BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP07_F_P22
(913 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.69
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 2.1
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 27 3.7
SPAC22E12.04 |ccs1|pccs, pccs|metallochaperone Ccs1 |Schizosacch... 27 3.7
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 26 8.5
SPAC25B8.12c |||nucleotide-sugar phosphatase |Schizosaccharomyce... 26 8.5
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.5 bits (63), Expect = 0.69
Identities = 15/59 (25%), Positives = 16/59 (27%)
Frame = +2
Query: 716 PXPPQSPXMXPXXXLAXPXXXIXXPLPXTXPXXPQTSXXPXXPLGXPXPXXXPPPPXXP 892
P PP P P + P P P P P G P PP P
Sbjct: 418 PTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAP 476
Score = 27.9 bits (59), Expect = 2.1
Identities = 17/54 (31%), Positives = 17/54 (31%)
Frame = +2
Query: 722 PPQSPXMXPXXXLAXPXXXIXXPLPXTXPXXPQTSXXPXXPLGXPXPXXXPPPP 883
PP P P P I PLP P P P P P P P P
Sbjct: 436 PPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAP--PLPPAAPAPPPAPAPAPAAP 487
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 2.1
Identities = 13/33 (39%), Positives = 15/33 (45%), Gaps = 2/33 (6%)
Frame = +2
Query: 800 TXPXXPQTSXXP--XXPLGXPXPXXXPPPPXXP 892
T P PQ++ P P P P PPPP P
Sbjct: 1689 TPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAP 1721
>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
Srb9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1223
Score = 27.1 bits (57), Expect = 3.7
Identities = 17/61 (27%), Positives = 25/61 (40%)
Frame = +2
Query: 227 LPLDLDPALSFCTHLLYGYAGIQPDTYKLVSLNENLDIDRTHDNYRAITSLKAKYPGLTV 406
+P+ D T L GY + D L L+ +L I R HD Y + + Y +
Sbjct: 1125 MPVPNDEFKKISTILARGYLALDEDESYLPLLSIHLLISRNHDPYLMLNLILKHYLSMIY 1184
Query: 407 L 409
L
Sbjct: 1185 L 1185
>SPAC22E12.04 |ccs1|pccs, pccs|metallochaperone Ccs1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 297
Score = 27.1 bits (57), Expect = 3.7
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -3
Query: 281 SRTASGCRTTERDRGPTAACGLEIL*HSSCCRSNKVLCC 165
S+ GC +TE+ T+ C E SCC S K CC
Sbjct: 257 SQEKKGCCSTEK----TSCCSQE---KKSCCTSEKPSCC 288
>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
Length = 582
Score = 25.8 bits (54), Expect = 8.5
Identities = 13/43 (30%), Positives = 23/43 (53%)
Frame = +2
Query: 383 AKYPGLTVLLSVGGDADTEEPXKYXLLLESQQARTAFINSGVL 511
AK G ++ VGG ++ E K ++++ A A ++ GVL
Sbjct: 402 AKLSGGIAVIKVGGSSEVEVNEKKDRIVDALNAVKAAVSEGVL 444
>SPAC25B8.12c |||nucleotide-sugar phosphatase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 303
Score = 25.8 bits (54), Expect = 8.5
Identities = 15/51 (29%), Positives = 26/51 (50%)
Frame = +2
Query: 359 YRAITSLKAKYPGLTVLLSVGGDADTEEPXKYXLLLESQQARTAFINSGVL 511
YRA+ ++ KYP ++L+ G + + L L++ A A +N VL
Sbjct: 39 YRAMKYIREKYPNFPIVLATGKQRSAVDLIRIPLDLDAFPA--AHVNGCVL 87
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,634,006
Number of Sequences: 5004
Number of extensions: 44036
Number of successful extensions: 165
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 462505890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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