Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP08_F_D21
         (866 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa...    93   5e-20
SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz...    63   6e-11
SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac...    32   0.092
SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyce...    30   0.37 

>SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 210

 Score = 93.1 bits (221), Expect = 5e-20
 Identities = 54/186 (29%), Positives = 94/186 (50%), Gaps = 4/186 (2%)
 Frame = +3

Query: 189 LSKSVELTFELPDSAEECFY-QTMDKDTAASLEYQVISGGQYDVSVKIEAPNRQIIYQQE 365
           L+ ++ELTF+L +  ++C+Y  +          Y V SGG +DV   I+AP+++ +   +
Sbjct: 17  LASAIELTFKLENQEKQCYYLDSFHTGEKTHFTYAVQSGGSFDVDYMIKAPSQKTVALGK 76

Query: 366 KMXYDSHQFTALASGDYKVCFSNEFSTFSHKLVYMELNVGPEQPLPGV-GDXATVLTQ-- 536
           K       FT    G+Y+ CF N  STF+ K+V ME+ +  E  LP +  D A    +  
Sbjct: 77  KRRQADVFFTLEEKGEYEFCFDNHMSTFTDKIVTMEITMENELSLPALTRDEAKDYKKDS 136

Query: 537 LETSAEEIHSALNRIIDHQTHHXLREAXGRKRAEDLXXXVFWWSMGXTXAIVCVAFTPVM 716
           ++++  EI +AL+ I   Q +   RE       +     +FW+S+  +  +V ++   V 
Sbjct: 137 MQSTVLEISTALSEIDRVQNYFKTREHRNYSTVKSTQARIFWFSLAESIMVVALSALQVF 196

Query: 717 ILXNFF 734
           I+  FF
Sbjct: 197 IVKTFF 202


>SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 199

 Score = 62.9 bits (146), Expect = 6e-11
 Identities = 43/169 (25%), Positives = 67/169 (39%)
 Frame = +3

Query: 237 ECFYQTMDKDTAASLEYQVISGGQYDVSVKIEAPNRQIIYQQEKMXYDSHQFTALASGDY 416
           ECFY+ +  +   S+ YQ   GG   VS+ I  P  QI++Q+       + FT    G Y
Sbjct: 32  ECFYENLRNNDQMSVTYQTNVGGDQLVSMSIYNPAGQIMHQEVPNSMAQYSFTVKNPGKY 91

Query: 417 KVCFSNEFSTFSHKLVYMELNVGPEQPLPGVGDXATVLTQLETSAEEIHSALNRIIDHQT 596
             CF N+      K V   ++      +    +       L     ++H  ++++   Q 
Sbjct: 92  MYCFYNDALDGESKEVLFNVH----GVIYISDEDLDANNPLLGKVRQLHDTISKVKHEQE 147

Query: 597 HHXLREAXGRKRAEDLXXXVFWWSMGXTXAIVCVAFTPVMILXNFFXDK 743
           +   RE   R  AE     V WWS+  T  +V V    +  L   F  K
Sbjct: 148 YFVARERIHRNTAESTNDRVKWWSILQTVILVSVCVFQIFYLKRLFEVK 196


>SPBC16E9.09c |||COPII vesicle coat component
           Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 215

 Score = 32.3 bits (70), Expect = 0.092
 Identities = 22/134 (16%), Positives = 52/134 (38%), Gaps = 2/134 (1%)
 Frame = +3

Query: 339 NRQIIYQQEKMXYDSHQFTALASGDYKVCF--SNEFSTFSHKLVYMELNVGPEQPLPGVG 512
           N  +IY+ +    ++ +FT    G +++C+  +++ + FS     + +++  +     + 
Sbjct: 76  NNHLIYKNDLRSNEAVRFTTTNEGTHRICYLSTSDGAWFSSTKTRLHVDLASDDKYNLIA 135

Query: 513 DXATVLTQLETSAEEIHSALNRIIDHQTHHXLREAXGRKRAEDLXXXVFWWSMGXTXAIV 692
                   L      +++ L  I+  Q     RE   R  +E     V  W++     ++
Sbjct: 136 SSEDEAKDLTDRVAALNTRLESILGEQMLQRSREMTFRDTSESANSRVVRWTIVQIVVLL 195

Query: 693 CVAFTPVMILXNFF 734
                 +  L  FF
Sbjct: 196 VTCIWQLSHLQRFF 209


>SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 818

 Score = 30.3 bits (65), Expect = 0.37
 Identities = 28/100 (28%), Positives = 43/100 (43%), Gaps = 7/100 (7%)
 Frame = -2

Query: 529 RTVAXSPTPGRGCSGPTFSSIYTSLCENVLNSLLKHTL*SPDARAVNWCESYXIFSC*YI 350
           R+   SPT       PT +    +  E V N+L K+   S   R     + Y   S  Y+
Sbjct: 460 RSTTTSPTSAVASPAPTENEDSNAAIERVANTLRKNPTISRRTRRAGTMDRYATASSDYM 519

Query: 349 ICLLGA--SIFTLTSYCPPE--ITW---YSNEAAVSLSIV 251
              LG+  ++ TL+    P+   TW   + N + +S SIV
Sbjct: 520 ESNLGSLPNLSTLSLQSGPDSTATWHPEFPNSSGLSASIV 559


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,825,570
Number of Sequences: 5004
Number of extensions: 52088
Number of successful extensions: 115
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 112
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 432473040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)