Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP09_F_L22
(888 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 262 6e-71
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 260 2e-70
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 58 2e-09
SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 39 0.001
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 36 0.006
SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces pombe... 32 0.13
SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 28 2.0
SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr... 27 3.6
SPAC644.06c |cdr1|nim1|GIN4 family protein kinase Cdr1|Schizosac... 26 6.2
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 262 bits (641), Expect = 6e-71
Identities = 117/213 (54%), Positives = 146/213 (68%)
Frame = +3
Query: 87 TVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPEGV 266
T GK+I C AAVAW+ PLSIE ++V PP+ EVR+KI +GVCHTDAYTLSGKDPEG+
Sbjct: 6 TAGKIINCKAAVAWQPAAPLSIENVQVFPPRVHEVRIKIVNSGVCHTDAYTLSGKDPEGL 65
Query: 267 FPVVLXXXXXXXXXXXXXXXTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQ 446
FPV+L T+V+ GD V+ LY P+C TCKFC + KTNLC ++R+TQG+
Sbjct: 66 FPVILGHEGAGIVESVGPQVTTVQVGDPVIALYTPECKTCKFCKSGKTNLCGRIRTTQGK 125
Query: 447 GVMPDGTRRFRCKGQELYHFMGCSTFSQYTVVLXISLCKVAXAAPLXKVCXLGCGVPTGY 626
G+MPDGT RF C G L HFMGCSTFS+YTVV IS+ + APL VC LGCG+ TGY
Sbjct: 126 GLMPDGTSRFSCNGNTLLHFMGCSTFSEYTVVADISVVAIERLAPLDSVCLLGCGITTGY 185
Query: 627 GXALNTAKVEPXSNCAIXGLGAVGXXVAXGCXR 725
G A TA ++ + A+ GLG+VG V G +
Sbjct: 186 GAATITADIKEGDSVAVFGLGSVGLAVIQGAVK 218
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 260 bits (637), Expect = 2e-70
Identities = 119/212 (56%), Positives = 145/212 (68%)
Frame = +3
Query: 81 MSTVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPE 260
MS GK I C AAVAW A +PLSIE+I+V PPKA EVRVK+ + VCHTDAYTLSG DPE
Sbjct: 1 MSFEGKTITCKAAVAWGAKEPLSIEDIQVAPPKAHEVRVKVDWSAVCHTDAYTLSGVDPE 60
Query: 261 GVFPVVLXXXXXXXXXXXXXXXTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQ 440
G FP+VL +V+PGDHV+ LY P+C CKFC + KTNLC K+R TQ
Sbjct: 61 GAFPIVLGHEGAGIVESIGEGVINVRPGDHVILLYTPECKECKFCRSGKTNLCSKIRETQ 120
Query: 441 GQGVMPDGTRRFRCKGQELYHFMGCSTFSQYTVVLXISLCKVAXAAPLXKVCXLGCGVPT 620
G+G+MPDGT RF C+ + L H+MGCS+FSQYTVV ISL ++ +APL +C LGCGV T
Sbjct: 121 GRGLMPDGTSRFSCRDKTLLHYMGCSSFSQYTVVADISLVAISHSAPLRSICLLGCGVTT 180
Query: 621 GYGXALNTAKVEPXSNCAIXGLGAVGXXVAXG 716
G+G ++AKVE S A+ G G VG G
Sbjct: 181 GFGAVTHSAKVESGSTVAVVGCGCVGLAAMQG 212
>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
Adh1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 350
Score = 57.6 bits (133), Expect = 2e-09
Identities = 42/130 (32%), Positives = 56/130 (43%), Gaps = 4/130 (3%)
Frame = +3
Query: 105 KCLAAVAWEAGKPLSI--EEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDP-EGVFPV 275
K LAAV G P ++ EE+ V P EV V I TGVCHTD + L G P P+
Sbjct: 6 KQLAAVFHTHGGPENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQGDWPLPAKMPL 65
Query: 276 VLXXXXXXXXXXXXXXXTSVKPGDHV-VPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQGV 452
+ T +K GD V V C C++C+ + +C + Q G
Sbjct: 66 IGGHEGAGVVVKVGAGVTRLKIGDRVGVKWMNSSCGNCEYCMKAEETICPHI---QLSGY 122
