Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP08_F_K02
(848 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q2F5L0 Cluster: Signal sequence receptor; n=2; Endopter... 315 8e-85
UniRef50_Q9W335 Cluster: CG32701-PA; n=5; Endopterygota|Rep: CG3... 153 5e-36
UniRef50_P43307 Cluster: Translocon-associated protein subunit a... 130 6e-29
UniRef50_A7RRI8 Cluster: Predicted protein; n=1; Nematostella ve... 113 5e-24
UniRef50_Q95XT5 Cluster: Putative uncharacterized protein; n=2; ... 107 3e-22
UniRef50_UPI0000E46DC1 Cluster: PREDICTED: hypothetical protein;... 94 5e-18
UniRef50_Q54R45 Cluster: Putative uncharacterized protein; n=1; ... 67 6e-10
UniRef50_Q4T9K6 Cluster: Chromosome undetermined SCAF7543, whole... 56 9e-07
UniRef50_P45434 Cluster: Translocon-associated protein subunit a... 53 8e-06
UniRef50_Q86FE2 Cluster: Clone ZZD1494 mRNA sequence; n=2; Schis... 52 1e-05
UniRef50_Q4T5B3 Cluster: Chromosome undetermined SCAF9335, whole... 50 7e-05
UniRef50_A4RX78 Cluster: Predicted protein; n=2; Ostreococcus|Re... 50 7e-05
UniRef50_Q4E1C2 Cluster: Putative uncharacterized protein; n=1; ... 36 1.7
>UniRef50_Q2F5L0 Cluster: Signal sequence receptor; n=2;
Endopterygota|Rep: Signal sequence receptor - Bombyx
mori (Silk moth)
Length = 279
Score = 315 bits (774), Expect = 8e-85
Identities = 155/186 (83%), Positives = 156/186 (83%)
Frame = +3
Query: 147 MXLXXFTFLIVPAALLCLGSGNILAXAEXXXXXXXXXIEGEDNQVVGDDALVDDDESVVK 326
M L FTFLIVPAALLCLGSGNILA AE IEGEDNQVVGDDALVDDDESVVK
Sbjct: 1 MKLIYFTFLIVPAALLCLGSGNILARAEEDELDDVVDIEGEDNQVVGDDALVDDDESVVK 60
Query: 327 SSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVXYVVESMEASFRYPMDY 506
SSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSV YVVESMEASFRYPMDY
Sbjct: 61 SSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMDY 120
Query: 507 TYYIQNFTALPYNRXVXPXQEATFAYSFIPNEXXAGXPFGLXVQLNYXXAXGXAYXXXVY 686
TYYIQNFTALPYNR V P QEATFAYSFIPNE AG PFGL VQLNY A G AY VY
Sbjct: 121 TYYIQNFTALPYNREVKPKQEATFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVY 180
Query: 687 NXTLTL 704
N TL +
Sbjct: 181 NQTLNI 186
>UniRef50_Q9W335 Cluster: CG32701-PA; n=5; Endopterygota|Rep:
CG32701-PA - Drosophila melanogaster (Fruit fly)
Length = 302
Score = 153 bits (371), Expect = 5e-36
Identities = 78/184 (42%), Positives = 110/184 (59%), Gaps = 3/184 (1%)
Frame = +3
Query: 162 FTFLIVPAALLCLGSG--NILAXAEXXXXXXXXXIEGEDNQVVGDDALVDDDESVVKSSQ 335
F L+V ++C S A A +EGE+ V G+DA D+D SS
Sbjct: 6 FLMLMVLPIVICTFSAPNKFGAYAAEAVEDDLVDVEGEEGAVTGEDAEADEDSDTGSSSS 65
Query: 336 -DIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVXYVVESMEASFRYPMDYTY 512
+ DT +LFTKP+ + G D+ G PVEF +GF NKG+ +V+E++EASFRYPMD+ Y
Sbjct: 66 PNADTYLLFTKPLYTPGQ-QLDLPGGKPVEFLIGFTNKGAEEFVIETVEASFRYPMDFNY 124
Query: 513 YIQNFTALPYNRXVXPXQEATFAYSFIPNEXXAGXPFGLXVQLNYXXAXGXAYXXXVYNX 692
