Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP07_F_M21
(1085 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 31 0.21
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 30 0.49
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 0.86
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 29 1.1
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 29 1.1
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 1.1
SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyc... 28 2.0
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 2.1
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 2.6
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 4.6
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 27 6.0
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 27 6.0
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 31.5 bits (68), Expect = 0.21
Identities = 18/46 (39%), Positives = 20/46 (43%)
Frame = -3
Query: 471 GGVXGRRXPXGGXXKXGERGGGAGXXGXGGGGQXXFXXXPGGXGXG 334
GG G R GG + G RGGG G G GG+ GG G
Sbjct: 19 GGFNGGRGGFGGG-RGGARGGGRGGARGGRGGRGGARGGRGGSSGG 63
Score = 30.3 bits (65), Expect = 0.49
Identities = 15/38 (39%), Positives = 17/38 (44%)
Frame = -1
Query: 458 GGAXPXGGXXRXGKGGAGRXXXGXGGGGRXGFXXXPGG 345
GG+ G G+GG G G GGGR G GG
Sbjct: 12 GGSRGGRGGFNGGRGGFGGGRGGARGGGRGGARGGRGG 49
Score = 26.6 bits (56), Expect = 6.0
Identities = 15/34 (44%), Positives = 16/34 (47%), Gaps = 2/34 (5%)
Frame = -1
Query: 440 GGXXRXGKGGA--GRXXXGXGGGGRXGFXXXPGG 345
GG G+GGA GR G GGR G GG
Sbjct: 33 GGARGGGRGGARGGRGGRGGARGGRGGSSGGRGG 66
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 30.3 bits (65), Expect = 0.49
Identities = 19/46 (41%), Positives = 19/46 (41%)
Frame = -3
Query: 471 GGVXGRRXPXGGXXKXGERGGGAGXXGXGGGGQXXFXXXPGGXGXG 334
GG G GG G GG G G GGG F PGG G G
Sbjct: 221 GGHGGFGGGPGGFE--GGPGGFGGGPGGFGGGLGGFGGGPGGFGGG 264
Score = 29.9 bits (64), Expect = 0.65
Identities = 17/47 (36%), Positives = 18/47 (38%)
Frame = -3
Query: 471 GGVXGRRXPXGGXXKXGERGGGAGXXGXGGGGQXXFXXXPGGXGXGV 331
GG G G G GG G G GG F PGG G G+
Sbjct: 205 GGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGL 251
Score = 29.9 bits (64), Expect = 0.65
Identities = 18/44 (40%), Positives = 18/44 (40%)
Frame = -3
Query: 471 GGVXGRRXPXGGXXKXGERGGGAGXXGXGGGGQXXFXXXPGGXG 340
GG G GG G GG G G GGG F PGG G
Sbjct: 228 GGPGGFEGGPGGFG--GGPGGFGGGLGGFGGGPGGFGGGPGGHG 269
Score = 28.7 bits (61), Expect = 1.5
Identities = 16/36 (44%), Positives = 16/36 (44%), Gaps = 2/36 (5%)
Frame = -1
Query: 446 PXGGXXRXGKGGAGRXXXGXGG--GGRXGFXXXPGG 345
P G G GG G G GG GG GF PGG
Sbjct: 204 PGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGG 239
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 29.5 bits (63), Expect = 0.86
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = +2
Query: 857 PAPPPPPPXXXPSXXPP 907
P PPPPPP P PP
Sbjct: 9 PPPPPPPPGFEPPSQPP 25
Score = 27.9 bits (59), Expect = 2.6
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = +2
Query: 857 PAPPPPPPXXXPSXXPP 907
P PPPPP PS PP
Sbjct: 10 PPPPPPPGFEPPSQPPP 26
Score = 27.5 bits (58), Expect = 3.5
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +1
Query: 1018 PPXXXXXAXPPXVPPXPPPP 1077
PP PP PP PPPP
Sbjct: 11 PPPPPPGFEPPSQPPPPPPP 30
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 29.1 bits (62), Expect = 1.1
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = +2
Query: 857 PAPPPPPPXXXPSXXPP 907
PAPPPPPP P+ P
Sbjct: 3 PAPPPPPPAPAPAAAAP 19
Score = 27.9 bits (59), Expect = 2.6
Identities = 14/41 (34%), Positives = 16/41 (39%), Gaps = 3/41 (7%)
Frame = +1
Query: 346 PPGXXXKPXLPPPPX---PXXXRPAPPFPXLXXPPXGXAPP 459
P +P +PPP P APP P PP A P
Sbjct: 140 PTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAP 180
Score = 27.5 bits (58), Expect = 3.5
Identities = 13/38 (34%), Positives = 14/38 (36%), Gaps = 1/38 (2%)
Frame = +1
Query: 346 PPGXXXKPXLPPPPXPXXXRPAPPFPX-LXXPPXGXAP 456
PP P PP P PP P + PP AP
Sbjct: 173 PPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPPLSQAP 210
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 29.1 bits (62), Expect = 1.1
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = +2
