BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP09_F_M12
(899 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 36 0.008
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.29
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 0.90
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 2.7
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 3.6
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 26 8.4
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 35.9 bits (79), Expect = 0.008
Identities = 21/65 (32%), Positives = 23/65 (35%), Gaps = 6/65 (9%)
Frame = +2
Query: 584 PPXXXPXXXFFKKXP-PPPXGXFFFFXXXKKKXXXPPPPPXKKKKXXPPPXG-----XXX 745
P P K P PPP K PPPPP ++ PP G
Sbjct: 277 PVSMNPAINSTSKPPLPPPSSRVSAAALAANKKRPPPPPPPSRRNRGKPPIGNGSSNSSL 336
Query: 746 PPPPP 760
PPPPP
Sbjct: 337 PPPPP 341
Score = 34.3 bits (75), Expect = 0.024
Identities = 25/105 (23%), Positives = 28/105 (26%), Gaps = 6/105 (5%)
Frame = +3
Query: 462 PXKKKPXXXGKKKXPPXXFFXIPPGGXXGGXKNFFXXKXXPPPXXPPXXXFLKKXPPPPX 641
P + P P P G N P P K PPP
Sbjct: 237 PSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPPPS 296
Query: 642 GXFFFXXXXKKKXXXPPPPXXKKKKXXPPPXG------XXPPPPP 758
K PPPP ++ PP G PPPPP
Sbjct: 297 SRVSAAALAANKKRPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPP 341
Score = 32.7 bits (71), Expect = 0.073
Identities = 21/66 (31%), Positives = 23/66 (34%), Gaps = 3/66 (4%)
Frame = +2
Query: 572 KXXPPPXXXPXXXFFKKXPPPPXGXFFFFXXXKKKXXXPPPPPXKKKKXXP---PPXGXX 742
K PPP P + PP G PPPPP + PP G
Sbjct: 308 KKRPPPPPPPSR---RNRGKPPIGN-----GSSNSSLPPPPPPPRSNAAGSIPLPPQGRS 359
Query: 743 XPPPPP 760
PPPPP
Sbjct: 360 APPPPP 365
Score = 27.1 bits (57), Expect = 3.6
Identities = 16/68 (23%), Positives = 19/68 (27%), Gaps = 3/68 (4%)
Frame = +2
Query: 581 PPPXXXPXXXFFKKXP---PPPXGXFFFFXXXKKKXXXPPPPPXKKKKXXPPPXGXXXPP 751
PPP P P P P P PPP + PP
Sbjct: 253 PPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPPPSSRVSAAALAANKKRPP 312
Query: 752 PPPXXKKK 775
PPP ++
Sbjct: 313 PPPPPSRR 320
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 30.7 bits (66), Expect = 0.29
Identities = 18/50 (36%), Positives = 18/50 (36%), Gaps = 3/50 (6%)
Frame = +2
Query: 620 KXPPPPXGXFFFFXXXKKKXXXPPPPPXKK-KKXXPPPXGX--XXPPPPP 760
K PPPP PPP P PPP G PPPPP
Sbjct: 730 KSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP 779
Score = 29.5 bits (63), Expect = 0.68
Identities = 13/25 (52%), Positives = 13/25 (52%)
Frame = +2
Query: 686 PPPPPXKKKKXXPPPXGXXXPPPPP 760
PPPPP PPP PPPPP
Sbjct: 763 PPPPPPGVAGAGPPP----PPPPPP 783
Score = 25.8 bits (54), Expect = 8.4
Identities = 17/55 (30%), Positives = 17/55 (30%), Gaps = 8/55 (14%)
Frame = +3
Query: 615 LKKXPPPPXGXFFFXXXXKKKXXXPPPPXXKKKKXXPPPXGX--------XPPPP 755
LK PPPP PP P PPP G PPPP
Sbjct: 729 LKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 29.1 bits (62), Expect = 0.90
Identities = 14/30 (46%), Positives = 15/30 (50%)
Frame = +2
Query: 686 PPPPPXKKKKXXPPPXGXXXPPPPPXXKKK 775
PPPPP + PPP PPPP KK
Sbjct: 12 PPPPPGFEPPSQPPP------PPPPGYVKK 35
Score = 28.3 bits (60), Expect = 1.6
Identities = 13/30 (43%), Positives = 14/30 (46%)
Frame = +2
Query: 686 PPPPPXKKKKXXPPPXGXXXPPPPPXXKKK 775
PPPPP PP PPPP KK+
Sbjct: 9 PPPPPPPP--GFEPPSQPPPPPPPGYVKKR 36
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.5 bits (58), Expect = 2.7
Identities = 16/58 (27%), Positives = 17/58 (29%)
Frame = +3
Query: 585 PPXXPPXXXFLKKXPPPPXGXFFFXXXXKKKXXXPPPPXXKKKKXXPPPXGXXPPPPP 758
P PP PP P K PP P + P P PP PP
Sbjct: 1155 PSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPP 1212
Score = 27.1 bits (57), Expect = 3.6
Identities = 14/45 (31%), Positives = 15/45 (33%)
Frame = +2
Query: 626 PPPPXGXFFFFXXXKKKXXXPPPPPXKKKKXXPPPXGXXXPPPPP 760
PP P K PP PP + P P P PPP
Sbjct: 1169 PPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPP 1213
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.1 bits (57), Expect = 3.6
Identities = 9/22 (40%), Positives = 11/22 (50%)
Frame = +2
Query: 695 PPXKKKKXXPPPXGXXXPPPPP 760
PP + + PP PPPPP
Sbjct: 1690 PPVRPQSAAPPQMSAPTPPPPP 1711
Score = 26.6 bits (56), Expect = 4.8
Identities = 12/28 (42%), Positives = 12/28 (42%), Gaps = 3/28 (10%)
Frame = +2
Query: 686 PPPPPXK---KKKXXPPPXGXXXPPPPP 760
PPPPP P P G PPPP
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 25.8 bits (54), Expect = 8.4
Identities = 24/72 (33%), Positives = 25/72 (34%), Gaps = 6/72 (8%)
Frame = -2
Query: 769 FXXXGGGGGXXPXGGGXXFFFFXXGG-----GGXXXFFFXXXKKKKXPXG-GGGXFFKKX 608
F GGG G P G G F GG GG F + P G GGG
Sbjct: 190 FGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGG 249
Query: 607 XXGGXXGGGXXF 572
GG GG F
Sbjct: 250 GLGGFGGGPGGF 261
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,265,827
Number of Sequences: 5004
Number of extensions: 40034
Number of successful extensions: 187
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 32
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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