Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP07_F_A18
(888 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 213 3e-56
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 205 7e-54
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 95 9e-21
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 90 3e-19
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 31 0.17
SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces po... 27 3.6
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.2
SPCC1682.15 |mug122||PX/PXA domain protein|Schizosaccharomyces p... 26 8.2
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.2
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 213 bits (520), Expect = 3e-56
Identities = 105/172 (61%), Positives = 124/172 (72%), Gaps = 1/172 (0%)
Frame = +3
Query: 144 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 323
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 324 HVPRAVFVDLEXTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGSLHHWKGNRRFG-F 500
+VPR+++VDLE V+D+VRTG YR LFHPEQLITGKEDA+NNYARG H+ G
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARG--HYTVGKELVDEV 118
Query: 501 GQKSASSLXSVPGLQGFLIFHSFGGRYRLWGSLPLLMERLSVDYGKKSKLEF 656
K + GLQGFL+FHSFGG G LL+ERL+++Y KKSKL+F
Sbjct: 119 TDKIRRIADNCSGLQGFLVFHSFGGGTGS-GFGALLLERLAMEYTKKSKLQF 169
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 205 bits (500), Expect = 7e-54
Identities = 105/177 (59%), Positives = 126/177 (71%), Gaps = 6/177 (3%)
Frame = +3
Query: 144 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 308
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 309 TGAGKHVPRAVFVDLEXTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGSLHHWKGNR 488
TG GK VPR+++VDLE V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARG H+ G
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARG--HYTVGKE 117
Query: 489 RF-GFGQKSASSLXSVPGLQGFLIFHSFGGRYRLWGSLPLLMERLSVDYGKKSKLEF 656
++ + GLQGFL+FHSFGG G LL+ERL+++YGKKS L+F
Sbjct: 118 MIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGS-GLGALLLERLNMEYGKKSNLQF 173
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 95.5 bits (227), Expect = 9e-21
Identities = 62/169 (36%), Positives = 88/169 (52%), Gaps = 4/169 (2%)
Frame = +3
Query: 147 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 326
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 327 VPRAVFVDLEXTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGSLHHWKGNRRF-G 497
+PRA+ +DLE VV+ + + TY L++PE ++ K A NN+A G H R F
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANGYSH---AERIFED 117
Query: 498 FGQKSASSLXSVPGLQGFLIFHSF-GGRYRLWGSLPLLMERLSVDYGKK 641
L+GF + HS GG GS L+ERL+ Y KK
Sbjct: 118 IMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGS--FLLERLNDRYPKK 164
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 90.2 bits (214), Expect = 3e-19
Identities = 56/172 (32%), Positives = 82/172 (47%), Gaps = 1/172 (0%)
Frame = +3
Query: 144 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 323
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 324 HVPRAVFVDLEXTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGSLHHWKG-NRRFGF 500
+VPRAV VDLE +D V++G + LF P+ +I G+ A N +A+G H+ +G
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKG--HYTEGAELADAV 116
Query: 501 GQKSASSLXSVPGLQGFLIFHSFGGRYRLWGSLPLLMERLSVDYGKKSKLEF 656
+ LQGF + HS GG G LL+ ++ +Y + F
Sbjct: 117 LDVVRREAEACDALQGFQLTHSLGGGTGS-GMGTLLLSKIREEYPDRMMATF 167
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 31.5 bits (68), Expect = 0.17
Identities = 17/43 (39%), Positives = 22/43 (51%)
Frame = +3
Query: 447 NYARGSLHHWKGNRRFGFGQKSASSLXSVPGLQGFLIFHSFGG 575
++A G+L H +G+ GFG S PG GF F FGG
Sbjct: 173 SHALGNLFHHRGHNGGGFGGFGGGSGGPPPGPGGFGGFGGFGG 215
>SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 622
Score = 27.1 bits (57), Expect = 3.6
Identities = 13/37 (35%), Positives = 17/37 (45%)
Frame = +3
Query: 492 FGFGQKSASSLXSVPGLQGFLIFHSFGGRYRLWGSLP 602
F FG SS ++ + G L SF Y W S+P
Sbjct: 42 FNFGGNVGSSDVTMTSVSGHLTEASFPSEYSSWSSVP 78
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.2
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +1
Query: 223 STASSLMARCPQTRPSGVETILSTLSSARPELAST 327
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC1682.15 |mug122||PX/PXA domain protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 749
Score = 25.8 bits (54), Expect = 8.2
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = -1
Query: 558 RSGILADPVHXSASLRISVQNQIYDFLSN 472
++ +L P SA + ++NQIYD LSN
Sbjct: 589 KTTVLHGPGRFSAQVERMLKNQIYDKLSN 617
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 242 WPDAHRQDHRGWRRFFQHFLQR 307
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,630,143
Number of Sequences: 5004
Number of extensions: 76779
Number of successful extensions: 206
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -