Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP08_F_N13
(861 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 229 4e-61
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 119 5e-28
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 106 5e-24
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 81 3e-16
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 59 9e-10
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 55 1e-08
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 47 4e-06
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 38 0.002
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 4.5
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 26 6.0
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 7.9
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 7.9
SPCC1020.12c ||SPCC14G10.06|xap-5-like protein|Schizosaccharomyc... 26 7.9
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 229 bits (560), Expect = 4e-61
Identities = 107/136 (78%), Positives = 112/136 (82%)
Frame = +1
Query: 148 NGSGMCKAGFAGXXAPRAVFPSIVGRPRHQGVMVGMGQXDSYVGXEAQSKXGILTLKYPI 327
NGSGMCKAGFAG APRAVFPSIVGRPRH G+MVGMGQ DSYVG EAQSK GILTLKYPI
Sbjct: 12 NGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPI 71
Query: 328 EHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKGQQGRR*PRSCLRPSTPP 507
EHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK + + + P
Sbjct: 72 EHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKM-TQIIFETFNAP 130
Query: 508 XMYVAIQAVLSLYASG 555
YVAIQAVLSLYASG
Sbjct: 131 AFYVAIQAVLSLYASG 146
Score = 106 bits (255), Expect = 3e-24
Identities = 67/125 (53%), Positives = 79/125 (63%), Gaps = 1/125 (0%)
Frame = +3
Query: 465 EKMTQIMFETFNSPX-HVRRHPGRALAVRLRVVPPVSCSTPEMVSPTPYPSTKVTLCPTP 641
EKMTQI+FETFN+P +V +L R V + + V+ T P + P
Sbjct: 117 EKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIV-LDSGDGVTHT-VPIYEGYALPHA 174
Query: 642 SSGLDLAGRDLTDYLMKILTERGYSFTTTAERXIVRDIKEKLXYVAXXFXQEMXTXAAXT 821
LDLAGRDLTDYLMKIL ERGY+F+TTAER IVRDIKEKL YVA F QE+ T A +
Sbjct: 175 IMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSS 234
Query: 822 SLXKS 836
SL KS
Sbjct: 235 SLEKS 239
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 119 bits (287), Expect = 5e-28
Identities = 62/137 (45%), Positives = 86/137 (62%), Gaps = 2/137 (1%)
Frame = +1
Query: 148 NGSGMCKAGFAGXXAPRAVFPSIVGRPRHQGVMVGMGQXDSYVGXEAQSKXGILTLKYPI 327
NGSG KAGFAG P+ +FP+ VGR +H+ VM Q D +VG EAQ+ G+L ++ PI
Sbjct: 16 NGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKIQRPI 75
Query: 328 EHGIITNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPL-NPKGQQGRR*PRSCLRPST 501
E GII NW DME+IW + + + +L PEEHP+LLTE PL N + ++ +
Sbjct: 76 ERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKE--KIAEYFYETLN 133
Query: 502 PPXMYVAIQAVLSLYAS 552
P + ++Q VL+LYAS
Sbjct: 134 VPALSFSLQPVLALYAS 150
Score = 54.4 bits (125), Expect = 2e-08
Identities = 38/114 (33%), Positives = 53/114 (46%), Gaps = 2/114 (1%)
Frame = +3
Query: 465 EKMTQIMFETFNSPX-HVRRHPGRALAVRLRVVPPV-SCSTPEMVSPTPYPSTKVTLCPT 638
EK+ + +ET N P P AL R V C S Y + P+
Sbjct: 122 EKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSI---PS 178
Query: 639 PSSGLDLAGRDLTDYLMKILTERGYSFTTTAERXIVRDIKEKLXYVAXXFXQEM 800
++ GRD+TDYL L + G+ ++AE+ IVR+IKEK YVA F E+
Sbjct: 179 AIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEI 232
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 106 bits (254), Expect = 5e-24
Identities = 57/138 (41%), Positives = 83/138 (60%), Gaps = 2/138 (1%)
Frame = +1
Query: 148 NGSGMCKAGFAGXXAPRAVFPSIVGRP--RHQGVMVGMGQXDSYVGXEAQSKXGILTLKY 321
NG+G K G+A PR FPSIVGRP R + + D VG EA++ +L +KY
Sbjct: 10 NGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRSLLQVKY 69
Query: 322 PIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKGQQGRR*PRSCLRPST 501
P+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP + + R
Sbjct: 70 PMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERYGF 129
Query: 502 PPXMYVAIQAVLSLYASG 555
+YVAIQAVLSLYA G
Sbjct: 130 -GGVYVAIQAVLSLYAQG 146
Score = 52.4 bits (120), Expect = 8e-08
Identities = 23/43 (53%), Positives = 32/43 (74%)
Frame = +3
Query: 651 LDLAGRDLTDYLMKILTERGYSFTTTAERXIVRDIKEKLXYVA 779
LD+AGRD T YL+ +L +GY+F TA+ VR++KEKL YV+
Sbjct: 178 LDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVS 220
Score = 41.1 bits (92), Expect = 2e-04
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = +2
Query: 566 GIVLDSGDGVSHTVPIYEGYALPHAILR 649
G+V+DSGDGV+H VP+YE L H + R
Sbjct: 150 GVVVDSGDGVTHIVPVYESVVLNHLVGR 177
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 80.6 bits (190), Expect = 3e-16
Identities = 54/159 (33%), Positives = 72/159 (45%), Gaps = 24/159 (15%)
Frame = +1
Query: 148 NGSGMCKAGFAGXXAPRAVFPSIVGRPRHQGVMVGMG----------------------- 258
NG+G K G+AG AP VFP+++ R G G
Sbjct: 12 NGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSKRATE 70
Query: 259 QXDSYVGXEAQSKXGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 435
D ++G +A K +L YPI HG I NWD ME+ W + + LR PE+H LLTE
Sbjct: 71 DLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTE 130
Query: 436 APLNPKGQQGRR*PRSCLRPSTPPXMYVAIQAVLSLYAS 552
PLNP + +Y+A+QAVL+L AS
Sbjct: 131 PPLNPP-ENRENTAEIMFESFNCAGLYIAVQAVLALAAS 168
Score = 37.5 bits (83), Expect = 0.002
Identities = 14/26 (53%), Positives = 20/26 (76%)
Frame = +2
Query: 566 GIVLDSGDGVSHTVPIYEGYALPHAI 643
G V+DSGDGV+H +P+ EGY + +I
Sbjct: 181 GTVVDSGDGVTHIIPVAEGYVIGSSI 206
Score = 27.1 bits (57), Expect = 3.4
Identities = 19/51 (37%), Positives = 27/51 (52%), Gaps = 2/51 (3%)
Frame = +3
Query: 651 LDLAGRDLTDYLMKILTERGY--SFTTTAERXIVRDIKEKLXYVAXXFXQE 797
+ LAGRD+T ++ +L +R S TAER IKE+ YV +E
Sbjct: 209 MPLAGRDVTYFVQSLLRDRNEPDSSLKTAER-----IKEECCYVCPDIVKE 254
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 58.8 bits (136), Expect = 9e-10
Identities = 33/102 (32%), Positives = 56/102 (54%), Gaps = 1/102 (0%)
Frame = +1
Query: 148 NGSGMCKAGFAGXXAPRAVFPSIVGRPRHQGVMVGMGQXDSYVGXEAQSKXGILTL-KYP 324
NGS +AG+ G P+ VF ++V R R + + + + VG + + G ++ + P
Sbjct: 31 NGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK----LSRTSTLVGNDTLIEVGSRSIARSP 86
Query: 325 IEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNP 450
E +I+NWD ME++ +TF +L + EHP+ +TE NP
Sbjct: 87 FERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANP 127
Score = 26.2 bits (55), Expect = 6.0
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = +2
Query: 566 GIVLDSGDGVSHTVPIYEGYALPHAILRSGLGWSR 670
GIVL+ G+ SH +P+ G + R G S+
Sbjct: 167 GIVLNLGNAASHVIPVLNGERILSEAKRISWGGSQ 201
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 54.8 bits (126), Expect = 1e-08
Identities = 38/135 (28%), Positives = 61/135 (45%)
Frame = +1
Query: 151 GSGMCKAGFAGXXAPRAVFPSIVGRPRHQGVMVGMGQXDSYVGXEAQSKXGILTLKYPIE 330
GS + GF+G P+ V PS G + G + Y+ +S G + +K I
