Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP09_F_I03
(884 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1604.08c |imp1||importin alpha|Schizosaccharomyces pombe|chr... 52 8e-08
SPCC962.03c |cut15||karyopherin Cut15|Schizosaccharomyces pombe|... 52 1e-07
SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pom... 30 0.50
SPBC3E7.01 |fab1|ste12, SPBC6B1.11c|1-phosphatidylinositol-3-pho... 28 1.5
SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyc... 27 2.7
SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac... 27 2.7
SPCC16A11.17 |cdc21|mcm4, SPCC24B10.01|MCM complex subunit Cdc21... 27 3.6
SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pom... 27 3.6
SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 27 4.7
SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosacc... 26 6.2
>SPBC1604.08c |imp1||importin alpha|Schizosaccharomyces pombe|chr
2|||Manual
Length = 539
Score = 52.4 bits (120), Expect = 8e-08
Identities = 25/54 (46%), Positives = 33/54 (61%)
Frame = +1
Query: 676 ADNPXLQFXTAWALTXIASGTSAQTNXVXXAGAAPVSLXPLXSPHDPVRDPALW 837
+++ LQF AWALT IASGT+ QT V +GA P + L SP VR+ +W
Sbjct: 131 SEHHLLQFEAAWALTNIASGTTDQTRIVVDSGAVPRFIQLLSSPEKDVREQVVW 184
Score = 48.4 bits (110), Expect = 1e-06
Identities = 28/67 (41%), Positives = 39/67 (58%), Gaps = 4/67 (5%)
Frame = +2
Query: 308 MATDQAKNRMHVFKNAGK-DVDEMRRRRNEVTVELRKNKREETLQKRRNVPISYSTD--- 475
M + +R FK+ G DE+RR+R E +E+RK KREE+L KRRN+ D
Sbjct: 1 MESRYLSDRRSRFKSKGVFKADELRRQREEQQIEIRKQKREESLNKRRNLNAVLQNDIDV 60
Query: 476 EEEIDKN 496
EEE D++
Sbjct: 61 EEEADQS 67
Score = 36.7 bits (81), Expect = 0.004
Identities = 16/35 (45%), Positives = 26/35 (74%)
Frame = +3
Query: 543 AENPEAQLAAVQQCRKLLSCDXNPPIDELIAAGIL 647
+++ E QL AV + RK LS + +PPID++IA G++
Sbjct: 88 SDDIELQLGAVTKFRKYLSKETHPPIDQVIACGVV 122
>SPCC962.03c |cut15||karyopherin Cut15|Schizosaccharomyces pombe|chr
3|||Manual
Length = 542
Score = 51.6 bits (118), Expect = 1e-07
Identities = 28/63 (44%), Positives = 37/63 (58%)
Frame = +1
Query: 649 PILVXCLSRADNPXLQFXTAWALTXIASGTSAQTNXVXXAGAAPVSLXPLXSPHDPVRDP 828
P V LS +N L+F +WALT +ASG+S QT+ V A A PV + L S VR+
Sbjct: 124 PRFVEFLSHENN-LLKFEASWALTNVASGSSNQTHVVVEANAVPVFVSLLSSSEQDVREQ 182
Query: 829 ALW 837
A+W
Sbjct: 183 AVW 185
Score = 44.8 bits (101), Expect = 2e-05
Identities = 24/55 (43%), Positives = 31/55 (56%), Gaps = 1/55 (1%)
Frame = +2
Query: 326 KNRMHVFKNAGK-DVDEMRRRRNEVTVELRKNKREETLQKRRNVPISYSTDEEEI 487
++R +K G DE+RRRR +E+RK KREE L KRRN+ EE I
Sbjct: 10 EHRRQNYKGKGTFQADELRRRRETQQIEIRKQKREENLNKRRNLVDVQEPAEETI 64
Score = 38.7 bits (86), Expect = 0.001
Identities = 17/42 (40%), Positives = 29/42 (69%)
Frame = +3
Query: 525 VMNAANAENPEAQLAAVQQCRKLLSCDXNPPIDELIAAGILP 650
++ A +++ EAQ+ A + RK LS + NPPI ++I AG++P
Sbjct: 83 LLKALYSDDIEAQIQATAKFRKALSKETNPPIQKVIDAGVVP 124
Score = 30.3 bits (65), Expect = 0.38
Identities = 15/38 (39%), Positives = 17/38 (44%)
Frame = +1
Query: 724 IASGTSAQTNXVXXAGAAPVSLXPLXSPHDPVRDPALW 837
I +G QT + GA L L SP D VR A W
Sbjct: 317 IVTGDDVQTQVIINCGALSALLSLLSSPRDGVRKEACW 354
>SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 649
Score = 29.9 bits (64), Expect = 0.50
Identities = 24/82 (29%), Positives = 41/82 (50%), Gaps = 9/82 (10%)
