Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP09_F_D16
(889 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_O61697 Cluster: Putative beta-ureidopropionase; n=1; Ma... 265 9e-70
UniRef50_Q6NP10 Cluster: LD13390p; n=7; Eukaryota|Rep: LD13390p ... 204 2e-51
UniRef50_Q9UBR1 Cluster: Beta-ureidopropionase; n=42; root|Rep: ... 199 7e-50
UniRef50_A7SG03 Cluster: Predicted protein; n=1; Nematostella ve... 194 2e-48
UniRef50_UPI0000DC0724 Cluster: ureidopropionase, beta; n=1; Rat... 132 2e-29
UniRef50_Q5L031 Cluster: Beta-alanine synthase; n=19; Bacteria|R... 93 1e-17
UniRef50_Q6AHZ8 Cluster: Putative uncharacterized protein DKFZp7... 76 1e-12
UniRef50_Q972L1 Cluster: 281aa long hypothetical beta-ureidoprop... 66 1e-09
UniRef50_Q9UYV8 Cluster: Beta ureidopropionase; n=4; Thermococca... 60 1e-07
UniRef50_Q1IQA8 Cluster: Nitrilase/cyanide hydratase and apolipo... 58 4e-07
UniRef50_Q1GTC5 Cluster: Nitrilase/cyanide hydratase and apolipo... 53 8e-06
UniRef50_Q54JM9 Cluster: Putative uncharacterized protein; n=1; ... 53 1e-05
UniRef50_Q97RA3 Cluster: Carbon-nitrogen hydrolase family protei... 52 2e-05
UniRef50_A7I2D9 Cluster: Hydrolase, carbon-nitrogen family; n=1;... 52 3e-05
UniRef50_Q0AX54 Cluster: N-carbamoyl-D-amino acid amidohydrolase... 51 3e-05
UniRef50_A6DKQ0 Cluster: Carbon-nitrogen hydrolase family protei... 51 5e-05
UniRef50_A4B9A7 Cluster: Probable hydratase; n=2; Bacteria|Rep: ... 50 6e-05
UniRef50_Q8RUF8 Cluster: AT5g12040/F14F18_210; n=9; Magnoliophyt... 50 6e-05
UniRef50_Q606Z9 Cluster: Hydrolase, carbon-nitrogen family; n=38... 50 8e-05
UniRef50_A6QC56 Cluster: Hydrolase; n=2; Bacteria|Rep: Hydrolase... 49 2e-04
UniRef50_Q2NHR0 Cluster: Predicted amidohydrolase; n=1; Methanos... 48 3e-04
UniRef50_A7GE66 Cluster: Hydrolase, carbon-nitrogen family; n=13... 48 4e-04
UniRef50_O59829 Cluster: Nitrilase; n=2; cellular organisms|Rep:... 48 4e-04
UniRef50_A3LZY2 Cluster: Aliphatic nitrilase; n=1; Pichia stipit... 48 4e-04
UniRef50_Q7M8G2 Cluster: HYDROLASE-Predicted amidohydrolase; n=5... 47 6e-04
UniRef50_A5C5V4 Cluster: Putative uncharacterized protein; n=1; ... 47 7e-04
UniRef50_A6CCK5 Cluster: Putative uncharacterized protein; n=1; ... 46 0.001
UniRef50_Q89413 Cluster: A78R protein; n=6; Chlorovirus|Rep: A78... 45 0.003
UniRef50_Q2AH52 Cluster: Nitrilase/cyanide hydratase and apolipo... 45 0.003
UniRef50_A4M5M1 Cluster: Nitrilase/cyanide hydratase and apolipo... 44 0.004
UniRef50_A2XD42 Cluster: Putative uncharacterized protein; n=2; ... 44 0.004
UniRef50_Q972X1 Cluster: 264aa long hypothetical beta-ureidoprop... 44 0.004
UniRef50_Q5KJU9 Cluster: Hydrolase, putative; n=1; Filobasidiell... 44 0.005
UniRef50_Q1PXD4 Cluster: Similar to N-carbamoyl-D-amino acid hyd... 43 0.012
UniRef50_A0QPL8 Cluster: Hydrolase, carbon-nitrogen family prote... 42 0.016
UniRef50_A6CFF3 Cluster: Putative nitrilase; n=1; Planctomyces m... 42 0.028
UniRef50_A3ZLM3 Cluster: Putative nitrilase; n=1; Blastopirellul... 42 0.028
UniRef50_Q6AMZ4 Cluster: Putative uncharacterized protein; n=1; ... 41 0.037
UniRef50_A6T2L9 Cluster: Nitrilase; n=1; Janthinobacterium sp. M... 41 0.048
UniRef50_UPI0000E472D9 Cluster: PREDICTED: similar to Ureidoprop... 40 0.085
UniRef50_Q5LLB2 Cluster: Nitrilase family protein; n=7; Bacteria... 40 0.085
UniRef50_Q8TPH5 Cluster: Carbon-nitrogen hydrolase; n=1; Methano... 40 0.085
UniRef50_Q5V604 Cluster: Nitrilase; n=2; Halobacteriaceae|Rep: N... 39 0.20
UniRef50_Q6RWQ0 Cluster: Nitrilase; n=3; uncultured organism|Rep... 38 0.26
UniRef50_Q2S196 Cluster: Hydrolase, carbon-nitrogen family; n=1;... 38 0.26
UniRef50_Q1AWK1 Cluster: Nitrilase/cyanide hydratase and apolipo... 38 0.34
UniRef50_Q44185 Cluster: N-carbamoyl-D-amino acid hydrolase; n=1... 38 0.34
UniRef50_A1HQ26 Cluster: Nitrilase/cyanide hydratase and apolipo... 37 0.60
UniRef50_A0TTW8 Cluster: Nitrilase/cyanide hydratase and apolipo... 37 0.60
UniRef50_A5GU42 Cluster: Nitrilase-related protein; n=1; Synecho... 36 1.0
UniRef50_Q8W0T9 Cluster: Putative uncharacterized protein SB35P0... 36 1.0
UniRef50_Q6RWN7 Cluster: Nitrilase; n=21; root|Rep: Nitrilase - ... 36 1.8
UniRef50_Q9KE11 Cluster: BH1047 protein; n=1; Bacillus haloduran... 36 1.8
UniRef50_Q4KB18 Cluster: Hydrolase, carbon-nitrogen family; n=2;... 36 1.8
UniRef50_A4WA35 Cluster: Nitrilase/cyanide hydratase and apolipo... 36 1.8
UniRef50_Q5MD29 Cluster: CtaJ; n=2; Cystobacteraceae|Rep: CtaJ -... 35 2.4
UniRef50_Q1AZG5 Cluster: Nitrilase; n=1; Rubrobacter xylanophilu... 35 2.4
UniRef50_A1VWX6 Cluster: Nitrilase; n=2; Comamonadaceae|Rep: Nit... 35 2.4
UniRef50_Q75TH8 Cluster: Putative uncharacterized protein GSB07;... 35 3.2
UniRef50_A2BNC1 Cluster: Predicted amidohydrolase; n=1; Hyperthe... 35 3.2
UniRef50_A2BKF1 Cluster: Predicted amidohydrolase; n=1; Hyperthe... 35 3.2
UniRef50_Q9ZMC7 Cluster: Putative; n=6; Campylobacterales|Rep: P... 34 4.2
UniRef50_Q6N746 Cluster: Nitrilase/cyanide hydratase and apolipo... 34 4.2
UniRef50_Q84FR7 Cluster: D-N-carbamoylase; n=1; Arthrobacter cry... 34 4.2
UniRef50_A7DA57 Cluster: Nitrilase/cyanide hydratase and apolipo... 34 4.2
UniRef50_Q2GWJ9 Cluster: Putative uncharacterized protein; n=1; ... 34 4.2
UniRef50_Q8TPH6 Cluster: Putative uncharacterized protein; n=1; ... 34 4.2
UniRef50_A0L7H1 Cluster: Nitrilase/cyanide hydratase and apolipo... 34 5.6
UniRef50_A5D6C3 Cluster: Putative uncharacterized protein; n=1; ... 33 7.4
UniRef50_A1HPP3 Cluster: Nitrilase/cyanide hydratase and apolipo... 33 7.4
UniRef50_Q6RWQ5 Cluster: Nitrilase; n=1; uncultured organism|Rep... 33 9.7
UniRef50_A6FX13 Cluster: Nitrilase/cyanide hydratase and apolipo... 33 9.7
UniRef50_A0QWL8 Cluster: Carbon-nitrogen hydrolase family protei... 33 9.7
>UniRef50_O61697 Cluster: Putative beta-ureidopropionase; n=1;
Manduca sexta|Rep: Putative beta-ureidopropionase -
Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm)
Length = 185
Score = 265 bits (650), Expect = 9e-70
Identities = 128/181 (70%), Positives = 144/181 (79%)
Frame = +1
Query: 112 THSLESIINNNLTGRXLEXFNXIHFGRRNNLEIKLKXSSIXXXXXXXXXXXXXXFPAKDE 291
T SLE+II NNL+GR L+ FN I++GR+N+LE+KLK SS+ FPAK E
Sbjct: 4 TQSLEAIIENNLSGRDLDEFNRIYYGRKNHLEVKLKDSSLAAAKEADFEVAAYAFPAKKE 63
Query: 292 QTRPPRIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXP 471
QTRPPRIVKVG++QHSI PTDRPVNEQKKAIF+KVKKIIDVAGQEGVNIICFQELWN P
Sbjct: 64 QTRPPRIVKVGVIQHSIGAPTDRPVNEQKKAIFDKVKKIIDVAGQEGVNIICFQELWNMP 123
Query: 472 FAFCTREKQPWCEFAESDEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVI 651
FAFCTREKQPWCEFAES E+GPTT FLRELA+KY+MVIVSSIL+ TAVVI
Sbjct: 124 FAFCTREKQPWCEFAESAEEGPTTRFLRELAMKYSMVIVSSILDVMRNMLISCGTTAVVI 183
Query: 652 S 654
S
Sbjct: 184 S 184
>UniRef50_Q6NP10 Cluster: LD13390p; n=7; Eukaryota|Rep: LD13390p -
Drosophila melanogaster (Fruit fly)
Length = 408
Score = 204 bits (498), Expect = 2e-51
Identities = 97/198 (48%), Positives = 128/198 (64%)
Frame = +1
Query: 118 SLESIINNNLTGRXLEXFNXIHFGRRNNLEIKLKXSSIXXXXXXXXXXXXXXFPAKDEQT 297
+L + +L L+ I +G + ++L S+ F A++EQT
Sbjct: 30 NLNDCLEKHLPPDELKEVKRILYGVEEDQTLELPTSAKDIAEQNGFDIKGYRFTAREEQT 89
Query: 298 RPPRIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFA 477
R RIV+VG +Q+SI +PT P+ +Q++AI+NKVK +I A + G NI+C QE W PFA
Sbjct: 90 RKRRIVRVGAIQNSIVIPTTAPIEKQREAIWNKVKTMIKAAAEAGCNIVCTQEAWTMPFA 149
Query: 478 FCTREKQPWCEFAESDEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISD 657
FCTREK PWCEFAE E+GPTT L ELA Y MVI+ SILERD +H + +WNTAVVIS+
Sbjct: 150 FCTREKFPWCEFAEEAENGPTTKMLAELAKAYNMVIIHSILERDMEHGETIWNTAVVISN 209
Query: 658 TGNVIGKHRKNXIPRVGD 711
+G +GKHRKN IPRVGD
Sbjct: 210 SGRYLGKHRKNHIPRVGD 227
Score = 72.9 bits (171), Expect = 1e-11
Identities = 30/45 (66%), Positives = 33/45 (73%)
Frame = +2
Query: 725 YYMXGTXGHPVFATRYGXIAVTICFGRXHVLXWMMFGQNGAEIVF 859
YYM G GHPVF T +G +AV IC+GR H WMMFG NGAEIVF
Sbjct: 233 YYMEGNTGHPVFETEFGKLAVNICYGRHHPQNWMMFGLNGAEIVF 277
>UniRef50_Q9UBR1 Cluster: Beta-ureidopropionase; n=42; root|Rep:
Beta-ureidopropionase - Homo sapiens (Human)
Length = 384
Score = 199 bits (486), Expect = 7e-50
Identities = 99/198 (50%), Positives = 127/198 (64%)
Frame = +1
Query: 118 SLESIINNNLTGRXLEXFNXIHFGRRNNLEIKLKXSSIXXXXXXXXXXXXXXFPAKDEQT 297
SLE + +L L+ + +G+ ++ L + F A +EQ
Sbjct: 8 SLEECLEKHLPLPDLQEVKRVLYGKELR-KLDLPREAFEAASREDFELQGYAFEAAEEQL 66
Query: 298 RPPRIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFA 477
R PRIV VG+VQ+ I +P + PV EQ A+ ++K I++VA GVNIICFQE W PFA
Sbjct: 67 RRPRIVHVGLVQNRIPLPANAPVAEQVSALHRRIKAIVEVAAMCGVNIICFQEAWTMPFA 126
Query: 478 FCTREKQPWCEFAESDEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISD 657
FCTREK PW EFAES EDGPTT F ++LA + MV+VS ILERD +H D+LWNTAVVIS+
Sbjct: 127 FCTREKLPWTEFAESAEDGPTTRFCQKLAKNHDMVVVSPILERDSEHGDVLWNTAVVISN 186
Query: 658 TGNVIGKHRKNXIPRVGD 711
+G V+GK RKN IPRVGD
Sbjct: 187 SGAVLGKTRKNHIPRVGD 204
Score = 70.9 bits (166), Expect = 4e-11
Identities = 28/45 (62%), Positives = 34/45 (75%)
Frame = +2
Query: 725 YYMXGTXGHPVFATRYGXIAVTICFGRXHVLXWMMFGQNGAEIVF 859
YYM G GHPVF T++G IAV IC+GR H L W+M+ NGAEI+F
Sbjct: 210 YYMEGNLGHPVFQTQFGRIAVNICYGRHHPLNWLMYSINGAEIIF 254
>UniRef50_A7SG03 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 359
Score = 194 bits (474), Expect = 2e-48
Identities = 94/198 (47%), Positives = 123/198 (62%)
Frame = +1
Query: 118 SLESIINNNLTGRXLEXFNXIHFGRRNNLEIKLKXSSIXXXXXXXXXXXXXXFPAKDEQT 297
SL + NL L+ I +G + ++ L +++ A E+
Sbjct: 7 SLNKTLEKNLPAEDLKEVKRILYGNPVS-DLSLPAAAVSVAAELDFELAGYKIDAAAEEL 65
Query: 298 RPPRIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFA 477
R PR+V++G VQ+ I PT+ P+ +Q++ + N++K I+ A VN+ICFQE W PFA
Sbjct: 66 RQPRLVRIGAVQNKIVEPTNMPIAKQREGLHNRMKDIVKAAALSKVNVICFQECWTMPFA 125
Query: 478 FCTREKQPWCEFAESDEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISD 657
FCTREKQPW EFAES EDGPT +E A +Y MVIVS ILERD H +ILWNTAV+IS+
Sbjct: 126 FCTREKQPWTEFAESAEDGPTVRLCQEWAKRYNMVIVSPILERDHTHQEILWNTAVIISN 185
Query: 658 TGNVIGKHRKNXIPRVGD 711
TG VIGK RKN IPRVGD
Sbjct: 186 TGEVIGKTRKNHIPRVGD 203
>UniRef50_UPI0000DC0724 Cluster: ureidopropionase, beta; n=1; Rattus
norvegicus|Rep: ureidopropionase, beta - Rattus
norvegicus
Length = 392
Score = 132 bits (318), Expect = 2e-29
Identities = 69/184 (37%), Positives = 101/184 (54%)
Frame = +1
Query: 118 SLESIINNNLTGRXLEXFNXIHFGRRNNLEIKLKXSSIXXXXXXXXXXXXXXFPAKDEQT 297
SLE + +L L I +G++ + L ++ F A EQ
Sbjct: 8 SLEQCLEKHLPPDDLSQVKRILYGKQTR-NLDLPRKALEAASERNFELKGYAFGAAKEQQ 66
Query: 298 RPPRIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFA 477
R P+IV+VG+VQ+ I +PT PV EQ A+ ++++I +VA GVNIICFQE WN PFA
Sbjct: 67 RCPQIVRVGLVQNRIPLPTSAPVAEQVSALHKRIEEIAEVAAMCGVNIICFQEAWNMPFA 126
Query: 478 FCTREKQPWCEFAESDEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISD 657
FCTREK PW EFAES EDG TT F ++ ++ + +++ L + + WN+ + +
Sbjct: 127 FCTREKLPWTEFAESAEDGLTTRFCQKGKFQHIVCLIAIFLRQSLTLGLVAWNSLDISVN 186
Query: 658 TGNV 669
G V
Sbjct: 187 AGLV 190
Score = 71.3 bits (167), Expect = 3e-11
Identities = 28/45 (62%), Positives = 34/45 (75%)
Frame = +2
Query: 725 YYMXGTXGHPVFATRYGXIAVTICFGRXHVLXWMMFGQNGAEIVF 859
YYM G GHPVF T++G IAV IC+GR H L W+M+ NGAEI+F
Sbjct: 209 YYMEGNLGHPVFQTQFGRIAVNICYGRHHPLNWLMYSVNGAEIIF 253
>UniRef50_Q5L031 Cluster: Beta-alanine synthase; n=19; Bacteria|Rep:
Beta-alanine synthase - Geobacillus kaustophilus
Length = 296
Score = 92.7 bits (220), Expect = 1e-17
Identities = 51/132 (38%), Positives = 71/132 (53%)
Frame = +1
Query: 313 VKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTRE 492
V +G++Q S V D PV K+ K K++ A G IIC QE++ P+ FC +
Sbjct: 5 VTIGLIQASHNVHGDEPVEVHKEKAIEKHVKLVKEAKDRGAQIICLQEIFYGPY-FCAEQ 63
Query: 493 KQPWCEFAESDEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVI 672
