BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP07_F_A14
(831 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 33 0.066
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 31 0.20
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 30 0.35
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 30 0.35
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 28 1.9
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 2.5
SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ... 27 3.3
SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces pomb... 26 5.7
SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit Vps23|Schizosac... 26 7.5
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 25 10.0
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 32.7 bits (71), Expect = 0.066
Identities = 14/27 (51%), Positives = 14/27 (51%)
Frame = +1
Query: 562 PFXGGXFXPPPPKXXPPXXGGXPPPPP 642
P GG PPPP P G PPPPP
Sbjct: 756 PIMGG---PPPPPPPPGVAGAGPPPPP 779
Score = 29.1 bits (62), Expect = 0.81
Identities = 13/34 (38%), Positives = 13/34 (38%)
Frame = +1
Query: 589 PPPKXXPPXXGGXPPPPPXXXXXXXPPXFPXXKP 690
P P P G PPPPP PP P P
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 28.3 bits (60), Expect = 1.4
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +2
Query: 587 PPPQKXXXXXGGGXPPPPP 643
PPP G G PPPPP
Sbjct: 762 PPPPPPPGVAGAGPPPPPP 780
Score = 27.9 bits (59), Expect = 1.9
Identities = 11/24 (45%), Positives = 11/24 (45%)
Frame = +2
Query: 572 GGSXXPPPQKXXXXXGGGXPPPPP 643
GG PPP G PPPPP
Sbjct: 759 GGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 25.8 bits (54), Expect = 7.5
Identities = 16/54 (29%), Positives = 16/54 (29%), Gaps = 4/54 (7%)
Frame = +1
Query: 586 PPPPKXXPPXXG----GXPPPPPXXXXXXXPPXFPXXKPRXPSXXXSXPPTXRA 735
PPPP P PPP P PP P P PP A
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSA 787
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 31.1 bits (67), Expect = 0.20
Identities = 14/46 (30%), Positives = 14/46 (30%)
Frame = +1
Query: 586 PPPPKXXPPXXGGXPPPPPXXXXXXXPPXFPXXKPRXPSXXXSXPP 723
PP P PP PPP PP P P PP
Sbjct: 158 PPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPP 203
Score = 28.7 bits (61), Expect = 1.1
Identities = 17/62 (27%), Positives = 19/62 (30%), Gaps = 3/62 (4%)
Frame = +1
Query: 586 PPPPKXXPPXXGGXPPPP---PXXXXXXXPPXFPXXKPRXPSXXXSXPPTXRAXXXXLXX 756
PP P PP PP P P PP P + P S P +
Sbjct: 144 PPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPP 203
Query: 757 PP 762
PP
Sbjct: 204 PP 205
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 30.3 bits (65), Expect = 0.35
Identities = 19/46 (41%), Positives = 19/46 (41%)
Frame = -2
Query: 722 GGXEXXXEGXRGFXXGXXGGXXXXXXLGGGGGXPPXXGGXXFGGGG 585
GG EG G G GG LGG GG P GG G GG
Sbjct: 227 GGGPGGFEGGPGGFGGGPGGFGGG--LGGFGGGPGGFGGGPGGHGG 270
Score = 28.7 bits (61), Expect = 1.1
Identities = 26/82 (31%), Positives = 27/82 (32%), Gaps = 2/82 (2%)
Frame = -2
Query: 692 RGFXXGXXGGXXXXXXLGGGGGXPPXXGG-XXFGGGGXKXPPXKG-XXXPPPPXFXXXXX 519
RG G GG GG GG PP GG FGG G + G P
Sbjct: 183 RGHNGGGFGGFG-----GGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGP 237
Query: 518 XXXXGGXXXXXXXXGGXGGXXG 453
GG GG GG G
Sbjct: 238 GGFGGGPGGFGGGLGGFGGGPG 259
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 30.3 bits (65), Expect = 0.35
Identities = 16/48 (33%), Positives = 16/48 (33%)
Frame = +1
Query: 547 GGXXXPFXGGXFXPPPPKXXPPXXGGXPPPPPXXXXXXXPPXFPXXKP 690
G P G PPPP P G PPP PP P P
Sbjct: 349 GSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIP 396
Score = 30.3 bits (65), Expect = 0.35
Identities = 13/38 (34%), Positives = 13/38 (34%)
Frame = +1
Query: 586 PPPPKXXPPXXGGXPPPPPXXXXXXXPPXFPXXKPRXP 699
PP PP G P PP PP P P P
Sbjct: 450 PPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAPAAP 487
