BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP09_F_L21
(1351 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 33 0.068
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 32 0.21
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 2.5
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 3.4
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 23 5.9
SPAC18G6.12c |||hypothetical protein|Schizosaccharomyces pombe|c... 27 5.9
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 33.5 bits (73), Expect = 0.068
Identities = 17/49 (34%), Positives = 17/49 (34%), Gaps = 4/49 (8%)
Frame = +1
Query: 631 PPPPP----PXXGVGXFPXPPPXXXGGXXPXXPXXXXXGGXXXXXPPXP 765
PPPPP P P PPP G P P G PP P
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 31.9 bits (69), Expect = 0.21
Identities = 13/33 (39%), Positives = 13/33 (39%)
Frame = +3
Query: 867 PPPXXXXXXXXXPPXXPXPXXXXKTXPPPPPPP 965
P P PP P P PPPPPPP
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 30.3 bits (65), Expect = 0.64
Identities = 12/32 (37%), Positives = 12/32 (37%)
Frame = +2
Query: 869 PPXXXXXXSXXPPXXXXPPXXXKNXPPPPPPP 964
PP P PP PPPPPPP
Sbjct: 736 PPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPP 767
Score = 29.5 bits (63), Expect = 1.1
Identities = 12/32 (37%), Positives = 12/32 (37%)
Frame = +3
Query: 867 PPPXXXXXXXXXPPXXPXPXXXXKTXPPPPPP 962
PPP PP P PPPPPP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 29.1 bits (62), Expect = 1.5
Identities = 16/47 (34%), Positives = 16/47 (34%), Gaps = 3/47 (6%)
Frame = +1
Query: 586 PXPPXXFXXXXKXXXP---PPPPPXXGVGXFPXPPPXXXGGXXPXXP 717
P PP P PPP P G P PPP G P P
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP 779
Score = 29.1 bits (62), Expect = 1.5
Identities = 12/32 (37%), Positives = 12/32 (37%)
Frame = +3
Query: 870 PPXXXXXXXXXPPXXPXPXXXXKTXPPPPPPP 965
PP PP P PPPPPPP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 28.7 bits (61), Expect = 1.9
Identities = 16/51 (31%), Positives = 16/51 (31%)
Frame = +3
Query: 867 PPPXXXXXXXXXPPXXPXPXXXXKTXPPPPPPPXXXXPXPXXXXXXXPPXP 1019
PPP P P P PPPPPP P PP P
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPP---PPGVAGAGPPPPPPP 781
Score = 27.9 bits (59), Expect = 3.4
Identities = 10/18 (55%), Positives = 10/18 (55%)
Frame = +3
Query: 630 PPPPPPXXRGGXFSXPPP 683
PPPPPP G PPP
Sbjct: 763 PPPPPPGVAGAGPPPPPP 780
Score = 27.5 bits (58), Expect = 4.5
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +3
Query: 948 PPPPPPXXXXPXPXXXXXXXPP 1013
PPPPPP P P PP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPP 753
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 31.9 bits (69), Expect = 0.21
Identities = 11/21 (52%), Positives = 12/21 (57%)
Frame = +3
Query: 903 PPXXPXPXXXXKTXPPPPPPP 965
PP P P + PPPPPPP
Sbjct: 10 PPPPPPPGFEPPSQPPPPPPP 30
Score = 27.9 bits (59), Expect = 3.4
Identities = 10/21 (47%), Positives = 10/21 (47%)
Frame = +3
Query: 903 PPXXPXPXXXXKTXPPPPPPP 965
PP P P PPPPPP
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPP 29
Score = 27.5 bits (58), Expect = 4.5
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = +1
Query: 631 PPPPPPXXGVGXFPXPPPXXXGG 699
PPPPPP G PPP G
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPPG 31