Query: 860 APPPPPPXXXPSXXPP 907
APPPPPP P PP
Sbjct: 1881 APPPPPPMALPKAGPP 1896
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 29.1 bits (62), Expect = 1.1
Identities = 13/25 (52%), Positives = 13/25 (52%)
Frame = +1
Query: 1009 PXTPPXXXXXAXPPXVPPXPPPPXV 1083
P PP A PP PP PPPP V
Sbjct: 763 PPPPPPGVAGAGPP--PPPPPPPAV 785
Score = 26.6 bits (56), Expect = 6.0
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = +2
Query: 857 PAPPPPPPXXXPSXXPP 907
P PPPPPP + PP
Sbjct: 761 PPPPPPPPGVAGAGPPP 777
Score = 26.2 bits (55), Expect = 8.0
Identities = 13/37 (35%), Positives = 13/37 (35%)
Frame = +1
Query: 349 PGXXXKPXLPPPPXPXXXRPAPPFPXLXXPPXGXAPP 459
P P PPP P P PP P G PP
Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPP 778
>SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 379
Score = 28.3 bits (60), Expect = 2.0
Identities = 16/46 (34%), Positives = 18/46 (39%)
Frame = -3
Query: 471 GGVXGRRXPXGGXXKXGERGGGAGXXGXGGGGQXXFXXXPGGXGXG 334
GG+ G GG + GG G GGG F GG G G
Sbjct: 137 GGMGGGMGGMGGMDDDMDMDGGFGTRTRGGGMPGGFANMFGGGGAG 182
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.9 bits (59), Expect = 2.6
Identities = 17/43 (39%), Positives = 17/43 (39%), Gaps = 6/43 (13%)
Frame = +1
Query: 346 PPGXXXKPXLPP-----PPXPXXXRPAPPF-PXLXXPPXGXAP 456
P G P LPP PP P APP P PP AP
Sbjct: 440 PMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAP 482
Score = 25.8 bits (54), Expect(2) = 2.1
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = +2
Query: 860 APPPPPPXXXPS 895
APPPPPP PS
Sbjct: 360 APPPPPPRSAPS 371
Score = 23.4 bits (48), Expect(2) = 4.6
Identities = 8/15 (53%), Positives = 8/15 (53%)
Frame = +2
Query: 863 PPPPPPXXXPSXXPP 907
PPPPPP PP
Sbjct: 312 PPPPPPSRRNRGKPP 326
Score = 21.8 bits (44), Expect(2) = 4.6
Identities = 7/12 (58%), Positives = 8/12 (66%)
Frame = +2
Query: 842 AXRXXPAPPPPP 877
A + P PPPPP
Sbjct: 306 ANKKRPPPPPPP 317
Score = 20.6 bits (41), Expect(2) = 2.1
Identities = 6/7 (85%), Positives = 6/7 (85%)
Frame = +2
Query: 857 PAPPPPP 877
P PPPPP
Sbjct: 337 PPPPPPP 343
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 2.6
Identities = 11/26 (42%), Positives = 12/26 (46%)
Frame = +1
Query: 346 PPGXXXKPXLPPPPXPXXXRPAPPFP 423
PP P PPPP P P+ P P
Sbjct: 1721 PPMPAGPPSAPPPPLPASSAPSVPNP 1746
Score = 27.1 bits (57), Expect = 4.6
Identities = 17/41 (41%), Positives = 17/41 (41%), Gaps = 1/41 (2%)
Frame = +1
Query: 340 AXPPGXXXKPXLPPPPXPXXXRP-APPFPXLXXPPXGXAPP 459
A PP P PPPP P APP P PP PP
Sbjct: 1697 AAPP-QMSAPTPPPPPMSVPPPPSAPPMP--AGPPSAPPPP 1734
Score = 26.6 bits (56), Expect = 6.0
Identities = 15/43 (34%), Positives = 18/43 (41%)
Frame = +1
Query: 331 HXXAXPPGXXXKPXLPPPPXPXXXRPAPPFPXLXXPPXGXAPP 459
H + PP +P PP P PP P + PP APP
Sbjct: 1685 HPVSTPP---VRPQSAAPPQ--MSAPTPPPPPMSVPPPPSAPP 1722
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 27.1 bits (57), Expect = 4.6
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = +2
Query: 857 PAPPPPPPXXXP 892
PAPPPPPP P
Sbjct: 236 PAPPPPPPPTLP 247
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 26.6 bits (56), Expect = 6.0
Identities = 16/32 (50%), Positives = 17/32 (53%), Gaps = 1/32 (3%)
Frame = -1
Query: 440 GGXXRXGKGGAGRXXXGXGG-GGRXGFXXXPG 348
GG R G+GG G G GG GGR GF G
Sbjct: 446 GGGSRGGRGGFG----GRGGFGGRGGFGGGRG 473
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 26.6 bits (56), Expect = 6.0
Identities = 15/43 (34%), Positives = 17/43 (39%), Gaps = 1/43 (2%)
Frame = -3
Query: 459 GRRXPXGGXXKXGERGGGAGXXGXGGGGQXXFXXXP-GGXGXG 334
G + P G RGG G G GG + F GG G G
Sbjct: 123 GPKKPKGARNGPAGRGGRGGFRGGRGGSRGGFGGNSRGGFGGG 165
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,287,426
Number of Sequences: 5004
Number of extensions: 15270
Number of successful extensions: 313
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 51
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 238
length of database: 2,362,478
effective HSP length: 74
effective length of database: 1,992,182
effective search space used: 571756234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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