Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP08_F_L17
(888 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC637.08 |||iron-sulfur cluster assembly ATPase Nbp35|Schizosa... 29 1.2
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 29 1.2
SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |S... 28 2.0
SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase ... 27 3.6
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 27 3.6
SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb... 26 6.2
SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb... 26 6.2
SPBC3D6.03c |||tRNA endonuclease |Schizosaccharomyces pombe|chr ... 26 8.2
SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ... 26 8.2
>SPAC637.08 |||iron-sulfur cluster assembly ATPase
Nbp35|Schizosaccharomyces pombe|chr 1|||Manual
Length = 317
Score = 28.7 bits (61), Expect = 1.2
Identities = 24/103 (23%), Positives = 41/103 (39%), Gaps = 1/103 (0%)
Frame = +3
Query: 174 CPNFDLNCIREYFSRNSQCQLVRGPVPDPLPLSYYRVDIPNSNLTVEYHDVKTRGFDT-I 350
CPN + I + RGP + L + + D+ NL D D +
Sbjct: 129 CPNLAVMSIGFLLPSEDSSVIWRGPKKNGLIKQFIK-DVNWENLDYLIVDTPPGTSDEHL 187
Query: 351 KIIEFYINSKTEKLVLAAEVQSLKLASPKTIFKYNRKAKEPIV 479
+++F+ NS + V+ Q + L + + RKA PI+
Sbjct: 188 SLVQFFKNSGIDGAVVVTTPQEVALQDVRKEIDFCRKASIPIL 230
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 28.7 bits (61), Expect = 1.2
Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = +3
Query: 162 IKXPCPN-FDLNCIREYFSRNSQCQLVRGPVPDP 260
I+ PC + F NCI+ + N C + R PV DP
Sbjct: 410 IQLPCKHYFHENCIKPWLRVNGTCAICRAPV-DP 442
>SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1052
Score = 27.9 bits (59), Expect = 2.0
Identities = 16/47 (34%), Positives = 28/47 (59%)
Frame = +3
Query: 567 EVFSYVHEINPKFILGPLLSFSLDSETQSQLGKLLNNIAVSLQEVFE 707
+ + ++H IN F+L LLS SL + SQ KLL +++ +++ E
Sbjct: 878 KTYGHLHYIN--FVLEKLLSSSLLTYNSSQRDKLLYEVSLLFKDLQE 922
>SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase
Cho2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 905
Score = 27.1 bits (57), Expect = 3.6
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -1
Query: 516 LEFHSQLPMRHFLQLALWLSYC 451
LEF+S L RHF+ L L +C
Sbjct: 199 LEFNSWLVFRHFVDLILMCDFC 220
>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7
domain|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1811
Score = 27.1 bits (57), Expect = 3.6
Identities = 25/90 (27%), Positives = 43/90 (47%), Gaps = 6/90 (6%)
Frame = +3
Query: 354 IIEFYIN-----SKTEKLVLAAEVQSLKLASPKTIFKYNRKAKEPIVRSDALEVDYGTLT 518
+IE Y+N TE + A V ++AS T + + R D L +D
Sbjct: 527 LIELYLNYDCISGNTENIYERAIVTLSRIASQST----SDPPPSFVFRDDQLVIDKPGF- 581
Query: 519 FTAVFPSISDL-QLSNAEVFSYVHEINPKF 605
V+ +++D+ QL+++ + SYVH NP +
Sbjct: 582 ---VYHTLNDIPQLNSSTIGSYVHSHNPPY 608
>SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 952
Score = 26.2 bits (55), Expect = 6.2
Identities = 26/114 (22%), Positives = 54/114 (47%), Gaps = 1/114 (0%)
Frame = +3
Query: 399 AAEVQSLKLASPKTIFKYNRKAKEPIVRSDALEVDYGTLTFTAVF-PSISDLQLSNAEVF 575
A E + L+++ T F +RK + I + + ++ L + ++ ++ + + F
Sbjct: 76 AREEKDLQVSRRDTCFYCSRKIIKCICNEEHVGIESVQLKLILLLCQNLPNVTTNAKKYF 135
Query: 576 SYVHEINPKFILGPLLSFSLDSETQSQLGKLLNNIAVSLQEVFEAQGTILMTSY 737
S + + F L L FSLD++T SQL + A +L E+ + +L ++
Sbjct: 136 SSLAD-GHNFTL-TLYKFSLDNQTFSQLLSRFKSFA-TLTELLQVHNVMLQVNF 186
>SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 741
Score = 26.2 bits (55), Expect = 6.2
Identities = 11/31 (35%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
Frame = +3
Query: 165 KXPCPN-FDLNCIREYFSRNSQCQLVRGPVP 254
K PC + F NC++++ + C L R VP
Sbjct: 124 KMPCGHIFGKNCLQKWLENHCTCPLCRKEVP 154
>SPBC3D6.03c |||tRNA endonuclease |Schizosaccharomyces pombe|chr
2|||Manual
Length = 678
Score = 25.8 bits (54), Expect = 8.2
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +3
Query: 558 SNAEVFSYVHEINPKFILGPLLSFSLDS 641
+N + S V +NP ++ PLL SLD+
Sbjct: 44 TNRIMLSVVSSLNPDSLIAPLLCVSLDN 71
>SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor
Ste6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 911
Score = 25.8 bits (54), Expect = 8.2
Identities = 20/82 (24%), Positives = 34/82 (41%)
Frame = +3
Query: 132 TWIRPMTQKNIKXPCPNFDLNCIREYFSRNSQCQLVRGPVPDPLPLSYYRVDIPNSNLTV 311
T I+P K FDLN + S+ C L++ P+PL + + N +
Sbjct: 428 TNIQPELNDAEKSQLDGFDLNKTNWFDSKGLVCYLMKQTSPEPLLIRNLLFSFWSCNGKI 487
Query: 312 EYHDVKTRGFDTIKIIEFYINS 377
E D K + + II + + +
Sbjct: 488 E-QDGKIKTATLVFIINYLLRT 508
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,788,436
Number of Sequences: 5004
Number of extensions: 50303
Number of successful extensions: 149
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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