Sbjct: 19 GSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNPG-MEIKNAIR 70
Query: 331 HGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKGQQGRR*PRSCLRPSTPPX 510
+G + NWD +W + +L+ P EHP+L+TE NP + + + P
Sbjct: 71 NGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKT-LETMFESLRCPA 129
Query: 511 MYVAIQAVLSLYASG 555
Y+A Q + +ASG
Sbjct: 130 TYLAKQETCAAFASG 144
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 46.8 bits (106), Expect = 4e-06
Identities = 35/136 (25%), Positives = 61/136 (44%), Gaps = 2/136 (1%)
Frame = +1
Query: 151 GSGMCKAGFAGXXAPRAVFPSIVGRPRHQGVMVGMGQXDSYVGXEAQSKXGI--LTLKYP 324
GS + G+AG +P + PS + GV + + YV E Q I + +K
Sbjct: 18 GSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHAPIPGMEVKNG 71
Query: 325 IEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKGQQGRR*PRSCLRPSTP 504
+GII +W+ W +L+V P E+ +++TE NP+ + ++ +
Sbjct: 72 KSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVR-QQIMEAAFEQLHV 130
Query: 505 PXMYVAIQAVLSLYAS 552
P Y+ QAV +A+
Sbjct: 131 PAFYLTKQAVCVAFAN 146
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 37.9 bits (84), Expect = 0.002
Identities = 18/50 (36%), Positives = 32/50 (64%)
Frame = +3
Query: 651 LDLAGRDLTDYLMKILTERGYSFTTTAERXIVRDIKEKLXYVAXXFXQEM 800
+D+ G+ LT+YL ++++ R Y+ E IV +IKE + YV+ F ++M
Sbjct: 186 VDVGGKLLTNYLKEVISYRKYNMME--ETYIVNEIKESVCYVSQNFKEDM 233
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 26.6 bits (56), Expect = 4.5
Identities = 11/29 (37%), Positives = 14/29 (48%)
Frame = +3
Query: 558 VPPVSCSTPEMVSPTPYPSTKVTLCPTPS 644
VP S P P P PS+++ P PS
Sbjct: 1052 VPKSSSGAPSAPPPVPAPSSEIPSIPAPS 1080
Score = 25.8 bits (54), Expect = 7.9
Identities = 13/31 (41%), Positives = 14/31 (45%)
Frame = +3
Query: 561 PPVSCSTPEMVSPTPYPSTKVTLCPTPSSGL 653
PPV P P P PS P PSSG+
Sbjct: 1150 PPVPA--PSGAPPVPKPSVAAPPVPAPSSGI 1178
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 26.2 bits (55), Expect = 6.0
Identities = 20/85 (23%), Positives = 34/85 (40%), Gaps = 4/85 (4%)
Frame = +1
Query: 322 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKGQQGRR*PRSCL 489
PI+ G + +W+ ++ W H Y+ L P + +PV L P +
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLV-IPTYWSLYDRELATQFFF 170
Query: 490 RPSTPPXMYVAIQAVLSLYASGSYH 564
P +A + ++ LYA G H
Sbjct: 171 EECQVPGFTIAYEPLMGLYAIGILH 195
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.8 bits (54), Expect = 7.9
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +3
Query: 558 VPPVSCSTPEMVSPTPYPSTKVTLCPTPSS 647
V P S + P+M +PTP P +++ P PS+
Sbjct: 1692 VRPQSAAPPQMSAPTP-PPPPMSVPPPPSA 1720
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 25.8 bits (54), Expect = 7.9
Identities = 14/35 (40%), Positives = 16/35 (45%)
Frame = -1
Query: 345 GDDTVLDGVLQSEDTXLALGLXTHVGVXLAHTDHH 241
GDD+VLD V +D L L AH D H
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFH 710
>SPCC1020.12c ||SPCC14G10.06|xap-5-like protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 288
Score = 25.8 bits (54), Expect = 7.9
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +3
Query: 567 VSCSTPEMVSPTPYPS 614
+SCS+PEMV+ T PS
Sbjct: 222 LSCSSPEMVATTQLPS 237
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,784,243
Number of Sequences: 5004
Number of extensions: 50850
Number of successful extensions: 183
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 428468660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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