W E AE +GPTT +E+A + +VIV I ER+ + +NTA VI G +
Sbjct: 64 NTKWYEAAEEIPNGPTTKMFQEIAKQLGVVIVLPIYEREGIAT--YYNTAAVIDADGTYL 121
Query: 673 GKHRKNXIPRVG 708
GK+RK IP VG
Sbjct: 122 GKYRKQHIPHVG 133
Score = 37.1 bits (82), Expect = 0.60
Identities = 18/45 (40%), Positives = 23/45 (51%)
Frame = +2
Query: 725 YYMXGTXGHPVFATRYGXIAVTICFGRXHVLXWMMFGQNGAEIVF 859
Y+ G G+ VF T + I V IC+ R + G GAEIVF
Sbjct: 146 YFKPGNLGYSVFDTAFAKIGVYICYDRHFPEGARILGLKGAEIVF 190
>UniRef50_Q6AHZ8 Cluster: Putative uncharacterized protein
DKFZp779O1248; n=1; Homo sapiens|Rep: Putative
uncharacterized protein DKFZp779O1248 - Homo sapiens
(Human)
Length = 186
Score = 75.8 bits (178), Expect = 1e-12
Identities = 41/115 (35%), Positives = 60/115 (52%)
Frame = +1
Query: 118 SLESIINNNLTGRXLEXFNXIHFGRRNNLEIKLKXSSIXXXXXXXXXXXXXXFPAKDEQT 297
SLE + +L L+ + +G+ ++ L + F A +EQ
Sbjct: 8 SLEECLEKHLPLPDLQEVKRVLYGKELR-KLDLPREAFEAASREDFELQGYAFEAAEEQL 66
Query: 298 RPPRIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELW 462
R PRIV VG+VQ+ I +P + PV EQ A+ ++K I++VA GVNIICFQE W
Sbjct: 67 RRPRIVHVGLVQNRIPLPANAPVAEQVSALHRRIKAIVEVAAMCGVNIICFQEAW 121
>UniRef50_Q972L1 Cluster: 281aa long hypothetical
beta-ureidopropionase; n=1; Sulfolobus tokodaii|Rep:
281aa long hypothetical beta-ureidopropionase -
Sulfolobus tokodaii
Length = 281
Score = 65.7 bits (153), Expect = 1e-09
Identities = 38/112 (33%), Positives = 61/112 (54%)
Frame = +1
Query: 370 EQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXF 549
E K+A K + A ++G +I + EL+ + F E + + AE EDGPT
Sbjct: 16 ESKEANIQKALEYTKAAVKDGAELIVYNELFTTQY-FPATEDPKFFDLAEP-EDGPTVRV 73
Query: 550 LRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNXIPRV 705
E + +Y + ++ +I E D+K I ++TA+ I D G V+GK+RK IP+V
Sbjct: 74 FAEFSKQYKIGMIITIFEEDKKIKGIYYDTAIFIKD-GKVLGKYRKTHIPQV 124
>UniRef50_Q9UYV8 Cluster: Beta ureidopropionase; n=4;
Thermococcaceae|Rep: Beta ureidopropionase - Pyrococcus
abyssi
Length = 262
Score = 59.7 bits (138), Expect = 1e-07
Identities = 35/100 (35%), Positives = 59/100 (59%)
Frame = +1
Query: 388 FNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAI 567
++K +K+I A ++G ++ EL++ + F TRE+ E A+ +G TT FL ++A
Sbjct: 20 YSKAEKLIKEASKQGAQLVVLPELFDTGYNFETREEV--FEIAQKIPEGETTTFLMDVAR 77
Query: 568 KYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
+ IV+ E+D D+L+N+AVV+ G IGK+RK
Sbjct: 78 DTGVYIVAGTAEKD---GDVLYNSAVVVGPRG-FIGKYRK 113
>UniRef50_Q1IQA8 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=52; Bacteria|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Acidobacteria bacterium (strain
Ellin345)
Length = 303
Score = 57.6 bits (133), Expect = 4e-07
Identities = 43/133 (32%), Positives = 67/133 (50%)
Frame = +1
Query: 301 PPRIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAF 480
P +G++Q S PV E+ A + ++ D A Q G +IC EL+ + F
Sbjct: 2 PAEKFTIGLIQMSCG-----PVPEENMA--KALDRVRDAAKQ-GATVICLPELFQTQY-F 52
Query: 481 CTREKQPWCEFAESDEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDT 660
C RE E AES GP T + +LA + +V+V+S+ ER + + NTA ++ +
Sbjct: 53 CQREDTALFELAESIP-GPATKKMGDLARELGVVVVASLFER--RAPGLYHNTAAILDEA 109
Query: 661 GNVIGKHRKNXIP 699
G + G +RK IP
Sbjct: 110 GALKGIYRKMHIP 122
>UniRef50_Q1GTC5 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=9; Bacteria|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Sphingopyxis alaskensis
(Sphingomonas alaskensis)
Length = 300
Score = 53.2 bits (122), Expect = 8e-06
Identities = 31/101 (30%), Positives = 50/101 (49%)
Frame = +1
Query: 397 VKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKYA 576
V +++ A G II EL+ P+ FC E++ A + P+ ++ LA K
Sbjct: 42 VTALVEAAAARGAQIILPPELFEGPY-FCQVEEEELFATARPTAEHPSVVAMQALAAKCK 100
Query: 577 MVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNXIP 699
+ I +S ERD H +NT +I G ++G +RK+ IP
Sbjct: 101 VAIPTSFFERDGHH---YYNTLAMIGPDGGIMGTYRKSHIP 138
>UniRef50_Q54JM9 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 328
Score = 52.8 bits (121), Expect = 1e-05
Identities = 32/107 (29%), Positives = 57/107 (53%)
Frame = +1
Query: 367 NEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTX 546
+ +++ + N +K I D A + G +I E +N P++ T EK ++E+ EDG T
Sbjct: 64 DNKEENVQNAIKHI-DEAAKNGAKLISLPECFNSPYSTSTFEK-----YSET-EDGETVK 116
Query: 547 FLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
L E A + + +V + +K + ++NT + +D G V+ KHRK
Sbjct: 117 KLSEAAKRNQIFLVGGSIPEIDKATGKIYNTCFIFNDKGEVVKKHRK 163
>UniRef50_Q97RA3 Cluster: Carbon-nitrogen hydrolase family protein;
n=24; Bacteria|Rep: Carbon-nitrogen hydrolase family
protein - Streptococcus pneumoniae
Length = 291
Score = 52.0 bits (119), Expect = 2e-05
Identities = 27/100 (27%), Positives = 53/100 (53%)
Frame = +1
Query: 400 KKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKYAM 579
++++ A ++G II EL+ P+ FC + + ++A+S + + +A + +
Sbjct: 25 ERLVRQAAEQGAQIILLPELFEHPY-FCQERQYDYYQYAQSVAENTAIQHFKVIAKELQV 83
Query: 580 VIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNXIP 699
V+ S E+D ++L+N+ VI G V+G +RK IP
Sbjct: 84 VLPISFYEKD---GNVLYNSIAVIDADGEVLGVYRKTHIP 120
>UniRef50_A7I2D9 Cluster: Hydrolase, carbon-nitrogen family; n=1;
Campylobacter hominis ATCC BAA-381|Rep: Hydrolase,
carbon-nitrogen family - Campylobacter hominis (strain
ATCC BAA-381 / LMG 19568 / NCTC 13146 /CH001A)
Length = 336
Score = 51.6 bits (118), Expect = 3e-05
Identities = 30/102 (29%), Positives = 54/102 (52%)
Frame = +1
Query: 394 KVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKY 573
K ++I+ ++G ++ QEL + FC E+ FA ++ + F E A K+
Sbjct: 23 KSVEMIEKVAKDGAKLVILQELHEWAY-FCQSERVE--NFALAENFNESLKFWGETAKKF 79
Query: 574 AMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNXIP 699
+V+V+S+ E+ + + NTA+V + G + GK+RK IP
Sbjct: 80 GIVLVTSLFEK--RAPGLFHNTAIVFENNGEIAGKYRKMHIP 119
>UniRef50_Q0AX54 Cluster: N-carbamoyl-D-amino acid amidohydrolase;
n=1; Syntrophomonas wolfei subsp. wolfei str.
