Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP08_F_B07
(859 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|... 105 1e-23
SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2||... 91 2e-19
SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1||... 77 3e-15
SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|ch... 70 4e-13
SPAC4C5.02c |ryh1|hos1|GTPase Ryh1|Schizosaccharomyces pombe|chr... 63 4e-11
SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr 2||... 56 6e-09
SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1||... 53 5e-08
SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||... 52 8e-08
SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr... 52 1e-07
SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|ch... 35 0.017
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 30 0.48
SPAC27D7.08c |||DUF890 family protein|Schizosaccharomyces pombe|... 26 6.0
SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3 B... 26 6.0
>SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 200
Score = 105 bits (251), Expect = 1e-23
Identities = 49/99 (49%), Positives = 74/99 (74%), Gaps = 1/99 (1%)
Frame = +3
Query: 375 LCLSTNVTXEKSFENIKNWIRNIEXNASADVEKMILGNKCDLDSQRQVSKERGEQLAIEY 554
+ L +VT +KSF+N++ W N+E +AS +V K+++GNKCD + QRQVS E+G+ LA E
Sbjct: 85 ILLLYDVTDKKSFDNVRTWFSNVEQHASENVYKILIGNKCDCEDQRQVSFEQGQALADEL 144
Query: 555 QIKFVETSAKDSLNVEYAFYTLARDI-KAKMEKKQEASN 668
+KF+E SAK ++NV+ AF+TLAR+I K K++ + E SN
Sbjct: 145 GVKFLEASAKTNVNVDEAFFTLAREIKKQKIDAENEFSN 183
Score = 100 bits (239), Expect = 3e-22
Identities = 48/78 (61%), Positives = 57/78 (73%)
Frame = +2
Query: 158 LLIGASGVGXXSILFRFSXAAFXXSFXSXIGXXFKIRTXALXGKKVKLQXWXXAGQARFR 337
LLIG SGVG +L RFS +F SF + IG FKIRT L GK++KLQ W AGQ RFR
Sbjct: 13 LLIGDSGVGKSCLLLRFSEDSFTPSFITTIGIDFKIRTIELDGKRIKLQIWDTAGQERFR 72
Query: 338 TITTAYXRGSMGIMLVYE 391
TITTAY RG+MGI+L+Y+
Sbjct: 73 TITTAYYRGAMGILLLYD 90
>SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 203
Score = 91.1 bits (216), Expect = 2e-19
Identities = 44/78 (56%), Positives = 54/78 (69%)
Frame = +2
Query: 158 LLIGASGVGXXSILFRFSXAAFXXSFXSXIGXXFKIRTXALXGKKVKLQXWXXAGQARFR 337
LLIG SGVG +L RF+ + S+ S IG FKIRT L GK VKLQ W AGQ RFR
Sbjct: 12 LLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTFELEGKTVKLQIWDTAGQERFR 71
Query: 338 TITTAYXRGSMGIMLVYE 391
TIT++Y RG+ GI++VY+
Sbjct: 72 TITSSYYRGAHGIIIVYD 89
Score = 77.4 bits (182), Expect = 2e-15
Identities = 37/93 (39%), Positives = 57/93 (61%)
Frame = +3
Query: 390 NVTXEKSFENIKNWIRNIEXNASADVEKMILGNKCDLDSQRQVSKERGEQLAIEYQIKFV 569
+VT + SF N+K W++ I+ A V ++++GNK D+ ++ V ++ A I F+
Sbjct: 89 DVTDQDSFNNVKQWLQEIDRYAVEGVNRLLVGNKSDMVDKKVVEYSVAKEFADSLNIPFL 148
Query: 570 ETSAKDSLNVEYAFYTLARDIKAKMEKKQEASN 668
ETSAKDS NVE AF T++R IK +M AS+
