Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP08_F_F08
(887 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q1HPY3 Cluster: Secreted protein acidic and rich in cys... 178 2e-43
UniRef50_Q9GR92 Cluster: SPARC precursor; n=1; Artemia francisca... 82 2e-14
UniRef50_Q8SY75 Cluster: RH45818p; n=7; Endopterygota|Rep: RH458... 57 7e-07
UniRef50_P34714 Cluster: SPARC precursor; n=3; Caenorhabditis|Re... 52 1e-05
UniRef50_Q6PVV9 Cluster: SPARC; n=1; Ciona intestinalis|Rep: SPA... 42 0.028
UniRef50_P09486 Cluster: SPARC precursor; n=15; Vertebrata|Rep: ... 36 1.0
UniRef50_Q1WIX6 Cluster: Follistatin-related protein; n=2; Haema... 36 1.8
UniRef50_P07214 Cluster: SPARC precursor; n=24; Euteleostomi|Rep... 36 1.8
UniRef50_UPI000051A338 Cluster: PREDICTED: similar to agrin isof... 35 3.2
UniRef50_Q3WGV9 Cluster: Regulatory protein, TetR; n=1; Frankia ... 35 3.2
UniRef50_Q62356 Cluster: Follistatin-related protein 1 precursor... 34 5.6
UniRef50_Q12841 Cluster: Follistatin-related protein 1 precursor... 34 5.6
>UniRef50_Q1HPY3 Cluster: Secreted protein acidic and rich in
cysteine; n=4; Neoptera|Rep: Secreted protein acidic and
rich in cysteine - Bombyx mori (Silk moth)
Length = 317
Score = 178 bits (433), Expect = 2e-43
Identities = 96/193 (49%), Positives = 99/193 (51%)
Frame = +2
Query: 122 MKGXXXXXXXXXXXCTADASXXXXXXXXXXXXXXXXXXSEQLGRGDISEAEHIEHGMSES 301
MKG CTADAS SEQLGRGDISEAEHIEHGMSES
Sbjct: 1 MKGLLALALVIAVICTADASRMRRRHRRIRTTTTERPTSEQLGRGDISEAEHIEHGMSES 60
Query: 302 LDEKRYHEAEIARXXXXXXXXXXXXXXXXXIXMEDPXPESPXXXXXXXXXXXXXXXHV*L 481
LDEKRYHEAEIAR I MEDP +
Sbjct: 61 LDEKRYHEAEIARVNDLLNEVSNEENEDEEINMEDPCLKVHCSAGRVCEINEHGDAMCNC 120
Query: 482 HXGLSLRDRLQTXGVHXLHETWQSDCEVYRXRCLCLDNSDQCRGPXYHHVXIEYYXTCRX 661
+ +ETWQSDCEVYR RCLCLDNSDQCRGP YHHV IEYY TCR
Sbjct: 121 IKDCPYETDSRRMVCTNFNETWQSDCEVYRQRCLCLDNSDQCRGPQYHHVQIEYYGTCRE 180
Query: 662 MPXCTESXMSDFP 700
MP CTES MSDFP
Sbjct: 181 MPDCTESEMSDFP 193
Score = 91.9 bits (218), Expect = 2e-17
Identities = 37/41 (90%), Positives = 38/41 (92%)
Frame = +3
Query: 420 VHCSAGRVCEINEHGDAMCNCIXDCPYETDSRRXVCTNFTK 542
VHCSAGRVCEINEHGDAMCNCI DCPYETDSRR VCTNF +
Sbjct: 100 VHCSAGRVCEINEHGDAMCNCIKDCPYETDSRRMVCTNFNE 140
>UniRef50_Q9GR92 Cluster: SPARC precursor; n=1; Artemia
franciscana|Rep: SPARC precursor - Artemia
sanfranciscana (Brine shrimp) (Artemia franciscana)
Length = 291
Score = 82.2 bits (194), Expect = 2e-14
Identities = 32/55 (58%), Positives = 37/55 (67%)
Frame = +2
Query: 536 HETWQSDCEVYRXRCLCLDNSDQCRGPXYHHVXIEYYXTCRXMPXCTESXMSDFP 700
+ET+ SDCE+YR RCLC S +C GP Y H IEYY CR MP C+E M DFP
Sbjct: 113 NETFNSDCELYRMRCLCTTGSQECLGPKYSHAHIEYYGECRDMPECSEQEMDDFP 167
Score = 50.0 bits (114), Expect = 8e-05
