALNTRLESILGEQMLQRSREMTFRDTSESANSRVVRWTI 188
>SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 818
Score = 29.5 bits (63), Expect = 0.65
Identities = 28/100 (28%), Positives = 43/100 (43%), Gaps = 7/100 (7%)
Frame = -1
Query: 509 RTVA*SPTPGRGCSGPTFSSIYTSLCENVLNSLLKHTL*SPDARAVNWCESYCIFSC*YI 330
R+ SPT PT + + E V N+L K+ S R + Y S Y+
Sbjct: 460 RSTTTSPTSAVASPAPTENEDSNAAIERVANTLRKNPTISRRTRRAGTMDRYATASSDYM 519
Query: 329 ICLLGA--SIFTLTSYCPPE--ITW---YSNEAAVSLSIV 231
LG+ ++ TL+ P+ TW + N + +S SIV
Sbjct: 520 ESNLGSLPNLSTLSLQSGPDSTATWHPEFPNSSGLSASIV 559
>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 27.1 bits (57), Expect = 3.5
Identities = 22/81 (27%), Positives = 37/81 (45%), Gaps = 1/81 (1%)
Frame = -2
Query: 268 GTPMKQPYPCPLFDKNMLRHYQEVQTLTRHFSKEHHEXXEXRXXRKAEPETXEXE*KNXE 89
G M Q + LF++ +L+ Y+E + + + E E RK E E + K +
Sbjct: 540 GRRMFQIFAARLFEQRVLQAYRE--KVAQQRQAKLLEEIEEENKRKQERELKKIREKEKK 597
Query: 88 KD-KKXLRXRGLQXRQKXXRE 29
+D KK L+ + RQ+ E
Sbjct: 598 RDKKKQLKLAKEEERQRREAE 618
>SPAC17C9.12 |||MSP domain|Schizosaccharomyces pombe|chr 1|||Manual
Length = 319
Score = 27.1 bits (57), Expect = 3.5
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -1
Query: 680 QHXQXPKVSPIDHQNTFSFRSSALLRP*ASL 588
QH Q PK S + HQ S+A+ P A++
Sbjct: 190 QHTQLPKTSAVSHQKPHEAPSTAVKAPTATV 220
>SPBC106.07c |||N alpha-acetyltransferase Nat2 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 167
Score = 25.8 bits (54), Expect = 8.0
Identities = 9/30 (30%), Positives = 16/30 (53%)
Frame = +1
Query: 607 KRAEDLNEKVFWWSMGETLGYCXCXIXTSY 696
+R ++L +K WWS+G +G S+
Sbjct: 52 QRVKELTKKYGWWSLGVYIGISVLDFSASF 81
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,511,934
Number of Sequences: 5004
Number of extensions: 49227
Number of successful extensions: 127
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 125
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 432473040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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