Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP07_F_N18
(916 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.057
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 33 0.075
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 31 0.17
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.30
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 29 1.2
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 28 1.6
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 28 2.1
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 2.8
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 27 2.8
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 4.9
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 4.9
SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|... 26 6.5
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 26 6.5
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 26 6.5
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 26 6.5
SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 6.5
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 8.6
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 8.6
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 26 8.6
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 33.1 bits (72), Expect = 0.057
Identities = 17/52 (32%), Positives = 20/52 (38%), Gaps = 1/52 (1%)
Frame = +2
Query: 737 PPXPXPPXPPXLPXXXP-RVPPGAXTSPXXXXGSTXPPPTXTHXPLXXPXXP 889
PP P PP LP P +P GA +P + PP P P P
Sbjct: 420 PPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPP 471
Score = 32.7 bits (71), Expect = 0.075
Identities = 25/79 (31%), Positives = 29/79 (36%)
Frame = +3
Query: 654 PAHPPXX*SXXI*TSPPFNXLFSXPXYPPXXPPPPXPXXSPXXXXXSPRAPXXPPSXXXX 833
PA PP + T P + + P PP PP P P +P AP PPS
Sbjct: 401 PALPPLGNASRTSTPP----VPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIA 456
Query: 834 QLALPXXXHTXPWXPLXAP 890
LP P P AP
Sbjct: 457 P-PLPAGMPAAPPLPPAAP 474
Score = 31.5 bits (68), Expect = 0.17
Identities = 17/51 (33%), Positives = 19/51 (37%), Gaps = 3/51 (5%)
Frame = +2
Query: 737 PPXPXPPXPPXLPXXXPR---VPPGAXTSPXXXXGSTXPPPTXTHXPLXXP 880
PP P PP LP P +PP A +P G PP P P
Sbjct: 428 PPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPP 478
Score = 27.1 bits (57), Expect = 3.7
Identities = 19/62 (30%), Positives = 23/62 (37%), Gaps = 6/62 (9%)
Frame = +2
Query: 734 PPPXPXPPXPPXLPXXXP--RVP----PGAXTSPXXXXGSTXPPPTXTHXPLXXPXXPHP 895
P PP PP +P P RVP P P G+ PP P+ P +P
Sbjct: 224 PTSTSAPPIPPSIPSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPI-APVSMNP 282
Query: 896 XI 901
I
Sbjct: 283 AI 284
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 32.7 bits (71), Expect = 0.075
Identities = 21/60 (35%), Positives = 26/60 (43%), Gaps = 3/60 (5%)
Frame = +2
Query: 725 SXLPPPXPXPPXPPXLPXXXPRVPPGAXTSPXXXXGSTXPP--PTXTHXPLXXPXXP-HP 895
S LPP P P P +P + PP + +P ST PP + H P P P HP
Sbjct: 1483 SQLPPAVPNVPVPSMIPSVAQQ-PPSS-VAPATAPSSTLPPSQSSFAHVPSPAPPAPQHP 1540
Score = 27.1 bits (57), Expect = 3.7
Identities = 16/57 (28%), Positives = 20/57 (35%), Gaps = 3/57 (5%)
Frame = +2
Query: 734 PPPXPXPPXPPXLPXXXPRVPPGAXTSPXXXXGSTXPPPTXTHXPL---XXPXXPHP 895
PP P PP + P PP + P PP+ PL P P+P
Sbjct: 1690 PPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 31.5 bits (68), Expect = 0.17
Identities = 21/54 (38%), Positives = 23/54 (42%)
Frame = -2
Query: 894 GWGXKGXXKGXCVXVGGGXVXPXXMXGXVXAPGGTRGXXXGRXGGXGGXGXGGG 733
G+G +G G GGG P G PGG G G GG GG G G G
Sbjct: 212 GFGGEGHHHGGHGGFGGG---PGGFEG---GPGGFGGGPGGFGGGLGGFGGGPG 259
Score = 28.3 bits (60), Expect = 1.6
Identities = 20/57 (35%), Positives = 21/57 (36%), Gaps = 1/57 (1%)
Frame = -3
Query: 776 GGGXGG-GGGXGXGGVXRXGE*XIKGWXGSNXXASPAWGVGGXPGXLLITSWGFXXG 609
GGG GG GGG G G G+ G G GG PG GF G
Sbjct: 187 GGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGG 243
