BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP08_F_O03
(854 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 41 2e-04
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 30 0.48
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 30 0.48
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 3.4
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 27 4.5
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 27 4.5
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 27 4.5
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 27 4.5
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 5.9
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 26 5.9
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 41.1 bits (92), Expect = 2e-04
Identities = 21/56 (37%), Positives = 21/56 (37%)
Frame = +2
Query: 593 PPPPPPXXXGXGGGPXPXXXXXXXFXFXXXXGGGXXPXPPXXKXXXXXPPPPPPPP 760
PPPPPP P P GG P PP PPPPPPPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPA----PIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 34.7 bits (76), Expect = 0.017
Identities = 13/25 (52%), Positives = 13/25 (52%)
Frame = +1
Query: 688 GGXXXPPXPXKXXXXXXPPPPPPPP 762
GG PP P PPPPPPPP
Sbjct: 759 GGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 33.9 bits (74), Expect = 0.030
Identities = 14/34 (41%), Positives = 14/34 (41%)
Frame = +2
Query: 542 PXXXXPPPPXXXFXXXXPPPPPPXXXGXGGGPXP 643
P PPP PPPPPP G G P P
Sbjct: 746 PAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP 779
Score = 31.5 bits (68), Expect = 0.16
Identities = 12/25 (48%), Positives = 12/25 (48%)
Frame = +2
Query: 557 PPPPXXXFXXXXPPPPPPXXXGXGG 631
PPPP PPPPPP GG
Sbjct: 765 PPPPGVAGAGPPPPPPPPPAVSAGG 789
Score = 30.7 bits (66), Expect = 0.28
Identities = 12/26 (46%), Positives = 12/26 (46%)
Frame = +2
Query: 557 PPPPXXXFXXXXPPPPPPXXXGXGGG 634
PPPP PPPPPP GG
Sbjct: 764 PPPPPGVAGAGPPPPPPPPPAVSAGG 789
Score = 29.9 bits (64), Expect = 0.48
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = +2
Query: 557 PPPPXXXFXXXXPPPPPPXXXGXGGG 634
PPPP PPPPPP G
Sbjct: 763 PPPPPPGVAGAGPPPPPPPPPAVSAG 788
Score = 28.7 bits (61), Expect = 1.1
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +1
Query: 706 PXPXKXXXXXXPPPPPPPP 762
P P PPPPPPPP
Sbjct: 750 PVPPPAPIMGGPPPPPPPP 768
Score = 27.1 bits (57), Expect = 3.4
Identities = 11/29 (37%), Positives = 11/29 (37%)
Frame = +2
Query: 542 PXXXXPPPPXXXFXXXXPPPPPPXXXGXG 628
P PPP PPPPPP G
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPPAVSAGG 789
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 29.9 bits (64), Expect = 0.48
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = +2
Query: 542 PXXXXPPPPXXXFXXXXPPPPPP 610
P PPPP F PPPPP
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPP 28
Score = 29.5 bits (63), Expect = 0.64
Identities = 11/24 (45%), Positives = 11/24 (45%)
Frame = +1
Query: 688 GGXXXPPXPXKXXXXXXPPPPPPP 759
G PP P PPPPPPP
Sbjct: 7 GNPPPPPPPPGFEPPSQPPPPPPP 30
Score = 29.5 bits (63), Expect = 0.64
Identities = 11/24 (45%), Positives = 11/24 (45%)
Frame = +1
Query: 691 GXXXPPXPXKXXXXXXPPPPPPPP 762
G PP P PPPPPPP
Sbjct: 7 GNPPPPPPPPGFEPPSQPPPPPPP 30
Score = 28.7 bits (61), Expect = 1.1
Identities = 11/24 (45%), Positives = 11/24 (45%)
Frame = +2
Query: 686 GGGXXPXPPXXKXXXXXPPPPPPP 757
G P PP PPPPPPP
Sbjct: 7 GNPPPPPPPPGFEPPSQPPPPPPP 30
Score = 27.9 bits (59), Expect = 1.9
Identities = 10/18 (55%), Positives = 10/18 (55%)
Frame = +2
Query: 557 PPPPXXXFXXXXPPPPPP 610
PPPP PPPPPP
Sbjct: 13 PPPPGFEPPSQPPPPPPP 30
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.9 bits (64), Expect = 0.48
Identities = 16/56 (28%), Positives = 16/56 (28%)
Frame = +2
Query: 593 PPPPPPXXXGXGGGPXPXXXXXXXFXFXXXXGGGXXPXPPXXKXXXXXPPPPPPPP 760
PPPPPP G P P PP PP PPP
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPP 393
Score = 28.3 bits (60), Expect = 1.5
Identities = 18/58 (31%), Positives = 19/58 (32%), Gaps = 4/58 (6%)
Frame = +2
Query: 593 PPPPPPXXXGXGGGPXPXXXXXXXF----XFXXXXGGGXXPXPPXXKXXXXXPPPPPP 754
PPPPPP G P G P PP + PPPPPP
Sbjct: 312 PPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGR---SAPPPPPP 366
