Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP09_F_K22
(891 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|... 210 2e-55
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase... 207 2e-54
SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange facto... 29 1.2
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate... 27 2.7
SPCC569.08c |ade5|ade8|phosphoribosylglycinamide formyltransfera... 27 3.6
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 26 8.3
>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 335
Score = 210 bits (514), Expect = 2e-55
Identities = 104/156 (66%), Positives = 117/156 (75%)
Frame = +2
Query: 317 LFVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSA 496
L ++G+ I V +ERDP I W +GAEYV+ESTGVFTT + ASAHL+GGAK+VIISAPS
Sbjct: 67 LVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAPSK 126
Query: 497 DAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXXXX 676
DAPMFVVGVNLE ++PS KVISNASCTTNCLAPLAKVI+D F I EGLM
Sbjct: 127 DAPMFVVGVNLEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGLMTTVHATTATQK 186
Query: 677 XXDGPSGKLWRDGRGAQQNIIPASTGAAKXVGXXXP 784
DGPS K WR GRGA NIIP+STGAAK VG P
Sbjct: 187 TVDGPSKKDWRGGRGASANIIPSSTGAAKAVGKVIP 222
Score = 99 bits (238), Expect = 4e-22
Identities = 47/64 (73%), Positives = 54/64 (84%), Gaps = 1/64 (1%)
Frame = +1
Query: 133 KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 309
K+GINGFGRIGR+VLR +I G QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE +
Sbjct: 5 KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64
Query: 310 GFLV 321
G LV
Sbjct: 65 GKLV 68
>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 336
Score = 207 bits (505), Expect = 2e-54
Identities = 102/156 (65%), Positives = 116/156 (74%)
Frame = +2
Query: 317 LFVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSA 496
L ++GN I V +ERDP I W +GA+YV+ESTGVFTT + ASAHL+GGAK+VIISAPS
Sbjct: 67 LVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAPSK 126
Query: 497 DAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXXXX 676
DAPM+VVGVN E ++PS KVISNASCTTNCLAPLAKVI+D F I EGLM
Sbjct: 127 DAPMYVVGVNEEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGLMTTVHATTATQK 186
Query: 677 XXDGPSGKLWRDGRGAQQNIIPASTGAAKXVGXXXP 784
DGPS K WR GRGA NIIP+STGAAK VG P
Sbjct: 187 TVDGPSKKDWRGGRGASANIIPSSTGAAKAVGKVIP 222
Score = 102 bits (245), Expect = 6e-23
Identities = 48/64 (75%), Positives = 55/64 (85%), Gaps = 1/64 (1%)
Frame = +1
Query: 133 KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 309
K+GINGFGRIGR+VLR A + K QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++D
Sbjct: 5 KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64
Query: 310 GFLV 321
G LV
Sbjct: 65 GKLV 68
>SPAC26F1.01 |sec74|SPAPJ691.01c|guanyl-nucleotide exchange factor
Sec74|Schizosaccharomyces pombe|chr 1|||Manual
Length = 928
Score = 28.7 bits (61), Expect = 1.2
Identities = 18/75 (24%), Positives = 33/75 (44%)
Frame = -1
Query: 531 SRLTPTTNMGASALGADIITFLAPPSK*ADALSVVVKTPVDSTTYSAPAFPHGMALGSLS 352
S+L+PT + L A ++ S ++ +DS+TY P ++ SLS
Sbjct: 174 SQLSPTHTSTIAELAASTLSIFPSGSYAGSTFGSPSRSSIDSSTY----LPRSKSVNSLS 229
Query: 351 EKTAILLPLTNKESI 307
+ P +N+ S+
Sbjct: 230 SNFSARTPASNQSSV 244
>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
transferase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 453
Score = 27.5 bits (58), Expect = 2.7
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = -2
Query: 191 SIEARSTKRPMRPNPLIPIFDMLSASYRKFVE*LTTTTYAQVQDG 57
++ A+ TK P+RP + P + SA + ++ + T AQVQ+G
Sbjct: 179 ALAAKLTK-PIRPLWVTPASRLTSAQFEEYFTVVLVTASAQVQNG 222
>SPCC569.08c |ade5|ade8|phosphoribosylglycinamide
formyltransferase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 207
Score = 27.1 bits (57), Expect = 3.6
Identities = 14/26 (53%), Positives = 18/26 (69%), Gaps = 1/26 (3%)
Frame = -1
Query: 339 ILLPL-TNKESILNLNTALKTAMGGI 265
+L+PL TNK I+NL+ AL A GI
Sbjct: 107 VLIPLETNKIGIINLHPALPGAFNGI 132
>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1583
Score = 25.8 bits (54), Expect = 8.3
Identities = 18/51 (35%), Positives = 27/51 (52%), Gaps = 3/51 (5%)
Frame = -1
Query: 327 LTNKESILNLNTALKTAMGGIILEKINHIVK---TDERVIYSDHLSSLFNR 184
L N+ESIL+L L + + E I++IV+ +D R HLS L +
Sbjct: 342 LQNEESILHLLNILHSIFEYTVPEAIDNIVQSKTSDARTSEIQHLSVLLQK 392
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,409,617
Number of Sequences: 5004
Number of extensions: 71028
Number of successful extensions: 208
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 201
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 206
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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