Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP08_F_I18
         (890 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz...    88   1e-18
SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac...    58   2e-09
SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz...    54   3e-08
SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa...    39   0.001
SPAC186.05c |||human TMEM165 homolog|Schizosaccharomyces pombe|c...    28   2.1  
SPCC1322.16 |phb2||prohibitin Phb2|Schizosaccharomyces pombe|chr...    27   4.7  
SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p...    26   8.3  
SPBC23E6.04c |utp10||U3 snoRNP-associated protein Utp10 |Schizos...    26   8.3  

>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 216

 Score = 88.2 bits (209), Expect = 1e-18
 Identities = 51/155 (32%), Positives = 79/155 (50%), Gaps = 4/155 (2%)
 Frame = +3

Query: 285 QRVDYIIKDSKGHILSQKDTVTKGK-FSFVTENYDMFEVCFISKVPSERRGIPHQ---VS 452
           Q +  +I DS G+  S    V   K  +F  +   M ++CF++ +        H+   V 
Sbjct: 61  QTLSMMITDSSGNTHSSIQNVLGEKSVAFDVDASAMLDICFLNTLTPGAIESEHKKRSVK 120

Query: 453 LDIKIGIEAKTYEGIGEAAKLKPMEVELKRLEDLSEAIVQDFTLMRKREXEMRDTNESTN 632
           L+  +G +A  Y  + +A  L+P+E +++R  D  E I      ++ RE   R+TNESTN
Sbjct: 121 LEFTVGADADDYSSLQKANNLEPVEADIRRARDFIEEIKGKIYYLQAREARFRNTNESTN 180

Query: 633 NRVLFFSIFSMACLLGLATWQVLYLRRFFKAKKLI 737
            RV  F+  +   L  L  WQ+LYLR FF+ K LI
Sbjct: 181 ERVKNFAYLTFISLFVLVIWQILYLRSFFQRKHLI 215


>SPBC16E9.09c |||COPII vesicle coat component
           Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 215

 Score = 57.6 bits (133), Expect = 2e-09
 Identities = 36/142 (25%), Positives = 65/142 (45%)
 Frame = +3

Query: 312 SKGHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHQVSLDIKIGIEAKTYE 491
           +  H++ + D  +     F T N     +C++S           +  L + +  + K   
Sbjct: 75  ANNHLIYKNDLRSNEAVRFTTTNEGTHRICYLSTSDGAWFSST-KTRLHVDLASDDKYNL 133

Query: 492 GIGEAAKLKPMEVELKRLEDLSEAIVQDFTLMRKREXEMRDTNESTNNRVLFFSIFSMAC 671
                 + K +   +  L    E+I+ +  L R RE   RDT+ES N+RV+ ++I  +  
Sbjct: 134 IASSEDEAKDLTDRVAALNTRLESILGEQMLQRSREMTFRDTSESANSRVVRWTIVQIVV 193

Query: 672 LLGLATWQVLYLRRFFKAKKLI 737
           LL    WQ+ +L+RFF  +KL+
Sbjct: 194 LLVTCIWQLSHLQRFFVKEKLV 215


>SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 199

 Score = 54.0 bits (124), Expect = 3e-08
 Identities = 35/151 (23%), Positives = 68/151 (45%)
 Frame = +3

Query: 285 QRVDYIIKDSKGHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHQVSLDIK 464
           Q V   I +  G I+ Q+   +  ++SF  +N   +  CF +             S ++ 
Sbjct: 56  QLVSMSIYNPAGQIMHQEVPNSMAQYSFTVKNPGKYMYCFYNDALDGE-------SKEVL 108

Query: 465 IGIEAKTYEGIGEAAKLKPMEVELKRLEDLSEAIVQDFTLMRKREXEMRDTNESTNNRVL 644
             +    Y    +     P+  ++++L D    +  +      RE   R+T ESTN+RV 
Sbjct: 109 FNVHGVIYISDEDLDANNPLLGKVRQLHDTISKVKHEQEYFVARERIHRNTAESTNDRVK 168

Query: 645 FFSIFSMACLLGLATWQVLYLRRFFKAKKLI 737
           ++SI     L+ +  +Q+ YL+R F+ K+++
Sbjct: 169 WWSILQTVILVSVCVFQIFYLKRLFEVKRVV 199


>SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 210

 Score = 38.7 bits (86), Expect = 0.001
 Identities = 33/146 (22%), Positives = 66/146 (45%), Gaps = 2/146 (1%)
 Frame = +3

Query: 291 VDYIIKDSKGHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPS-ERRGIPHQVSLDIKI 467
           VDY+IK      ++      +    F  E    +E CF + + +   + +  +++++ ++
Sbjct: 60  VDYMIKAPSQKTVALGKKRRQADVFFTLEEKGEYEFCFDNHMSTFTDKIVTMEITMENEL 119

Query: 468 GIEAKTYEGIGEAAKLKPMEVELKRLEDLSEAI-VQDFTLMRKREXEMRDTNESTNNRVL 644
            + A T +   +  K       L+    LSE   VQ++   + RE     T +ST  R+ 
Sbjct: 120 SLPALTRDEAKDYKKDSMQSTVLEISTALSEIDRVQNY--FKTREHRNYSTVKSTQARIF 177

Query: 645 FFSIFSMACLLGLATWQVLYLRRFFK 722
           +FS+     ++ L+  QV  ++ FFK
Sbjct: 178 WFSLAESIMVVALSALQVFIVKTFFK 203


>SPAC186.05c |||human TMEM165 homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 262

 Score = 27.9 bits (59), Expect = 2.1
 Identities = 23/81 (28%), Positives = 41/81 (50%), Gaps = 2/81 (2%)
 Frame = +3

Query: 501 EAAKLKPMEVELKRLEDLSEAIVQDFTLMRKR-EXEMRDTNESTNN-RVLFFSIFSMACL 674
           E+ +++  +  L+   D  E I+ +   M+K  E  +  +N S++  +  FF +FSM+C 
Sbjct: 108 ESKEVRESQQSLENEFDKVEKIIVNEEDMKKTLELGLPASNRSSSTLKDKFFKVFSMSCF 167

Query: 675 LGLATWQVLYLRRFFKAKKLI 737
             L  +   + R F KA  LI
Sbjct: 168 KNL--FSKKFSRAFIKAFALI 186


>SPCC1322.16 |phb2||prohibitin Phb2|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 279

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 13/29 (44%), Positives = 18/29 (62%)
 Frame = +3

Query: 474 EAKTYEGIGEAAKLKPMEVELKRLEDLSE 560
           E +  + IGEA K KP  +EL++LE   E
Sbjct: 226 EGRAAQLIGEAIKNKPGFIELRKLETARE 254


>SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 425

 Score = 25.8 bits (54), Expect = 8.3
 Identities = 8/20 (40%), Positives = 12/20 (60%)
 Frame = +2

Query: 203 NTHKCLKEELHANVLVAGEY 262
           NTH+C++     N  + GEY
Sbjct: 258 NTHRCIERSTDCNCPICGEY 277


>SPBC23E6.04c |utp10||U3 snoRNP-associated protein Utp10
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1649

 Score = 25.8 bits (54), Expect = 8.3
 Identities = 13/28 (46%), Positives = 17/28 (60%)
 Frame = +3

Query: 522 MEVELKRLEDLSEAIVQDFTLMRKREXE 605
           M++ L RLE LSE   + F  + KRE E
Sbjct: 654 MQILLPRLEQLSEFNSEKFKTVSKREVE 681


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,228,115
Number of Sequences: 5004
Number of extensions: 62232
Number of successful extensions: 131
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)