BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP08_F_A17
(940 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 33 0.058
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 31 0.23
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.31
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 30 0.54
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 30 0.54
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 28 2.2
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 27 5.0
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 27 5.0
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 27 5.0
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 27 5.0
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 26 6.7
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 6.7
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 26 8.8
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 33.1 bits (72), Expect = 0.058
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = -3
Query: 215 PPPPXXXXPPPPPPPP 168
PP P PPPPPPPP
Sbjct: 753 PPAPIMGGPPPPPPPP 768
Score = 31.9 bits (69), Expect = 0.13
Identities = 11/19 (57%), Positives = 11/19 (57%)
Frame = -1
Query: 400 PPPPXXVGLXXXPPPPPXP 344
PPPP G PPPPP P
Sbjct: 765 PPPPGVAGAGPPPPPPPPP 783
Score = 31.1 bits (67), Expect = 0.23
Identities = 15/53 (28%), Positives = 16/53 (30%)
Frame = +2
Query: 782 NPXXPPPXXXXXXXXXXXXXXXXNXPPXKXXPXXPPPXXAXXQNPPPXXPPXP 940
+P PPP P P PPP PPP PP P
Sbjct: 731 SPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 31.1 bits (67), Expect = 0.23
Identities = 12/18 (66%), Positives = 12/18 (66%), Gaps = 2/18 (11%)
Frame = -3
Query: 215 PPPPXXXX--PPPPPPPP 168
PPPP PPPPPPPP
Sbjct: 765 PPPPGVAGAGPPPPPPPP 782
Score = 30.7 bits (66), Expect = 0.31
Identities = 11/21 (52%), Positives = 12/21 (57%)
Frame = -1
Query: 400 PPPPXXVGLXXXPPPPPXPXP 338
PPPP G+ PPPP P P
Sbjct: 762 PPPPPPPGVAGAGPPPPPPPP 782
Score = 30.3 bits (65), Expect = 0.41
Identities = 15/42 (35%), Positives = 15/42 (35%)
Frame = +3
Query: 696 PPPPPXXRXXXXXXXHQTXTPXPAPXPTXTPXXPPPPXXPRG 821
PPPPP P P P P PPPP P G
Sbjct: 732 PPPPPPA----VIVPTPAPAPIPVPPPAPIMGGPPPPPPPPG 769
Score = 29.5 bits (63), Expect = 0.72
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -3
Query: 215 PPPPXXXXPPPPPPPP 168
PPP PPPPPPP
Sbjct: 752 PPPAPIMGGPPPPPPP 767
Score = 27.5 bits (58), Expect = 2.9
Identities = 20/61 (32%), Positives = 20/61 (32%)
Frame = +1
Query: 418 KRPPPPRXTDXXXXXXXLPRAHPXXPXAAGRGGAXXXPPPPRGQEXXXXRPGGPRPXXXE 597
K PPPP P P P A GG PPPP G P P P
Sbjct: 730 KSPPPPPPA--VIVPTPAPAPIPVPPPAPIMGGPPPPPPPP-GVAGAGPPPPPPPPPAVS 786
Query: 598 A 600
A
Sbjct: 787 A 787
Score = 27.5 bits (58), Expect = 2.9
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = -3
Query: 215 PPPPXXXXPPPPPPPPXXXP 156
PPPP PPPPP P
Sbjct: 764 PPPPPGVAGAGPPPPPPPPP 783
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 31.1 bits (67), Expect = 0.23
Identities = 12/18 (66%), Positives = 12/18 (66%), Gaps = 2/18 (11%)
Frame = -3
Query: 215 PPPPXXXXPP--PPPPPP 168
PPPP PP PPPPPP
Sbjct: 12 PPPPPGFEPPSQPPPPPP 29
Score = 30.3 bits (65), Expect = 0.41
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = +2
Query: 857 PPXKXXPXXPPPXXAXXQNPPPXXPP 934
PP P PPP PPP PP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
Score = 29.5 bits (63), Expect = 0.72
Identities = 12/26 (46%), Positives = 12/26 (46%)
Frame = +3
Query: 756 PXPAPXPTXTPXXPPPPXXPRGQXKK 833
P P P P P PPP P G KK
Sbjct: 10 PPPPPPPGFEPPSQPPPPPPPGYVKK 35
Score = 28.3 bits (60), Expect = 1.7
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = -3
Query: 191 PPPPPPPPXXXP 156
PPPPPPPP P
Sbjct: 9 PPPPPPPPGFEP 20
Score = 28.3 bits (60), Expect = 1.7