Score = 27.1 bits (57), Expect = 3.7
Identities = 17/50 (34%), Positives = 18/50 (36%)
Frame = -3
Query: 815 GXSGRPGGXXXXXGGGXGGGGGXGXGGVXRXGE*XIKGWXGSNXXASPAW 666
G G PGG GG GGG G GG+ G G P W
Sbjct: 225 GFGGGPGGFEGGPGG-FGGGPGGFGGGLGGFGGGPGGFGGGPGGHGGPGW 273
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 30.7 bits (66), Expect = 0.30
Identities = 18/60 (30%), Positives = 23/60 (38%), Gaps = 3/60 (5%)
Frame = +2
Query: 731 LPPPXPXPPXPPXLPXXXPRVPPGAXTSPXXXXGSTXPP---PTXTHXPLXXPXXPHPXI 901
+PPP PP P P VPP + P + PP PT P+ P P +
Sbjct: 1191 VPPPSEAPPVPKP-SVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVPAPSSEAPSV 1249
Score = 29.9 bits (64), Expect = 0.53
Identities = 18/61 (29%), Positives = 22/61 (36%)
Frame = +2
Query: 731 LPPPXPXPPXPPXLPXXXPRVPPGAXTSPXXXXGSTXPPPTXTHXPLXXPXXPHPXIXXX 910
+P P PP P P P VP + +P S PP P P P P +
Sbjct: 1076 IPAPSGAPPVPA--PSGIPPVPKPSVAAPPVPKPSVAVPPVPA--PSGAPPVPKPSVAAP 1131
Query: 911 P 913
P
Sbjct: 1132 P 1132
Score = 28.7 bits (61), Expect = 1.2
Identities = 15/53 (28%), Positives = 19/53 (35%)
Frame = +3
Query: 735 PPXXPPPPXPXXSPXXXXXSPRAPXXPPSXXXXQLALPXXXHTXPWXPLXAPT 893
P PP P P +P S AP PP +P P+ AP+
Sbjct: 1037 PSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPS 1089
Score = 25.8 bits (54), Expect = 8.6
Identities = 19/65 (29%), Positives = 22/65 (33%), Gaps = 6/65 (9%)
Frame = +2
Query: 725 SXLPPPXPXP----PXPPXLPXXXPRVPPGAXTSPXXXXGSTXP--PPTXTHXPLXXPXX 886
S PP P P P P P VPP + P P PP T P+ P
Sbjct: 1165 SVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSA 1224
Query: 887 PHPXI 901
P +
Sbjct: 1225 GLPPV 1229
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 28.7 bits (61), Expect = 1.2
Identities = 16/43 (37%), Positives = 19/43 (44%)
Frame = -3
Query: 815 GXSGRPGGXXXXXGGGXGGGGGXGXGGVXRXGE*XIKGWXGSN 687
G +G GG GG GGG G GG R G +G G +
Sbjct: 20 GFNGGRGGFGGGRGGARGGGRGGARGG--RGGRGGARGGRGGS 60
Score = 27.5 bits (58), Expect = 2.8
Identities = 21/57 (36%), Positives = 22/57 (38%)
Frame = -3
Query: 815 GXSGRPGGXXXXXGGGXGGGGGXGXGGVXRXGE*XIKGWXGSNXXASPAWGVGGXPG 645
G G G GGG GG G G GG R G G G +S G GG G
Sbjct: 16 GGRGGFNGGRGGFGGGRGGARGGGRGGA-RGGRGGRGGARGGRGGSS--GGRGGAKG 69
Score = 25.8 bits (54), Expect = 8.6
Identities = 15/40 (37%), Positives = 17/40 (42%)
Frame = -1
Query: 817 GGXXGARGDXXXXXGEXXGXGGGGXWGG*XGXENSXLKGG 698
GG G+RG G G GGG GG G +GG
Sbjct: 9 GGRGGSRGGRGGFNGGRGGFGGGR--GGARGGGRGGARGG 46
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 28.3 bits (60), Expect = 1.6
Identities = 29/131 (22%), Positives = 52/131 (39%)
Frame = +2
Query: 83 PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 262
P S S + A + L + V R F + +EH+ K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229
Query: 263 SKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGXAKEALEQSRQNIXRTAEELRK 442
SKDA A S+ + S + +L N ++ +L+ ++ R
Sbjct: 230 SKDASSNLTACNRSGSSLSSNFYSSRLSKKTSLASLN-KSRASLQHKIMSLSRNIIRRVF 288
Query: 443 XHPDVEKNATA 475
P+V + +A
Sbjct: 289 HKPEVHLDPSA 299
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 27.9 bits (59), Expect = 2.1
Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
Frame = +2
Query: 170 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT---KSKDAQDFSKAWKDGSESVLQQLNAFA 340
++ DFFK + ++ H TL ++ NSL+ +K + G + +LN
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLSSFLSTKSTSASENKFHGGLNWLSLKLNLLL 115
Query: 341 KSLQGALGDA 370
+ LQG + A
Sbjct: 116 R-LQGRMNSA 124
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 27.5 bits (58), Expect = 2.8
Identities = 18/63 (28%), Positives = 22/63 (34%), Gaps = 2/63 (3%)
Frame = +2
Query: 731 LPPPXPX--PPXPPXLPXXXPRVPPGAXTSPXXXXGSTXPPPTXTHXPLXXPXXPHPXIX 904
+PPP P PP P P +PP P PP+ P P P P +
Sbjct: 149 IPPPSPASAPPIPSKAPPIPSSLPP-----PAQPAAPVKSPPSAPSLPSAVPPMP-PKVP 202