Sbjct: 149 ETSAKDSTNVEQAFLTMSRQIKERMGNNTFASS 181
>SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr
1|||Manual
Length = 214
Score = 77.0 bits (181), Expect = 3e-15
Identities = 36/78 (46%), Positives = 48/78 (61%)
Frame = +2
Query: 158 LLIGASGVGXXSILFRFSXAAFXXSFXSXIGXXFKIRTXALXGKKVKLQXWXXAGQARFR 337
+LIG SGVG ++L RF+ F S IG F R L KK+K Q W AGQ R+R
Sbjct: 14 VLIGDSGVGKSNLLMRFTRNEFNIESKSTIGVEFATRNIVLDNKKIKAQIWDTAGQERYR 73
Query: 338 TITTAYXRGSMGIMLVYE 391
IT+AY RG++G ++VY+
Sbjct: 74 AITSAYYRGAVGALIVYD 91
Score = 70.5 bits (165), Expect = 3e-13
Identities = 31/81 (38%), Positives = 51/81 (62%)
Frame = +3
Query: 390 NVTXEKSFENIKNWIRNIEXNASADVEKMILGNKCDLDSQRQVSKERGEQLAIEYQIKFV 569
++T + SF+N+ W++ + +A +++ M++GNK DL R VS E + A E + F+
Sbjct: 91 DITKQSSFDNVGRWLKELREHADSNIVIMLVGNKTDLLHLRAVSTEEAQAFAAENNLSFI 150
Query: 570 ETSAKDSLNVEYAFYTLARDI 632
ETSA D+ NVE AF T+ +I
Sbjct: 151 ETSAMDASNVEEAFQTVLTEI 171
>SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 219
Score = 70.1 bits (164), Expect = 4e-13
Identities = 39/91 (42%), Positives = 54/91 (59%), Gaps = 1/91 (1%)
Frame = +3
Query: 390 NVTXEKSFENIKNWIRNIEXNASADVEKMIL-GNKCDLDSQRQVSKERGEQLAIEYQIKF 566
N+T SF+ I + + I D ++L NKCDL+++R VS+ GEQLA +
Sbjct: 88 NITSRSSFDEISTFYQQILRVKDKDTFPVVLVANKCDLEAERVVSRAEGEQLAKSMHCLY 147
Query: 567 VETSAKDSLNVEYAFYTLARDIKAKMEKKQE 659
VETSAK LNVE AFY+L R I+ + K +E
Sbjct: 148 VETSAKLRLNVEEAFYSLVRTIR-RYNKSEE 177
Score = 30.7 bits (66), Expect = 0.28
Identities = 17/77 (22%), Positives = 33/77 (42%)
Frame = +2
Query: 158 LLIGASGVGXXSILFRFSXAAFXXSFXSXIGXXFKIRTXALXGKKVKLQXWXXAGQARFR 337
+++G GVG ++ + + F + I ++ + + G+ L AGQ +
Sbjct: 12 VVVGDGGVGKSALTIQLIQSHFVDEYDPTIEDSYR-KKCEIDGEGALLDVLDTAGQEEYS 70
Query: 338 TITTAYXRGSMGIMLVY 388
+ Y R G +LVY
Sbjct: 71 AMREQYMRTGEGFLLVY 87
>SPAC4C5.02c |ryh1|hos1|GTPase Ryh1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 201
Score = 63.3 bits (147), Expect = 4e-11
Identities = 31/79 (39%), Positives = 48/79 (60%)
Frame = +3
Query: 390 NVTXEKSFENIKNWIRNIEXNASADVEKMILGNKCDLDSQRQVSKERGEQLAIEYQIKFV 569
++T SF N + WI ++ DV +++GNK DL +RQV++E GE+ A E +I +
Sbjct: 92 DITNHNSFVNTEKWIEDVRAERGDDVIIVLVGNKTDLADKRQVTQEEGEKKAKELKIMHM 151
Query: 570 ETSAKDSLNVEYAFYTLAR 626
ETSAK NV+ F +A+
Sbjct: 152 ETSAKAGHNVKLLFRKIAQ 170
Score = 57.2 bits (132), Expect = 3e-09
Identities = 27/78 (34%), Positives = 43/78 (55%)
Frame = +2
Query: 158 LLIGASGVGXXSILFRFSXAAFXXSFXSXIGXXFKIRTXALXGKKVKLQXWXXAGQARFR 337
+ +G VG S++ RF F ++ + IG F +T L + V+LQ W AGQ RFR
Sbjct: 15 VFLGEQSVGKTSLITRFMYDQFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFR 74
Query: 338 TITTAYXRGSMGIMLVYE 391
++ +Y R S ++VY+
Sbjct: 75 SLIPSYIRDSSVAIIVYD 92
>SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr
2|||Manual
Length = 205
Score = 56.0 bits (129), Expect = 6e-09
