Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP06_F_L14
(966 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 33 0.060
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.24
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 31 0.24
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 4.0
SPBC1289.16c ||SPBC8E4.06|copper amine oxidase |Schizosaccharomy... 27 5.2
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 9.1
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 33.1 bits (72), Expect = 0.060
Identities = 16/53 (30%), Positives = 20/53 (37%)
Frame = +2
Query: 368 PPXXXPLSPXXXAPPPXQXXQVLXPXVPXFXXPXPXPXXPLPLPXXAXPXPPL 526
PP P + PPP + V P V P P P+P P P P+
Sbjct: 1179 PPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPV 1231
Score = 32.3 bits (70), Expect = 0.11
Identities = 20/71 (28%), Positives = 22/71 (30%)
Frame = +2
Query: 311 PXXPTSGGXMKXGPXTXAPPPXXXPLSPXXXAPPPXQXXQVLXPXVPXFXXPXPXPXXPL 490
P P G + A PP P P P V P V P P P+
Sbjct: 1083 PPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPV 1142
Query: 491 PLPXXAXPXPP 523
P P A P P
Sbjct: 1143 PKPSVAAPPVP 1153
Score = 29.5 bits (63), Expect = 0.74
Identities = 19/71 (26%), Positives = 21/71 (29%)
Frame = +2
Query: 311 PXXPTSGGXMKXGPXTXAPPPXXXPLSPXXXAPPPXQXXQVLXPXVPXFXXPXPXPXXPL 490
P P + P PP P P P V P V P P P+
Sbjct: 1102 PPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPV 1161
Query: 491 PLPXXAXPXPP 523
P P A P P
Sbjct: 1162 PKPSVAAPPVP 1172
Score = 29.1 bits (62), Expect = 0.98
Identities = 21/73 (28%), Positives = 24/73 (32%), Gaps = 3/73 (4%)
Frame = +2
Query: 311 PXXPTSGGXMKXGPXTXAPPPXXXPLSPXXXAPPPXQXXQVLXPXVPXFXXPXPXP---X 481
P P G + A PP P S P P + P P P P P
Sbjct: 1150 PPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPP--PSEAPPVPKPSVGV 1207
Query: 482 XPLPLPXXAXPXP 520
P+P P A P P
Sbjct: 1208 PPVPPPSTAPPVP 1220
Score = 28.3 bits (60), Expect = 1.7
Identities = 17/61 (27%), Positives = 22/61 (36%), Gaps = 2/61 (3%)
Frame = +2
Query: 350 PXTXAPPPXXXPLSPXXXAPPPXQXXQVLXPXVPX--FXXPXPXPXXPLPLPXXAXPXPP 523
P + PP S APPP P +P P P P P+P + PP
Sbjct: 1044 PIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPP 1103
Query: 524 L 526
+
Sbjct: 1104 V 1104
Score = 28.3 bits (60), Expect = 1.7
Identities = 18/72 (25%), Positives = 21/72 (29%)
Frame = +2
Query: 311 PXXPTSGGXMKXGPXTXAPPPXXXPLSPXXXAPPPXQXXQVLXPXVPXFXXPXPXPXXPL 490
P P + P PP P P V P V P P P+
Sbjct: 1064 PPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPV 1123
Query: 491 PLPXXAXPXPPL 526
P P A P P+
Sbjct: 1124 PKPSVAAPPVPV 1135
Score = 26.6 bits (56), Expect = 5.2
Identities = 19/74 (25%), Positives = 27/74 (36%), Gaps = 5/74 (6%)
Frame = +2
Query: 320 PTSGGXMKXGPXTXAPPPXXXPLS-PXXXAPPPXQXXQVLXPXVPXFXXPXPXPXXPL-- 490
P++ G GP APP PL + P + + P P P PL
Sbjct: 951 PSNDGRKASGPRPAAPPSIPPPLPVSNILSSPTSEPPKDHPPSAPLSKPVSTSPAAPLAR 1010
Query: 491 --PLPXXAXPXPPL 526
P+P + PP+
Sbjct: 1011 VPPVPKLSSKAPPV 1024
Score = 26.6 bits (56), Expect = 5.2
Identities = 18/73 (24%), Positives = 22/73 (30%)
Frame = +2
Query: 311 PXXPTSGGXMKXGPXTXAPPPXXXPLSPXXXAPPPXQXXQVLXPXVPXFXXPXPXPXXPL 490
P P + P PP P PPP V P P P P
Sbjct: 1179 PPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAP- 1237
Query: 491 PLPXXAXPXPPLS 529
P+P + P +S
Sbjct: 1238 PVPAPSSEAPSVS 1250
Score = 26.2 bits (55), Expect = 6.9
Identities = 20/66 (30%), Positives = 23/66 (34%), Gaps = 4/66 (6%)
