Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP07_F_I15
         (866 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   175   5e-45
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   168   1e-42
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    63   4e-11

>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  175 bits (427), Expect = 5e-45
 Identities = 85/126 (67%), Positives = 97/126 (76%)
 Frame = -1

Query: 593 LLYXVPVVTSXPRNVXAAIGYHXNQXYYPIRXLXPTGFKVGIXYQPPTVXPGGDLAKVQR 414
           LLY   V+   PR+V AA+    ++         PTGFK+GI Y+PP   PG  +AKV R
Sbjct: 321 LLYRGDVI---PRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNR 377

Query: 413 AVCMLSNTTAIAEAWARLDHKFDLMYAKXAFVHWYVGEGMEEGEFSEAREDLAALEKDYE 234
           AVCMLSNTT+IAEAW+RLDHKFDLMY+K AFVHWYVGEGMEEGEFSEAREDLAALE+DYE
Sbjct: 378 AVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYE 437

Query: 233 EVGMDS 216
           EVG DS
Sbjct: 438 EVGQDS 443



 Score = 33.9 bits (74), Expect = 0.030
 Identities = 17/38 (44%), Positives = 22/38 (57%)
 Frame = -3

Query: 666 ITNAWXEPANQMXKWDPRSXQXXGLAVXXTRGDVXPKE 553
           ITN   EP NQM K DPR+ +     +   RGDV P++
Sbjct: 295 ITNQCFEPYNQMVKCDPRTGRYMATCLLY-RGDVIPRD 331


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  168 bits (408), Expect = 1e-42
 Identities = 83/126 (65%), Positives = 95/126 (75%)
 Frame = -1

Query: 593 LLYXVPVVTSXPRNVXAAIGYHXNQXYYPIRXLXPTGFKVGIXYQPPTVXPGGDLAKVQR 414
           LLY   V+   PR+V AA+     +         PTGFK+GI  +PP    G ++AKV R
Sbjct: 317 LLYRGDVI---PRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDR 373

Query: 413 AVCMLSNTTAIAEAWARLDHKFDLMYAKXAFVHWYVGEGMEEGEFSEAREDLAALEKDYE 234
           AVCMLSNTT+IAEAW+RLDHKFDLMY+K AFVHWYVGEGMEEGEFSEAREDLAALE+DYE
Sbjct: 374 AVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYE 433

Query: 233 EVGMDS 216
           EVG DS
Sbjct: 434 EVGQDS 439



 Score = 33.9 bits (74), Expect = 0.030
 Identities = 17/38 (44%), Positives = 22/38 (57%)
 Frame = -3

Query: 666 ITNAWXEPANQMXKWDPRSXQXXGLAVXXTRGDVXPKE 553
           ITN   EP NQM K DPR+ +     +   RGDV P++
Sbjct: 291 ITNQCFEPYNQMVKCDPRAGRYMATCLLY-RGDVIPRD 327


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 63.3 bits (147), Expect = 4e-11
 Identities = 28/71 (39%), Positives = 45/71 (63%), Gaps = 3/71 (4%)
 Frame = -1

Query: 422 VQRAVCMLSNTTAIAEAWARLDHKFDLMYAKXAFVHWYVGEGMEEGEFSEAR---EDLAA 252
           ++ +   + N+T+I E + RL  +F  M+ + AF+HWY GEGM+E EF+EA     DL +
Sbjct: 361 LKMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS 420

Query: 251 LEKDYEEVGMD 219
             + Y+E G+D
Sbjct: 421 EYQQYQEAGID 431


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,519,074
Number of Sequences: 5004
Number of extensions: 38950
Number of successful extensions: 92
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 84
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 91
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 432473040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)