Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP06_F_P08
(857 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 136 3e-34
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 136 3e-34
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 125 5e-31
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 102 4e-24
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 29 0.041
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.6
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 3.6
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 136 bits (328), Expect = 3e-34
Identities = 62/64 (96%), Positives = 62/64 (96%)
Frame = +2
Query: 104 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 283
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 284 DKTK 295
DK K
Sbjct: 61 DKLK 64
Score = 116 bits (278), Expect = 4e-28
Identities = 54/56 (96%), Positives = 55/56 (98%)
Frame = +3
Query: 288 KLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIXNMITGTSQADCAVLIVA 455
KLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFI NMITGTSQADCAVLIVA
Sbjct: 62 KLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 117
Score = 69.3 bits (162), Expect = 4e-14
Identities = 36/44 (81%), Positives = 37/44 (84%)
Frame = +1
Query: 457 AGTGEFEAGISKNXQTREHALLAFHPPVSKQLIVGVNKMDSTEP 588
AG GEFEAGISKN QTREHALLAF V KQLIVGVNKMD T+P
Sbjct: 118 AGIGEFEAGISKNGQTREHALLAFTLGV-KQLIVGVNKMDMTDP 160
Score = 37.1 bits (82), Expect = 2e-04
Identities = 25/55 (45%), Positives = 28/55 (50%)
Frame = +3
Query: 690 PFSGWXGDNMLEPXTPNALGXXDXRWTPKEGXPDGKCXP*XLSDAILATWPAPTD 854
P SGW GDNMLEP +P + K+G DGK L DAIL PTD
Sbjct: 192 PISGWHGDNMLEP-SPKTPWYKGWKVERKDGNADGKTLIEAL-DAILPP-SRPTD 243
Score = 34.3 bits (75), Expect = 0.001
Identities = 19/37 (51%), Positives = 24/37 (64%)
Frame = +2
Query: 584 NPPYREPRFEENQERKYXSYXKKIGYXPACXSPXVPI 694
+PPY E RFEE +++ SY KKIGY A + VPI
Sbjct: 159 DPPYSEARFEE-IKKEVSSYIKKIGYNTASVA-FVPI 193
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 136 bits (328), Expect = 3e-34
Identities = 62/64 (96%), Positives = 62/64 (96%)
Frame = +2
Query: 104 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 283
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 284 DKTK 295
DK K
Sbjct: 61 DKLK 64
Score = 125 bits (302), Expect = 5e-31
Identities = 91/191 (47%), Positives = 99/191 (51%), Gaps = 2/191 (1%)
Frame = +3
Query: 288 KLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIXNMITGTSQADCAVLIVAGRYR 467
KLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFI NMITGTSQADCAVLIVA
Sbjct: 62 KLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 121
Query: 468 *IRSWYL*EXSNP*ACLARLSPSGVQTAHRRSKQNGFH*TXHTVSPDLRKIKKGSXPHTS 647
+ L + Q +K + +IKK +
Sbjct: 122 EFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETR--FEEIKKEVSSYI- 178
Query: 648 RKLATXXPAGXLXCPFSGWXGDNMLEPXT--PNALGXXDXRWTPKEGXPDGKCXP*XLSD 821
K PA P SGW GDNMLE + P G R KEG +GKC L D
Sbjct: 179 -KKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVER---KEGKVEGKCLIEAL-D 233
Query: 822 AILATWPAPTD 854
AIL PTD
Sbjct: 234 AILPP-TRPTD 243
Score = 74.9 bits (176), Expect = 8e-16
Identities = 39/44 (88%), Positives = 39/44 (88%)
Frame = +1
Query: 457 AGTGEFEAGISKNXQTREHALLAFHPPVSKQLIVGVNKMDSTEP 588
AGTGEFEAGISKN QTREHALLAF V KQLIVGVNKMDSTEP
Sbjct: 118 AGTGEFEAGISKNGQTREHALLAFTLGV-KQLIVGVNKMDSTEP 160
Score = 37.5 bits (83), Expect = 2e-04
Identities = 20/36 (55%), Positives = 24/36 (66%)
Frame = +2
Query: 587 PPYREPRFEENQERKYXSYXKKIGYXPACXSPXVPI 694
PPY E RFEE + ++ SY KKIGY PA + VPI
Sbjct: 160 PPYSETRFEEIK-KEVSSYIKKIGYNPAAVA-FVPI 193
