Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP08_F_F16
(875 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 75 2e-14
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 62 1e-10
SPCC1906.02c |||CUE domain protein Cue3 |Schizosaccharomyces pom... 28 1.5
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 27 2.7
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 4.6
SPAC8F11.03 |msh3|swi4|MutS protein homolog 3|Schizosaccharomyce... 27 4.6
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 8.1
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 26 8.1
SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizo... 26 8.1
>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 407
Score = 74.5 bits (175), Expect = 2e-14
Identities = 40/113 (35%), Positives = 63/113 (55%)
Frame = +2
Query: 452 LKENGIQASKDIKLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTIQSLV 631
+K N DIK+LLLGAG+SGK+TI+KQM++++ GF+ KQYR +++ N I SL
Sbjct: 64 VKGNSTSGGNDIKVLLLGAGDSGKTTIMKQMRLLYSPGFSQVVRKQYRVMIFENIISSLC 123
Query: 632 AILRAMPNLGIIYGNRDRESDGKMVSDVIXRMEDTEPFXEELLAAMKRLWADS 790
+L AM N + + + ++ + EPF E+ A+ L D+
Sbjct: 124 LLLEAMDNSNVSLLPENEKYRAVILRK--HTSQPNEPFSPEIYEAVHALTLDT 174
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 62.1 bits (144), Expect = 1e-10
Identities = 37/124 (29%), Positives = 67/124 (54%), Gaps = 2/124 (1%)
Frame = +2
Query: 440 IDKNLKENGIQASKDIKLLLLGAGESGKSTIVKQMKIIHESGFTNEDFKQYRPVVYSNTI 619
I+K ++ + K K+LLLGA +SGKSTI KQ+KI++++GF+ E+ + PV+ N +
Sbjct: 20 IEKQIENASKKDKKIYKVLLLGASDSGKSTISKQIKILNKNGFSQEEIMTFIPVIRRNLL 79
Query: 620 QSLVAILRAMPNLGIIYGNRDRESDGKMVSDVIXRMEDT--EPFXEELLAAMKRLWADSG 793
+S +++ + GI + + G ++I + T E + A+ LW+ +
Sbjct: 80 ESAKTLVKIIVQKGI-----NLDPLGTHNCEIIEKFNPTPGELINANIGQAITSLWSANS 134
Query: 794 VXEC 805
V C
Sbjct: 135 VRSC 138
>SPCC1906.02c |||CUE domain protein Cue3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 581
Score = 28.3 bits (60), Expect = 1.5
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +2
Query: 116 SRVFYAGSILKSIDLLRCIYLLEESD 193
S VFY +L S+DL CIY++ E +
Sbjct: 198 SLVFYLEMLLDSLDLRNCIYVVFEDE 223
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 27.5 bits (58), Expect = 2.7
Identities = 9/26 (34%), Positives = 22/26 (84%)
Frame = +2
Query: 473 ASKDIKLLLLGAGESGKSTIVKQMKI 550
++K++++L+LG +GK+TI+ ++K+
Sbjct: 18 SNKEMRILMLGLDAAGKTTILYKLKL 43
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 26.6 bits (56), Expect = 4.6
Identities = 14/45 (31%), Positives = 20/45 (44%), Gaps = 2/45 (4%)
Frame = -3
Query: 390 PISAVLPMLWPQAA--PTPEPGALSPISQHALYTRPVAPALPEPP 262
P S + P+ P A P P P P+ + ++ PV P PP
Sbjct: 1174 PSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPP 1218
>SPAC8F11.03 |msh3|swi4|MutS protein homolog 3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1004
Score = 26.6 bits (56), Expect = 4.6
Identities = 16/40 (40%), Positives = 23/40 (57%), Gaps = 2/40 (5%)
Frame = +2
Query: 437 LIDKNLKENGIQASKD-IKLLLL-GAGESGKSTIVKQMKI 550
L DK+ N I S D ++ LL+ G GKS+ VKQ+ +
Sbjct: 746 LSDKSFVPNHIHLSSDGVRCLLITGPNMGGKSSFVKQLAL 785
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 25.8 bits (54), Expect = 8.1
Identities = 14/39 (35%), Positives = 18/39 (46%), Gaps = 1/39 (2%)
Frame = -3
Query: 375 LPMLWPQAAPTPEPGALS-PISQHALYTRPVAPALPEPP 262
LPM P A P P ++ P+ P+ PA P PP
Sbjct: 439 LPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPP 477
>SPAC22F3.05c |alp41||ADP-ribosylation factor
Alp41|Schizosaccharomyces pombe|chr 1|||Manual
Length = 186
Score = 25.8 bits (54), Expect = 8.1
Identities = 9/20 (45%), Positives = 17/20 (85%)
Frame = +2
Query: 479 KDIKLLLLGAGESGKSTIVK 538
+++++LLLG +GK+TI+K
Sbjct: 15 REVRVLLLGLDNAGKTTILK 34
>SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein
Sap49|Schizosaccharomyces pombe|chr 1|||Manual
Length = 335
Score = 25.8 bits (54), Expect = 8.1
Identities = 15/38 (39%), Positives = 18/38 (47%), Gaps = 2/38 (5%)
Frame = -3
Query: 348 PTPEPGALSPISQHALYTRPVAPALPEPPY--TILAPN 241
P P PG + A T P AP +P P+ TI A N
Sbjct: 290 PPPPPGMVMASPSPAAATIPGAPVMPNIPFYQTINAQN 327
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,297,766
Number of Sequences: 5004
Number of extensions: 66793
Number of successful extensions: 201
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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