Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP07_F_P10
(902 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 235 5e-63
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 226 4e-60
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 118 2e-27
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 101 1e-22
SPBC19G7.18c ||SPBC19G7.11c|sequence orphan|Schizosaccharomyces ... 26 6.4
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.4
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.4
SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ... 26 8.4
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 235 bits (576), Expect = 5e-63
Identities = 111/177 (62%), Positives = 133/177 (75%), Gaps = 1/177 (0%)
Frame = +3
Query: 147 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 326
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 327 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKXIVDLVLD 506
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GK +VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 507 RIRXLADQCTGLQGFLIFPLLRWRYRL-WGSLPY*WSVSPLTTGKKSKLEFAIYPXP 674
+IR +AD C+GLQGFL+F +G+L T KKSKL+F++YP P
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYT--KKSKLQFSVYPAP 175
Score = 28.7 bits (61), Expect = 1.2
Identities = 25/97 (25%), Positives = 38/97 (39%)
Frame = +2
Query: 590 GFTSLLMERLSVDYRQEV*TGVRHLPXALRFXXXXXXXXXXXXXXXXXXEHSDCAFHGST 769
GF +LL+ERL+++Y ++ P A + + +DC F
Sbjct: 148 GFGALLLERLAMEYTKKSKLQFSVYP-APQVSTSVVEPYNSVLTTHATLDLADCTFMVDN 206
Query: 770 MKPSMTFARXNXXIERPTYPNLESFHXDXLSPRTLXL 880
+ R N IERP+Y NL +S T L
Sbjct: 207 -ESCYDICRRNLDIERPSYENLNRLIAQVVSSITASL 242
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 226 bits (552), Expect = 4e-60
Identities = 110/181 (60%), Positives = 132/181 (72%), Gaps = 5/181 (2%)
Frame = +3
Query: 147 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 311
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 312 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKXIV 491
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GK ++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 492 DLVLDRIRXLADQCTGLQGFLIFPLLRWRYRLWGSLPY*WSVSPLTTGKKSKLEFAIYPX 671
D VL+RIR +AD C+GLQGFL+F G + GKKS L+F++YP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGS-GLGALLLERLNMEYGKKSNLQFSVYPA 178
Query: 672 P 674
P
Sbjct: 179 P 179
Score = 30.3 bits (65), Expect = 0.39
Identities = 26/97 (26%), Positives = 38/97 (39%)
Frame = +2
Query: 590 GFTSLLMERLSVDYRQEV*TGVRHLPXALRFXXXXXXXXXXXXXXXXXXEHSDCAFHGST 769
G +LL+ERL+++Y ++ P A + ++SDC F
Sbjct: 152 GLGALLLERLNMEYGKKSNLQFSVYP-APQVSTSVVEPYNSVLTTHATLDNSDCTFMVDN 210
Query: 770 MKPSMTFARXNXXIERPTYPNLESFHXDXLSPRTLXL 880
+ R N IERPTY NL +S T L
Sbjct: 211 -EACYDICRRNLDIERPTYENLNRLIAQVVSSITASL 246
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 118 bits (283), Expect = 2e-27
Identities = 57/135 (42%), Positives = 77/135 (57%)
Frame = +3
Query: 147 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 326
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 327 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKXIVDLVLD 506
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G + D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 507 RIRXLADQCTGLQGF 551
+R A+ C LQGF
Sbjct: 119 VVRREAEACDALQGF 133
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 101 bits (243), Expect = 1e-22
Identities = 53/136 (38%), Positives = 81/136 (59%), Gaps = 2/136 (1%)
Frame = +3
Query: 150 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 329
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 330 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKXIVDLVL 503
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 504 DRIRXLADQCTGLQGF 551
D I AD L+GF
Sbjct: 120 DMIDREADGSDSLEGF 135
>SPBC19G7.18c ||SPBC19G7.11c|sequence orphan|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 252
Score = 26.2 bits (55), Expect = 6.4
Identities = 18/52 (34%), Positives = 28/52 (53%), Gaps = 4/52 (7%)
Frame = +3
Query: 558 FPLLRWRYRLWGSLPY*WSVS---PLTTGKKSKLEFAIYPXPSGFHCR-RPA 701
FP +Y+L GS+P + S P T + ++ E A+Y +GF R +PA
Sbjct: 123 FPPKNSKYQLPGSMPNTGASSSQDPFTNSQSTEKEDAMYSKDNGFEDRSKPA 174
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.4
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +1
Query: 226 STASSLMARCPQTRPSGVETILSTLSSARPELAST 330
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.4
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 245 WPDAHRQDHRGWRRFFQHFLQR 310
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 509
Score = 25.8 bits (54), Expect = 8.4
Identities = 11/38 (28%), Positives = 18/38 (47%)
Frame = -2
Query: 586 SRYLHRRSGKIRNPCRPVHWSASXRILSKTKSTXSFPM 473
++Y+H + K R+ V W+ R L ST F +
Sbjct: 70 TKYIHTSANKARHVINVVRWTPDGRRLLTGSSTGEFTL 107
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,579,887
Number of Sequences: 5004
Number of extensions: 72867
Number of successful extensions: 186
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 183
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 456499320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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