Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP06_F_M10
(880 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 217 2e-57
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 115 8e-27
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 96 5e-21
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 81 3e-16
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 50 3e-07
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 47 3e-06
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 42 9e-05
SPCC1020.10 |oca2||serine/threonine protein kinase Oca2 |Schizos... 28 2.0
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 27 4.7
SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosacc... 26 8.1
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc... 26 8.1
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 26 8.1
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 217 bits (530), Expect = 2e-57
Identities = 109/166 (65%), Positives = 119/166 (71%)
Frame = +2
Query: 245 PAIRGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVA 424
P G+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVA
Sbjct: 38 PRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVA 97
Query: 425 PEEHPVLLTEAPLNPRXTERR*PRSCSKHSTRPPCTSPSKPCSRCTRPVRTTGIVLDSGE 604
PEEHP LLTEAPLNP+ + + + P + RTTGIVLDSG+
Sbjct: 98 PEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGD 157
Query: 605 GVSHTVXIYEGYALPPRHPGSGTWAGRDLXDYXMKILXERG*SFTT 742
GV+HTV IYEGYALP AGRDL DY MKIL ERG +F+T
Sbjct: 158 GVTHTVPIYEGYALPHAIMRLDL-AGRDLTDYLMKILMERGYTFST 202
Score = 127 bits (307), Expect = 2e-30
Identities = 81/156 (51%), Positives = 91/156 (58%), Gaps = 11/156 (7%)
Frame = +1
Query: 136 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG-------RDGRYGTEGLVR 294
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G +D G E +
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 295 R*RGTEQKRYPDPQIPHRTRNRH*LG*HGEDLASY----LLQ*AACRPRGTPRPAH*GSP 462
RG +YP I H N + +D+ P P
Sbjct: 62 --RGILTLKYP---IEHGIVN------NWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPL 110
Query: 463 QPKXNREKMTQIMFETFNTPAMYVAIQAVLSLYASG 570
PK NREKMTQI+FETFN PA YVAIQAVLSLYASG
Sbjct: 111 NPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASG 146
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 115 bits (277), Expect = 8e-27
Identities = 61/157 (38%), Positives = 89/157 (56%), Gaps = 1/157 (0%)
Frame = +2
Query: 260 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEH 436
VM QKD +VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 437 PVLLTEAPLNPRXTERR*PRSCSKHSTRPPCTSPSKPCSRCTRPVRTTGIVLDSGEGVSH 616
P+LLTE PL + + + P + +P RTTGIVL+ G+G++H
Sbjct: 107 PLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTH 166
Query: 617 TVXIYEGYALPPRHPGSGTWAGRDLXDYXMKILXERG 727
+V IY+G+++P GRD+ DY L + G
Sbjct: 167 SVPIYDGFSIPSAIQQEEI-GGRDVTDYLQLQLRKSG 202
Score = 55.2 bits (127), Expect = 1e-08
Identities = 46/148 (31%), Positives = 70/148 (47%), Gaps = 10/148 (6%)
Frame = +1
Query: 154 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ-------GRDGRYGTEGLVRR*RGTE 312
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+ +D G+E + RG
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSE--AQNLRGLL 69
Query: 313 QKRYP-DPQIPHRTRNRH*LG*HGEDLASYLLQ*AACRPRGTPRPAH*GSPQPKXNREK- 486
+ + P + I + E++ SY+ P P R K
Sbjct: 70 KIQRPIERGIIQNWSDM-------EEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKE 122
Query: 487 -MTQIMFETFNTPAMYVAIQAVLSLYAS 567
+ + +ET N PA+ ++Q VL+LYAS
Sbjct: 123 KIAEYFYETLNVPALSFSLQPVLALYAS 150
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 96.3 bits (229), Expect = 5e-21