Query: 453 MPDGTRRFRC 482
DGT + C
Sbjct: 123 TVDGTFQHYC 132
>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 360
Score = 38.7 bits (86), Expect = 0.001
Identities = 24/81 (29%), Positives = 37/81 (45%), Gaps = 3/81 (3%)
Frame = +3
Query: 186 EVRVKITATGVCHTDA-YTLSGKDPEGVF--PVVLXXXXXXXXXXXXXXXTSVKPGDHVV 356
+V+V I ATG+C +D Y G + + P++L +S+KPGD V
Sbjct: 31 QVKVAIKATGICGSDVHYWKEGGIGDFILKKPMILGHESAGVVVEVGKGVSSLKPGDPVA 90
Query: 357 PLYVPQCNTCKFCLNPKTNLC 419
C C +C + + NLC
Sbjct: 91 VEPGCVCRLCDYCRSGRYNLC 111
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 36.3 bits (80), Expect = 0.006
Identities = 45/202 (22%), Positives = 75/202 (37%), Gaps = 6/202 (2%)
Frame = +3
Query: 111 LAAVAWEAGKPLSIEEIEVDPPKAG---EVRVKITATGVCH-TDAYTLSGKDPEGVFPVV 278
+ A W+ PL+++ EV P +V VK TA +C +D++ SG+ P +
Sbjct: 37 MKACVWDG--PLNVKIAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPGIEKGAI 94
Query: 279 LXXXXXXXXXXXXXXXTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQGVMP 458
L +++ GD VV + C C FC + C ++ V
Sbjct: 95 LGHESCGIVAEKGDEVNNLEIGDRVVIAFDLACGQCSFCKRHEYAACDTTNDSKLMDV-N 153
Query: 459 DGTRRFRCKG--QELYHFMGCSTFSQYTVVLXISLCKVAXAAPLXKVCXLGCGVPTGYGX 632
G+ G + L GC I+ CK+ P + + + T
Sbjct: 154 YGSHHSAIFGYTKLLGDVPGCQAEYIRVPFAEINCCKLPDDIPDSEGLFMSDVLCTSL-H 212
Query: 633 ALNTAKVEPXSNCAIXGLGAVG 698
A +V+ AI G+G +G
Sbjct: 213 ACTLGEVKKGDTVAIWGMGPIG 234
>SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 325
Score = 31.9 bits (69), Expect = 0.13
Identities = 14/46 (30%), Positives = 25/46 (54%)
Frame = +3
Query: 144 LSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPEGVFPVVL 281
+ I+ + + PK GE+ VKI A + +D +G P V+P ++
Sbjct: 23 IEIQSVPIPQPKNGELLVKIEAAAINPSDLMNATGGFPYTVYPRIV 68
>SPCC1235.05c |fft2||fun thirty related protein
Fft2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1284
Score = 27.9 bits (59), Expect = 2.0
Identities = 15/46 (32%), Positives = 22/46 (47%)
Frame = +3
Query: 15 PLTIGNP*XLIFFVDWQLSSAVMSTVGKVIKCLAAVAWEAGKPLSI 152
P+T +P +I V W S + + +VI LA W+ LSI
Sbjct: 251 PVTTTSPNAIIRSVQWIRSFVPQAPIHQVINTLAQTKWDETAALSI 296
>SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1250
Score = 27.1 bits (57), Expect = 3.6
Identities = 18/53 (33%), Positives = 24/53 (45%), Gaps = 1/53 (1%)
Frame = +3
Query: 342 GDHVVPLY-VPQCNTCKFCLNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQEL 497
G HVV L V N C + KT KV + + P G+R++ C G L
Sbjct: 1036 GKHVVRLLQVRDANGCAASIT-KTQPAAKVSVVEMASLAPLGSRQYYCVGDRL 1087
>SPAC644.06c |cdr1|nim1|GIN4 family protein kinase
Cdr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 593
Score = 26.2 bits (55), Expect = 6.2
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = -3
Query: 184 PAFGGSTSISSMDSGLPASHATAAKHLITLPTVDMTALDNCQSTKKI 44
P G TS+SSMDS P + + + + L M L S+KK+
Sbjct: 256 PFLMGCTSLSSMDSTTPPTPSLSIDEIDPLVVDCMCVLWKKSSSKKV 302
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,785,773
Number of Sequences: 5004
Number of extensions: 53403
Number of successful extensions: 123
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 122
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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