Identities = 19/42 (45%), Positives = 26/42 (61%)
Frame = +3
Query: 426 CSAGRVCEINEHGDAMCNCIXDCPYETDSRRXVCTNFTKPGN 551
C AG+ C+I++ G+A C C+ C E D RR VCTN + N
Sbjct: 76 CGAGKECKISDSGEAECRCVESCLPEVDDRRKVCTNHNETFN 117
>UniRef50_Q8SY75 Cluster: RH45818p; n=7; Endopterygota|Rep: RH45818p
- Drosophila melanogaster (Fruit fly)
Length = 304
Score = 56.8 bits (131), Expect = 7e-07
Identities = 22/55 (40%), Positives = 27/55 (49%)
Frame = +2
Query: 536 HETWQSDCEVYRXRCLCLDNSDQCRGPXYHHVXIEYYXTCRXMPXCTESXMSDFP 700
+ETW SDC VY+ RC C C P H+ I+YY C C + DFP
Sbjct: 126 NETWPSDCSVYQQRCWCDSGEPGCTNPDNAHMHIDYYGACHEPRSCEGEDLKDFP 180
Score = 47.2 bits (107), Expect = 6e-04
Identities = 18/36 (50%), Positives = 25/36 (69%)
Frame = +3
Query: 426 CSAGRVCEINEHGDAMCNCIXDCPYETDSRRXVCTN 533
C AGR+C++++ C CI +CP E D+RR VCTN
Sbjct: 90 CGAGRICQMHDE-KPKCVCIPECPEEVDTRRLVCTN 124
>UniRef50_P34714 Cluster: SPARC precursor; n=3; Caenorhabditis|Rep:
SPARC precursor - Caenorhabditis elegans
Length = 264
Score = 52.4 bits (120), Expect = 1e-05
Identities = 23/63 (36%), Positives = 33/63 (52%)
Frame = +2
Query: 536 HETWQSDCEVYRXRCLCLDNSDQCRGPXYHHVXIEYYXTCRXMPXCTESXMSDFPPPHAG 715
++T+ S C++YR RCLC S +C V +EY C+ + CTE M+ FP A
Sbjct: 96 NQTFTSLCDLYRERCLCKRKSKECSKAFNAKVHLEYLGECKKLDECTEEHMAQFPERMAD 155
Query: 716 LXF 724
F
Sbjct: 156 WLF 158
Score = 33.1 bits (72), Expect = 9.7
Identities = 13/37 (35%), Positives = 18/37 (48%), Gaps = 1/37 (2%)
Frame = +3
Query: 426 CSAGRVCEINEHGDAMCNCIXDCP-YETDSRRXVCTN 533
C G+ C + + G+ C CI CP + D VC N
Sbjct: 58 CGWGKECVVGKKGEPTCECISKCPELDGDPMDKVCAN 94
>UniRef50_Q6PVV9 Cluster: SPARC; n=1; Ciona intestinalis|Rep: SPARC
- Ciona intestinalis (Transparent sea squirt)
Length = 366
Score = 41.5 bits (93), Expect = 0.028
Identities = 15/55 (27%), Positives = 31/55 (56%)
Frame = +2
Query: 536 HETWQSDCEVYRXRCLCLDNSDQCRGPXYHHVXIEYYXTCRXMPXCTESXMSDFP 700
+ T+ S+CE++R +C+ N + H+ ++YY C+ + C E +S++P
Sbjct: 175 NNTYTSECELWRTKCIMKQN----KAKGVQHLRLDYYGDCKEIQPCGEHELSEYP 225
>UniRef50_P09486 Cluster: SPARC precursor; n=15; Vertebrata|Rep:
SPARC precursor - Homo sapiens (Human)
Length = 303
Score = 36.3 bits (80), Expect = 1.0
Identities = 16/42 (38%), Positives = 21/42 (50%), Gaps = 2/42 (4%)
Frame = +3
Query: 423 HCSAGRVCEINEHGDAMCNC--IXDCPYETDSRRXVCTNFTK 542
HC G+VCE++E+ MC C CP VC+N K
Sbjct: 76 HCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNK 117
>UniRef50_Q1WIX6 Cluster: Follistatin-related protein; n=2;
Haemaphysalis longicornis|Rep: Follistatin-related
protein - Haemaphysalis longicornis (Bush tick)
Length = 289
Score = 35.5 bits (78), Expect = 1.8
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = +3