Frame = +2
Query: 290 TASTDKMATDQ--AKNRMHVFKN-AGKDVDE---MRRR---RNEVTVELRKNKREETLQK 442
TAS ++ D +NR +N +G V+E + R+ R + ++ ++N+ T+QK
Sbjct: 261 TASLEEYLNDPDFEENRKQYLQNKSGSPVEETAILNRKPTLRKKKSIPKKQNESSSTIQK 320
Query: 443 RRNVPISYSTDEEEIDKNLATT 508
V S+ EEE K+L T
Sbjct: 321 ENTVQQEASSSEEEAVKSLPET 342
>SPBC3E7.01 |fab1|ste12,
SPBC6B1.11c|1-phosphatidylinositol-3-phosphate 5-kinase
Fab1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1932
Score = 28.3 bits (60), Expect = 1.5
Identities = 14/44 (31%), Positives = 20/44 (45%)
Frame = -2
Query: 355 CILEHMHAVFRLISRHFICARCLDTVRGNYTKKQRINIKLLSSK 224
C+ E +FRL +C C + NY++ Q N L SK
Sbjct: 93 CLKEAPGFIFRLQGSIKVCRPCASILVNNYSRSQLFNHSLNESK 136
>SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 682
Score = 27.5 bits (58), Expect = 2.7
Identities = 17/56 (30%), Positives = 26/56 (46%), Gaps = 2/56 (3%)
Frame = +2
Query: 323 AKNRMHVFKNAGKDVDEMRR--RRNEVTVELRKNKREETLQKRRNVPISYSTDEEE 484
A M V +G DVD ++E T+E K K E Q+R+ + T ++E
Sbjct: 447 ANGEMEVTFTSGFDVDNNANSSEKDETTIEKYKRKAAERKQRRKELRQLKKTKDDE 502
>SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 376
Score = 27.5 bits (58), Expect = 2.7
Identities = 12/41 (29%), Positives = 27/41 (65%)
Frame = +2
Query: 326 KNRMHVFKNAGKDVDEMRRRRNEVTVELRKNKREETLQKRR 448
++R+ ++ K++DE RR++ E+ +E ++ E+ L+K R
Sbjct: 294 ESRLQKMQDNAKELDESRRKKIEL-LEKKERDEEQFLEKER 333
>SPCC16A11.17 |cdc21|mcm4, SPCC24B10.01|MCM complex subunit
Cdc21|Schizosaccharomyces pombe|chr 3|||Manual
Length = 911
Score = 27.1 bits (57), Expect = 3.6
Identities = 17/81 (20%), Positives = 39/81 (48%), Gaps = 3/81 (3%)
Frame = +2
Query: 245 VDSLFLCVITTYCVETASTDKMATDQAKNRMHVFKNAGKDVDEMRRRRNEVTVELRK-NK 421
V SLF + ++ ++ TD + + ++A +DE+R+ +E ++++ +K
Sbjct: 430 VKSLFKTYVDVVHIKKQDKRRLGTDPSTLESDIAEDAALQIDEVRKISDEEVEKIQQVSK 489
Query: 422 REE--TLQKRRNVPISYSTDE 478
R++ + R P Y D+
Sbjct: 490 RDDIYDILSRSLAPSIYEMDD 510
>SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 447
Score = 27.1 bits (57), Expect = 3.6
Identities = 13/33 (39%), Positives = 21/33 (63%), Gaps = 2/33 (6%)
Frame = +3
Query: 249 ILCFFV*LPRT-VSRQRAQ-IKWRLIKRKTACM 341
IL F+ +PRT + + R + + WR+I R AC+
Sbjct: 400 ILASFLPIPRTKIHQMRVKHVHWRIINRVVACL 432
>SPCC417.08 |tef3||translation elongation factor
eEF3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1047
Score = 26.6 bits (56), Expect = 4.7
Identities = 11/48 (22%), Positives = 23/48 (47%)
Frame = +2
Query: 320 QAKNRMHVFKNAGKDVDEMRRRRNEVTVELRKNKREETLQKRRNVPIS 463
Q K F G +++ ++ + ELRK K+E +++ + +S
Sbjct: 996 QEKEEGDTFDAFGNKIEKAKKAKKLTGAELRKKKKERMARRKAGLEVS 1043
>SPBC19C2.11c |||mitochondrial outer membrane protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 26.2 bits (55), Expect = 6.2
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = -1
Query: 143 KGWSVSFKKSFYFREFMTLTCLIS 72
KGWS F +SFY R LT ++
Sbjct: 6 KGWSDQFSESFYERATTLLTAALN 29
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,190,379
Number of Sequences: 5004
Number of extensions: 61015
Number of successful extensions: 204
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 204
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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