Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP06_F_P08
(857 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 131 1e-31
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 131 1e-31
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 131 1e-31
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 77 2e-15
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 71 2e-13
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 54 2e-08
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 45 1e-05
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 37 0.003
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 36 0.010
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 36 0.010
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 35 0.017
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 33 0.039
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 30 0.48
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.84
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 29 1.1
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 28 1.5
SPAC139.04c |fap2||L-saccharopine oxidase|Schizosaccharomyces po... 27 2.6
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 27 2.6
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 131 bits (317), Expect = 1e-31
Identities = 59/64 (92%), Positives = 61/64 (95%)
Frame = +2
Query: 104 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 283
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 284 DKTK 295
DK K
Sbjct: 61 DKLK 64
Score = 111 bits (266), Expect = 2e-25
Identities = 50/57 (87%), Positives = 53/57 (92%)
Frame = +3
Query: 288 KLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIXNMITGTSQADCAVLIVAG 458
KLKAERERGITIDIALWKFET KY VT+IDAPGHRDFI NMITGTSQADCA+LI+ G
Sbjct: 62 KLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGG 118
Score = 61.7 bits (143), Expect = 1e-10
Identities = 32/41 (78%), Positives = 35/41 (85%)
Frame = +1
Query: 460 GTGEFEAGISKNXQTREHALLAFHPPVSKQLIVGVNKMDST 582
GTGEFEAGISK+ QTREHALLA+ V KQLIV VNKMD+T
Sbjct: 119 GTGEFEAGISKDGQTREHALLAYTLGV-KQLIVAVNKMDTT 158
Score = 27.1 bits (57), Expect = 3.4
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3
Query: 690 PFSGWXGDNMLEPXT 734
P SG+ GDNM+EP T
Sbjct: 190 PVSGFQGDNMIEPTT 204
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 131 bits (317), Expect = 1e-31
Identities = 59/64 (92%), Positives = 61/64 (95%)
Frame = +2
Query: 104 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 283
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 284 DKTK 295
DK K
Sbjct: 61 DKLK 64
Score = 111 bits (267), Expect = 1e-25
Identities = 51/57 (89%), Positives = 53/57 (92%)
Frame = +3
Query: 288 KLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIXNMITGTSQADCAVLIVAG 458
KLKAERERGITIDIALWKFET KY VT+IDAPGHRDFI NMITGTSQADCAVLI+ G
Sbjct: 62 KLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVLIIGG 118
Score = 61.7 bits (143), Expect = 1e-10
Identities = 32/41 (78%), Positives = 35/41 (85%)
Frame = +1
Query: 460 GTGEFEAGISKNXQTREHALLAFHPPVSKQLIVGVNKMDST 582
GTGEFEAGISK+ QTREHALLA+ V KQLIV VNKMD+T
Sbjct: 119 GTGEFEAGISKDGQTREHALLAYTLGV-KQLIVAVNKMDTT 158
Score = 27.1 bits (57), Expect = 3.4
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3
Query: 690 PFSGWXGDNMLEPXT 734
P SG+ GDNM+EP T
Sbjct: 190 PVSGFQGDNMIEPTT 204
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 131 bits (317), Expect = 1e-31
Identities = 59/64 (92%), Positives = 61/64 (95%)
Frame = +2
Query: 104 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 283
MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEA E+GKGSFKYAWVL
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60
Query: 284 DKTK 295
DK K
Sbjct: 61 DKLK 64
Score = 111 bits (266), Expect = 2e-25
Identities = 50/57 (87%), Positives = 53/57 (92%)
Frame = +3
Query: 288 KLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIXNMITGTSQADCAVLIVAG 458
KLKAERERGITIDIALWKFET KY VT+IDAPGHRDFI NMITGTSQADCA+LI+ G
Sbjct: 62 KLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGG 118
Score = 61.7 bits (143), Expect = 1e-10
Identities = 32/41 (78%), Positives = 35/41 (85%)
Frame = +1
Query: 460 GTGEFEAGISKNXQTREHALLAFHPPVSKQLIVGVNKMDST 582
GTGEFEAGISK+ QTREHALLA+ V KQLIV VNKMD+T
Sbjct: 119 GTGEFEAGISKDGQTREHALLAYTLGV-KQLIVAVNKMDTT 158
Score = 27.1 bits (57), Expect = 3.4
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3
Query: 690 PFSGWXGDNMLEPXT 734
P SG+ GDNM+EP T
Sbjct: 190 PVSGFQGDNMIEPTT 204
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 77.4 bits (182), Expect = 2e-15
Identities = 33/59 (55%), Positives = 45/59 (76%)
Frame = +2
Query: 116 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKT 292
K H+NIV IGHVD+GKST G++++ G +DKRT+EK E+EA+E GK S+ +W LD T
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDST 294
Score = 59.3 bits (137), Expect = 7e-10
Identities = 24/54 (44%), Positives = 37/54 (68%)
Frame = +3
Query: 300 ERERGITIDIALWKFETSKYYVTIIDAPGHRDFIXNMITGTSQADCAVLIVAGR 461
ERE+G T+++ FET +++DAPGH+ ++ NMI G SQAD VL+++ R
Sbjct: 297 EREKGKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISAR 350
Score = 41.9 bits (94), Expect = 1e-04
Identities = 23/46 (50%), Positives = 30/46 (65%)
Frame = +1
Query: 457 AGTGEFEAGISKNXQTREHALLAFHPPVSKQLIVGVNKMDSTEPXI 594
A GEFEAG + QTREHA+LA ++ L+V +NKMD EP +
Sbjct: 349 ARRGEFEAGFERGGQTREHAVLARTQGIN-HLVVVINKMD--EPSV 391
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 71.3 bits (167), Expect = 2e-13
Identities = 30/60 (50%), Positives = 43/60 (71%)
Frame = +2
Query: 116 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKTK 295
K +++VV GHVDSGKST G ++++ G I+ R+++K EA GKGSF YAW+LD T+
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234
Score = 62.9 bits (146), Expect = 6e-11
Identities = 30/51 (58%), Positives = 35/51 (68%)
Frame = +3
Query: 300 ERERGITIDIALWKFETSKYYVTIIDAPGHRDFIXNMITGTSQADCAVLIV 452
ER RG+T+D+A FE+ K I DAPGHRDFI MI G S AD AVL+V
Sbjct: 236 ERARGVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLVV 286
Score = 33.9 bits (74), Expect = 0.030
Identities = 17/35 (48%), Positives = 23/35 (65%)
Frame = +1
Query: 472 FEAGISKNXQTREHALLAFHPPVSKQLIVGVNKMD 576
FE G +N QTREHA L +S +++V VNK+D
Sbjct: 293 FERGFLENGQTREHAYLLRALGIS-EIVVSVNKLD 326
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 54.4 bits (125), Expect = 2e-08
Identities = 24/56 (42%), Positives = 36/56 (64%)
Frame = +3
Query: 288 KLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIXNMITGTSQADCAVLIVA 455
K E+ RGITI A ++ET+ + +D PGH D+I NMITG + D A+++V+
Sbjct: 93 KAPEEKARGITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAATMDGAIIVVS 148
Score = 35.1 bits (77), Expect = 0.013
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +2
Query: 110 KEKTHINIVVIGHVDSGKSTTTGHLIYKC 196
++K H+NI IGHVD GK+T T I KC
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC 76
Score = 33.5 bits (73), Expect = 0.039
Identities = 17/30 (56%), Positives = 21/30 (70%)
Frame = +1
Query: 499 QTREHALLAFHPPVSKQLIVGVNKMDSTEP 588
QTREH LLA V KQ++V +NK+D EP
Sbjct: 156 QTREHLLLARQVGV-KQIVVYINKVDMVEP 184
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 45.2 bits (102), Expect = 1e-05
Identities = 23/56 (41%), Positives = 30/56 (53%)
Frame = +3
Query: 291 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIXNMITGTSQADCAVLIVAG 458
L AER+RGITI+ A F + +ID PGH DF + + D AV I+ G
Sbjct: 71 LPAERQRGITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDG 126
Score = 28.7 bits (61), Expect = 1.1
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +2
Query: 128 NIVVIGHVDSGKSTTTGHLIYKCG 199
N+ +I H+D+GK+T T ++Y G
Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG 53
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 37.1 bits (82), Expect = 0.003
