Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP08_F_F07
         (857 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   334   7e-93
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   330   1e-91
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   168   1e-42
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   108   9e-25
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom...    34   0.030
SPBC12C2.05c |||diacylglycerol binding protein Bzz1 |Schizosacch...    27   3.4  
SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   7.9  

>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  334 bits (822), Expect = 7e-93
 Identities = 152/229 (66%), Positives = 179/229 (78%)
 Frame = +2

Query: 50  GTXXQLFHPXXLXTGKXXXXXXXXRGHXTIGKXIVXLVLDRIRKLADQCTGLQGFLIFHX 229
           G    LFHP  + TGK        RGH T+GK ++  VL+RIR++AD C+GLQGFL+FH 
Sbjct: 85  GPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHS 144

Query: 230 XXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTXLEHSDC 409
                      LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH  L++SDC
Sbjct: 145 FGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDC 204

Query: 410 AFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLV 589
            FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSITASLRF G+LNVDL EFQTNLV
Sbjct: 205 TFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLNVDLNEFQTNLV 264

Query: 590 PYPRIHFPLVTYAPVISXEKAYHEQLSVAEITNACFEPANQMVXCXPRS 736
           PYPRIHFPLVTY+P++S  KA+HE  SV EITN CFEP NQMV C PR+
Sbjct: 265 PYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRT 313


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  330 bits (811), Expect = 1e-91
 Identities = 151/229 (65%), Positives = 178/229 (77%)
 Frame = +2

Query: 50  GTXXQLFHPXXLXTGKXXXXXXXXRGHXTIGKXIVXLVLDRIRKLADQCTGLQGFLIFHX 229
           G    LFHP  L TGK        RGH T+GK +V  V D+IR++AD C+GLQGFL+FH 
Sbjct: 81  GPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHS 140

Query: 230 XXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTXLEHSDC 409
                      LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH  L+ +DC
Sbjct: 141 FGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADC 200

Query: 410 AFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLV 589
            FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASLRF+G+LNVDL EFQTNLV
Sbjct: 201 TFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLNVDLAEFQTNLV 260

Query: 590 PYPRIHFPLVTYAPVISXEKAYHEQLSVAEITNACFEPANQMVXCXPRS 736
           PYPRIHFPLVTYAP++S  KA+HE  SV EITN CFEP NQMV C PR+
Sbjct: 261 PYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRA 309


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  168 bits (408), Expect = 1e-42
 Identities = 81/228 (35%), Positives = 124/228 (54%)
 Frame = +2

Query: 50  GTXXQLFHPXXLXTGKXXXXXXXXRGHXTIGKXIVXLVLDRIRKLADQCTGLQGFLIFHX 229
           G    LF P  +  G+        +GH T G  +   VLD +R+ A+ C  LQGF + H 
Sbjct: 79  GKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHS 138

Query: 230 XXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTXLEHSDC 409
                      LL+ ++  +Y  +    F++ PAP+ S  VVEPYN+ L+ H  +E+SD 
Sbjct: 139 LGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDE 198

Query: 410 AFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLV 589
            F +DNEA+  I    L I+ P+Y +LN L+  +++ +T S RF G LN DL +   N+V
Sbjct: 199 TFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNMV 258

Query: 590 PYPRIHFPLVTYAPVISXEKAYHEQLSVAEITNACFEPANQMVXCXPR 733
           P+PR+HF +V +AP+ +   +  + +SV E+T   F+  N MV   PR
Sbjct: 259 PFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPR 306


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  108 bits (260), Expect = 9e-25
 Identities = 61/194 (31%), Positives = 97/194 (50%), Gaps = 4/194 (2%)
 Frame = +2

Query: 161 VLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ- 337
           ++D I + AD    L+GF + H             L+ERL+  Y KK    ++++P  Q 
Sbjct: 118 IMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQS 177

Query: 338 VSTAVVEPYNSILTTHTXLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVS 517
           VS  VV+PYNS+L       ++D   ++DN A+  I    L  + PT+   N+L+  ++S
Sbjct: 178 VSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMS 237

Query: 518 SITASLRFDGALNVDLTEFQTNLVPYPRIHFPLVTYAPVIS---XEKAYHEQLSVAEITN 688
           + T +LR+ G +N DL     +L+P PR HF L +Y P  +    E     + +V ++  
Sbjct: 238 ASTTTLRYPGYMNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMR 297

Query: 689 ACFEPANQMVXCXP 730
               P NQMV   P
Sbjct: 298 RLLLPKNQMVSVNP 311


>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
           Msp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 903

 Score = 33.9 bits (74), Expect = 0.030
 Identities = 18/54 (33%), Positives = 30/54 (55%)
 Frame = -2

Query: 592 RHQVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 431
           R  +  E  + H  ++ + Q+SSD  ++ +D T  VG+G AL   I + D +DG
Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216


>SPBC12C2.05c |||diacylglycerol binding protein Bzz1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 642

 Score = 27.1 bits (57), Expect = 3.4
 Identities = 30/111 (27%), Positives = 46/111 (41%), Gaps = 8/111 (7%)
 Frame = -2

Query: 625 VRDQWEVDTGVRHQVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAA 446
           V D   VDT V      +       +A ES R++   D  S    Q  +      E T A
Sbjct: 454 VADDDAVDTSVTATDDFD-ASASSSNAYESYRNTYTDDMDSSSIYQTSLSNVKTEETTPA 512

Query: 445 DV---IDGFIV-----DHESTVRVLKXCVGGEDRVVGLDDGSGNLRRGVDG 317
           +    +DG ++     +HE  +   +   G E  ++  DDGSG +R  +DG
Sbjct: 513 EPASKVDGVVLYDFTGEHEGVITASE---GQEFTLLEPDDGSGWVRVKIDG 560


>SPBC428.10 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 751

 Score = 25.8 bits (54), Expect = 7.9
 Identities = 16/47 (34%), Positives = 26/47 (55%)
 Frame = +3

Query: 564 SPSSRLTWCLTPVSTSHWSRTRQSSLXRRPTMNSFPSPRSQTHASSP 704
           SPS  LT  L   S++  S  + + + R+ T +S  SP+ Q+  S+P
Sbjct: 619 SPSKMLT-TLRNNSSTFPSLRKNAMIARKSTADSLSSPKRQSVPSTP 664


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,922,647
Number of Sequences: 5004
Number of extensions: 54764
Number of successful extensions: 166
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)