Goettingen|Rep: N-carbamoyl-D-amino acid amidohydrolase
- Syntrophomonas wolfei subsp. wolfei (strain
Goettingen)
Length = 283
Score = 51.2 bits (117), Expect = 3e-05
Identities = 32/99 (32%), Positives = 55/99 (55%), Gaps = 1/99 (1%)
Frame = +1
Query: 394 KVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKY 573
K ++I A EG ++ E++N P+ + + + +AE GP+T FL A K+
Sbjct: 24 KAGEMIAAAAGEGAEMVVLPEVFNSPY-----QAELFPRYAEPFP-GPSTDFLAAAACKH 77
Query: 574 AMVIVS-SILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
+ IV SI+ERD + ++N++ V + G +IG+HRK
Sbjct: 78 GLCIVGGSIIERDSQGK--IYNSSFVFDERGELIGRHRK 114
>UniRef50_A6DKQ0 Cluster: Carbon-nitrogen hydrolase family protein;
n=1; Lentisphaera araneosa HTCC2155|Rep: Carbon-nitrogen
hydrolase family protein - Lentisphaera araneosa
HTCC2155
Length = 286
Score = 50.8 bits (116), Expect = 5e-05
Identities = 31/100 (31%), Positives = 53/100 (53%)
Frame = +1
Query: 403 KIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKYAMV 582
K+I A + G NIIC QEL+ + FC + ++A+ + F ++ A + +V
Sbjct: 24 KLIADAAKSGANIICTQELFLSNY-FCREQNTEHFQYAQKIDQELLADF-QQCAKNHGVV 81
Query: 583 IVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNXIPR 702
+ S E E + + +NT+V+I G +GK+RK IP+
Sbjct: 82 LALSFFE--EALNGVYYNTSVIIDADGTYLGKYRKLHIPQ 119
>UniRef50_A4B9A7 Cluster: Probable hydratase; n=2; Bacteria|Rep:
Probable hydratase - Reinekea sp. MED297
Length = 289
Score = 50.4 bits (115), Expect = 6e-05
Identities = 27/100 (27%), Positives = 50/100 (50%)
Frame = +1
Query: 400 KKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKYAM 579
++++ A G +I QEL+ P+ FC +K+ + FA + +D P +A + +
Sbjct: 25 ERLVREAAASGAQVILLQELFERPY-FCQHQKEEFRRFATAIDDNPAIAHFAPIARELGV 83
Query: 580 VIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNXIP 699
V+ S E+ + +N+ VV+ G +G +RK IP
Sbjct: 84 VLPISFF---EQCGPVAYNSVVVLDADGENLGLYRKTHIP 120
>UniRef50_Q8RUF8 Cluster: AT5g12040/F14F18_210; n=9;
Magnoliophyta|Rep: AT5g12040/F14F18_210 - Arabidopsis
thaliana (Mouse-ear cress)
Length = 369
Score = 50.4 bits (115), Expect = 6e-05
Identities = 31/110 (28%), Positives = 53/110 (48%), Gaps = 2/110 (1%)
Frame = +1
Query: 364 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQP-WCEFAESDED-GP 537
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 538 TTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
+T L E++ + + I+ + E+ D L+NT V G + KHRK
Sbjct: 154 STAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHRK 201
>UniRef50_Q606Z9 Cluster: Hydrolase, carbon-nitrogen family; n=38;
Bacteria|Rep: Hydrolase, carbon-nitrogen family -
Methylococcus capsulatus
Length = 295
Score = 50.0 bits (114), Expect = 8e-05
Identities = 32/91 (35%), Positives = 51/91 (56%)
Frame = +1
Query: 427 EGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKYAMVIVSSILER 606
+G +++ EL P+ FC E + AE+ GPTT L +A + +V+V+S+ ER
Sbjct: 35 KGADLVMLPELHLGPY-FCQTEDCSCFDGAETIP-GPTTAELGSVARELGVVVVASLFER 92
Query: 607 DEKHSDILWNTAVVISDTGNVIGKHRKNXIP 699
+ + NTAVV+ G++ GK+RK IP
Sbjct: 93 --RAPGLYHNTAVVLDSDGSLAGKYRKMHIP 121
>UniRef50_A6QC56 Cluster: Hydrolase; n=2; Bacteria|Rep: Hydrolase -
Sulfurovum sp. (strain NBC37-1)
Length = 290
Score = 48.8 bits (111), Expect = 2e-04
Identities = 32/97 (32%), Positives = 51/97 (52%)
Frame = +1
Query: 409 IDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKYAMVIV 588
I+ A +I QEL + FC E + ++A +D D + F +A K+ +V+V
Sbjct: 25 IEEAASNSTELIVLQELHQNEY-FCQSEDTAFFDYA-ADFDADVS-FWGAVAKKHGIVLV 81
Query: 589 SSILERDEKHSDILWNTAVVISDTGNVIGKHRKNXIP 699
+S+ E+ + + NTAVV GN+ GK+RK IP
Sbjct: 82 TSLFEK--RAPGLYHNTAVVFEKDGNIAGKYRKMHIP 116
>UniRef50_Q2NHR0 Cluster: Predicted amidohydrolase; n=1;
Methanosphaera stadtmanae DSM 3091|Rep: Predicted
amidohydrolase - Methanosphaera stadtmanae (strain DSM
3091)
Length = 274
Score = 48.0 bits (109), Expect = 3e-04
Identities = 27/95 (28%), Positives = 50/95 (52%)
Frame = +1
Query: 403 KIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKYAMV 582
++I A G +I E++N P+ + + E+ E + T ++++A + +
Sbjct: 26 QLIKKASSNGAKLITLPEMFNTPY-----DNSKFIEYCEEETTSKTLNSMQDIAREENIY 80
Query: 583 IVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
+ S + EK S+ L+NTA +I+ G +IGKHRK
Sbjct: 81 LQSGSIP--EKESNHLYNTAYLINPKGKIIGKHRK 113
>UniRef50_A7GE66 Cluster: Hydrolase, carbon-nitrogen family; n=13;
cellular organisms|Rep: Hydrolase, carbon-nitrogen
family - Clostridium botulinum (strain Langeland / NCTC
10281 / Type F)
Length = 278
Score = 47.6 bits (108), Expect = 4e-04
Identities = 29/108 (26%), Positives = 54/108 (50%)
Frame = +1
Query: 364 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTT 543
V ++KK K +++ A +E NI E++N P+ + +P+ E + G T
Sbjct: 13 VQKEKKKNIKKAIEMLTKAKKENCNIAVLPEMFNCPYE--NKCFKPYGEIINEENGGETV 70
Query: 544 XFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
+++ A + IV+ + E D ++NT++V + G +I KHRK
Sbjct: 71 KAIKKAAKDLELYIVAGSIPEIE--GDKIYNTSMVFDNKGVLIAKHRK 116
>UniRef50_O59829 Cluster: Nitrilase; n=2; cellular organisms|Rep:
Nitrilase - Schizosaccharomyces pombe (Fission yeast)
Length = 272
Score = 47.6 bits (108), Expect = 4e-04
Identities = 27/84 (32%), Positives = 47/84 (55%)
Frame = +1
Query: 436 NIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKYAMVIVSSILERDEK 615
N+I F EL + C + + AE +GP+ + LA KY + I+ E++EK
Sbjct: 39 NLILFPELITSGYE-CGNT---FTQIAEIAGEGPSFKTMSNLAAKYHVNIIYGFPEKEEK 94
Query: 616 HSDILWNTAVVISDTGNVIGKHRK 687
S+I++N+ + I++ GN+ G +RK
Sbjct: 95 QSNIIYNSCIYITENGNLGGVYRK 118
>UniRef50_A3LZY2 Cluster: Aliphatic nitrilase; n=1; Pichia
stipitis|Rep: Aliphatic nitrilase - Pichia stipitis
(Yeast)
Length = 323
Score = 47.6 bits (108), Expect = 4e-04
Identities = 35/121 (28%), Positives = 53/121 (43%), Gaps = 8/121 (6%)
Frame = +1
Query: 361 PVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQP------WCEFAES 522
PV K+A KV + A +G N+I F E + F K P + + ES
Sbjct: 15 PVMMNKEATMEKVFNGVSEAASKGANLIVFPETYVSAFPLWGACKAPIDNHHLFKQLVES 74
Query: 523 DE--DGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNXI 696
DGP L+ L + ++V++ ER LWN+ V+I + G IG H + +
Sbjct: 75 SIYIDGPEISSLQSLCKELSVVVLLGFNERSRVSVGCLWNSYVLIDENG-TIGAHHRKLV 133
Query: 697 P 699
P
Sbjct: 134 P 134
>UniRef50_Q7M8G2 Cluster: HYDROLASE-Predicted amidohydrolase; n=5;
Bacteria|Rep: HYDROLASE-Predicted amidohydrolase -
Wolinella succinogenes
Length = 290
Score = 47.2 bits (107), Expect = 6e-04
Identities = 30/108 (27%), Positives = 55/108 (50%)
Frame = +1
Query: 376 KKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLR 555
++A + +++I A + G ++ QEL + FC E+ + ++A E+
Sbjct: 14 REATIQRSRELILEASKGGAELVVMQELHTSEY-FCQSEETRFFDYASFYEED--VRIFS 70
Query: 556 ELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNXIP 699
+A + +V+V S ER + + I NTAVV G++ G++RK IP
Sbjct: 71 SIAKEGGVVLVGSFFER--RSAGIYHNTAVVFEKDGSIAGRYRKMHIP 116
>UniRef50_A5C5V4 Cluster: Putative uncharacterized protein; n=1;
Vitis vinifera|Rep: Putative uncharacterized protein -
Vitis vinifera (Grape)
Length = 317
Score = 46.8 bits (106), Expect = 7e-04
Identities = 35/131 (26%), Positives = 67/131 (51%)
Frame = +1
Query: 307 RIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCT 486
R+V V +Q A D P N N ++++ A ++G NII QEL+ + FC
Sbjct: 5 RVVVVSALQ--FACTDDVPTN------LNTAERLVRDAHRKGANIILIQELFEG-YYFCQ 55
Query: 487 REKQPWCEFAESDEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGN 666
+++ + + A+ + PT +++LA + +VI S E+ ++ +N+ ++ G
Sbjct: 56 AQREDFFQRAKPYKGHPTILRMQKLAKELGVVIPVSFF---EEANNAHYNSIAIVDADGT 112
Query: 667 VIGKHRKNXIP 699
+G +RK+ IP
Sbjct: 113 DLGIYRKSHIP 123