Score = 27.1 bits (57), Expect = 5.9
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = +2
Query: 938 NXPPPPPPPXXXXP 979
N PPPPPPP P
Sbjct: 8 NPPPPPPPPGFEPP 21
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 27.1 bits (57), Expect = 5.9
Identities = 13/32 (40%), Positives = 13/32 (40%)
Frame = +2
Query: 869 PPXXXXXXSXXPPXXXXPPXXXKNXPPPPPPP 964
PP S P P N PPPPPPP
Sbjct: 167 PPPSFQPPSAAAPATSLPSDY--NPPPPPPPP 196
Score = 24.2 bits (50), Expect(2) = 2.5
Identities = 9/21 (42%), Positives = 9/21 (42%)
Frame = +3
Query: 903 PPXXPXPXXXXKTXPPPPPPP 965
PP PPPPPPP
Sbjct: 224 PPTYTPKQADPLPAPPPPPPP 244
Score = 22.2 bits (45), Expect(2) = 2.5
Identities = 7/11 (63%), Positives = 7/11 (63%)
Frame = +3
Query: 948 PPPPPPXXXXP 980
PPPPPP P
Sbjct: 238 PPPPPPPTLPP 248
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.9 bits (59), Expect = 3.4
Identities = 26/117 (22%), Positives = 29/117 (24%), Gaps = 7/117 (5%)
Frame = +3
Query: 630 PPPPPPXXRGGXFSX-------PPPXXXXGGXXXXXPXXXGGXXXXXXPPXPXXXXXXXX 788
PP PPP R + PPP P G PP P
Sbjct: 290 PPLPPPSSRVSAAALAANKKRPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAG 349
Query: 789 XXXXXXXXXXXXXXXXXXXXXXXGRXPPPXXXXXXXXXPPXXPXPXXXXKTXPPPPP 959
GR PPP PP P P ++ P PP
Sbjct: 350 SIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPP-APPPAIPGRSAPALPP 405
Score = 27.5 bits (58), Expect = 4.5
Identities = 14/44 (31%), Positives = 14/44 (31%)
Frame = +1
Query: 586 PXPPXXFXXXXKXXXPPPPPPXXGVGXFPXPPPXXXGGXXPXXP 717
P PP PPP V P PPP G P P
Sbjct: 361 PPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALP 404
Score = 27.1 bits (57), Expect = 5.9
Identities = 14/43 (32%), Positives = 14/43 (32%), Gaps = 1/43 (2%)
Frame = +1
Query: 556 PXXXPXFXXXPXPPXXFXXXXKXXXPPPPPP-XXGVGXFPXPP 681
P P PP PPPPPP G P PP
Sbjct: 313 PPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPP 355
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 23.0 bits (47), Expect(2) = 5.9
Identities = 9/24 (37%), Positives = 9/24 (37%)
Frame = +3
Query: 948 PPPPPPXXXXPXPXXXXXXXPPXP 1019
PPPPPP P P P
Sbjct: 240 PPPPPPRPSQPTSGESQNTNPRFP 263
Score = 22.2 bits (45), Expect(2) = 5.9
Identities = 7/14 (50%), Positives = 8/14 (57%)
Frame = +3
Query: 921 PXXXXKTXPPPPPP 962
P + PPPPPP
Sbjct: 232 PIFSSSSTPPPPPP 245
>SPAC18G6.12c |||hypothetical protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 309
Score = 27.1 bits (57), Expect = 5.9
Identities = 12/36 (33%), Positives = 13/36 (36%)
Frame = -3
Query: 413 PXXFFXXXGXXGVFXFXXXIFXPPPPXPXKKKXRGW 306
P +F G F IF P PP P GW
Sbjct: 61 PWRYFTDRGFVVEFVIAESIFPPRPPKPNDSYMTGW 96
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.312 0.156 0.567
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,464,098
Number of Sequences: 5004
Number of extensions: 37386
Number of successful extensions: 457
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 35
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 214
length of database: 2,362,478
effective HSP length: 75
effective length of database: 1,987,178
effective search space used: 743204572
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.6 bits)
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