Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP09_F_M21
         (922 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch...   132   7e-32
SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr...   116   6e-27
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc...    33   0.057
SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosacc...    26   0.28 
SPBC1347.10 |cdc23|mcm10|MCM-associated protein Mcm10|Schizosacc...    27   3.7  

>SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 778

 Score =  132 bits (319), Expect = 7e-32
 Identities = 61/111 (54%), Positives = 77/111 (69%)
 Frame = +3

Query: 63  LKSTGRHDIAAXANSYKHLLTPDNKAPYDQLIEIDLSTLEPHVNGPFTPDLANPISXLGE 242
           L++T R  IA  A  +  ++  D  A YDQ+IEIDL+TLEPH+NGPFTPDLA PIS   E
Sbjct: 303 LRATNRSAIADYAEEFAPIIAADENAHYDQIIEIDLNTLEPHLNGPFTPDLATPISKFKE 362

Query: 243 AAKXNXWPVDIRVGLIGSCTXSSYEDMGXCASXVKEAISHGLXSKIPFXVT 395
           A K N WP +++VGLIGSCT SSYEDM   AS  ++AI  G+ +K  F +T
Sbjct: 363 AVKKNDWPQELKVGLIGSCTNSSYEDMSRAASICQQAIDKGIKTKSLFTIT 413



 Score = 31.9 bits (69), Expect = 0.13
 Identities = 15/31 (48%), Positives = 18/31 (58%)
 Frame = +1

Query: 397 PASXXVRATIXRDGXANTLTDFGRPVLXNXC 489
           P S  VRAT+ RDG  +T+   G  VL N C
Sbjct: 414 PGSEQVRATLTRDGQLDTMRKAGGIVLANAC 444


>SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 905

 Score =  116 bits (278), Expect = 6e-27
 Identities = 54/104 (51%), Positives = 69/104 (66%)
 Frame = +3

Query: 84  DIAAXANSYKHLLTPDNKAPYDQLIEIDLSTLEPHVNGPFTPDLANPISXLGEAAKXNXW 263
           D A+  +S  + L  D  A YDQ+I+I+LS L P +NGPFTPDL+ P+S  GEA + N W
Sbjct: 317 DAASEVHSEYNYLAADTGAKYDQIIDINLSELTPSLNGPFTPDLSTPVSKFGEAIEKNKW 376

Query: 264 PVDIRVGLIGSCTXSSYEDMGXCASXVKEAISHGLXSKIPFXVT 395
           P  +  GLIGSCT SSY+DM      V++AIS GL  K+PF VT
Sbjct: 377 PKKLSAGLIGSCTNSSYQDMTCVVDVVEQAISAGLKPKVPFLVT 420



 Score = 33.1 bits (72), Expect = 0.057
 Identities = 15/31 (48%), Positives = 17/31 (54%)
 Frame = +1

Query: 397 PASXXVRATIXRDGXANTLTDFGRPVLXNXC 489
           P S  +RATI RDG    L + G  VL N C
Sbjct: 421 PGSEQIRATIERDGITERLEEAGATVLANAC 451


>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 721

 Score = 33.1 bits (72), Expect = 0.057
 Identities = 23/73 (31%), Positives = 32/73 (43%)
 Frame = +3

Query: 129 DNKAPYDQLIEIDLSTLEPHVNGPFTPDLANPISXLGEAAKXNXWPVDIRVGLIGSCTXS 308
           D  A Y + + +DLSTL P V+GP      N +     AA      + I+   + SCT  
Sbjct: 327 DEGAFYAKHLILDLSTLSPAVSGP------NSVKVYNSAATLEKKDILIKKAYLVSCTNG 380

Query: 309 SYEDMGXCASXVK 347
              D+   A  VK
Sbjct: 381 RLSDIHDAAETVK 393


>SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 758

 Score = 25.8 bits (54), Expect(2) = 0.28
 Identities = 14/29 (48%), Positives = 19/29 (65%)
 Frame = +3

Query: 261 WPVDIRVGLIGSCTXSSYEDMGXCASXVK 347
           +P+D +V  IGSCT S  ED+   A+ VK
Sbjct: 349 YPID-KV-FIGSCTNSRIEDLRLAAAVVK 375



 Score = 23.4 bits (48), Expect(2) = 0.28
 Identities = 14/44 (31%), Positives = 21/44 (47%)
 Frame = +3

Query: 99  ANSYKHLLTPDNKAPYDQLIEIDLSTLEPHVNGPFTPDLANPIS 230
           A +Y   L  D  A YD  +EI+ + + P V    +P    PI+
Sbjct: 270 AVAYWKTLRSDENAKYDIEVEINAADVLPTVTWGTSPQDVIPIN 313


>SPBC1347.10 |cdc23|mcm10|MCM-associated protein
           Mcm10|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 593

 Score = 27.1 bits (57), Expect = 3.7
 Identities = 16/58 (27%), Positives = 25/58 (43%)
 Frame = +3

Query: 63  LKSTGRHDIAAXANSYKHLLTPDNKAPYDQLIEIDLSTLEPHVNGPFTPDLANPISXL 236
           +++ G   +    +  K+L    N     +  EID ST +P V   F   + NP S L
Sbjct: 438 MRTLGHQYLNLQGSGVKNLHDKGNPTALSKDSEIDSSTKKPSVLASFNASIMNPKSSL 495


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,577,758
Number of Sequences: 5004
Number of extensions: 18832
Number of successful extensions: 54
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 51
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 54
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 468512460
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)