BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP08_F_M16
(1034 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 34 0.029
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 30 0.46
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 30 0.46
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 30 0.61
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 0.81
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 29 1.1
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 29 1.4
SPBP8B7.26 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 28 2.5
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 3.3
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 4.3
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 5.7
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 34.3 bits (75), Expect = 0.029
Identities = 20/59 (33%), Positives = 22/59 (37%), Gaps = 4/59 (6%)
Frame = -1
Query: 599 PPRXPPX-PXAGXXAXAXXXXGXPXXPAXGPXXPRXPA---AAPPRXXXXQXPPPPPXP 435
PP PP P + + P P P P PA AAPP PPP P P
Sbjct: 424 PPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAP 482
Score = 28.7 bits (61), Expect = 1.4
Identities = 13/36 (36%), Positives = 16/36 (44%), Gaps = 1/36 (2%)
Frame = -1
Query: 539 GXPXXPAXGPXXPRXPAAAPP-RXXXXQXPPPPPXP 435
G P + P P P++ PP R P PPP P
Sbjct: 222 GTPTSTSAPPIPPSIPSSRPPERVPSLSAPAPPPIP 257
Score = 27.9 bits (59), Expect = 2.5
Identities = 22/69 (31%), Positives = 25/69 (36%), Gaps = 1/69 (1%)
Frame = -1
Query: 602 APPRXPPXPXAGXXAX-AXXXXGXPXXPAXGPXXPRXPAAAPPRXXXXQXPPPPPXPXXN 426
+PP PP P A A P P P R AAA + PPPPP N
Sbjct: 266 SPPNSPPRPIAPVSMNPAINSTSKPPLP---PPSSRVSAAALAANKK-RPPPPPPPSRRN 321
Query: 425 PXRRXXGXG 399
+ G G
Sbjct: 322 RGKPPIGNG 330
Score = 26.2 bits (55), Expect = 7.6
Identities = 16/57 (28%), Positives = 16/57 (28%), Gaps = 3/57 (5%)
Frame = -1
Query: 587 PPXPXAGXXAXAXXXXGXPXXPAXGPXXPRXPAAAPP---RXXXXQXPPPPPXPXXN 426
PP P A P P R PP PPPPP P N
Sbjct: 290 PPLPPPSSRVSAAALAANKKRPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSN 346
Score = 25.8 bits (54), Expect = 10.0
Identities = 19/61 (31%), Positives = 19/61 (31%), Gaps = 2/61 (3%)
Frame = -1
Query: 599 PPRXPPX--PXAGXXAXAXXXXGXPXXPAXGPXXPRXPAAAPPRXXXXQXPPPPPXPXXN 426
PP PP P A G P P P P P A P PP PP
Sbjct: 420 PPSLPPSAPPSLPPSAPPSLPMGAPAAP---PLPPSAPIAPPLPAGMPAAPPLPPAAPAP 476
Query: 425 P 423
P
Sbjct: 477 P 477
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 30.3 bits (65), Expect = 0.46
Identities = 25/62 (40%), Positives = 25/62 (40%)
Frame = +1
Query: 418 RXGFXXGXGGGGGXXXXXXRGGAAAGXRGXXGPXAGXXGXPXXXXAXAXXPAXGXGGXRG 597
R GF G GG GG RGGA G RG G G G A G G RG
Sbjct: 18 RGGFNGGRGGFGGG-----RGGARGGGRG--GARGGRGGR-----GGARGGRGGSSGGRG 65
Query: 598 GA 603
GA
Sbjct: 66 GA 67
Score = 30.3 bits (65), Expect = 0.46
Identities = 21/60 (35%), Positives = 23/60 (38%)
Frame = +1
Query: 424 GFXXGXGGGGGXXXXXXRGGAAAGXRGXXGPXAGXXGXPXXXXAXAXXPAXGXGGXRGGA 603
G G GGG G RGGA G G G G G + G GG +GGA
Sbjct: 23 GGRGGFGGGRGGARGGGRGGARGGRGGRGGARGGRGG-----------SSGGRGGAKGGA 71
Score = 27.1 bits (57), Expect = 4.3
Identities = 15/43 (34%), Positives = 16/43 (37%)
Frame = +1
Query: 475 RGGAAAGXRGXXGPXAGXXGXPXXXXAXAXXPAXGXGGXRGGA 603
RGG+ G G G G G A G G RGGA
Sbjct: 11 RGGSRGGRGGFNGGRGGFGGGRGGARGGGRGGARGGRGGRGGA 53
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 30.3 bits (65), Expect = 0.46
Identities = 20/63 (31%), Positives = 22/63 (34%), Gaps = 3/63 (4%)
Frame = -1
Query: 602 APPRXPPXPXAGXXAXAXXXX-GXPXXPAXG--PXXPRXPAAAPPRXXXXQXPPPPPXPX 432
AP PP P + G P PA P P+ AAPP PP P P
Sbjct: 1059 APSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPS 1118
Query: 431 XNP 423
P
Sbjct: 1119 GAP 1121
Score = 26.2 bits (55), Expect = 7.6
Identities = 19/63 (30%), Positives = 20/63 (31%), Gaps = 3/63 (4%)
Frame = -1
Query: 602 APPRXPPXPX-AGXXAXAXXXXGXPXXPAXGPXXPRXPA--AAPPRXXXXQXPPPPPXPX 432
AP PP P +G P P P PA APP PP P P
Sbjct: 1078 APSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPS 1137
Query: 431 XNP 423
P
Sbjct: 1138 GAP 1140
Score = 26.2 bits (55), Expect = 7.6
