Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP07_F_G05
(870 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 212 5e-56
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 205 6e-54
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 106 4e-24
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 95 2e-20
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.0
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.0
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 212 bits (518), Expect = 5e-56
Identities = 92/132 (69%), Positives = 109/132 (82%)
Frame = +1
Query: 145 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 324
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 325 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVXLVLX 504
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+V V
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 505 RIXKLADXCTGL 540
+I ++AD C+GL
Sbjct: 121 KIRRIADNCSGL 132
Score = 55.6 bits (128), Expect = 9e-09
Identities = 25/56 (44%), Positives = 35/56 (62%)
Frame = +3
Query: 546 FLIFHXXXXXXXXXXXXLLMERLSVDYRQKSKLEFXIYPRLXFPLAVVXPYNSILT 713
FL+FH LL+ERL+++Y +KSKL+F +YP +VV PYNS+LT
Sbjct: 135 FLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLT 190
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 205 bits (501), Expect = 6e-54
Identities = 94/137 (68%), Positives = 111/137 (81%), Gaps = 5/137 (3%)
Frame = +1
Query: 145 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 309
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 310 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 489
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 490 XLVLXRIXKLADXCTGL 540
VL RI ++AD C+GL
Sbjct: 120 DSVLERIRRMADNCSGL 136
Score = 53.2 bits (122), Expect = 5e-08
Identities = 24/56 (42%), Positives = 34/56 (60%)
Frame = +3
Query: 546 FLIFHXXXXXXXXXXXXLLMERLSVDYRQKSKLEFXIYPRLXFPLAVVXPYNSILT 713
FL+FH LL+ERL+++Y +KS L+F +YP +VV PYNS+LT
Sbjct: 139 FLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLT 194
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 106 bits (255), Expect = 4e-24
Identities = 52/132 (39%), Positives = 73/132 (55%)
Frame = +1
Query: 145 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 324
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 325 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVXLVLX 504
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ VL
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 505 RIXKLADXCTGL 540
+ + A+ C L
Sbjct: 119 VVRREAEACDAL 130
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 94.7 bits (225), Expect = 2e-20
Identities = 50/133 (37%), Positives = 77/133 (57%), Gaps = 2/133 (1%)
Frame = +1
Query: 148 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 327
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 328 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVXLVL 501
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 502 XRIXKLADXCTGL 540
I + AD L
Sbjct: 120 DMIDREADGSDSL 132
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.0
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 224 STASSLMARCPQTRPSGVETILSTLSSARPELAST 328
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.0
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 243 WPDAHRQDHRGWRRFFQHFLQR 308
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,274,455
Number of Sequences: 5004
Number of extensions: 61360
Number of successful extensions: 141
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 138
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 434475230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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