+IQNF+A+ YNR V P E+T +Y+F+P++ AG PFGL + L Y A G Y V+N
Sbjct: 125 FIQNFSAVAYNREVKPGFESTVSYTFLPSDQFAGRPFGLNIALAYRDANGIQYNEAVFNE 184
Query: 693 TLTL 704
T+ +
Sbjct: 185 TVLI 188
>UniRef50_P43307 Cluster: Translocon-associated protein subunit
alpha precursor; n=51; Euteleostomi|Rep:
Translocon-associated protein subunit alpha precursor -
Homo sapiens (Human)
Length = 286
Score = 130 bits (313), Expect = 6e-29
Identities = 84/198 (42%), Positives = 108/198 (54%), Gaps = 14/198 (7%)
Frame = +3
Query: 153 LXXFTFLIVPAALLCLGSGN-ILAXA----EXXXXXXXXXIEGEDNQV-VGDDA---LVD 305
L L+ PA +L G +LA A E IE ED++ V +D LV+
Sbjct: 7 LLLLLLLVFPATVLFRGGPRGLLAVAQDLTEDEETVEDSIIEDEDDEAEVEEDEPTDLVE 66
Query: 306 DDESV-----VKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVXYVVE 470
D E ++S DTTILF K G+ D A V+F VGF NKG+ ++VE
Sbjct: 67 DKEEEDVSGEPEASPSADTTILFVK-----GE---DFPANNIVKFLVGFTNKGTEDFIVE 118
Query: 471 SMEASFRYPMDYTYYIQNFTALPYNRXVXPXQEATFAYSFIPNEXXAGXPFGLXVQLNYX 650
S++ASFRYP DY +YIQNFTALP N V P ++ATF YSFIP E G PFGL + LNY
Sbjct: 119 SLDASFRYPQDYQFYIQNFTALPLNTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYK 178
Query: 651 XAXGXAYXXXVYNXTLTL 704
G + V+N T+T+
Sbjct: 179 DLNGNVFQDAVFNQTVTV 196
>UniRef50_A7RRI8 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 291
Score = 113 bits (272), Expect = 5e-24
Identities = 58/134 (43%), Positives = 73/134 (54%)
Frame = +3
Query: 303 DDDESVVKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVXYVVESMEA 482
++ E +K S D DT ILFTK AG + V F N G ++V+ MEA
Sbjct: 74 EEKEDALKPSPDADTVILFTKNTEQ------QFSAGLVSKCLVSFSNNGRNDFMVDMMEA 127
Query: 483 SFRYPMDYTYYIQNFTALPYNRXVXPXQEATFAYSFIPNEXXAGXPFGLXVQLNYXXAXG 662
S RYP DY+YYIQNFTA YN V P +A+F Y+F P+E G PFGL + L Y G
Sbjct: 128 SLRYPQDYSYYIQNFTAFQYNTVVKPGHQASFEYAFRPHESFGGRPFGLSINLLYKDTDG 187
Query: 663 XAYXXXVYNXTLTL 704
Y V+N T+ L
Sbjct: 188 KMYRDAVFNETVQL 201
>UniRef50_Q95XT5 Cluster: Putative uncharacterized protein; n=2;
Caenorhabditis|Rep: Putative uncharacterized protein -
Caenorhabditis elegans
Length = 257
Score = 107 bits (258), Expect = 3e-22
Identities = 51/134 (38%), Positives = 76/134 (56%)
Frame = +3
Query: 303 DDDESVVKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVXYVVESMEA 482
+DD+ + +S D F +P S ++ + G PV++ +GF NKG +VV+ E
Sbjct: 37 EDDDLTIGASPDAGLAFHFVQP--SDANVVREFYTGKPVKYLIGFQNKGEKDFVVKYAET 94
Query: 483 SFRYPMDYTYYIQNFTALPYNRXVXPXQEATFAYSFIPNEXXAGXPFGLXVQLNYXXAXG 662
SFR+P D++Y++QNFT YNR V P +E T Y F +E AG P GL V ++Y A G
Sbjct: 95 SFRFPTDHSYHLQNFTRGEYNRRVAPKEEVTLDYGFYAHETFAGRPVGLVVNVHYQDADG 154
Query: 663 XAYXXXVYNXTLTL 704
+ VYN T+ +
Sbjct: 155 NVFVNNVYNQTINI 168
>UniRef50_UPI0000E46DC1 Cluster: PREDICTED: hypothetical protein;
n=1; Strongylocentrotus purpuratus|Rep: PREDICTED:
hypothetical protein - Strongylocentrotus purpuratus
