Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP06_F_J21
(881 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 107 2e-24
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 76 6e-15
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 73 7e-14
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 71 3e-13
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 67 4e-12
SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces pombe... 38 0.001
SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe... 38 0.002
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 28 2.0
SPBC32F12.02 |rec14||recombination protein Rec14|Schizosaccharom... 27 4.7
SPBC3E7.10 |fma1||methionine aminopeptidase Fma1 |Schizosaccharo... 26 8.2
>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 360
Score = 107 bits (258), Expect = 2e-24
Identities = 57/145 (39%), Positives = 80/145 (55%), Gaps = 1/145 (0%)
Frame = +3
Query: 99 AAVLHGANDVRIEKIPVPEINDD-EVLIKIDCVGICGSDVKLYSTGTCGADVIDKPIVIG 275
A VL D IE P + DD +V + I GICGSDV + G G ++ KP+++G
Sbjct: 7 AFVLRKKMDTAIEDRPGQTLTDDHQVKVAIKATGICGSDVHYWKEGGIGDFILKKPMILG 66
Query: 276 HXGAGTVVKVGDKVSSLRVGDRVAIEPTQPCRSCELCKRGKYNLCVEPRYCSSMGRSGKP 455
H AG VV+VG VSSL+ GD VA+EP CR C+ C+ G+YNLC + ++ G
Sbjct: 67 HESAGVVVEVGKGVSSLKPGDPVAVEPGCVCRLCDYCRSGRYNLCPHMEFAATPPYDG-T 125
Query: 456 MPLLPXXSPIFCHXLPDHLTMEEXA 530
+ + FC LP +++EE A
Sbjct: 126 LRTYYITTEDFCTKLPKQISVEEGA 150
>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
Adh1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 350
Score = 76.2 bits (179), Expect = 6e-15
Identities = 42/109 (38%), Positives = 62/109 (56%), Gaps = 5/109 (4%)
Frame = +3
Query: 99 AAVLH---GANDVRIEKIPVPEINDDEVLIKIDCVGICGSDVK-LYSTGTCGADVIDKPI 266
AAV H G +V+ E++PV E DEVL+ I G+C +D+ L A + P+
Sbjct: 9 AAVFHTHGGPENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQGDWPLPAKM---PL 65
Query: 267 VIGHXGAGTVVKVGDKVSSLRVGDRVAIE-PTQPCRSCELCKRGKYNLC 410
+ GH GAG VVKVG V+ L++GDRV ++ C +CE C + + +C
Sbjct: 66 IGGHEGAGVVVKVGAGVTRLKIGDRVGVKWMNSSCGNCEYCMKAEETIC 114
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 72.5 bits (170), Expect = 7e-14
Identities = 44/116 (37%), Positives = 60/116 (51%), Gaps = 2/116 (1%)
Frame = +3
Query: 99 AAVLHGAND-VRIEKIPVPEINDDEVLIKIDCVGICGSDVKLYSTGTCGADVIDK-PIVI 272
AAV GA + + IE I V EV +K+D +C +D S G D PIV+
Sbjct: 12 AAVAWGAKEPLSIEDIQVAPPKAHEVRVKVDWSAVCHTDAYTLS----GVDPEGAFPIVL 67
Query: 273 GHXGAGTVVKVGDKVSSLRVGDRVAIEPTQPCRSCELCKRGKYNLCVEPRYCSSMG 440
GH GAG V +G+ V ++R GD V + T C+ C+ C+ GK NLC + R G
Sbjct: 68 GHEGAGIVESIGEGVINVRPGDHVILLYTPECKECKFCRSGKTNLCSKIRETQGRG 123
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 70.5 bits (165), Expect = 3e-13
Identities = 39/106 (36%), Positives = 58/106 (54%), Gaps = 2/106 (1%)
Frame = +3
Query: 99 AAVLHGANDVRIEKIPVPEI-NDDEVLIKIDCVGIC-GSDVKLYSTGTCGADVIDKPIVI 272
A V G +V+I ++P P I + +V++K IC GSD ++S G I+K ++
Sbjct: 39 ACVWDGPLNVKIAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPG---IEKGAIL 95