Query: 905 XXP 913
P
Sbjct: 203 PPP 205
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 27.5 bits (58), Expect = 2.8
Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 2/54 (3%)
Frame = +2
Query: 275 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGXAKEALEQSR-QNIXRTAE 430
+D + A+ + SVLQ+L+ + +QG LG N AL Q + QN+ E
Sbjct: 79 EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 4.9
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +2
Query: 176 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 295
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 26.6 bits (56), Expect = 4.9
Identities = 14/45 (31%), Positives = 17/45 (37%)
Frame = +2
Query: 716 ILXSXLPPPXPXPPXPPXLPXXXPRVPPGAXTSPXXXXGSTXPPP 850
I+ + P P P PP P + P PP P PPP
Sbjct: 740 IVPTPAPAPIPVPPPAPIMGGPPPPPPP-----PGVAGAGPPPPP 779
Score = 26.2 bits (55), Expect = 6.5
Identities = 14/48 (29%), Positives = 15/48 (31%)
Frame = +2
Query: 752 PPXPPXLPXXXPRVPPGAXTSPXXXXGSTXPPPTXTHXPLXXPXXPHP 895
PP PP + P P P G PPP P P P
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
>SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1372
Score = 26.2 bits (55), Expect = 6.5
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = +2
Query: 734 PPPXPXPPXPPXLP 775
PPP P PP P LP
Sbjct: 806 PPPAPLPPPAPPLP 819
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 26.2 bits (55), Expect = 6.5
Identities = 13/25 (52%), Positives = 13/25 (52%), Gaps = 2/25 (8%)
Frame = +2
Query: 734 PPPXPXPP--XPPXLPXXXPRVPPG 802
PPP P PP PP P P PPG
Sbjct: 9 PPPPPPPPGFEPPSQPPPPP--PPG 31
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 26.2 bits (55), Expect = 6.5
Identities = 14/28 (50%), Positives = 14/28 (50%), Gaps = 1/28 (3%)
Frame = -3
Query: 815 GXSGRPGGXXXXXG-GGXGGGGGXGXGG 735
G G GG G GG GGG G G GG
Sbjct: 451 GGRGGFGGRGGFGGRGGFGGGRGRGRGG 478
>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 321
Score = 26.2 bits (55), Expect = 6.5
Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
Frame = +2
Query: 212 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQ-GALGDA 370
+K+ + E+Q +T DFS K + L+AF K L+ + GDA
Sbjct: 89 SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASSTGDA 142
>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 168
Score = 26.2 bits (55), Expect = 6.5
Identities = 12/46 (26%), Positives = 23/46 (50%)
Frame = +2
Query: 296 KDGSESVLQQLNAFAKSLQGALGDANGXAKEALEQSRQNIXRTAEE 433
KDG+++ +LN FAK L + + A + + I +++ E
Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 25.8 bits (54), Expect = 8.6
Identities = 16/53 (30%), Positives = 20/53 (37%), Gaps = 4/53 (7%)
Frame = +2
Query: 734 PPPXPXPPXPPXLP---XXXPRVP-PGAXTSPXXXXGSTXPPPTXTHXPLXXP 880
P P P PP PP LP ++P P + + PPP P P
Sbjct: 234 PLPAPPPPPPPTLPPQSTNTSQLPMPSRNVNNLGSQVNIPPPPATPSQPPRPP 286
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 8.6
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +2
Query: 140 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 316
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 317 LQQL 328
Q+
Sbjct: 73 NNQI 76
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 25.8 bits (54), Expect = 8.6
Identities = 15/41 (36%), Positives = 16/41 (39%), Gaps = 1/41 (2%)
Frame = -1
Query: 817 GGXXGARG-DXXXXXGEXXGXGGGGXWGG*XGXENSXLKGG 698
GG G RG G G GGG GG G +GG
Sbjct: 141 GGFRGGRGGSRGGFGGNSRGGFGGGSRGGFGGGSRGGSRGG 181
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,557,663
Number of Sequences: 5004
Number of extensions: 43610
Number of successful extensions: 319
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 166
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 244
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 464508080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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