Identities = 27/78 (34%), Positives = 44/78 (56%)
Frame = +2
Query: 158 LLIGASGVGXXSILFRFSXAAFXXSFXSXIGXXFKIRTXALXGKKVKLQXWXXAGQARFR 337
+++G SGVG SI+ ++ F + + IG F + + K V LQ W AGQ RF+
Sbjct: 12 IILGESGVGKTSIMNQYVNRKFSKDYKATIGADFLTKEVLVDDKVVTLQLWDTAGQERFQ 71
Query: 338 TITTAYXRGSMGIMLVYE 391
++ A+ RG+ +LVY+
Sbjct: 72 SLGVAFYRGADCCVLVYD 89
Score = 47.6 bits (108), Expect = 2e-06
Identities = 32/99 (32%), Positives = 52/99 (52%), Gaps = 6/99 (6%)
Frame = +3
Query: 390 NVTXEKSFENIKNWIRNIEXNASADVEK----MILGNKCDLDSQ-RQVSKERGEQLA-IE 551
+V KSFE + +W AS + ++LGNK D++ Q R VSK +
Sbjct: 89 DVNNSKSFETLDSWRDEFLIQASPSNPETFPFILLGNKVDVEEQKRMVSKSKALAFCQAR 148
Query: 552 YQIKFVETSAKDSLNVEYAFYTLARDIKAKMEKKQEASN 668
+I + ETSAK+++NV+ AF T+A+ M+ A++
Sbjct: 149 GEIPYFETSAKEAINVQEAFETVAKLALENMDSDDIAAD 187
>SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 234
Score = 53.2 bits (122), Expect = 5e-08
Identities = 29/87 (33%), Positives = 47/87 (54%), Gaps = 1/87 (1%)
Frame = +3
Query: 390 NVTXEKSFENIKNWIRNIEXNASADVEKMILGNKCDLDSQRQVSKERGEQLAIEYQIKFV 569
+VT + SFE + +W+ +I A + + ++ G+K DL +QRQVS E + E I
Sbjct: 94 DVTNKDSFEELSSWLSDIRAMAPSTICVVLAGSKSDLQNQRQVSTEEAAEFCSEKHISSA 153
Query: 570 -ETSAKDSLNVEYAFYTLARDIKAKME 647
ETS+ NVE F ++ I ++E
Sbjct: 154 HETSSYTGSNVEECFLSVVSTIITRIE 180
Score = 52.4 bits (120), Expect = 8e-08
Identities = 28/82 (34%), Positives = 42/82 (51%), Gaps = 4/82 (4%)
Frame = +2
Query: 158 LLIGASGVGXXSILFRFSXAAFXXSFXSXIGXXFKIRTXALX----GKKVKLQXWXXAGQ 325
+L G SG G +L RF + +G F R ++ K++KLQ W AGQ
Sbjct: 13 VLAGPSGTGKSCLLQRFVKNQWDDQVSHTVGIDFASRIISVGMGNQQKRIKLQIWDTAGQ 72
Query: 326 ARFRTITTAYXRGSMGIMLVYE 391
+FR++ Y RG+ G +LVY+
Sbjct: 73 EKFRSVARNYYRGAAGAVLVYD 94
>SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr
1|||Manual
Length = 211
Score = 52.4 bits (120), Expect = 8e-08
Identities = 28/82 (34%), Positives = 43/82 (52%), Gaps = 1/82 (1%)
Frame = +3
Query: 390 NVTXEKSFENIKNWIRNIEXNASADVEKMILGNKCDLDSQRQ-VSKERGEQLAIEYQIKF 566
++T S E K+WI+ ++ A + + GNK DL +R+ V K E A E + F
Sbjct: 96 DITQAASLEKAKSWIKELQRQAPEGIVIALAGNKLDLAQERRAVEKADAEAYAAEANLLF 155
Query: 567 VETSAKDSLNVEYAFYTLARDI 632
ETSAK + NV F +A+ +
Sbjct: 156 FETSAKTAENVNELFTAIAKKL 177
Score = 49.6 bits (113), Expect = 6e-07
Identities = 26/84 (30%), Positives = 43/84 (51%), Gaps = 1/84 (1%)
Frame = +2
Query: 143 VSSNFLLIGASGVGXXSILFRFSXAAFXXSFXSXIGXXFKIRTXALX-GKKVKLQXWXXA 319
++ +L+G S VG S++ RF F S IG F +T + VKL+ W A
Sbjct: 13 INQKLVLLGDSAVGKSSLVLRFVKDQFDDYRESTIGAAFLTQTLPIDENTSVKLEIWDTA 72
Query: 320 GQARFRTITTAYXRGSMGIMLVYE 391
GQ R++++ Y R + ++VY+
Sbjct: 73 GQERYKSLAPMYYRNANCAIVVYD 96
>SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr
1|||Manual
Length = 208
Score = 52.0 bits (119), Expect = 1e-07
Identities = 25/77 (32%), Positives = 42/77 (54%)
