Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP07_F_P12
(894 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 146 3e-36
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 143 3e-35
SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFII... 29 0.89
SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc... 28 1.6
SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|c... 26 6.3
SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|... 26 8.3
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 146 bits (355), Expect = 3e-36
Identities = 78/128 (60%), Positives = 93/128 (72%)
Frame = +1
Query: 154 SEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRA 333
+ RED VY AKLAEQAERY+ MVE MK+VAS +D ELTVEERNLLSVAYKNVIGARRA
Sbjct: 4 TSREDAVYLAKLAEQAERYEGMVENMKSVAS---TDQELTVEERNLLSVAYKNVIGARRA 60
Query: 334 SWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSDILGVLXQVPHPQLTDRRXK 513
SWRI+SSIEQKEE+KG ++ +I+ YR ++E+EL IC DIL VL + P K
Sbjct: 61 SWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESK 120
Query: 514 SFLLXNEG 537
F +G
Sbjct: 121 VFYYKMKG 128
Score = 64.9 bits (151), Expect = 1e-11
Identities = 29/49 (59%), Positives = 36/49 (73%)
Frame = +2
Query: 476 KYLIPSSQTGXSKVFYYXMKGDYHRYLAXFATGNXRKEAAXXSLVAYKA 622
K+LIP++ + SKVFYY MKGDY+RYLA FA G R+ +A SL YKA
Sbjct: 108 KHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKA 156
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 143 bits (346), Expect = 3e-35
Identities = 75/128 (58%), Positives = 93/128 (72%)
Frame = +1
Query: 154 SEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRA 333
+ RE++VY AKLAEQAERY+EMVE MK VA N ++L+VEERNLLSVAYKN+IGARRA
Sbjct: 3 NSRENSVYLAKLAEQAERYEEMVENMKKVACSN---DKLSVEERNLLSVAYKNIIGARRA 59
Query: 334 SWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSDILGVLXQVPHPQLTDRRXK 513
SWRIISSIEQKEE++G + +I+ YR ++E EL DIC D+L VL + P T K
Sbjct: 60 SWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESK 119
Query: 514 SFLLXNEG 537
F +G
Sbjct: 120 VFYYKMKG 127
Score = 70.5 bits (165), Expect = 3e-13
Identities = 33/49 (67%), Positives = 37/49 (75%)
Frame = +2
Query: 476 KYLIPSSQTGXSKVFYYXMKGDYHRYLAXFATGNXRKEAAXXSLVAYKA 622
K+LIP++ TG SKVFYY MKGDY+RYLA F G KEAA SL AYKA
Sbjct: 107 KHLIPAATTGESKVFYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKA 155
>SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFIID
complex subunit Taf111|Schizosaccharomyces pombe|chr
1|||Manual
Length = 979
Score = 29.1 bits (62), Expect = 0.89
Identities = 21/85 (24%), Positives = 38/85 (44%)
Frame = +1
Query: 169 NVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRASWRII 348
N + +A+Q Y+E + + N R +S N NL +A K I + +
Sbjct: 779 NAHAYNVAKQQRAYEEEINRIWNAQKRGLSIN-------NLEELAKKYGINSIHDDYVES 831
Query: 349 SSIEQKEETKGAEDKLNMIRAYRSQ 423
+ +EET ++ L ++R YR +
Sbjct: 832 NEETTREETPSSDKVLRIVRLYRDK 856
>SPBC21.01 |mis17|SPBC776.19|kinetochore protein
Mis17|Schizosaccharomyces pombe|chr 2|||Manual
Length = 441
Score = 28.3 bits (60), Expect = 1.6
Identities = 23/100 (23%), Positives = 46/100 (46%)
Frame = +1
Query: 142 SSTMSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIG 321
SST S N ++ Q+E Y ++ + + + ++SD E +E R V +N +
Sbjct: 45 SSTRSSPASNQQNLEIVSQSEAYHKISDLDDSTSIFSISDQENILERRISEEVVRENSV- 103
Query: 322 ARRASWRIISSIEQKEETKGAEDKLNMIRAYRSQVEKELR 441
+S +SS ET+ + + L + S +K+++
Sbjct: 104 ---SSIENVSSSAVARETQSSANILVKDNHFFSHKQKKIQ 140
>SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 599
Score = 26.2 bits (55), Expect = 6.3
Identities = 10/37 (27%), Positives = 22/37 (59%)
Frame = -2
Query: 464 PRMSEQMSRSSFSTWLRYALIMFNLSSAPFVSSFCSM 354
P ++E + +S S+W+ A ++ + + P V FC++
Sbjct: 121 PTIAEDFNDTSSSSWIGSAYVLASNAVLPAVGVFCNI 157
>SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 736
Score = 25.8 bits (54), Expect = 8.3
Identities = 15/43 (34%), Positives = 20/43 (46%), Gaps = 2/43 (4%)
Frame = -3
Query: 271 SAHCRTRCVMLHSSSPPPFRHSAQPAQLTLLYT--HYLPFPTL 149
S+H + + H P F S QLTLL + YLP P +
Sbjct: 486 SSHREKKSIYQHKEYMPDFLSSRDEFQLTLLESTWQYLPDPNV 528
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,707,569
Number of Sequences: 5004
Number of extensions: 47182
Number of successful extensions: 150
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 450492750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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