Identities = 51/153 (33%), Positives = 88/153 (57%), Gaps = 1/153 (0%)
Frame = +2
Query: 281 KDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 460
KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 461 LNP-RXTERR*PRSCSKHSTRPPCTSPSKPCSRCTRPVRTTGIVLDSGEGVSHTVXIYEG 637
+NP E+ ++ + S + + ++G+V+DSG+GV+H V +YE
Sbjct: 110 MNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGL-SSGVVVDSGDGVTHIVPVYES 168
Query: 638 YALPPRHPGSGTWAGRDLXDYXMKILXERG*SF 736
L G AGRD Y + +L +G +F
Sbjct: 169 VVL-NHLVGRLDVAGRDATRYLISLLLRKGYAF 200
Score = 67.7 bits (158), Expect = 2e-12
Identities = 49/145 (33%), Positives = 66/145 (45%), Gaps = 2/145 (1%)
Frame = +1
Query: 142 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGRDGRYGTEGLVRR*RGTEQ 315
E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + G + ++
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61
Query: 316 KRYPDPQIPHRTRNRH*LG*HGEDLASYLLQ*AACRPRGTPRPAH*GSPQPKXNREKMTQ 495
+ + P + + PRG P NREKM +
Sbjct: 62 RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCE 121
Query: 496 IMFETFNTPAMYVAIQAVLSLYASG 570
MFE + +YVAIQAVLSLYA G
Sbjct: 122 TMFERYGFGGVYVAIQAVLSLYAQG 146
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 80.6 bits (190), Expect = 3e-16
Identities = 49/156 (31%), Positives = 75/156 (48%), Gaps = 9/156 (5%)
Frame = +2
Query: 284 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 460
D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE P
Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132
Query: 461 LNPRXTERR*PR------SCS--KHSTRPPCTSPSKPCSRCTRPVRTTGIVLDSGEGVSH 616
LNP +C+ + + + S TG V+DSG+GV+H
Sbjct: 133 LNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTH 192
Query: 617 TVXIYEGYALPPRHPGSGTWAGRDLXDYXMKILXER 724
+ + EGY + + AGRD+ + +L +R
Sbjct: 193 IIPVAEGYVIGSSIK-TMPLAGRDVTYFVQSLLRDR 227
Score = 44.4 bits (100), Expect = 2e-05
Identities = 19/34 (55%), Positives = 25/34 (73%)
Frame = +1
Query: 466 PKXNREKMTQIMFETFNTPAMYVAIQAVLSLYAS 567
P NRE +IMFE+FN +Y+A+QAVL+L AS
Sbjct: 135 PPENRENTAEIMFESFNCAGLYIAVQAVLALAAS 168
Score = 40.7 bits (91), Expect = 3e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +1
Query: 154 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 237
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 50.4 bits (115), Expect = 3e-07
Identities = 48/148 (32%), Positives = 66/148 (44%), Gaps = 4/148 (2%)
Frame = +1
Query: 139 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGRDGR--YGTEGLVRR*RGTE 312
+EV+A+V+D GS + GF+G+D P+ V PS G + DGR +G E +
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG----EFSDGRRLFGEEYI-------- 56
Query: 313 QKRYPDPQIPHRTRNRH*LG*H-GEDLASY-LLQ*AACRPRGTPRPAH*GSPQPKXNREK 486
K P +I + RN DL Y L Q P P P NR K
Sbjct: 57 YKSNPGMEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVK 116
Query: 487 MTQIMFETFNTPAMYVAIQAVLSLYASG 570
+ MFE+ PA Y+A Q + +ASG
Sbjct: 117 TLETMFESLRCPATYLAKQETCAAFASG 144
Score = 46.8 bits (106), Expect = 4e-06
Identities = 31/109 (28%), Positives = 49/109 (44%), Gaps = 2/109 (1%)
Frame = +2
Query: 326 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPRXTERR*PRSCS 505
+ +K I +G V NWD +W + +L+ P EHP+L+TE P + R
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITE-PFDNPPENRVKTLETM 121
Query: 506 KHSTRPPCT--SPSKPCSRCTRPVRTTGIVLDSGEGVSHTVXIYEGYAL 646
S R P T + + C+ + T ++D G S IY+G+ L
Sbjct: 122 FESLRCPATYLAKQETCAAFASG-KGTACLVDIGAERSSVSAIYDGFVL 169
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 47.2 bits (107), Expect = 3e-06
Identities = 37/159 (23%), Positives = 68/159 (42%), Gaps = 4/159 (2%)
Frame = +2