Score = 26.2 bits (55), Expect = 5.7
Identities = 14/43 (32%), Positives = 14/43 (32%)
Frame = +1
Query: 592 PPKXXPPXXGGXPPPPPXXXXXXXPPXFPXXKPRXPSXXXSXP 720
PP PP PP P PP P P P S P
Sbjct: 415 PPVPTPP---SLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAP 454
Score = 26.2 bits (55), Expect = 5.7
Identities = 14/47 (29%), Positives = 15/47 (31%), Gaps = 2/47 (4%)
Frame = +1
Query: 586 PP--PPKXXPPXXGGXPPPPPXXXXXXXPPXFPXXKPRXPSXXXSXP 720
PP PP P G P PP P P P P + P
Sbjct: 428 PPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAP 474
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 27.9 bits (59), Expect = 1.9
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +1
Query: 586 PPPPKXXPPXXGGXPPPPP 642
PPPP P PPPPP
Sbjct: 12 PPPPPGFEPPSQPPPPPPP 30
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.5 bits (58), Expect = 2.5
Identities = 11/30 (36%), Positives = 11/30 (36%)
Frame = +1
Query: 589 PPPKXXPPXXGGXPPPPPXXXXXXXPPXFP 678
PPP PP G P PP P P
Sbjct: 1715 PPPPSAPPMPAGPPSAPPPPLPASSAPSVP 1744
Score = 25.8 bits (54), Expect = 7.5
Identities = 12/39 (30%), Positives = 13/39 (33%)
Frame = +1
Query: 586 PPPPKXXPPXXGGXPPPPPXXXXXXXPPXFPXXKPRXPS 702
PPPP P PP P PP P P+
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPN 1745
>SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2344
Score = 27.1 bits (57), Expect = 3.3
Identities = 20/78 (25%), Positives = 36/78 (46%), Gaps = 5/78 (6%)
Frame = -1
Query: 306 VEGCXPXGQSFX*QX-----PGPKADSTFSLNSSIVFFALSASVFMLLSSLGASRLTNAC 142
+ GC G+S Q G A + F ++ SIV+ A+ ++V + + A
Sbjct: 389 ITGCEGIGESSLIQTICDRREGYMAITKFEVSQSIVYSAIVSAVAEFIRQILAEDQLLLN 448
Query: 141 TLARQIANKIISSLFILD 88
++ NK+ S L++LD
Sbjct: 449 NFFEELKNKLESDLYLLD 466
>SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 821
Score = 26.2 bits (55), Expect = 5.7
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +2
Query: 170 KEDNSINTLAESAKKTIEELREKVESAL 253
KE NSI+ L+ S +KTIE R + S +
Sbjct: 371 KEKNSISELSPSLQKTIEWARVNLASTI 398
>SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit
Vps23|Schizosaccharomyces pombe|chr 1|||Manual
Length = 487
Score = 25.8 bits (54), Expect = 7.5
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = +2
Query: 140 VHAFVKRDAPKEDNSINTLAESAKKTIEELREKVESALGP 259
V A +K+D +E S L TIE+L+ K E+ P
Sbjct: 128 VQALIKQDFEREHTSPPELPTKLVNTIEKLKVKEENEAPP 167
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 25.4 bits (53), Expect = 10.0
Identities = 17/63 (26%), Positives = 18/63 (28%)
Frame = +1
Query: 574 GXFXPPPPKXXPPXXGGXPPPPPXXXXXXXPPXFPXXKPRXPSXXXSXPPTXRAXXXXLX 753
G PPPP P PPPP P P P PP +
Sbjct: 204 GEHMPPPPMHHKPGE-HMPPPPMHHEPGEHMP--PPPMHHEPGEHMPPPPMHHEPGEHMP 260
Query: 754 XPP 762
PP
Sbjct: 261 PPP 263
Score = 25.4 bits (53), Expect = 10.0
Identities = 17/63 (26%), Positives = 18/63 (28%)
Frame = +1
Query: 574 GXFXPPPPKXXPPXXGGXPPPPPXXXXXXXPPXFPXXKPRXPSXXXSXPPTXRAXXXXLX 753
G PPPP P PPPP P P P PP +
Sbjct: 243 GEHMPPPPMHHEPGE-HMPPPPMHHEPGEHMP--PPPMHHEPGEHMPPPPMHHEPGEHMP 299
Query: 754 XPP 762
PP
Sbjct: 300 PPP 302
Score = 25.4 bits (53), Expect = 10.0
Identities = 17/63 (26%), Positives = 18/63 (28%)
Frame = +1
Query: 574 GXFXPPPPKXXPPXXGGXPPPPPXXXXXXXPPXFPXXKPRXPSXXXSXPPTXRAXXXXLX 753
G PPPP P PPPP P P P PP +
Sbjct: 282 GEHMPPPPMHHEPGE-HMPPPPMHHEPGEHMP--PPPMHHEPGEHMPPPPMHHEPGEHMP 338
Query: 754 XPP 762
PP
Sbjct: 339 PPP 341
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,262,137
Number of Sequences: 5004
Number of extensions: 36859
Number of successful extensions: 227
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 160
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 408446760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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