Frame = +2
Query: 158 LLIGASGVGXXSILFRFSXAAFXXSFXSXIGXXFKIRTXALXGKKVKLQXWXXAGQARFR 337
+++G SGVG ++ +F F + + IG F + + K V LQ W AGQ RF+
Sbjct: 12 VILGDSGVGKTCLMNQFVNQKFSREYKATIGADFLTKDVVVDDKLVTLQLWDTAGQERFQ 71
Query: 338 TITTAYXRGSMGIMLVY 388
++ A+ RG+ ++VY
Sbjct: 72 SLGMAFYRGADCCVIVY 88
Score = 37.9 bits (84), Expect = 0.002
Identities = 28/86 (32%), Positives = 45/86 (52%), Gaps = 7/86 (8%)
Frame = +3
Query: 390 NVTXEKSFENIKNWIRNIEXNASAD---VEKMILGNKCDLD-SQRQVSKERG-EQLAIEY 554
NV KSF++++NW + S D +I+GN+ D D S+R VS R + ++
Sbjct: 89 NVNNSKSFDSVENWRQEFLYQTSQDECAFPFIIVGNQIDKDASKRAVSLHRALDYCKSKH 148
Query: 555 QIKFV--ETSAKDSLNVEYAFYTLAR 626
+ E SAK++ NV F T++R
Sbjct: 149 GSNMIHFEASAKENTNVTDLFETVSR 174
>SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 216
Score = 34.7 bits (76), Expect = 0.017
Identities = 21/81 (25%), Positives = 44/81 (54%)
Frame = +3
Query: 390 NVTXEKSFENIKNWIRNIEXNASADVEKMILGNKCDLDSQRQVSKERGEQLAIEYQIKFV 569
+VT +++N+ +W R++ ++ ++ GNK D+ +R+V K + + +++
Sbjct: 90 DVTSRITYKNVPHWWRDL-VRVCENIPIVLCGNKVDV-KERKV-KAKAITFHRKKNLQYY 146
Query: 570 ETSAKDSLNVEYAFYTLARDI 632
+ SAK + N E F LAR +
Sbjct: 147 DISAKSNYNFEKPFLWLARKL 167
>SPBC21D10.06c |map4||cell agglutination protein
Map4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 948
Score = 29.9 bits (64), Expect = 0.48
Identities = 16/37 (43%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
Frame = -1
Query: 667 LLASCFFSIFAFMSLAKV*NAYSTF-NESFADVSTNF 560
+L S FFS ++LA + YS F N SF D T+F
Sbjct: 6 ILLSLFFSFERLLTLANANSLYSPFNNSSFVDSDTSF 42
>SPAC27D7.08c |||DUF890 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 385
Score = 26.2 bits (55), Expect = 6.0
Identities = 13/31 (41%), Positives = 20/31 (64%), Gaps = 1/31 (3%)
Frame = -2
Query: 669 DYSLPVSFPFSLLCPWLKCK-MHIQHLTSLL 580
DYSL V FP + LCP + + +I+++ LL
Sbjct: 35 DYSLRVEFPENRLCPMVPNRATYIRYIHDLL 65
>SPCC1919.15 |brl1|SPCC790.01, rfp2|ubiquitin-protein ligase E3
Brl1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 692
Score = 26.2 bits (55), Expect = 6.0
Identities = 21/80 (26%), Positives = 36/80 (45%), Gaps = 3/80 (3%)
Frame = +3
Query: 375 LCLSTNVTXEKSFENIKNWIRNIEXNASADVEKMILGNKC---DLDSQRQVSKERGEQLA 545
LC+ + K+ E E +AS+ +EK+I N C +L S + +L
Sbjct: 406 LCIWDRIERSKTGEMNNVLDEKEEISASSALEKLIKNNSCLEAELPSMYAAFDQSQSRLL 465
Query: 546 IEYQIKFVETSAKDSLNVEY 605
+Y+ +ET K +L + Y
Sbjct: 466 KKYE--ELETKEKKALEMHY 483
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,166,445
Number of Sequences: 5004
Number of extensions: 32369
Number of successful extensions: 91
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 81
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 91
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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