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 125 bits (302), Expect = 5e-31
Identities = 91/191 (47%), Positives = 99/191 (51%), Gaps = 2/191 (1%)
Frame = +3
Query: 288 KLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIXNMITGTSQADCAVLIVAGRYR 467
KLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFI NMITGTSQADCAVLIVA
Sbjct: 5 KLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 64
Query: 468 *IRSWYL*EXSNP*ACLARLSPSGVQTAHRRSKQNGFH*TXHTVSPDLRKIKKGSXPHTS 647
+ L + Q +K + +IKK +
Sbjct: 65 EFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETR--FEEIKKEVSSYI- 121
Query: 648 RKLATXXPAGXLXCPFSGWXGDNMLEPXT--PNALGXXDXRWTPKEGXPDGKCXP*XLSD 821
K PA P SGW GDNMLE + P G R KEG +GKC L D
Sbjct: 122 -KKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVER---KEGKVEGKCLIEAL-D 176
Query: 822 AILATWPAPTD 854
AIL PTD
Sbjct: 177 AILPP-TRPTD 186
Score = 74.9 bits (176), Expect = 8e-16
Identities = 39/44 (88%), Positives = 39/44 (88%)
Frame = +1
Query: 457 AGTGEFEAGISKNXQTREHALLAFHPPVSKQLIVGVNKMDSTEP 588
AGTGEFEAGISKN QTREHALLAF V KQLIVGVNKMDSTEP
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGV-KQLIVGVNKMDSTEP 103
Score = 37.5 bits (83), Expect = 2e-04
Identities = 20/36 (55%), Positives = 24/36 (66%)
Frame = +2
Query: 587 PPYREPRFEENQERKYXSYXKKIGYXPACXSPXVPI 694
PPY E RFEE + ++ SY KKIGY PA + VPI
Sbjct: 103 PPYSETRFEEIK-KEVSSYIKKIGYNPAAVA-FVPI 136
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 102 bits (245), Expect = 4e-24
Identities = 79/179 (44%), Positives = 87/179 (48%), Gaps = 2/179 (1%)
Frame = +3
Query: 324 DIALWKFETSKYYVTIIDAPGHRDFIXNMITGTSQADCAVLIVAGRYR*IRSWYL*EXSN 503
DIALWKFETSKYYVTIIDAPGHRDFI NMITGTSQADCAVLIVA +
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 504 P*ACLARLSPSGVQTAHRRSKQNGFH*TXHTVSPDLRKIKKGSXPHTSRKLATXXPAGXL 683
L + Q +K + +IKK + K PA
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETR--FEEIKKEVSSYI--KKIGYNPAAVA 116
Query: 684 XCPFSGWXGDNMLEPXT--PNALGXXDXRWTPKEGXPDGKCXP*XLSDAILATWPAPTD 854
P SGW GDNMLE + P G R KEG +GKC L DAIL PTD
Sbjct: 117 FVPISGWHGDNMLEVSSKMPWFKGWTVER---KEGKVEGKCLIEAL-DAILPP-TRPTD 170
Score = 74.9 bits (176), Expect = 8e-16
Identities = 39/44 (88%), Positives = 39/44 (88%)
Frame = +1
Query: 457 AGTGEFEAGISKNXQTREHALLAFHPPVSKQLIVGVNKMDSTEP 588
AGTGEFEAGISKN QTREHALLAF V KQLIVGVNKMDSTEP
Sbjct: 45 AGTGEFEAGISKNGQTREHALLAFTLGV-KQLIVGVNKMDSTEP 87
Score = 37.5 bits (83), Expect = 2e-04
Identities = 20/36 (55%), Positives = 24/36 (66%)
Frame = +2
Query: 587 PPYREPRFEENQERKYXSYXKKIGYXPACXSPXVPI 694
PPY E RFEE + ++ SY KKIGY PA + VPI
Sbjct: 87 PPYSETRFEEIK-KEVSSYIKKIGYNPAAVA-FVPI 120
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 29.5 bits (63), Expect = 0.041
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = +3
Query: 363 VTIIDAPGHRDFIXNMITGTSQADCAVLIVA 455
VT +D PGH FI G D VL+VA
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVA 225
Score = 25.4 bits (53), Expect = 0.68
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +2
Query: 116 KTHINIVVIGHVDSGKST 169
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.6
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +2
Query: 125 INIVVIGHVDSGKST 169
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 3.6
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -3
Query: 117 FSLPIFG*SRITNCV*Y 67
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 223,077
Number of Sequences: 438
Number of extensions: 4676
Number of successful extensions: 33
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 27673956
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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