Identities = 18/59 (30%), Positives = 31/59 (52%), Gaps = 4/59 (6%)
Frame = +3
Query: 288 KLKAERERGITIDIA----LWKFETSKYYVTIIDAPGHRDFIXNMITGTSQADCAVLIV 452
KL+ ER RGIT+ ++ + Y + +ID PGH DF ++ + + +L+V
Sbjct: 97 KLEVERRRGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLV 155
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.010
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +2
Query: 104 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 199
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 27.5 bits (58), Expect = 2.6
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +3
Query: 357 YYVTIIDAPGHRDFIXNMITGTSQADCAVLIV 452
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 35.5 bits (78), Expect = 0.010
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = +2
Query: 104 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCG 199
MGK N+ VI HVD GKST T L+ K G
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAG 44
Score = 27.5 bits (58), Expect = 2.6
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +3
Query: 357 YYVTIIDAPGHRDFIXNMITGTSQADCAVLIV 452
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 34.7 bits (76), Expect = 0.017
Identities = 19/51 (37%), Positives = 26/51 (50%), Gaps = 2/51 (3%)
Frame = +3
Query: 312 GITIDIALWK--FETSKYYVTIIDAPGHRDFIXNMITGTSQADCAVLIVAG 458
GIT I + F+ ++T +D PGH F G + AD VL+VAG
Sbjct: 203 GITQKIGAFTVPFDKGSKFITFLDTPGHMAFEAMRKRGANIADIVVLVVAG 253
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 33.5 bits (73), Expect = 0.039
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = +3
Query: 360 YVTIIDAPGHRDFIXNMITGTSQADCAVLIVAG 458
+V+ +D PGH + M+ G + D A+L++AG
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLIAG 141
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 29.9 bits (64), Expect = 0.48
Identities = 16/37 (43%), Positives = 20/37 (54%)
Frame = +3
Query: 342 FETSKYYVTIIDAPGHRDFIXNMITGTSQADCAVLIV 452
FE S Y + IID PGH DF + D AVL++
Sbjct: 141 FEKS-YNINIIDTPGHIDFTIEVERALRVLDGAVLVL 176
Score = 29.1 bits (62), Expect = 0.84
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +2
Query: 128 NIVVIGHVDSGKSTTTGHLIYKCGGI 205
NI + H+DSGK+T T ++Y G I
Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI 86
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.1 bits (62), Expect = 0.84
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 369 IIDAPGHRDFIXNMITGTSQADCAVLIV 452
IID PGH F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 28.7 bits (61), Expect = 1.1
Identities = 15/43 (34%), Positives = 26/43 (60%), Gaps = 3/43 (6%)
Frame = +3
Query: 288 KLKAER-ERGITIDIALWKFETSKYYV--TIIDAPGHRDFIXN 407
K++A+ E+ + I+I + E +++ T+ID PG DFI N
Sbjct: 61 KVRAKHAEKTVEIEITKAELEEKNFHLRLTVIDTPGFGDFINN 103
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 28.3 bits (60), Expect = 1.5
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +3
Query: 357 YYVTIIDAPGHRDFIXNMITGTSQADCAVLIV 452
Y + +ID+PGH DF + + + D A ++V
Sbjct: 94 YLINLIDSPGHVDFSSEVSSASRLCDGAFVLV 125
>SPAC139.04c |fap2||L-saccharopine oxidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 433
Score = 27.5 bits (58), Expect = 2.6
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = -1
Query: 101 LVSHGLQTACNIRDRLQQNQKILRIPYS 18
L S G + CN+ +N K++ IP+S
Sbjct: 280 LASTGYEYVCNVETDYDENSKVVSIPHS 307
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 27.5 bits (58), Expect = 2.6
Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 5/56 (8%)
Frame = +3
Query: 300 ERERGITID-----IALWKFETSKYYVTIIDAPGHRDFIXNMITGTSQADCAVLIV 452
ERER ++I +A+ + + ID PGH DF+ + + +D VL+V
Sbjct: 185 ERERVMSIKSTPLTLAVSDMKGKTFAFQCIDTPGHVDFVDEVAAPMAISDGVVLVV 240
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,241,829
Number of Sequences: 5004
Number of extensions: 64673
Number of successful extensions: 180
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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