Length = 247
Score = 93.9 bits (223), Expect = 5e-18
Identities = 48/121 (39%), Positives = 69/121 (57%)
Frame = +3
Query: 285 GDDALVDDDESVVKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVXYV 464
G++ +++E +K+S D DTT LFT + G+ +I P++ VGF N G +
Sbjct: 76 GEEEEEEEEEPPLKASPDADTTFLFT----TNGEK--EIPVTIPLQVLVGFTNNGQQDFT 129
Query: 465 VESMEASFRYPMDYTYYIQNFTALPYNRXVXPXQEATFAYSFIPNEXXAGXPFGLXVQLN 644
+ES+ A+ RYP D+++YI NFT YN V P +EATF YSF E A FG +L
Sbjct: 130 IESLHATLRYPQDFSFYIYNFTEKEYNTVVEPNKEATFEYSFQAGEVFAARQFGFVFELA 189
Query: 645 Y 647
Y
Sbjct: 190 Y 190
>UniRef50_Q54R45 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 236
Score = 66.9 bits (156), Expect = 6e-10
Identities = 42/123 (34%), Positives = 59/123 (47%)
Frame = +3
Query: 279 VVGDDALVDDDESVVKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVX 458
V DD + DDE VV+++ D + F+ P D F AG VE VGF N
Sbjct: 18 VYSDDVEITDDE-VVQATPTSDVS--FSYIFPDNQDKNF--AAGSVVEVLVGFTNNADKA 72
Query: 459 YVVESMEASFRYPMDYTYYIQNFTALPYNRXVXPXQEATFAYSFIPNEXXAGXPFGLXVQ 638
+ + AS +P D++ +IQN+T + V AT AY F+P+E FGL +
Sbjct: 73 LNITHIFASLNHPQDFSVFIQNYTRGDFGIAVEKGHHATLAYRFVPSEYLEPREFGLLIS 132
Query: 639 LNY 647
L Y
Sbjct: 133 LEY 135
>UniRef50_Q4T9K6 Cluster: Chromosome undetermined SCAF7543, whole
genome shotgun sequence; n=1; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF7543,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 316
Score = 56.4 bits (130), Expect = 9e-07
Identities = 34/83 (40%), Positives = 52/83 (62%), Gaps = 3/83 (3%)
Frame = +3
Query: 258 IEGEDNQVVGDDALVDDDESVV---KSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFF 428
+E ++N + ++ +++E++V K+S DTTILF K G+ D A V+F
Sbjct: 60 VEDDENTELTEEKEDEEEEALVGEVKASPHADTTILFVK-----GE---DFPANNIVKFL 111
Query: 429 VGFINKGSVXYVVESMEASFRYP 497
+GF NKGS +VVES++ASFRYP
Sbjct: 112 LGFTNKGSENFVVESLDASFRYP 134
>UniRef50_P45434 Cluster: Translocon-associated protein subunit
alpha precursor; n=9; Magnoliophyta|Rep:
Translocon-associated protein subunit alpha precursor -
Arabidopsis thaliana (Mouse-ear cress)
Length = 258
Score = 53.2 bits (122), Expect = 8e-06
Identities = 41/141 (29%), Positives = 58/141 (41%), Gaps = 4/141 (2%)
Frame = +3
Query: 288 DDALVDDDESV---VKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVX 458
DDA+ +DD + + S ++T +F K L + AG E VG N+G
Sbjct: 44 DDAVEEDDHDLDMNLSSFPGVETVCVFPKNSAKL------VPAGEETELLVGLKNEGKTR 97
Query: 459 YVVESMEASFRYPMDYTYYIQNFTALPYNRXVXPXQ-EATFAYSFIPNEXXAGXPFGLXV 635
V + AS P D+ +QN T L N P +ATF Y F ++ F L
Sbjct: 98 VGVMGIRASVHLPYDHKLLVQNLTMLRLNNASIPTSLQATFPYIFAVSQYLQPGAFDLVG 157
Query: 636 QLNYXXAXGXAYXXXVYNXTL 698
+ Y G Y YN T+
Sbjct: 158 YIIY-DVEGKPYQSVFYNGTI 177
>UniRef50_Q86FE2 Cluster: Clone ZZD1494 mRNA sequence; n=2;
Schistosoma japonicum|Rep: Clone ZZD1494 mRNA sequence -
Schistosoma japonicum (Blood fluke)