>UniRef50_A6CCK5 Cluster: Putative uncharacterized protein; n=1;
Planctomyces maris DSM 8797|Rep: Putative
uncharacterized protein - Planctomyces maris DSM 8797
Length = 450
Score = 46.4 bits (105), Expect = 0.001
Identities = 26/66 (39%), Positives = 41/66 (62%), Gaps = 2/66 (3%)
Frame = +1
Query: 511 FAESDED--GPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHR 684
+AE+ E GP+T + ELA K+ + IV + ER + +++N AV+I G V+GK+R
Sbjct: 249 YAETAEPIPGPSTQYFGELAKKHDLYIVVGLYERA---AHLVYNVAVLIGPDGKVVGKYR 305
Query: 685 KNXIPR 702
K +PR
Sbjct: 306 KVTLPR 311
>UniRef50_Q89413 Cluster: A78R protein; n=6; Chlorovirus|Rep: A78R
protein - Paramecium bursaria Chlorella virus 1 (PBCV-1)
Length = 298
Score = 44.8 bits (101), Expect = 0.003
Identities = 25/103 (24%), Positives = 49/103 (47%)
Frame = +1
Query: 394 KVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKY 573
+ + ++ A G +I QEL+ + FC + + +FA+ +D +LA +
Sbjct: 24 RAEMLVRNAAANGAQVIVLQELFATKY-FCQTQSPQYFKFADPADDSVIVEIFSKLAKEL 82
Query: 574 AMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNXIPR 702
+VI E+D + +N+ V G+++G +RK IP+
Sbjct: 83 GVVIPIPFFEKDGNN---YYNSVAVADADGSIVGVYRKTHIPQ 122
>UniRef50_Q2AH52 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Halothermothrix
orenii H 168|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Halothermothrix
orenii H 168
Length = 273
Score = 44.8 bits (101), Expect = 0.003
Identities = 21/52 (40%), Positives = 30/52 (57%)
Frame = +1
Query: 532 GPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
G TT E A Y I+ +++ERD+ +IL+NT VI G+ GK+RK
Sbjct: 67 GRTTEIFSEYARMYKTAIIGNMVERDKNVGEILYNTTFVIDKKGDYTGKYRK 118
>UniRef50_A4M5M1 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Petrotoga mobilis
SJ95|Rep: Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Petrotoga mobilis SJ95
Length = 276
Score = 44.4 bits (100), Expect = 0.004
Identities = 22/58 (37%), Positives = 38/58 (65%)
Frame = +1
Query: 514 AESDEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
AE DG TT + +A KY + IV++ILE+D ++T+++I ++G ++GK+RK
Sbjct: 61 AEIIPDGETTQEVVRIAKKYNISIVANILEKDPLIIGKYYDTSILIDESGKLLGKYRK 118
>UniRef50_A2XD42 Cluster: Putative uncharacterized protein; n=2;
Oryza sativa|Rep: Putative uncharacterized protein -
Oryza sativa subsp. indica (Rice)
Length = 349
Score = 44.4 bits (100), Expect = 0.004
Identities = 29/112 (25%), Positives = 50/112 (44%), Gaps = 4/112 (3%)
Frame = +1
Query: 364 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDG--- 534
V K + ++ I+ A G ++ E+WN P++ + E+AE E G
Sbjct: 55 VTADKARNIARAREAIEAAAAGGAKLVLLPEIWNGPYS-----NDSFPEYAEDIEAGGDA 109
Query: 535 -PTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
P+ + E+A + +V + E+ + L+NT V G + GKHRK
Sbjct: 110 APSFSMMSEVARSLQITLVGGSIS--ERSGNKLYNTCCVFGSDGELKGKHRK 159
>UniRef50_Q972X1 Cluster: 264aa long hypothetical
beta-ureidopropionase; n=1; Sulfolobus tokodaii|Rep:
264aa long hypothetical beta-ureidopropionase -
Sulfolobus tokodaii
Length = 264
Score = 44.4 bits (100), Expect = 0.004
Identities = 31/109 (28%), Positives = 56/109 (51%)
Frame = +1
Query: 376 KKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLR 555
KK + ++++ A II EL N + F + + +AE+ E G T +
Sbjct: 14 KKDNIERQVELVNKAIDNKAKIIALDELSNTIY-FPFEQNPKYFSWAET-ERGETLQRFK 71
Query: 556 ELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNXIPR 702
E++ + + ++ I ERD S+ +NTA ++ D G +IGK+RK +P+
Sbjct: 72 EISKEREVSLIVPIFERD---SNFFYNTAFIL-DNGEIIGKYRKTHLPQ 116
>UniRef50_Q5KJU9 Cluster: Hydrolase, putative; n=1; Filobasidiella
neoformans|Rep: Hydrolase, putative - Cryptococcus
neoformans (Filobasidiella neoformans)
Length = 301
Score = 44.0 bits (99), Expect = 0.005
Identities = 33/93 (35%), Positives = 46/93 (49%), Gaps = 10/93 (10%)
Frame = +1
Query: 439 IICFQELWNXPFAFCT----REKQP-----WCEFAESDEDGPTTXFLRELA-IKYAMVIV 588
+I E+WN P+A + EK P W E +E G T LRE+A +I
Sbjct: 46 LIVLPEIWNSPYAVSSFREYSEKVPEVGSKWKSLKEGEE-GETIKALREMARSSGCWLIG 104
Query: 589 SSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
SI ERDEK +D ++NT V G ++ H+K
Sbjct: 105 GSIPERDEK-TDNIYNTCTVYDPEGTLVAVHQK 136
>UniRef50_Q1PXD4 Cluster: Similar to N-carbamoyl-D-amino acid
hydrolase; n=1; Candidatus Kuenenia stuttgartiensis|Rep:
Similar to N-carbamoyl-D-amino acid hydrolase -
Candidatus Kuenenia stuttgartiensis
Length = 277
Score = 42.7 bits (96), Expect = 0.012
Identities = 30/117 (25%), Positives = 58/117 (49%)
Frame = +1
Query: 337 SIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFA 516
SIA V+++ K + N + +++ A Q+G +I E F+F +E++ FA
Sbjct: 5 SIAAIQMCSVHDRNKNL-NTARVLMEKAVQKGARLIALPE----NFSFIGQEREN-ITFA 58
Query: 517 ESDEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
E E G FL++ ++K+++ I+ + + NT +V +G +IG + K
Sbjct: 59 EERETGEIVHFLKKFSMKHSVAIIGGSVPLRSSSKAKVTNTCLVFDQSGVIIGSYDK 115
>UniRef50_A0QPL8 Cluster: Hydrolase, carbon-nitrogen family protein;
n=6; Bacteria|Rep: Hydrolase, carbon-nitrogen family
protein - Mycobacterium smegmatis (strain ATCC 700084 /
mc(2)155)
Length = 330
Score = 42.3 bits (95), Expect = 0.016
Identities = 36/134 (26%), Positives = 60/134 (44%)
Frame = +1
Query: 298 RPPRIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFA 477
RPP ++VG+VQH RP + +++ ID A EG + E+ +
Sbjct: 20 RPP--LRVGLVQHRW-----RP---DAGELVKVLREGIDRAAGEGAKAVFLPEITLLRYP 69
Query: 478 FCTREKQPWCEFAESDEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISD 657
T + AE GPT E A + + +S+ E+ + +NTA+++S
Sbjct: 70 ADTPAGPNPGDVAEDLTGGPTFELAAEAARANGIFVHASLYEKAPAADGLGYNTAILVSP 129
Query: 658 TGNVIGKHRKNXIP 699
G ++G+ RK IP
Sbjct: 130 EGELVGRTRKMHIP 143
>UniRef50_A6CFF3 Cluster: Putative nitrilase; n=1; Planctomyces
maris DSM 8797|Rep: Putative nitrilase - Planctomyces
maris DSM 8797
Length = 343
Score = 41.5 bits (93), Expect = 0.028
Identities = 32/117 (27%), Positives = 46/117 (39%), Gaps = 8/117 (6%)
Frame = +1
Query: 361 PVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQP------WCEFAES 522
PV K A K +I A + G +I F E + F + P +CE A +
Sbjct: 15 PVFLNKDATVEKSCSLIREAARNGAQMIVFPETYIPAFPVWCALQAPIHNHDLFCELAAN 74
Query: 523 D--EDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
DGP + E A + M + E +WN +I D GN++ HRK
Sbjct: 75 SIKVDGPELAQIAETARECEMFVSMGFNEGTTVSDGCIWNANALIGDDGNILCHHRK 131
>UniRef50_A3ZLM3 Cluster: Putative nitrilase; n=1; Blastopirellula
marina DSM 3645|Rep: Putative nitrilase -
Blastopirellula marina DSM 3645
Length = 258
Score = 41.5 bits (93), Expect = 0.028
Identities = 30/107 (28%), Positives = 53/107 (49%), Gaps = 1/107 (0%)
Frame = +1
Query: 370 EQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXF 549
E K+ +++I A + G ++ EL+N + E AE+ GPT
Sbjct: 5 EDKELNLQTAERLIAQAAERGAQLVVLPELFNY-----LGRLENLVEHAETIS-GPTAVR 58
Query: 550 LRELAIKYAMVIVS-SILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
+R+ A+K+ + +V+ S ER E S + +NT+++ G IG +RK
Sbjct: 59 MRKAALKHQIYLVAGSFAERSETESRV-FNTSLIFDPLGKQIGVYRK 104
>UniRef50_Q6AMZ4 Cluster: Putative uncharacterized protein; n=1;
Desulfotalea psychrophila|Rep: Putative uncharacterized
protein - Desulfotalea psychrophila
Length = 258
Score = 41.1 bits (92), Expect = 0.037
Identities = 30/108 (27%), Positives = 53/108 (49%)
Frame = +1
Query: 364 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTT 543
V + K A + + I++ + ++I E+WN F + AE + GPT
Sbjct: 11 VEDDKAASIARARTEIELCRES--DLIILPEIWNTGFMNFAAYRS----LAE-ERKGPTL 63
Query: 544 XFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
+RE+A+K + I S EK D +N++ +IS G+++G +RK
Sbjct: 64 SMVREMAVKTSSFIHSGSFV--EKIEDKYYNSSYLISPDGDILGNYRK 109
>UniRef50_A6T2L9 Cluster: Nitrilase; n=1; Janthinobacterium sp.
