Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP07_F_F17
(883 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 221 1e-58
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 111 2e-25
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 109 7e-25
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 103 3e-23
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 63 4e-11
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 61 2e-10
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 59 9e-10
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 37 0.003
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.50
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 29 0.88
SPCC16C4.08c |skb15||Shk1 kinase binding protein 15|Schizosaccha... 26 6.2
SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 26 8.2
SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pom... 26 8.2
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 221 bits (540), Expect = 1e-58
Identities = 113/160 (70%), Positives = 117/160 (73%)
Frame = +2
Query: 275 PAIRGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVA 454
P G+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVA
Sbjct: 38 PRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVA 97
Query: 455 PEEHPVLLTEAPLNPKANREXMXXIMFETFNTPAMYVAIQAVLSLYASXXXXXXXXXXXX 634
PEEHP LLTEAPLNPK+NRE M I+FETFN PA YVAIQAVLSLYAS
Sbjct: 98 PEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGD 157
Query: 635 KVXPTPWPIXEGYPLPPXHPGVWT*PGRXLXDYLMNIXTE 754
V T PI EGY LP + GR L DYLM I E
Sbjct: 158 GVTHTV-PIYEGYALPHAIMRL-DLAGRDLTDYLMKILME 195
Score = 86.6 bits (205), Expect = 4e-18
Identities = 37/41 (90%), Positives = 40/41 (97%)
Frame = +1
Query: 166 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG 288
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHG 42
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 111 bits (266), Expect = 2e-25
Identities = 48/95 (50%), Positives = 70/95 (73%)
Frame = +2
Query: 311 KDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 490
KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 491 LNPKANREXMXXIMFETFNTPAMYVAIQAVLSLYA 595
+NP ANRE M MFE + +YVAIQAVLSLYA
Sbjct: 110 MNPVANREKMCETMFERYGFGGVYVAIQAVLSLYA 144
Score = 48.0 bits (109), Expect = 2e-06
Identities = 21/35 (60%), Positives = 26/35 (74%)
Frame = +1
Query: 172 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 276
E A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 109 bits (261), Expect = 7e-25
Identities = 55/132 (41%), Positives = 79/132 (59%), Gaps = 1/132 (0%)
Frame = +2
Query: 290 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEH 466
VM QKD +VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 467 PVLLTEAPLNPKANREXMXXIMFETFNTPAMYVAIQAVLSLYASXXXXXXXXXXXXKVXP 646
P+LLTE PL N+E + +ET N PA+ ++Q VL+LYAS +
Sbjct: 107 PLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTH 166
Query: 647 TPWPIXEGYPLP 682
+ PI +G+ +P
Sbjct: 167 SV-PIYDGFSIP 177
Score = 52.4 bits (120), Expect = 8e-08
Identities = 20/34 (58%), Positives = 27/34 (79%)
Frame = +1
Query: 184 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 285
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHE 45
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 103 bits (248), Expect = 3e-23
Identities = 47/96 (48%), Positives = 62/96 (64%), Gaps = 1/96 (1%)
Frame = +2
Query: 314 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 490
D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE P
Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132
Query: 491 LNPKANREXMXXIMFETFNTPAMYVAIQAVLSLYAS 598
LNP NRE IMFE+FN +Y+A+QAVL+L AS
Sbjct: 133 LNPPENRENTAEIMFESFNCAGLYIAVQAVLALAAS 168
Score = 40.7 bits (91), Expect = 3e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +1
Query: 184 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 267
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 63.3 bits (147), Expect = 4e-11
Identities = 30/81 (37%), Positives = 43/81 (53%)
Frame = +2
Query: 356 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREXMXXIMF 535
