BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP06_F_O18
(999 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 30 0.44
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 30 0.44
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 5.5
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 5.5
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 7.2
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 23 9.1
SPAC26F1.08c |||conserved protein|Schizosaccharomyces pombe|chr ... 26 9.5
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 30.3 bits (65), Expect = 0.44
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = +2
Query: 947 PPPPPPXXXPPXSAPP 994
PPPPP PP SAPP
Sbjct: 1707 PPPPPMSVPPPPSAPP 1722
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 30.3 bits (65), Expect = 0.44
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = +2
Query: 947 PPPPPPXXXPPXSAPP 994
PPPPPP PP PP
Sbjct: 11 PPPPPPGFEPPSQPPP 26
Score = 29.5 bits (63), Expect = 0.78
Identities = 12/23 (52%), Positives = 12/23 (52%)
Frame = +2
Query: 926 SLSXPXXPPPPPPXXXPPXSAPP 994
SL PPPPPP P S PP
Sbjct: 3 SLPPGNPPPPPPPPGFEPPSQPP 25
Score = 29.1 bits (62), Expect = 1.0
Identities = 11/22 (50%), Positives = 11/22 (50%)
Frame = +1
Query: 931 LXPXXPPPPPPXXXSXPXXAPP 996
L P PPPPPP P PP
Sbjct: 4 LPPGNPPPPPPPPGFEPPSQPP 25
Score = 26.6 bits (56), Expect = 5.5
Identities = 9/17 (52%), Positives = 9/17 (52%)
Frame = +1
Query: 946 PPPPPPXXXSXPXXAPP 996
PPPPPP P PP
Sbjct: 10 PPPPPPPGFEPPSQPPP 26
Score = 25.8 bits (54), Expect = 9.5
Identities = 11/22 (50%), Positives = 11/22 (50%), Gaps = 3/22 (13%)
Frame = +2
Query: 938 PXXPPPPP---PXXXPPXSAPP 994
P PPPPP P PP PP
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPP 30
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.6 bits (56), Expect = 5.5
Identities = 9/13 (69%), Positives = 9/13 (69%)
Frame = +2
Query: 947 PPPPPPXXXPPXS 985
PPPPPP PP S
Sbjct: 238 PPPPPPPTLPPQS 250
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.6 bits (56), Expect = 5.5
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = +2
Query: 947 PPPPPPXXXPPXSAP 991
PPPPPP P +AP
Sbjct: 5 PPPPPPAPAPAAAAP 19
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 26.2 bits (55), Expect = 7.2
Identities = 13/25 (52%), Positives = 13/25 (52%), Gaps = 3/25 (12%)
Frame = +2
Query: 929 LSXPXXPPPPPPXXXP---PXSAPP 994
LS PPPPPP P P SA P
Sbjct: 1876 LSNQAAPPPPPPMALPKAGPPSAAP 1900
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 23.4 bits (48), Expect(2) = 9.1
Identities = 8/13 (61%), Positives = 8/13 (61%)
Frame = +2
Query: 947 PPPPPPXXXPPXS 985
PPPPPP P S
Sbjct: 240 PPPPPPRPSQPTS 252
Score = 20.6 bits (41), Expect(2) = 9.1
Identities = 8/14 (57%), Positives = 8/14 (57%)
Frame = +2
Query: 923 FSLSXPXXPPPPPP 964
FS S PPPP P
Sbjct: 234 FSSSSTPPPPPPRP 247
>SPAC26F1.08c |||conserved protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 977
Score = 25.8 bits (54), Expect = 9.5
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = +1
Query: 49 KNLFLTIPGLQAXRXPGPKVSALKXNL 129
KNL L + GL+A R PK+ K NL
Sbjct: 471 KNLLLLLEGLKAQRHNKPKLFFRKFNL 497
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,410,884
Number of Sequences: 5004
Number of extensions: 14358
Number of successful extensions: 262
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 40
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 517271174
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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