Marseille|Rep: Nitrilase - Janthinobacterium sp. (strain
Marseille) (Minibacterium massiliensis)
Length = 355
Score = 40.7 bits (91), Expect = 0.048
Identities = 29/117 (24%), Positives = 54/117 (46%), Gaps = 8/117 (6%)
Frame = +1
Query: 361 PVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFA-FC-----TREKQPWCEFAES 522
P+ A +K +I A + G ++I F E + F +C + + + A S
Sbjct: 16 PIYFDTPATIDKACDLIAEAARNGASLIAFPEAFVSAFPIWCGVWAPVETHEFFFKLASS 75
Query: 523 --DEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
+ +GP LRE A ++ + + I E +W+T ++I D G+++ +HRK
Sbjct: 76 AIEINGPEVAQLREAARRHGVFVSMGINEGTPISMGCVWDTNILIGDDGSILNRHRK 132
>UniRef50_UPI0000E472D9 Cluster: PREDICTED: similar to
Ureidopropionase, beta, partial; n=1; Strongylocentrotus
purpuratus|Rep: PREDICTED: similar to Ureidopropionase,
beta, partial - Strongylocentrotus purpuratus
Length = 57
Score = 39.9 bits (89), Expect = 0.085
Identities = 15/29 (51%), Positives = 22/29 (75%)
Frame = +1
Query: 289 EQTRPPRIVKVGIVQHSIAVPTDRPVNEQ 375
EQ R PR+V++G++Q+ I +PT PV EQ
Sbjct: 29 EQLRSPRLVRIGLIQNQIVLPTTAPVKEQ 57
>UniRef50_Q5LLB2 Cluster: Nitrilase family protein; n=7;
Bacteria|Rep: Nitrilase family protein - Silicibacter
pomeroyi
Length = 344
Score = 39.9 bits (89), Expect = 0.085
Identities = 20/53 (37%), Positives = 27/53 (50%)
Frame = +1
Query: 529 DGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
DGP +R+ A + +V + ER L+NT + I G VIGKHRK
Sbjct: 83 DGPEIDVIRDAARAHGCHVVMGLNERSPVSLGALYNTLLFIGPDGEVIGKHRK 135
>UniRef50_Q8TPH5 Cluster: Carbon-nitrogen hydrolase; n=1;
Methanosarcina acetivorans|Rep: Carbon-nitrogen
hydrolase - Methanosarcina acetivorans
Length = 459
Score = 39.9 bits (89), Expect = 0.085
Identities = 21/50 (42%), Positives = 30/50 (60%), Gaps = 1/50 (2%)
Frame = +1
Query: 313 VKVGIVQHSIAVPTDRPVN-EQKKAIFNKVKKIIDVAGQEGVNIICFQEL 459
VKVG VQ + + P+ + K+A K+ K +D+A +E VNIIC EL
Sbjct: 194 VKVGTVQIAFELSESFPLEIKNKEATKEKIFKALDIANKENVNIICLPEL 243
>UniRef50_Q5V604 Cluster: Nitrilase; n=2; Halobacteriaceae|Rep:
Nitrilase - Haloarcula marismortui (Halobacterium
marismortui)
Length = 366
Score = 38.7 bits (86), Expect = 0.20
Identities = 29/126 (23%), Positives = 55/126 (43%), Gaps = 9/126 (7%)
Frame = +1
Query: 337 SIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELW--NXPFAFCTREKQPWCE 510
++A PV K+ +K + I+ AG++G +I+ F E + P+ + W +
Sbjct: 7 TLAAAQVEPVYHDKEGTLDKTCRYIEQAGRDGADIVVFPETYFPGYPYWRGSVSISRWTD 66
Query: 511 FAESDE------DGPTTXFLRELAIKYAMVIVSSILE-RDEKHSDILWNTAVVISDTGNV 669
+ D L E + + +V E D + S+ L+N+ +TG +
Sbjct: 67 LMVDLQKNSLHVDDEAIEILGEAVAEADLTLVLGTNEISDRQGSETLYNSLFYFDNTGEL 126
Query: 670 IGKHRK 687
+G+HRK
Sbjct: 127 MGRHRK 132
>UniRef50_Q6RWQ0 Cluster: Nitrilase; n=3; uncultured organism|Rep:
Nitrilase - uncultured organism
Length = 325
Score = 38.3 bits (85), Expect = 0.26
Identities = 32/116 (27%), Positives = 50/116 (43%), Gaps = 14/116 (12%)
Frame = +1
Query: 382 AIFNKVKKIIDVAGQEGVNIICFQE----------LWNXPFAFC--TREKQPWCEFAESD 525
A K ++I A + G N+I F E +W A R+K W +
Sbjct: 24 ATVEKACRLIGEAAENGANLIVFPEAFIPVYPNAAIWGRGLATFGGQRQKYVWTRLWNNS 83
Query: 526 ED--GPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
+ GP T L + A + +V + ER ++ L+NT + I G ++GKHRK
Sbjct: 84 VEIPGPATDRLAKAAHEARATVVMGLNER-AVDNNTLYNTLLFIGPDGRLLGKHRK 138
>UniRef50_Q2S196 Cluster: Hydrolase, carbon-nitrogen family; n=1;
Salinibacter ruber DSM 13855|Rep: Hydrolase,
carbon-nitrogen family - Salinibacter ruber (strain DSM
13855)
Length = 283
Score = 38.3 bits (85), Expect = 0.26
Identities = 30/124 (24%), Positives = 58/124 (46%), Gaps = 2/124 (1%)
Frame = +1
Query: 319 VGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPF--AFCTRE 492
+ +VQH+++ + V+ +A+ A G +++ F EL PF E
Sbjct: 3 IALVQHAVSPASPPRVDRGVRAV--------QAAADAGADLVVFPELSFTPFYPRVPVAE 54
Query: 493 KQPWCEFAESDEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVI 672
++ GPTT L E A +V+V +++ERD + + ++T+ V+ G ++
Sbjct: 55 RRRSARDLAEPVPGPTTEALAEAAADGGVVVVFNLMERDGERT---FDTSPVLDADGTLL 111
Query: 673 GKHR 684
G+ R
Sbjct: 112 GRTR 115
>UniRef50_Q1AWK1 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=4; Bacteria|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Rubrobacter xylanophilus (strain DSM
9941 / NBRC 16129)
Length = 276
Score = 37.9 bits (84), Expect = 0.34
Identities = 30/95 (31%), Positives = 49/95 (51%), Gaps = 1/95 (1%)
Frame = +1
Query: 406 IIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKYAMVI 585
+I A G ++ ELW+ C ++ + E AE GPTT FL LA + + +
Sbjct: 29 LIREAAAAGATLVALPELWS-----CHGLEEVYRENAEPIP-GPTTEFLGSLARELGIYL 82
Query: 586 V-SSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
+ SILER S+ L NT+ + + G+++ +RK
Sbjct: 83 LGGSILER-VSGSERLGNTSTLYAPDGSLVAVYRK 116
>UniRef50_Q44185 Cluster: N-carbamoyl-D-amino acid hydrolase; n=10;
Proteobacteria|Rep: N-carbamoyl-D-amino acid hydrolase -
Agrobacterium tumefaciens
Length = 304
Score = 37.9 bits (84), Expect = 0.34
Identities = 32/131 (24%), Positives = 60/131 (45%), Gaps = 8/131 (6%)
Frame = +1
Query: 331 QHSIAVPTDRPVN--EQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPF--AFCTREKQ 498
Q +AV P+ E ++ + ++ ++ A GVN I F EL F + ++
Sbjct: 4 QMILAVGQQGPIARAETREQVVGRLLDMLTNAASRGVNFIVFPELALTTFFPRWHFTDEA 63
Query: 499 PWCEFAESDEDGPTTXFL----RELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGN 666
F E++ GP L EL I + + ++E K +NT++++ +G
Sbjct: 64 ELDSFYETEMPGPVVRPLFETAAELGIGFNLGYAELVVEGGVKRR---FNTSILVDKSGK 120
Query: 667 VIGKHRKNXIP 699
++GK+RK +P
Sbjct: 121 IVGKYRKIHLP 131
>UniRef50_A1HQ26 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Thermosinus
carboxydivorans Nor1|Rep: Nitrilase/cyanide hydratase
and apolipoprotein N-acyltransferase - Thermosinus
carboxydivorans Nor1
Length = 258
Score = 37.1 bits (82), Expect = 0.60
Identities = 29/90 (32%), Positives = 44/90 (48%), Gaps = 1/90 (1%)
Frame = +1
Query: 421 GQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKY-AMVIVSSI 597
G +++ E+W +A RE W E D +G T + ++ KY A +I SI
Sbjct: 29 GAARADVVVLPEIWTTGYAL--REVDKWAE----DVEGLTISEMSNISRKYGAYIIAGSI 82
Query: 598 LERDEKHSDILWNTAVVISDTGNVIGKHRK 687
R K+ + +N AVVI GNV ++RK
Sbjct: 83 PLR--KNGKV-YNGAVVIGPDGNVAAEYRK 109
>UniRef50_A0TTW8 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=5;
Proteobacteria|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Burkholderia
cenocepacia MC0-3
Length = 299
Score = 37.1 bits (82), Expect = 0.60
Identities = 29/93 (31%), Positives = 45/93 (48%)
Frame = +1
Query: 409 IDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKYAMVIV 588
I+ A + G +I EL + + F R++ AE DGPT +A + + IV
Sbjct: 42 IETAARNGAALIVLPELASSGYVFEDRDEA--LALAELVPDGPTARAFEAIARRLNVHIV 99
Query: 589 SSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
S I ERD L+N+A + + G +G +RK
Sbjct: 100 SGIAERDGAR---LYNSA-LFAGPGGHLGVYRK 128
>UniRef50_A5GU42 Cluster: Nitrilase-related protein; n=1;
Synechococcus sp. RCC307|Rep: Nitrilase-related protein
- Synechococcus sp. (strain RCC307)
Length = 305
Score = 36.3 bits (80), Expect = 1.0
Identities = 30/128 (23%), Positives = 59/128 (46%), Gaps = 5/128 (3%)