Query: 420 VHCSAGRVCEINEHGDAMCNCIXDCP 497
V C GRVC+I ++G A C C+ CP
Sbjct: 37 VVCRPGRVCQILDNGLASCQCVQHCP 62
>UniRef50_P07214 Cluster: SPARC precursor; n=24; Euteleostomi|Rep:
SPARC precursor - Mus musculus (Mouse)
Length = 302
Score = 35.5 bits (78), Expect = 1.8
Identities = 16/42 (38%), Positives = 20/42 (47%), Gaps = 2/42 (4%)
Frame = +3
Query: 423 HCSAGRVCEINEHGDAMCNC--IXDCPYETDSRRXVCTNFTK 542
HC G+VCE++E MC C CP VC+N K
Sbjct: 75 HCKHGKVCELDESNTPMCVCQDPTSCPAPIGEFEKVCSNDNK 116
>UniRef50_UPI000051A338 Cluster: PREDICTED: similar to agrin isoform
1; n=1; Apis mellifera|Rep: PREDICTED: similar to agrin
isoform 1 - Apis mellifera
Length = 2397
Score = 34.7 bits (76), Expect = 3.2
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = +3
Query: 423 HCSAGRVCEINEHGDAMCNCIXDCP 497
+CS G C ++E+G +C C DCP
Sbjct: 479 YCSWGATCVVSENGKPLCQCPTDCP 503
>UniRef50_Q3WGV9 Cluster: Regulatory protein, TetR; n=1; Frankia sp.
EAN1pec|Rep: Regulatory protein, TetR - Frankia sp.
EAN1pec
Length = 221
Score = 34.7 bits (76), Expect = 3.2
Identities = 20/68 (29%), Positives = 34/68 (50%), Gaps = 3/68 (4%)
Frame = -2
Query: 361 LVEKVVNSGYFCLMVSLLVQALRHSVLDVFSL---RDVPAAELLRRWTFSSRGADPAVPT 191
++E + + G+ + + + + R S L ++ L RD L+RW ++ GA PA PT
Sbjct: 17 VLELLESGGHDAVQMREVARRARVSPLTIYKLFPGRDELVVAALQRWLAANSGASPAPPT 76
Query: 190 PHTTSVGG 167
P T G
Sbjct: 77 PDETLYDG 84
>UniRef50_Q62356 Cluster: Follistatin-related protein 1 precursor;
n=11; Euteleostomi|Rep: Follistatin-related protein 1
precursor - Mus musculus (Mouse)
Length = 306
Score = 33.9 bits (74), Expect = 5.6
Identities = 15/36 (41%), Positives = 18/36 (50%)
Frame = +3
Query: 420 VHCSAGRVCEINEHGDAMCNCIXDCPYETDSRRXVC 527
V C AGR C + E G+ C CI C +R VC
Sbjct: 32 VFCGAGRECAVTEKGEPTCLCIEQC---KPHKRPVC 64
>UniRef50_Q12841 Cluster: Follistatin-related protein 1 precursor;
n=32; Euteleostomi|Rep: Follistatin-related protein 1
precursor - Homo sapiens (Human)
Length = 308
Score = 33.9 bits (74), Expect = 5.6
Identities = 15/36 (41%), Positives = 18/36 (50%)
Frame = +3
Query: 420 VHCSAGRVCEINEHGDAMCNCIXDCPYETDSRRXVC 527
V C AGR C + E G+ C CI C +R VC
Sbjct: 34 VFCGAGRECAVTEKGEPTCLCIEQC---KPHKRPVC 66
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 644,148,696
Number of Sequences: 1657284
Number of extensions: 11029596
Number of successful extensions: 25462
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 24318
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25449
length of database: 575,637,011
effective HSP length: 100
effective length of database: 409,908,611
effective search space used: 79932179145
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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