Length = 269
Score = 52.4 bits (120), Expect = 1e-05
Identities = 35/141 (24%), Positives = 57/141 (40%), Gaps = 5/141 (3%)
Frame = +3
Query: 300 VDDDESVVKSSQDIDTTILFTKPVPSLGDLTFDIQ--AGYPVEFFVGFINKG---SVXYV 464
V ++ V K S + + F+KP L ++ AG N +
Sbjct: 42 VTNEHDVYKGSPAVSVLVAFSKPAYGLYSARREMNFPAGEVSSLVASLANTNPPDKEQFR 101
Query: 465 VESMEASFRYPMDYTYYIQNFTALPYNRXVXPXQEATFAYSFIPNEXXAGXPFGLXVQLN 644
++ +E + YP Y Y +QNFT + P QE + Y F P+ AG F L + +
Sbjct: 102 LDFIEGALHYPSYYDYRMQNFTRQRLQGTLEPGQEISLYYRFKPSPELAGRSFDLSIVVY 161
Query: 645 YXXAXGXAYXXXVYNXTLTLW 707
Y Y ++N T+ L+
Sbjct: 162 YHDNNNIYYSHKLFNQTVNLF 182
>UniRef50_Q4T5B3 Cluster: Chromosome undetermined SCAF9335, whole
genome shotgun sequence; n=8; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF9335,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 219
Score = 50.0 bits (114), Expect = 7e-05
Identities = 23/42 (54%), Positives = 26/42 (61%)
Frame = +3
Query: 522 NFTALPYNRXVXPXQEATFAYSFIPNEXXAGXPFGLXVQLNY 647
NFTAL V P ++ATF SFIP E G PFGL + LNY
Sbjct: 1 NFTALQLGTVVPPARQATFECSFIPAEPMGGAPFGLVINLNY 42
>UniRef50_A4RX78 Cluster: Predicted protein; n=2; Ostreococcus|Rep:
Predicted protein - Ostreococcus lucimarinus CCE9901
Length = 249
Score = 50.0 bits (114), Expect = 7e-05
Identities = 34/125 (27%), Positives = 56/125 (44%), Gaps = 1/125 (0%)
Frame = +3
Query: 327 SSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVXYVVESMEASFRYPMDY 506
++ D+++ + F P D F G V+ +G N G+ V + S P ++
Sbjct: 35 ANPDVESVVAF----PDHADRAFP--GGERVKVLLGLRNTGNDAVNVTHLAGSLNVPNNF 88
Query: 507 TYYIQNFTALPY-NRXVXPXQEATFAYSFIPNEXXAGXPFGLXVQLNYXXAXGXAYXXXV 683
+Y+ NFT + + + P +EATF Y F + AG L + Y A G AY
Sbjct: 89 NFYVTNFTVSSFGDAVIKPKKEATFEYEFAIDPQFAGHSLQLALTAFYDEA-GVAYATTY 147
Query: 684 YNXTL 698
+N T+
Sbjct: 148 FNSTV 152
>UniRef50_Q4E1C2 Cluster: Putative uncharacterized protein; n=1;
Trypanosoma cruzi|Rep: Putative uncharacterized protein
- Trypanosoma cruzi
Length = 228
Score = 35.5 bits (78), Expect = 1.7
Identities = 21/77 (27%), Positives = 35/77 (45%), Gaps = 2/77 (2%)
Frame = +3
Query: 480 ASFRYPMD-YTYYIQNFTALPYNRXVXPXQEATFAYSFIPNEXXAGXPFGLXVQLNY-XX 653
A + P++ Y IQNF+ + + R V P +F Y+F P+ + L + L Y
Sbjct: 62 AGYLTPLNLYGQVIQNFSVVRHARIVQPTDTTSFQYTFTPDAMLEPNDYTLILGLYYQDN 121
Query: 654 AXGXAYXXXVYNXTLTL 704
Y +N T+T+
Sbjct: 122 VTNHTYFATAFNNTVTV 138
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 532,982,684
Number of Sequences: 1657284
Number of extensions: 8343782
Number of successful extensions: 16294
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 15782
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 16268
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 74603367202
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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