Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP08_F_J17
(880 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 299 3e-82
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 294 1e-80
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 138 7e-34
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 113 4e-26
SPCC1281.04 |||pyridoxal reductase |Schizosaccharomyces pombe|ch... 27 2.7
SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyc... 27 2.7
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 27 3.5
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 27 4.7
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 299 bits (734), Expect = 3e-82
Identities = 146/246 (59%), Positives = 172/246 (69%), Gaps = 2/246 (0%)
Frame = +3
Query: 147 MRXCISVHVGQAGVQXGNACWXLYCLEHGIQPDGQMPTDKXIGGGXDSFNTFFSETGAGK 326
MR IS+HVGQAG Q GNACW LYCLEHGIQP+G M + F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 327 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 506
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 507 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLGVRHLPRASGFHC 686
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL P
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAP---QV 177
Query: 687 RRRALQLYXXXXXXXXAL*L--CFQWVDNEAIYDICRRNLDIERPTYTNLNRLIGQXVSS 860
++ Y L L C VDNE+ YDICRRNLDIERP+Y NLNRLI Q VSS
Sbjct: 178 STSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSS 237
Query: 861 ITXSLR 878
IT SLR
Sbjct: 238 ITASLR 243
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 294 bits (721), Expect = 1e-80
Identities = 149/251 (59%), Positives = 173/251 (68%), Gaps = 7/251 (2%)
Frame = +3
Query: 147 MRXCISVHVGQAGVQXGNACWXLYCLEHGIQPDGQMPTD-----KXIGGGXDSFNTFFSE 311
MR ISVHVGQAGVQ GNACW LYCLEHGI PDG PT+ K D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 312 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 491
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 492 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLGVRHLPRA 671
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L P
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 672 SGFHCRRRALQLYXXXXXXXXAL*L--CFQWVDNEAIYDICRRNLDIERPTYTNLNRLIG 845
++ Y L C VDNEA YDICRRNLDIERPTY NLNRLI
Sbjct: 180 ---QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIA 236
Query: 846 QXVSSITXSLR 878
Q VSSIT SLR
Sbjct: 237 QVVSSITASLR 247
Score = 65.7 bits (153), Expect = 8e-12
Identities = 26/37 (70%), Positives = 33/37 (89%)
Frame = +1
Query: 643 NWEFAIYPAPQVSTAVVEPYNSILTTHTXLEHSDCAF 753
N +F++YPAPQVST+VVEPYNS+LTTH L++SDC F
Sbjct: 170 NLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTF 206
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 138 bits (335), Expect = 7e-34
Identities = 80/246 (32%), Positives = 118/246 (47%), Gaps = 2/246 (0%)
Frame = +3
Query: 147 MRXCISVHVGQAGVQXGNACWXLYCLEHGIQPDGQMPTDKXIGGGXDSFNTFFSETGAGK 326
MR + + GQ G Q G A W EHG+ G + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSE--AQHERLNVYFNEAAGGK 58
Query: 327 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 506
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 507 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKK--SKLGVRHLPRASGF 680
+R+ A+ C LQGF + H LL+ ++ +Y + + V P++S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 681 HCRRRALQLYXXXXXXXXAL*LCFQWVDNEAIYDICRRNLDIERPTYTNLNRLIGQXVSS 860
L C +DNEA+ I L I+ P+Y +LN L+ ++
Sbjct: 179 VVEPYNATLSMHQLVENSDETFC---IDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAG 235
Query: 861 ITXSLR 878
+T S R
Sbjct: 236 VTTSFR 241
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 113 bits (271), Expect = 4e-26
Identities = 75/247 (30%), Positives = 116/247 (46%), Gaps = 4/247 (1%)
Frame = +3
Query: 150 RXCISVHVGQAGVQXGNACWXLYCLEHGIQPDGQMPTDKXIGGGXDSFNTFFSETGAGKH 329
R I++ GQ G Q G+ W CLEHGI PDG + + G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTL--ESFATEGVDRKDVFFYQSDDTRY 60
Query: 330 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 503
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 504 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLGVRHLPRASGFH 683
D I + AD L+GF + H L+ERL+ Y KK P +
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQS-- 177
Query: 684 CRRRALQLYXXXXXXXXAL*LCFQWV--DNEAIYDICRRNLDIERPTYTNLNRLIGQXVS 857
+Q Y V DN A+ I L + PT+ N+L+ +S
Sbjct: 178 VSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMS 237
Query: 858 SITXSLR 878
+ T +LR
Sbjct: 238 ASTTTLR 244
Score = 26.2 bits (55), Expect = 6.1
Identities = 11/22 (50%), Positives = 17/22 (77%), Gaps = 1/22 (4%)
Frame = +1
Query: 652 FAIYPAPQ-VSTAVVEPYNSIL 714
++++P Q VS VV+PYNS+L
Sbjct: 169 YSVFPNSQSVSDVVVQPYNSLL 190
>SPCC1281.04 |||pyridoxal reductase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 333
Score = 27.5 bits (58), Expect = 2.7
Identities = 16/36 (44%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = -2
Query: 786 RCHRWLHCRPIESTVRVLKXCV-GGEDRVVGLDDGS 682
+C R H PIE+T++ LK V GE VGL + S
Sbjct: 121 QCARVDHKVPIETTMKALKAFVDSGEISCVGLSEAS 156
>SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 27.5 bits (58), Expect = 2.7
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = +1
Query: 682 TAVVEPYNSILTTHTXLEHSDCAFNGSTMKP 774
T +V YN +T + LEH + A G ++ P
Sbjct: 812 TEIVRQYNENVTLYAPLEHINVAIRGGSVLP 842
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 27.1 bits (57), Expect = 3.5
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +1
Query: 700 YNSILTTHTXLEHSDCAFNGSTMKPSMTSAAVISTLSAQPT 822
YN+ + + L S A + T S+ S++ ST SA PT
Sbjct: 65 YNTSSASSSSLTSSSAASSSLTSSSSLASSSTNSTTSASPT 105
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 26.6 bits (56), Expect = 4.7
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = -3
Query: 875 QRXSDRGDXLSDETIQVGVGWALNVEITAADVIDG 771
Q+ SD + +D T VG+G AL I + D +DG
Sbjct: 184 QKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,122,568
Number of Sequences: 5004
Number of extensions: 59615
Number of successful extensions: 183
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 175
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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