Frame = +2
Query: 350 PXTXAPP---PXXXPLSPXXXAPPPXQXXQVLXPXVPXFXXPXPXPXXP-LPLPXXAXPX 517
P APP P P P + PP P P P P P +P P A P
Sbjct: 1027 PSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPP-PVPAPSSEIPSIPAPSGAPPV 1085
Query: 518 PPLSXL 535
P S +
Sbjct: 1086 PAPSGI 1091
Score = 25.8 bits (54), Expect = 9.1
Identities = 21/76 (27%), Positives = 23/76 (30%), Gaps = 6/76 (7%)
Frame = +2
Query: 311 PXXPTSGGXMKXGPXTXAPPPXXXPLSPXXXAPPPXQXXQVLXPXVP---XFXXPXPXP- 478
P P S P P P P PP + P VP P P P
Sbjct: 1050 PPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGI-PPVPKPSVAAPPVPKPS 1108
Query: 479 --XXPLPLPXXAXPXP 520
P+P P A P P
Sbjct: 1109 VAVPPVPAPSGAPPVP 1124
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.1 bits (67), Expect = 0.24
Identities = 18/60 (30%), Positives = 21/60 (35%)
Frame = +2
Query: 347 GPXTXAPPPXXXPLSPXXXAPPPXQXXQVLXPXVPXFXXPXPXPXXPLPLPXXAXPXPPL 526
G A P P+ P APP P P P P P+P + P PPL
Sbjct: 1679 GGMAPAHPVSTPPVRPQSAAPPQMSAPT---PPPPPMSVPPPPSAPPMPAGPPSAPPPPL 1735
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 31.1 bits (67), Expect = 0.24
Identities = 20/61 (32%), Positives = 23/61 (37%), Gaps = 2/61 (3%)
Frame = +2
Query: 350 PXTXAPPPXXXPLSPXXXAPPPXQXXQVLXPXVPXFXXPXPXPXXPLP--LPXXAXPXPP 523
P APP L P APP + P P P P P+P LP A P P
Sbjct: 125 PSAPAPPTPQSELRPPTSAPPRPS----IPPPSPASAPPIPSKAPPIPSSLPPPAQPAAP 180
Query: 524 L 526
+
Sbjct: 181 V 181
Score = 30.3 bits (65), Expect = 0.42
Identities = 22/71 (30%), Positives = 27/71 (38%), Gaps = 1/71 (1%)
Frame = +2
Query: 311 PXXPTSGGXMKXGPXTXAPP-PXXXPLSPXXXAPPPXQXXQVLXPXVPXFXXPXPXPXXP 487
P PT ++ P T APP P P SP P P + P +P P P P
Sbjct: 128 PAPPTPQSELR--PPTSAPPRPSIPPPSPASAPPIPSK-----APPIPSSLPPPAQPAAP 180
Query: 488 LPLPXXAXPXP 520
+ P A P
Sbjct: 181 VKSPPSAPSLP 191
Score = 28.7 bits (61), Expect = 1.3
Identities = 11/21 (52%), Positives = 11/21 (52%)
Frame = +2
Query: 464 PXPXPXXPLPLPXXAXPXPPL 526
P P P P P P A P PPL
Sbjct: 3 PAPPPPPPAPAPAAAAPAPPL 23
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 27.1 bits (57), Expect = 4.0
Identities = 16/53 (30%), Positives = 16/53 (30%)
Frame = -1
Query: 522 GGXGXAXXGSGRGXXGXGXGXXNXGTXGXSTWXXWXGGGAXXXGDXGXXXGGG 364
G G G G G G G G G GGG G GGG
Sbjct: 212 GFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGG 264
>SPBC1289.16c ||SPBC8E4.06|copper amine oxidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 794
Score = 26.6 bits (56), Expect = 5.2
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = +1
Query: 100 HMPSTDEDTPRGLSGWV 150
+ P +DTP+GLS W+
Sbjct: 565 YAPQASDDTPKGLSKWI 581
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 25.8 bits (54), Expect = 9.1
Identities = 18/62 (29%), Positives = 20/62 (32%)
Frame = -1
Query: 513 GXAXXGSGRGXXGXGXGXXNXGTXGXSTWXXWXGGGAXXXGDXGXXXGGGAXVXGPXFMX 334
G GRG G G G G G + GGA G G G G G +
Sbjct: 17 GRGGFNGGRGGFGGGRGGARGGGRGGARGGRGGRGGA-RGGRGGSSGGRGGAKGGAKVII 75
Query: 333 PP 328
P
Sbjct: 76 EP 77
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,647,369
Number of Sequences: 5004
Number of extensions: 19357
Number of successful extensions: 105
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 52
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 92
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 495302128
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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