Query: 273 GHXGAGTVVKVGDKVSSLRVGDRVAIEPTQPCRSCELCKRGKYNLC 410
GH G V + GD+V++L +GDRV I C C CKR +Y C
Sbjct: 96 GHESCGIVAEKGDEVNNLEIGDRVVIAFDLACGQCSFCKRHEYAAC 141
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 66.9 bits (156), Expect = 4e-12
Identities = 45/126 (35%), Positives = 61/126 (48%), Gaps = 1/126 (0%)
Frame = +3
Query: 66 KLTGCKMTENYAAVLHGANDVRIEKIPVPEINDDEVLIKIDCVGICGSDVKLYSTGTCGA 245
K+ CK A A + IE + V EV IKI G+C +D S G
Sbjct: 9 KIINCKA----AVAWQPAAPLSIENVQVFPPRVHEVRIKIVNSGVCHTDAYTLS----GK 60
Query: 246 DVIDK-PIVIGHXGAGTVVKVGDKVSSLRVGDRVAIEPTQPCRSCELCKRGKYNLCVEPR 422
D P+++GH GAG V VG +V++++VGD V T C++C+ CK GK NLC R
Sbjct: 61 DPEGLFPVILGHEGAGIVESVGPQVTTVQVGDPVIALYTPECKTCKFCKSGKTNLCGRIR 120
Query: 423 YCSSMG 440
G
Sbjct: 121 TTQGKG 126
>SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 325
Score = 38.3 bits (85), Expect = 0.001
Identities = 22/73 (30%), Positives = 38/73 (52%)
Frame = +3
Query: 114 GANDVRIEKIPVPEINDDEVLIKIDCVGICGSDVKLYSTGTCGADVIDKPIVIGHXGAGT 293
G + I+ +P+P+ + E+L+KI+ I SD+ + +TG G P ++G AGT
Sbjct: 19 GCLGIEIQSVPIPQPKNGELLVKIEAAAINPSDL-MNATG--GFPYTVYPRIVGRDYAGT 75
Query: 294 VVKVGDKVSSLRV 332
V+ + RV
Sbjct: 76 VISGASHLVGTRV 88
>SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 346
Score = 37.9 bits (84), Expect = 0.002
Identities = 24/80 (30%), Positives = 41/80 (51%), Gaps = 1/80 (1%)
Frame = +3
Query: 108 LHGANDVRIEKIPVPE-INDDEVLIKIDCVGICGSDVKLYSTGTCGADVIDKPIVIGHXG 284
+ G + ++ E+ VP+ +N EVL+K+ + D+ + + G + P+V G G
Sbjct: 11 ISGFDQLKPEEYEVPQKLNPGEVLVKLKAASLNYRDL-IITKGLYPLP-LQLPVVPGSDG 68
Query: 285 AGTVVKVGDKVSSLRVGDRV 344
AG + KVG+ V GD V
Sbjct: 69 AGIIEKVGEDVEGFEKGDSV 88
>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
Length = 582
Score = 27.9 bits (59), Expect = 2.0
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +3
Query: 231 GTCGADVIDKPIVIGHXGAGTVVKVGDKVSSLR 329
G+CG+ + K I GAG VKV D+ +R
Sbjct: 349 GSCGSVTVTKEDTIIMKGAGDHVKVNDRCEQIR 381
>SPBC32F12.02 |rec14||recombination protein
Rec14|Schizosaccharomyces pombe|chr 2|||Manual
Length = 302
Score = 26.6 bits (56), Expect = 4.7
Identities = 11/38 (28%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +3
Query: 306 GDKVSSLRVGDRVAI-EPTQPCRSCELCKRGKYNLCVE 416
G ++ V R+A+ + C+ EL +GK+ LC++
Sbjct: 115 GQTIAFTSVDGRIAVWDNPSDCKISELDTKGKFGLCID 152
>SPBC3E7.10 |fma1||methionine aminopeptidase Fma1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 379
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/44 (29%), Positives = 22/44 (50%), Gaps = 2/44 (4%)
Frame = +3
Query: 399 YNLCVEPRYC--SSMGRSGKPMPLLPXXSPIFCHXLPDHLTMEE 524
+N C+E R C S++ P + + I CH +PD +E+
Sbjct: 160 HNACIE-RDCFPSTLNYYAFPKSVCTSVNEIICHGIPDQRPLED 202
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,886,583
Number of Sequences: 5004
Number of extensions: 50709
Number of successful extensions: 110
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 108
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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