Score = 24.6 bits (51), Expect(2) = 2.7
Identities = 9/19 (47%), Positives = 9/19 (47%)
Frame = +1
Query: 703 PPXPXKXXXXXXPPPPPPP 759
PP PPPPPPP
Sbjct: 325 PPIGNGSSNSSLPPPPPPP 343
Score = 23.8 bits (49), Expect(2) = 0.74
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = +1
Query: 739 PPPPPPP 759
PPPPPPP
Sbjct: 311 PPPPPPP 317
Score = 23.8 bits (49), Expect(2) = 0.74
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = +1
Query: 742 PPPPPPP 762
PPPPPPP
Sbjct: 337 PPPPPPP 343
Score = 21.0 bits (42), Expect(2) = 2.7
Identities = 6/6 (100%), Positives = 6/6 (100%)
Frame = +1
Query: 745 PPPPPP 762
PPPPPP
Sbjct: 361 PPPPPP 366
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 27.1 bits (57), Expect = 3.4
Identities = 14/56 (25%), Positives = 16/56 (28%)
Frame = +2
Query: 593 PPPPPPXXXGXGGGPXPXXXXXXXFXFXXXXGGGXXPXPPXXKXXXXXPPPPPPPP 760
PPPPPP + P + PPPPPPP
Sbjct: 189 PPPPPPPPPAVEDQAADANEPDDYYSSGRAVSPEIPPTYTPKQADPLPAPPPPPPP 244
Score = 26.6 bits (56), Expect = 4.5
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 739 PPPPPPPP 762
PPPPPPPP
Sbjct: 189 PPPPPPPP 196
Score = 26.6 bits (56), Expect = 4.5
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 739 PPPPPPPP 762
PPPPPPPP
Sbjct: 190 PPPPPPPP 197
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 26.6 bits (56), Expect = 4.5
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +2
Query: 737 PPPPPPPP 760
PPPPPPPP
Sbjct: 306 PPPPPPPP 313
Score = 26.6 bits (56), Expect = 4.5
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 739 PPPPPPPP 762
PPPPPPPP
Sbjct: 306 PPPPPPPP 313
Score = 26.6 bits (56), Expect = 4.5
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +3
Query: 738 PPPPPPPP 761
PPPPPPPP
Sbjct: 307 PPPPPPPP 314
Score = 26.6 bits (56), Expect = 4.5
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 739 PPPPPPPP 762
PPPPPPPP
Sbjct: 307 PPPPPPPP 314
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 26.6 bits (56), Expect = 4.5
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +3
Query: 738 PPPPPPPP 761
PPPPPPPP
Sbjct: 1095 PPPPPPPP 1102
Score = 26.6 bits (56), Expect = 4.5
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 739 PPPPPPPP 762
PPPPPPPP
Sbjct: 1095 PPPPPPPP 1102
>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 26.6 bits (56), Expect = 4.5
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +3
Query: 738 PPPPPPPP 761
PPPPPPPP
Sbjct: 370 PPPPPPPP 377
Score = 26.6 bits (56), Expect = 4.5
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 739 PPPPPPPP 762
PPPPPPPP
Sbjct: 370 PPPPPPPP 377
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 26.6 bits (56), Expect = 4.5
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +2
Query: 737 PPPPPPPP 760
PPPPPPPP
Sbjct: 945 PPPPPPPP 952
Score = 26.6 bits (56), Expect = 4.5
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 739 PPPPPPPP 762
PPPPPPPP
Sbjct: 945 PPPPPPPP 952
Score = 26.6 bits (56), Expect = 4.5
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +3
Query: 738 PPPPPPPP 761
PPPPPPPP
Sbjct: 946 PPPPPPPP 953
Score = 26.6 bits (56), Expect = 4.5
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 739 PPPPPPPP 762
PPPPPPPP
Sbjct: 946 PPPPPPPP 953
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.2 bits (55), Expect = 5.9
Identities = 11/34 (32%), Positives = 11/34 (32%)
Frame = +2
Query: 542 PXXXXPPPPXXXFXXXXPPPPPPXXXGXGGGPXP 643
P P PP PP PP G P P
Sbjct: 1700 PQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPP 1733
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 26.2 bits (55), Expect = 5.9
Identities = 12/27 (44%), Positives = 12/27 (44%)
Frame = -3
Query: 636 GPPPXPXXXGGGGGGXXXKXKXXGGGG 556
GPPP P GG GG G GG
Sbjct: 199 GPPPGPGGFGGFGGFGGEGHHHGGHGG 225
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,960,920
Number of Sequences: 5004
Number of extensions: 29800
Number of successful extensions: 460
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 28
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 424464280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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