Identities = 11/30 (36%), Positives = 13/30 (43%)
Frame = -1
Query: 524 PAXGPXXPRXPAAAPPRXXXXQXPPPPPXP 435
P+ P P+ AAPP PP P P
Sbjct: 1155 PSGAPPVPKPSVAAPPVPAPSSGIPPVPKP 1184
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 29.9 bits (64), Expect = 0.61
Identities = 14/32 (43%), Positives = 14/32 (43%)
Frame = +1
Query: 418 RXGFXXGXGGGGGXXXXXXRGGAAAGXRGXXG 513
R GF G GG G RGG G RG G
Sbjct: 140 RGGFRGGRGGSRGGFGGNSRGGFGGGSRGGFG 171
Score = 25.8 bits (54), Expect = 10.0
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = +2
Query: 221 GXGXRGXXGGGGRGG 265
G G RG GGG RGG
Sbjct: 163 GGGSRGGFGGGSRGG 177
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 29.5 bits (63), Expect = 0.81
Identities = 18/56 (32%), Positives = 19/56 (33%)
Frame = -1
Query: 602 APPRXPPXPXAGXXAXAXXXXGXPXXPAXGPXXPRXPAAAPPRXXXXQXPPPPPXP 435
+PP PP A A P GP P PP PPPPP P
Sbjct: 731 SPPPPPPAVIVPTPAPAPIPVPPPAPIMGGP-----PPPPPPPGVAGAGPPPPPPP 781
Score = 27.9 bits (59), Expect = 2.5
Identities = 14/36 (38%), Positives = 14/36 (38%), Gaps = 2/36 (5%)
Frame = -3
Query: 750 PXPRPGXXPXPPXXXRRGGXXAPGXXXG--GXXPPP 649
P P P P PP GG P G G PPP
Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPP 777
Score = 27.5 bits (58), Expect = 3.3
Identities = 9/15 (60%), Positives = 9/15 (60%)
Frame = -2
Query: 265 PPPPPPPXXAPXPXP 221
PPPPPP P P P
Sbjct: 732 PPPPPPAVIVPTPAP 746
Score = 26.2 bits (55), Expect = 7.6
Identities = 11/18 (61%), Positives = 11/18 (61%), Gaps = 3/18 (16%)
Frame = -2
Query: 265 PPPPPPPXXA---PXPXP 221
PPPPPPP A P P P
Sbjct: 762 PPPPPPPGVAGAGPPPPP 779
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 29.1 bits (62), Expect = 1.1
Identities = 18/59 (30%), Positives = 18/59 (30%)
Frame = +1
Query: 424 GFXXGXGGGGGXXXXXXRGGAAAGXRGXXGPXAGXXGXPXXXXAXAXXPAXGXGGXRGG 600
GF G GG GG G G G G G P G GG GG
Sbjct: 206 GFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGG 264
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 28.7 bits (61), Expect = 1.4
Identities = 17/60 (28%), Positives = 19/60 (31%), Gaps = 1/60 (1%)
Frame = -1
Query: 599 PPRXPPXPXAGXXAXAXXXXGXPXXPAXGPXXPRXPAA-APPRXXXXQXPPPPPXPXXNP 423
PP PP P A A + P P P + PPPPP P P
Sbjct: 189 PPPPPPPPPAVEDQAADANEPDDYYSSGRAVSPEIPPTYTPKQADPLPAPPPPPPPTLPP 248
>SPBP8B7.26 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 262
Score = 27.9 bits (59), Expect = 2.5
Identities = 13/34 (38%), Positives = 15/34 (44%), Gaps = 1/34 (2%)
Frame = -1
Query: 533 PXXPAXG-PXXPRXPAAAPPRXXXXQXPPPPPXP 435
P P+ G P P P + P Q PPPP P
Sbjct: 112 PALPSRGTPSLPSRPGSRPSVLNQEQVPPPPVRP 145
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 27.5 bits (58), Expect = 3.3
Identities = 12/24 (50%), Positives = 12/24 (50%), Gaps = 3/24 (12%)
Frame = -2
Query: 274 NXXPPPPPP---PXXAPXPXPXXG 212
N PPPPPP P P P P G
Sbjct: 8 NPPPPPPPPGFEPPSQPPPPPPPG 31
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 27.1 bits (57), Expect = 4.3
Identities = 18/65 (27%), Positives = 21/65 (32%), Gaps = 2/65 (3%)
Frame = -1
Query: 602 APPRXPPXPX-AGXXAXAXXXXGXPXXPAXGPXX-PRXPAAAPPRXXXXQXPPPPPXPXX 429
+P PP P A + P P P P P+A PP PP P
Sbjct: 153 SPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPPLSQAPVA 212
Query: 428 NPXRR 414
N R
Sbjct: 213 NTSSR 217
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.6 bits (56), Expect = 5.7
Identities = 16/56 (28%), Positives = 18/56 (32%)
Frame = -1
Query: 602 APPRXPPXPXAGXXAXAXXXXGXPXXPAXGPXXPRXPAAAPPRXXXXQXPPPPPXP 435
AP P + A P P P P +APP PPPP P
Sbjct: 1682 APAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVP-PPPSAPPMPAGPPSAPPPPLP 1736
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.311 0.149 0.512
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,690,586
Number of Sequences: 5004
Number of extensions: 22913
Number of successful extensions: 239
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 33
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 541237406
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.6 bits)
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