Frame = +1
Query: 319 VGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAG-QEGVNIICFQELWNXPFAF--CTR 489
V +VQ ++ + VN Q+ + + +++ + AG ++ E+WN P+
Sbjct: 7 VALVQFQVS--PEPQVNRQQ--VCHWLEQAMTQAGTSSSPKLLMLPEVWNSPYQAERFAE 62
Query: 490 EKQPWCEFAESDEDGPTTXF--LRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTG 663
+P E DGP+ + + A+ + + +++ + I +NTA VIS G
Sbjct: 63 FAEPIPELGADLRDGPSDSLKVVADFAVSHRVSVIAGSIPECSSDGRI-FNTATVISPAG 121
Query: 664 NVIGKHRK 687
++ KHRK
Sbjct: 122 CLLAKHRK 129
>UniRef50_Q8W0T9 Cluster: Putative uncharacterized protein
SB35P03.20; n=1; Sorghum bicolor|Rep: Putative
uncharacterized protein SB35P03.20 - Sorghum bicolor
(Sorghum) (Sorghum vulgare)
Length = 580
Score = 36.3 bits (80), Expect = 1.0
Identities = 27/94 (28%), Positives = 45/94 (47%), Gaps = 5/94 (5%)
Frame = +1
Query: 451 QELWNXPFAFCTREKQPWCEFAESDEDG--PTTXFLRELAIKYAMVIVSSILERDEKHSD 624
+E+W+ C+ + +AE + G P+ L E+A + IV + EK S
Sbjct: 385 KEIWS-----CSYAMETLASYAEDIDGGESPSISMLSEVAAAKKITIVGGSIP--EKASG 437
Query: 625 ILWNTAVVISDTGNVIGKHRKNXIPRV---GDLT 717
++NT VI G ++ KHRK + + GD+T
Sbjct: 438 KMFNTCCVIGPDGKILAKHRKLHLFEIDIPGDIT 471
>UniRef50_Q6RWN7 Cluster: Nitrilase; n=21; root|Rep: Nitrilase -
uncultured organism
Length = 353
Score = 35.5 bits (78), Expect = 1.8
Identities = 19/47 (40%), Positives = 29/47 (61%), Gaps = 1/47 (2%)
Frame = +1
Query: 550 LRELAIKYAMVIVSSILERDEKHSDI-LWNTAVVISDTGNVIGKHRK 687
LR+ A + +V + ER+ + S L+NTA+VI G +IG+HRK
Sbjct: 89 LRDAARDGGVTVVIGVNERNTEASGASLYNTALVIGPLGQLIGRHRK 135
>UniRef50_Q9KE11 Cluster: BH1047 protein; n=1; Bacillus
halodurans|Rep: BH1047 protein - Bacillus halodurans
Length = 271
Score = 35.5 bits (78), Expect = 1.8
Identities = 40/136 (29%), Positives = 68/136 (50%), Gaps = 3/136 (2%)
Frame = +1
Query: 313 VKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKII-DVAGQEGV-NIICFQELWNXPFAFCT 486
+KV + Q I +P D NE+K VK+ I DV QE V +++ E+W +
Sbjct: 1 MKVALYQMDI-LPGDPRGNERK------VKEWIEDVMQQEDVPDLLVLPEMWTTAYTLDQ 53
Query: 487 REKQPWCEFAESDEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGN 666
E AE +E T FL+ELA ++ + IV+ + + EK L+N A+V G+
Sbjct: 54 LE-----HLAEGEERY-TELFLKELAREHNVNIVAGSIAKKEKGK--LYNRALVFDRRGH 105
Query: 667 VIGKHRK-NXIPRVGD 711
+ ++ K + +P + +
Sbjct: 106 TVYQYDKIHLVPMLSE 121
>UniRef50_Q4KB18 Cluster: Hydrolase, carbon-nitrogen family; n=2;
Bacteria|Rep: Hydrolase, carbon-nitrogen family -
Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Length = 325
Score = 35.5 bits (78), Expect = 1.8
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = +1
Query: 550 LRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
LRE A ++ +V + ER +H L+N+ V I G ++ HRK
Sbjct: 95 LREAARVNSVTVVMGMNERSRRHGGSLYNSLVTIGPEGTILNVHRK 140
>UniRef50_A4WA35 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=12; Bacteria|Rep:
Nitrilase/cyanide hydratase and apolipoprotein
N-acyltransferase - Enterobacter sp. 638
Length = 326
Score = 35.5 bits (78), Expect = 1.8
Identities = 30/102 (29%), Positives = 46/102 (45%), Gaps = 5/102 (4%)
Frame = +1
Query: 397 VKKIIDVAGQEGVNIICFQEL-----WNXPFAFCTREKQPWCEFAESDEDGPTTXFLREL 561
++K I+ A E VNI+ F E+ W+ P AE + P+ +R L
Sbjct: 28 IEKFIEQAALEQVNILVFPEMCITGYWHVPKLTAAEVSA----LAEPIAESPSLTLIRSL 83
Query: 562 AIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
AIK+ M+I ++ER + L+N V G + HRK
Sbjct: 84 AIKHQMLIGVGLIERAD--DGRLYNAYVACMPDG-TMHTHRK 122
>UniRef50_Q5MD29 Cluster: CtaJ; n=2; Cystobacteraceae|Rep: CtaJ -
Cystobacter fuscus
Length = 343
Score = 35.1 bits (77), Expect = 2.4
Identities = 28/100 (28%), Positives = 47/100 (47%)
Frame = +1
Query: 400 KKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKYAM 579
+ I A ++G ++ E + P + + + W A DGPT FL++ A ++ +
Sbjct: 34 RPFIQSAAEQGAQLLLLPEFY--PTGYL-QSPEVWR--AGETLDGPTVRFLKQQAAQWRV 88
Query: 580 VIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNXIP 699
+ +S LE D D +N V++S G V K RK P
Sbjct: 89 HLGTSFLEAD---GDDFYNAFVLVSPAGQV-HKVRKRRAP 124
>UniRef50_Q1AZG5 Cluster: Nitrilase; n=1; Rubrobacter xylanophilus
DSM 9941|Rep: Nitrilase - Rubrobacter xylanophilus
(strain DSM 9941 / NBRC 16129)
Length = 359
Score = 35.1 bits (77), Expect = 2.4
Identities = 28/121 (23%), Positives = 54/121 (44%), Gaps = 8/121 (6%)
Frame = +1
Query: 361 PVNEQKKAIFNKVKKIIDVAGQEGVNIICFQE-------LWNXPFAFCTREKQPWCEFAE 519
PV+ + A +K++ ++ A + G ++ F E +WN + F
Sbjct: 18 PVHLKPDATVDKLESLVAEAARGGAQLVVFSESFIPAFPVWNLVLPPVDQHDLFRRLFLN 77
Query: 520 SD-EDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNXI 696
S GP T L E+A ++ + + + ER L+NT ++ + TG ++ HR+ +
Sbjct: 78 SVLVPGPITRRLAEIAKRHDVYLSVGVTERTNISMGCLYNTNLLFAPTGELL-NHRRKLV 136
Query: 697 P 699
P
Sbjct: 137 P 137
>UniRef50_A1VWX6 Cluster: Nitrilase; n=2; Comamonadaceae|Rep:
Nitrilase - Polaromonas naphthalenivorans (strain CJ2)
Length = 341
Score = 35.1 bits (77), Expect = 2.4
Identities = 28/110 (25%), Positives = 47/110 (42%), Gaps = 8/110 (7%)
Frame = +1
Query: 382 AIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQP-----WCE---FAESDEDGP 537
A KV K++ A G +I+ F E++ + + K P W + F+ D GP
Sbjct: 23 ATMQKVGKLVREAASAGASIVVFPEVFVSGYPYWNWLKNPLDGSAWFQRLYFSAIDVPGP 82
Query: 538 TTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
L L+ + I + ER K ++NT ++ S +I + RK
Sbjct: 83 EVEELCRLSRDNNIHIAIGVNERGAKSVGTIYNTNLLFSPEKGLINRQRK 132
>UniRef50_Q75TH8 Cluster: Putative uncharacterized protein GSB07;
n=1; Geobacillus stearothermophilus|Rep: Putative
uncharacterized protein GSB07 - Bacillus
stearothermophilus (Geobacillus stearothermophilus)
Length = 273
Score = 34.7 bits (76), Expect = 3.2
Identities = 29/118 (24%), Positives = 53/118 (44%), Gaps = 2/118 (1%)
Frame = +1
Query: 340 IAVPTDRPVNEQKKAIFNKVKKIIDVAGQE--GVNIICFQELWNXPFAFCTREKQPWCEF 513
IA+ P + A K++ II ++ V ++ F EL+ + K+
Sbjct: 7 IALAQMMPADGDIGANLAKMETIIHECKRKFPNVRLLLFPELYTTGYVLSEMLKE----- 61
Query: 514 AESDEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
A DG T + +LA + + + +E+D H+ L+N+ ++I G IG +RK
Sbjct: 62 AAQTWDGSTFQHMSQLAQTFQLYLAYGYVEKD--HTGNLYNSLMLIDPNGQCIGNYRK 117
>UniRef50_A2BNC1 Cluster: Predicted amidohydrolase; n=1;
Hyperthermus butylicus DSM 5456|Rep: Predicted
amidohydrolase - Hyperthermus butylicus (strain DSM 5456
/ JCM 9403)
Length = 269
Score = 34.7 bits (76), Expect = 3.2
Identities = 17/53 (32%), Positives = 32/53 (60%)
Frame = +1
Query: 529 DGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
+GP F LA +Y++ +V+++ E+ K +NTA +I+ TG ++ +RK
Sbjct: 67 EGPWIGFFARLAREYSVHVVATLYEKS-KAGGKPYNTAALIAPTGELLAVYRK 118
>UniRef50_A2BKF1 Cluster: Predicted amidohydrolase; n=1;
Hyperthermus butylicus DSM 5456|Rep: Predicted
amidohydrolase - Hyperthermus butylicus (strain DSM 5456
/ JCM 9403)
Length = 272
Score = 34.7 bits (76), Expect = 3.2
Identities = 20/61 (32%), Positives = 32/61 (52%)
Frame = +1
Query: 514 AESDEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNX 693
AE+ +D P FL E++ +Y VIVS LER D +++ V++ V +RK
Sbjct: 60 AENPKDSPFIRFLEEISSEYTAVIVSGFLERS---GDCAYSSIVMVEPGKEVQVVYRKTV 116
Query: 694 I 696
+
