Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP08_F_G16
(921 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 36 0.008
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 28 2.1
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 2.1
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 27 3.7
SPBC83.12 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M... 26 6.5
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 6.5
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 8.6
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 35.9 bits (79), Expect = 0.008
Identities = 20/52 (38%), Positives = 20/52 (38%)
Frame = +2
Query: 650 PPPXXXXPXXGXAPPNXXXXPPKXXXXGGPPPXXXXXPPQKXXXXXXPPPPP 805
PPP P AP PP GGPPP PP PPPPP
Sbjct: 735 PPPAVIVPTPAPAP---IPVPPPAPIMGGPPP----PPPPPGVAGAGPPPPP 779
Score = 32.7 bits (71), Expect = 0.075
Identities = 14/38 (36%), Positives = 14/38 (36%)
Frame = +1
Query: 736 PPPPXXXAXPPKXXXXXXXPPPPPXXXXXXGXFXPPPP 849
P P PP PPPPP G PPPP
Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 29.5 bits (63), Expect = 0.70
Identities = 16/63 (25%), Positives = 19/63 (30%)
Frame = +3
Query: 711 PXKXXXLXAPPPXFXXXPPKKXXGAXXPPPPPXXXKXGXXFXSPPXAXPXXXXXXTKKXX 890
P + P P PP PPPPP G PP P ++
Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYA 794
Query: 891 PPP 899
P P
Sbjct: 795 PAP 797
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 27.9 bits (59), Expect = 2.1
Identities = 18/54 (33%), Positives = 19/54 (35%)
Frame = -1
Query: 804 GGGGGXXXXXXFWGGXXXXXGGGPPXKXXXGGXXXXFGGAXPXXGXXXXGGGXP 643
GG GG + G GGGP GG FGG G G G P
Sbjct: 221 GGHGGFGGGPGGFEGGPGGFGGGP---GGFGGGLGGFGGGPGGFGGGPGGHGGP 271
Score = 25.8 bits (54), Expect = 8.6
Identities = 15/42 (35%), Positives = 16/42 (38%)
Frame = -1
Query: 768 WGGXXXXXGGGPPXKXXXGGXXXXFGGAXPXXGXXXXGGGXP 643
+GG GG PP GG FGG G GG P
Sbjct: 190 FGGFGGGSGGPPPGPGGFGG-FGGFGGEGHHHGGHGGFGGGP 230
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.9 bits (59), Expect = 2.1
Identities = 12/40 (30%), Positives = 15/40 (37%)
Frame = +3
Query: 738 PPPXFXXXPPKKXXGAXXPPPPPXXXKXGXXFXSPPXAXP 857
PP PP++ P PPP SPP + P
Sbjct: 233 PPSIPSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPP 272
Score = 26.2 bits (55), Expect = 6.5
Identities = 15/65 (23%), Positives = 15/65 (23%)
Frame = +2
Query: 650 PPPXXXXPXXGXAPPNXXXXPPKXXXXGGPPPXXXXXPPQKXXXXXXPPPPPXXXKXXXV 829
PPP PP PP P P PP PP
Sbjct: 341 PPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSA 400
Query: 830 XFXPP 844
PP
Sbjct: 401 PALPP 405
Score = 25.8 bits (54), Expect = 8.6
Identities = 14/39 (35%), Positives = 14/39 (35%), Gaps = 2/39 (5%)
Frame = +1
Query: 736 PPPPXXXAXP--PKXXXXXXXPPPPPXXXXXXGXFXPPP 846
PPPP A P PPPPP PPP
Sbjct: 340 PPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPP 378
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 27.1 bits (57), Expect = 3.7
Identities = 21/83 (25%), Positives = 21/83 (25%), Gaps = 4/83 (4%)
Frame = +1
Query: 613 PPXXKXXXWGXXPPTXXXXPXXXXXPPKXXXXXPXXXFXWXPPP----PXXXAXPPKXXX 780
PP PP PP P PPP P PP
Sbjct: 209 PPPMHHKPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMP--PPPMHHEPGEHMPPPPMHH 266
Query: 781 XXXXPPPPPXXXXXXGXFXPPPP 849
PPP G PPPP
Sbjct: 267 EPGEHMPPPPMHHEPGEHMPPPP 289
Score = 27.1 bits (57), Expect = 3.7
Identities = 21/83 (25%), Positives = 21/83 (25%), Gaps = 4/83 (4%)
Frame = +1
Query: 613 PPXXKXXXWGXXPPTXXXXPXXXXXPPKXXXXXPXXXFXWXPPP----PXXXAXPPKXXX 780
PP PP PP P PPP P PP
Sbjct: 222 PPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMP--PPPMHHEPGEHMPPPPMHH 279
Query: 781 XXXXPPPPPXXXXXXGXFXPPPP 849
PPP G PPPP
Sbjct: 280 EPGEHMPPPPMHHEPGEHMPPPP 302
Score = 27.1 bits (57), Expect = 3.7
Identities = 21/83 (25%), Positives = 21/83 (25%), Gaps = 4/83 (4%)
Frame = +1
Query: 613 PPXXKXXXWGXXPPTXXXXPXXXXXPPKXXXXXPXXXFXWXPPP----PXXXAXPPKXXX 780
PP PP PP P PPP P PP
Sbjct: 235 PPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMP--PPPMHHEPGEHMPPPPMHH 292
Query: 781 XXXXPPPPPXXXXXXGXFXPPPP 849
PPP G PPPP
Sbjct: 293 EPGEHMPPPPMHHEPGEHMPPPP 315
Score = 27.1 bits (57), Expect = 3.7
Identities = 21/83 (25%), Positives = 21/83 (25%), Gaps = 4/83 (4%)
Frame = +1
Query: 613 PPXXKXXXWGXXPPTXXXXPXXXXXPPKXXXXXPXXXFXWXPPP----PXXXAXPPKXXX 780
PP PP PP P PPP P PP
Sbjct: 248 PPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMP--PPPMHHEPGEHMPPPPMHH 305
Query: 781 XXXXPPPPPXXXXXXGXFXPPPP 849
PPP G PPPP
Sbjct: 306 EPGEHMPPPPMHHEPGEHMPPPP 328
Score = 27.1 bits (57), Expect = 3.7
Identities = 21/83 (25%), Positives = 21/83 (25%), Gaps = 4/83 (4%)
Frame = +1
Query: 613 PPXXKXXXWGXXPPTXXXXPXXXXXPPKXXXXXPXXXFXWXPPP----PXXXAXPPKXXX 780
PP PP PP P PPP P PP
Sbjct: 261 PPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMP--PPPMHHEPGEHMPPPPMHH 318
Query: 781 XXXXPPPPPXXXXXXGXFXPPPP 849
PPP G PPPP
Sbjct: 319 EPGEHMPPPPMHHEPGEHMPPPP 341
Score = 26.2 bits (55), Expect = 6.5
Identities = 19/71 (26%), Positives = 19/71 (26%), Gaps = 4/71 (5%)
Frame = +1
Query: 649 PPTXXXXPXXXXXPPKXXXXXPXXXFXWXPPP----PXXXAXPPKXXXXXXXPPPPPXXX 816
PP PP P PPP P PP PPP
Sbjct: 208 PPPPMHHKPGEHMPPPPMHHEPGEHMP--PPPMHHEPGEHMPPPPMHHEPGEHMPPPPMH 265
Query: 817 XXXGXFXPPPP 849
G PPPP
Sbjct: 266 HEPGEHMPPPP 276
>SPBC83.12 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 161
Score = 26.2 bits (55), Expect = 6.5
Identities = 12/42 (28%), Positives = 20/42 (47%)
Frame = -2
Query: 188 HLVKVKSLQMLNITKIVSNNQQNCGHVIMNPIMYLQYICNAE 63
HL + L ++ S + GH++ +PI Y Y+ AE
Sbjct: 72 HLFSINRYHGLRFSRQSSQEYDSVGHILYSPITY--YVSGAE 111
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 6.5
Identities = 16/56 (28%), Positives = 16/56 (28%), Gaps = 1/56 (1%)
Frame = +1
Query: 736 PPPPXXXAXPP-KXXXXXXXPPPPPXXXXXXGXFXPPPPXXLXXXXXPXKKXXXPP 900
PP P PP PPP P PP P L P PP
Sbjct: 130 PPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPP 185
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.8 bits (54), Expect = 8.6
Identities = 13/39 (33%), Positives = 13/39 (33%), Gaps = 2/39 (5%)
Frame = +1
Query: 736 PPPPXXXAXPPKXXXXXXXPP--PPPXXXXXXGXFXPPP 846
PPPP PP PP PPP P P
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,251,683
Number of Sequences: 5004
Number of extensions: 36082
Number of successful extensions: 155
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 38
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 90
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 468512460
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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