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = -3
Query: 215 PPPPXXXXPPPPPPPPXXXP 156
PPPP PP PPP P
Sbjct: 11 PPPPPPGFEPPSQPPPPPPP 30
Score = 27.9 bits (59), Expect = 2.2
Identities = 12/29 (41%), Positives = 13/29 (44%)
Frame = -1
Query: 400 PPPPXXVGLXXXPPPPPXPXPXXERGXXT 314
PPPP PPPPP P +R T
Sbjct: 12 PPPPPGFEPPSQPPPPPPPGYVKKRKNKT 40
Score = 27.5 bits (58), Expect = 2.9
Identities = 10/21 (47%), Positives = 10/21 (47%)
Frame = -1
Query: 400 PPPPXXVGLXXXPPPPPXPXP 338
PPPP G PPP P P
Sbjct: 10 PPPPPPPGFEPPSQPPPPPPP 30
Score = 26.2 bits (55), Expect = 6.7
Identities = 11/21 (52%), Positives = 11/21 (52%), Gaps = 3/21 (14%)
Frame = -3
Query: 209 PPXXXXPPPPPP---PPXXXP 156
PP PPPPPP PP P
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPP 25
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 30.7 bits (66), Expect = 0.31
Identities = 20/81 (24%), Positives = 24/81 (29%), Gaps = 1/81 (1%)
Frame = +1
Query: 391 GGGGXXSXGKRPPPPRXTDXXXXXXXLPRAHPXXPXAAGRGG-AXXXPPPPRGQEXXXXR 567
G G S PPPP ++ P+ P R + PPP
Sbjct: 328 GNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSN 387
Query: 568 PGGPRPXXXEAPXPXPPPXXN 630
P P P P PP N
Sbjct: 388 PPAPPPAIPGRSAPALPPLGN 408
Score = 27.5 bits (58), Expect = 2.9
Identities = 12/34 (35%), Positives = 13/34 (38%)
Frame = +1
Query: 529 PPPPRGQEXXXXRPGGPRPXXXEAPXPXPPPXXN 630
PPPP + P G P P PPP N
Sbjct: 313 PPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSN 346
Score = 26.2 bits (55), Expect = 6.7
Identities = 9/15 (60%), Positives = 9/15 (60%)
Frame = -3
Query: 215 PPPPXXXXPPPPPPP 171
P PP PPPPPP
Sbjct: 352 PLPPQGRSAPPPPPP 366
Score = 23.8 bits (49), Expect(2) = 0.83
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = -3
Query: 191 PPPPPPP 171
PPPPPPP
Sbjct: 311 PPPPPPP 317
Score = 23.8 bits (49), Expect(2) = 0.83
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = -3
Query: 188 PPPPPPP 168
PPPPPPP
Sbjct: 337 PPPPPPP 343
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 29.9 bits (64), Expect = 0.54
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -3
Query: 215 PPPPXXXXPPPPPPPP 168
PPPP PPPP PP
Sbjct: 1707 PPPPPMSVPPPPSAPP 1722
Score = 27.9 bits (59), Expect = 2.2
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = -3
Query: 215 PPPPXXXXPPPPPPPPXXXP 156
PPPP PPP PP P
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGP 1727
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 29.9 bits (64), Expect = 0.54
Identities = 25/81 (30%), Positives = 25/81 (30%), Gaps = 1/81 (1%)
Frame = -3
Query: 938 GXGXGGGXGFXGGRXXXVXXXXGVXX-GGXXXXGVVFFXLSPGXXXXGGXXGGXGGXGGG 762
G GG GF GG G GG G GG GG GG GGG
Sbjct: 184 GHNGGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGG 243
Query: 761 XXXXXXXXXXXXGXPXGGXGG 699
G P G GG
Sbjct: 244 PGGFGGGLGGFGGGPGGFGGG 264
Score = 29.1 bits (62), Expect = 0.95
Identities = 26/80 (32%), Positives = 26/80 (32%), Gaps = 2/80 (2%)
Frame = -3
Query: 932 GXGGGXGFXGGRXXXVXXXXGVXXGGXXXXGVVFFXLSPGXXXXG--GXXGGXGGXGGGX 759
G GGG G G G G F PG G G GG GG GGG
Sbjct: 192 GFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGL 251
Query: 758 XXXXXXXXXXXGXPXGGXGG 699
G P GG GG
Sbjct: 252 GGFGGGPGGFGGGP-GGHGG 270
Score = 28.7 bits (61), Expect = 1.2
Identities = 19/42 (45%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = -1
Query: 814 GXXGG-GGXXGVXVGXGAGXGVXVWXXGG-GGGXRXXGGGGG 695
G GG GG G G G G G GG GGG GGG G
Sbjct: 225 GFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPG 266
Score = 26.2 bits (55), Expect = 6.7
Identities = 17/41 (41%), Positives = 17/41 (41%), Gaps = 1/41 (2%)
Frame = -1
Query: 814 GXXGGGGXXGVXVGXGAGXGVXVWXXGG-GGGXRXXGGGGG 695
G G G G G G G G GG GGG GGG G