Sbjct: 117 L 117
>UniRef50_Q9ZMC7 Cluster: Putative; n=6; Campylobacterales|Rep:
Putative - Helicobacter pylori J99 (Campylobacter pylori
J99)
Length = 294
Score = 34.3 bits (75), Expect = 4.2
Identities = 33/130 (25%), Positives = 60/130 (46%), Gaps = 3/130 (2%)
Frame = +1
Query: 307 RIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCT 486
RI+K ++Q +NE + N K+ A +G N+I EL++ +
Sbjct: 9 RILKTAVIQMQ---SKPYALNENLQLALNLAKE----AHNKGANLIVLPELFDSGYCVND 61
Query: 487 REKQPWCEFA--ESDEDGPTTXFLRELAIKYAMVIVSSILERD-EKHSDILWNTAVVISD 657
++ +F E E+ LR L+ +A + I+ EK++ L+++A +I
Sbjct: 62 KDADFGLDFKAIEHGEETLKNETLRALS-DFAKSSDTHIVACSIEKNNKKLYDSAYIIPP 120
Query: 658 TGNVIGKHRK 687
G ++GKHRK
Sbjct: 121 KGKIVGKHRK 130
>UniRef50_Q6N746 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=11;
Proteobacteria|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Rhodopseudomonas
palustris
Length = 579
Score = 34.3 bits (75), Expect = 4.2
Identities = 18/46 (39%), Positives = 28/46 (60%)
Frame = +1
Query: 532 GPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNV 669
GP T L LA + ++ +V + ERD DIL+N+AV+I+ G +
Sbjct: 349 GPATDRLAALASELSLYLVCGLAERD---GDILYNSAVLIAPDGTI 391
>UniRef50_Q84FR7 Cluster: D-N-carbamoylase; n=1; Arthrobacter
crystallopoietes|Rep: D-N-carbamoylase - Arthrobacter
crystallopoietes
Length = 315
Score = 34.3 bits (75), Expect = 4.2
Identities = 26/118 (22%), Positives = 55/118 (46%), Gaps = 3/118 (2%)
Frame = +1
Query: 367 NEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCTR-EKQPWCEFAESD--EDGP 537
+E + + ++ +++ A +G ++ F EL F T E+ + E+ + D
Sbjct: 18 SESRPEVVARLIALLEEAASQGAELVVFPELTLTTFFPRTWFEEGDFEEYFDKSMPNDDV 77
Query: 538 TTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNXIPRVGD 711
F R + + + L DEK +NT+++++ G+++GK+RK +P D
Sbjct: 78 APLFERAKDLGVGFYLGYAELTSDEKR----YNTSILVNKHGDIVGKYRKMHLPGHAD 131
>UniRef50_A7DA57 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2; Methylobacterium
extorquens PA1|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Methylobacterium
extorquens PA1
Length = 369
Score = 34.3 bits (75), Expect = 4.2
Identities = 16/53 (30%), Positives = 25/53 (47%)
Frame = +1
Query: 529 DGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
DGP +R A ++ +++ E E LWN V+I G ++ HRK
Sbjct: 81 DGPEIGAVRAAARRHGVLVSLGFSESTEASVGCLWNANVLIGRDGAILNHHRK 133
>UniRef50_Q2GWJ9 Cluster: Putative uncharacterized protein; n=1;
Chaetomium globosum|Rep: Putative uncharacterized
protein - Chaetomium globosum (Soil fungus)
Length = 1646
Score = 34.3 bits (75), Expect = 4.2
Identities = 19/46 (41%), Positives = 23/46 (50%)
Frame = +1
Query: 349 PTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNXPFAFCT 486
PT RP E+ K F ++KI A Q G+ I E WN FA T
Sbjct: 20 PTYRPTEEEWKEPFEYIRKISPEARQYGICKIIPPESWNPDFAIDT 65
>UniRef50_Q8TPH6 Cluster: Putative uncharacterized protein; n=1;
Methanosarcina acetivorans|Rep: Putative uncharacterized
protein - Methanosarcina acetivorans
Length = 1078
Score = 34.3 bits (75), Expect = 4.2
Identities = 18/51 (35%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
Frame = +1
Query: 310 IVKVGIVQHSIAVPTDRPVNE-QKKAIFNKVKKIIDVAGQEGVNIICFQEL 459
IV++G Q + + P K+A +KV K++D+A +E V+I+C EL
Sbjct: 785 IVRIGTAQINFELSESFPPEIIDKEATRDKVFKVLDIATKEKVDIVCLSEL 835
>UniRef50_A0L7H1 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=2;
Proteobacteria|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Magnetococcus sp.
(strain MC-1)
Length = 275
Score = 33.9 bits (74), Expect = 5.6
Identities = 18/98 (18%), Positives = 47/98 (47%)
Frame = +1
Query: 394 KVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKY 573
+ +++++ A G ++ E F+F +++ E + GP+ ++ A ++
Sbjct: 26 RAEQLLEEAATAGAKLLVLPE----NFSFFGADEKEKLAHQEDPQHGPSLRMVQAFAQRH 81
Query: 574 AMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 687
+V+ + D S + N++ V++D G V+ ++ K
Sbjct: 82 GAWVVAGSIPTDVGESQRVANSSFVVNDQGQVVARYDK 119
>UniRef50_A5D6C3 Cluster: Putative uncharacterized protein; n=1;
Pelotomaculum thermopropionicum SI|Rep: Putative
uncharacterized protein - Pelotomaculum
thermopropionicum SI
Length = 256
Score = 33.5 bits (73), Expect = 7.4
Identities = 21/57 (36%), Positives = 28/57 (49%), Gaps = 2/57 (3%)
Frame = +1
Query: 523 DEDGPTTXFLRELAIKYAMVIVSSILERDEKHSD--ILWNTAVVISDTGNVIGKHRK 687
D G T L E A +Y + I LERD+ D +NT +I G +I K+RK
Sbjct: 85 DIPGEETERLAEKAKEYQIYIAGCALERDKDWIDDGYFFNTHFIIGPDGKIIHKYRK 141
>UniRef50_A1HPP3 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Thermosinus
carboxydivorans Nor1|Rep: Nitrilase/cyanide hydratase
and apolipoprotein N-acyltransferase - Thermosinus
carboxydivorans Nor1
Length = 259
Score = 33.5 bits (73), Expect = 7.4
Identities = 29/100 (29%), Positives = 43/100 (43%), Gaps = 2/100 (2%)
Frame = +1
Query: 394 KVKKIIDVAGQEGVNIICFQELWNXPFAFCTREKQPWCEFAESDEDGPTTXFLRELAIKY 573
K +++ + G + ELW + + E D GPT L++ A
Sbjct: 21 KALAMLEQGAKAGAKLFVLPELWTTGYVL-----DQLLKIGEPD-GGPTVKMLQQFAKDN 74
Query: 574 AMVIVS-SILE-RDEKHSDILWNTAVVISDTGNVIGKHRK 687
+ IV SI E RD K ++NT VI G V+GK+ K
Sbjct: 75 GVEIVGGSIAEIRDGK----VYNTIYVIDSAGEVVGKYSK 110
>UniRef50_Q6RWQ5 Cluster: Nitrilase; n=1; uncultured organism|Rep:
Nitrilase - uncultured organism
Length = 298
Score = 33.1 bits (72), Expect = 9.7
Identities = 18/65 (27%), Positives = 31/65 (47%)
Frame = +1
Query: 523 DEDGPTTXFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNXIPR 702
D GP L + A + + + ERD + LWNT + + G++ +HRK +P
Sbjct: 79 DVGGPLARELGDAARRADAWVAIGVNERDARRPGTLWNTLLWFAPDGSLARRHRK-LVPT 137
Query: 703 VGDLT 717
+ + T
Sbjct: 138 MHERT 142
>UniRef50_A6FX13 Cluster: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase; n=1; Plesiocystis
pacifica SIR-1|Rep: Nitrilase/cyanide hydratase and
apolipoprotein N-acyltransferase - Plesiocystis pacifica
SIR-1
Length = 347
Score = 33.1 bits (72), Expect = 9.7
Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Frame = +1
Query: 529 DGPTTXFLRELAIKYAMVIVSSILERD-EKHSDILWNTAVVISDTGNVIGKHRK 687
DGP + E + + + +V ++E E+HS + + TAV I ++G HRK
Sbjct: 69 DGPQLRAIAERSRRRGVAVVLGVVEASPERHSSV-YCTAVTIDPARGIVGAHRK 121
>UniRef50_A0QWL8 Cluster: Carbon-nitrogen hydrolase family protein;
n=6; Bacteria|Rep: Carbon-nitrogen hydrolase family
protein - Mycobacterium smegmatis (strain ATCC 700084 /
mc(2)155)
Length = 299
Score = 33.1 bits (72), Expect = 9.7
Identities = 15/46 (32%), Positives = 24/46 (52%)
Frame = +2
Query: 725 YYMXGTXGHPVFATRYGXIAVTICFGRXHVLXWMMFGQNGAEIVFI 862
++ G G+PVF TR G I + +C+ + Q GA+I+ I
Sbjct: 135 FFSPGDLGYPVFHTRIGRIGLLVCWDIWFPETARIVAQQGADIICI 180
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 748,215,691
Number of Sequences: 1657284
Number of extensions: 13956117
Number of successful extensions: 37718
Number of sequences better than 10.0: 73
Number of HSP's better than 10.0 without gapping: 36532
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 37696
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 79932179145
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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