Query: 272 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL 448
+ + + VG++ + G ++ + P E +++NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 449 TEAPLNPRXTERR*PRSCSKHSTRPPCT---SPSKPCSRCTRPVRTTGIVLDSGEGVSHT 619
TE NP + P T+P ++GIVL+ G SH
Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKP-SSSGIVLNLGNAASHV 179
Query: 620 VXIYEGYALPPRHPGSGTWAGRDLXDYXMKILXERG*SF 736
+ + G + +W G Y +K+ + SF
Sbjct: 180 IPVLNGERILSEAKRI-SWGGSQSSSYLLKLFQIKYPSF 217
Score = 35.9 bits (79), Expect = 0.008
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +1
Query: 154 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 249
LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
Score = 29.5 bits (63), Expect = 0.66
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +1
Query: 466 PKXNREKMTQIMFETFNTPAMYVAIQAVLSLY 561
P R MT+++FE +N P++ I + S Y
Sbjct: 127 PTYVRSTMTELLFELYNAPSVAYGIDGLFSFY 158
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 42.3 bits (95), Expect = 9e-05
Identities = 27/133 (20%), Positives = 57/133 (42%), Gaps = 2/133 (1%)
Frame = +2
Query: 245 PAIRGVMVGMGQKDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELR 418
P+ GV + ++ YV DE Q I + +K +GI+ +W+ W +L+
Sbjct: 37 PSYYGVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQ 96
Query: 419 VAPEEHPVLLTEAPLNPRXTERR*PRSCSKHSTRPPCTSPSKPCSRCTRPVRTTGIVLDS 598
V P E+ +++TE NP+ ++ + + P + ++T +++D
Sbjct: 97 VNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDI 156
Query: 599 GEGVSHTVXIYEG 637
G + + +G
Sbjct: 157 GSDNASVTPVVDG 169
Score = 36.7 bits (81), Expect = 0.004
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +1
Query: 139 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 240
EE+ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPCC1020.10 |oca2||serine/threonine protein kinase Oca2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 650
Score = 27.9 bits (59), Expect = 2.0
Identities = 14/55 (25%), Positives = 26/55 (47%), Gaps = 1/55 (1%)
Frame = +3
Query: 396 IPSTMSCVSPPRNTPSCSLRLPSTQGQQREDDPDH-VRNIQHARHVRRHPSRALA 557
+ + + SPPRN P + ++ +DD +H + +Q H++ H LA
Sbjct: 31 LDTELKITSPPRNPPQRLHPVDFSEHADTDDDMNHPLPRVQSPVHIKNHIDPKLA 85
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 26.6 bits (56), Expect = 4.7
Identities = 16/43 (37%), Positives = 20/43 (46%), Gaps = 2/43 (4%)
Frame = +2
Query: 401 FYNELRVAPEEHPVLLTE--APLNPRXTERR*PRSCSKHSTRP 523
+Y L E+HP+LLT+ A L PR R R K P
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441
>SPCP1E11.04c |pal1||membrane associated protein Pal1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 425
Score = 25.8 bits (54), Expect = 8.1
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -1
Query: 193 NRPCTYRSHCLLPTRQLP 140
NRP YRSH + TR+ P
Sbjct: 323 NRPSNYRSHSSMGTRRSP 340
>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 721
Score = 25.8 bits (54), Expect = 8.1
Identities = 7/18 (38%), Positives = 13/18 (72%)
Frame = +2
Query: 596 SGEGVSHTVXIYEGYALP 649
+G G+ H + + +GYA+P
Sbjct: 157 AGRGIGHQIMVEQGYAMP 174
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 25.8 bits (54), Expect = 8.1
Identities = 7/21 (33%), Positives = 13/21 (61%)
Frame = +2
Query: 341 PIEHGIVTNWDDMEKIWHHTF 403
PI+ G V +W+ ++ W H +
Sbjct: 113 PIQRGRVVDWEALKAFWKHLY 133
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,588,437
Number of Sequences: 5004
Number of extensions: 75935
Number of successful extensions: 251
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 210
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 243
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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