Sbjct: 212 GFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLG 252
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 27.9 bits (59), Expect = 2.2
Identities = 9/13 (69%), Positives = 9/13 (69%)
Frame = -3
Query: 206 PXXXXPPPPPPPP 168
P PPPPPPPP
Sbjct: 184 PSDYNPPPPPPPP 196
Score = 27.5 bits (58), Expect = 2.9
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = -3
Query: 209 PPXXXXPPPPPPPP 168
P PPPPPPPP
Sbjct: 184 PSDYNPPPPPPPPP 197
Score = 25.8 bits (54), Expect = 8.8
Identities = 11/30 (36%), Positives = 11/30 (36%)
Frame = +1
Query: 532 PPPRGQEXXXXRPGGPRPXXXEAPXPXPPP 621
PPP Q P P P P PPP
Sbjct: 167 PPPSFQPPSAAAPATSLPSDYNPPPPPPPP 196
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 26.6 bits (56), Expect = 5.0
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -3
Query: 191 PPPPPPPP 168
PPPPPPPP
Sbjct: 306 PPPPPPPP 313
Score = 26.6 bits (56), Expect = 5.0
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -3
Query: 191 PPPPPPPP 168
PPPPPPPP
Sbjct: 307 PPPPPPPP 314
Score = 23.0 bits (47), Expect(2) = 8.4
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = -1
Query: 364 PPPPPXPXP 338
PPPPP P P
Sbjct: 306 PPPPPPPPP 314
Score = 21.0 bits (42), Expect(2) = 8.4
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = -1
Query: 379 GLXXXPPPPP 350
GL PPPPP
Sbjct: 304 GLPPPPPPPP 313
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 26.6 bits (56), Expect = 5.0
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -3
Query: 191 PPPPPPPP 168
PPPPPPPP
Sbjct: 1095 PPPPPPPP 1102
>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 26.6 bits (56), Expect = 5.0
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -3
Query: 191 PPPPPPPP 168
PPPPPPPP
Sbjct: 370 PPPPPPPP 377
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 26.6 bits (56), Expect = 5.0
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -3
Query: 191 PPPPPPPP 168
PPPPPPPP
Sbjct: 945 PPPPPPPP 952
Score = 26.6 bits (56), Expect = 5.0
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -3
Query: 191 PPPPPPPP 168
PPPPPPPP
Sbjct: 946 PPPPPPPP 953
>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1334
Score = 26.2 bits (55), Expect = 6.7
Identities = 10/28 (35%), Positives = 12/28 (42%)
Frame = +2
Query: 857 PPXKXXPXXPPPXXAXXQNPPPXXPPXP 940
PP + P P P + PP PP P
Sbjct: 52 PPTRRLPRKPLPFRSTSLQPPSSQPPAP 79
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 26.2 bits (55), Expect = 6.7
Identities = 18/41 (43%), Positives = 18/41 (43%), Gaps = 1/41 (2%)
Frame = -1
Query: 814 GXXGG-GGXXGVXVGXGAGXGVXVWXXGGGGGXRXXGGGGG 695
G GG GG G G G G G GG GG R GG G
Sbjct: 13 GSRGGRGGFNGGRGGFGGGRGGA--RGGGRGGARGGRGGRG 51
Score = 26.2 bits (55), Expect = 6.7
Identities = 16/40 (40%), Positives = 16/40 (40%)
Frame = -1
Query: 817 RGXXGGGGXXGVXVGXGAGXGVXVWXXGGGGGXRXXGGGG 698
RG GGG G G G G G GG G R GG
Sbjct: 32 RGGARGGGRGGARGGRG-GRGGARGGRGGSSGGRGGAKGG 70
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 25.8 bits (54), Expect = 8.8
Identities = 15/40 (37%), Positives = 15/40 (37%)
Frame = -1
Query: 820 PRGXXGGGGXXGVXVGXGAGXGVXVWXXGGGGGXRXXGGG 701
P G G GG G G G G GGG GGG
Sbjct: 134 PAGRGGRGGFRGGRGGSRGGFGGNSRGGFGGGSRGGFGGG 173
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.313 0.151 0.533
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,885,779
Number of Sequences: 5004
Number of extensions: 30979
Number of successful extensions: 388
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 50
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 279
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 477327454
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.7 bits)
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