+ +K I +G V NWD +W + +L+ P EHP+L+TE NP NR MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 536 ETFNTPAMYVAIQAVLSLYAS 598
E+ PA Y+A Q + +AS
Sbjct: 123 ESLRCPATYLAKQETCAAFAS 143
Score = 40.3 bits (90), Expect = 4e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +1
Query: 169 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 270
+EV+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 60.9 bits (141), Expect = 2e-10
Identities = 29/98 (29%), Positives = 54/98 (55%), Gaps = 1/98 (1%)
Frame = +2
Query: 302 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL 478
+ + + VG++ + G ++ + P E +++NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 479 TEAPLNPKANREXMXXIMFETFNTPAMYVAIQAVLSLY 592
TE NP R M ++FE +N P++ I + S Y
Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFY 158
Score = 35.9 bits (79), Expect = 0.008
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +1
Query: 184 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 279
LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 58.8 bits (136), Expect = 9e-10
Identities = 32/110 (29%), Positives = 56/110 (50%), Gaps = 2/110 (1%)
Frame = +2
Query: 275 PAIRGVMVGMGQKDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELR 448
P+ GV + ++ YV DE Q I + +K +GI+ +W+ W +L+
Sbjct: 37 PSYYGVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQ 96
Query: 449 VAPEEHPVLLTEAPLNPKANREXMXXIMFETFNTPAMYVAIQAVLSLYAS 598
V P E+ +++TE NP++ R+ + FE + PA Y+ QAV +A+
Sbjct: 97 VNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFAN 146
Score = 36.7 bits (81), Expect = 0.004
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +1
Query: 169 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 270
EE+ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 37.1 bits (82), Expect = 0.003
Identities = 22/79 (27%), Positives = 35/79 (44%), Gaps = 4/79 (5%)
Frame = +2
Query: 371 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREXMXXIMFE 538
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 539 TFNTPAMYVAIQAVLSLYA 595
P +A + ++ LYA
Sbjct: 172 ECQVPGFTIAYEPLMGLYA 190
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 29.9 bits (64), Expect = 0.50
Identities = 19/53 (35%), Positives = 29/53 (54%), Gaps = 5/53 (9%)
Frame = +2
Query: 431 FYNELRVAPEEHPVLLTE--APLNPKANREXMXXIMFETFNTPAM---YVAIQ 574
+Y L E+HP+LLT+ A L P+ + + I ++ NTP + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 29.1 bits (62), Expect = 0.88
Identities = 19/60 (31%), Positives = 25/60 (41%)
Frame = +3
Query: 432 STMSCVSPPRNTQSCSLRLPSTPRPTERX*XXSCSKHSTRPPCTSPSKPCSRCTRPVVPP 611
ST S S P T S S +PS ++ +H T P +K SR P+ PP
Sbjct: 127 STPSISSIPHTTSSVSNDIPSVLGSSDHPIDLDNPEHLTPPSSFITAKQLSRLPTPLPPP 186
>SPCC16C4.08c |skb15||Shk1 kinase binding protein
15|Schizosaccharomyces pombe|chr 3|||Manual
Length = 341
Score = 26.2 bits (55), Expect = 6.2
Identities = 14/38 (36%), Positives = 17/38 (44%)
Frame = +1
Query: 568 HPSRALAVRVRSYHRVSCWDXGEGVSHTVAHLXRIPTS 681
HPS LA+ V ++ WD G V L IP S
Sbjct: 129 HPSEKLALTVGGDGKLRLWDLVRGKGGKVLPLSTIPES 166
>SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase
Tor2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2337
Score = 25.8 bits (54), Expect = 8.2
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = -1
Query: 367 FEGQDTSFALCLISYIRVLLSHTDHHA 287
+EG+DT+ +Y+R++L TD A
Sbjct: 74 YEGEDTTRITRFANYLRIILPGTDQKA 100
>SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 204
Score = 25.8 bits (54), Expect = 8.2
Identities = 22/107 (20%), Positives = 37/107 (34%), Gaps = 1/107 (0%)
Frame = +2
Query: 374 IEHGIVTNWDDMEKIWHHTFYNELRVAPE-EHPVLLTEAPLNPKANREXMXXIMFETFNT 550
+ +G V WD+ K + + ++ + P+ E PV P P + +++
Sbjct: 9 LPNGWVAQWDERYKCYFYVNESDPKAKPQWECPVRGLTIPPPPSVDHSAPPSGPPPSYSN 68
Query: 551 PAMYVAIQAVLSLYASXXXXXXXXXXXXKVXPTPWPIXEGYPLPPXH 691
A A S A P P+P GYP P +
Sbjct: 69 SAAPATPAASASSAAPAPAPAASQNRAYGAAPQPYPPQGGYPQQPYY 115
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,260,120
Number of Sequences: 5004
Number of extensions: 65084
Number of successful extensions: 172
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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