Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP06_F_E01
(880 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 36 0.006
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 34 0.031
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 32 0.12
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.22
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 30 0.38
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 1.2
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 27 2.7
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 8.1
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 36.3 bits (80), Expect = 0.006
Identities = 18/51 (35%), Positives = 19/51 (37%), Gaps = 3/51 (5%)
Frame = +1
Query: 721 PPPPXLLXXPXXXPXTXPXAPXXP---HXPXPXXPXXXXXRPPPXPPXHPP 864
PPPP + P P P P P P P P PP PP PP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 32.7 bits (71), Expect = 0.071
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = +1
Query: 496 PXPXFFFFXXXPPPPPPXXQKXXPPPXPP 582
P P PPPPPP PPP PP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 29.1 bits (62), Expect = 0.87
Identities = 13/46 (28%), Positives = 15/46 (32%)
Frame = +3
Query: 495 PPPQXFFFXXKXPPPPPXXTKXXXPPXPAXXKTXPXPPXGXFSPPP 632
PPP PPPPP PP P ++P P
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAP 797
Score = 28.7 bits (61), Expect = 1.2
Identities = 14/37 (37%), Positives = 14/37 (37%)
Frame = +1
Query: 472 APXFXGVXPXPXFFFFXXXPPPPPPXXQKXXPPPXPP 582
AP V P PPPPP PPP PP
Sbjct: 745 APAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 27.9 bits (59), Expect = 2.0
Identities = 14/36 (38%), Positives = 14/36 (38%)
Frame = +3
Query: 525 KXPPPPPXXTKXXXPPXPAXXKTXPXPPXGXFSPPP 632
K PPPPP P PA P P PPP
Sbjct: 730 KSPPPPPPAV-IVPTPAPAPIPVPPPAPIMGGPPPP 764
Score = 27.5 bits (58), Expect = 2.7
Identities = 11/27 (40%), Positives = 11/27 (40%)
Frame = +1
Query: 529 PPPPPPXXQKXXPPPXPPXKKXXXXPP 609
P PPP PPP PP PP
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPP 776
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 33.9 bits (74), Expect = 0.031
Identities = 18/39 (46%), Positives = 18/39 (46%)
Frame = -2
Query: 645 GGGGXGGGKXXRXGGXVXFFXGRGGGGXXFLXXGGGGGG 529
G GG GGG GG F G GG G GGG GG
Sbjct: 222 GHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGG 260
Score = 30.7 bits (66), Expect = 0.29
Identities = 23/66 (34%), Positives = 24/66 (36%)
Frame = -2
Query: 657 PRXXGGGGXGGGKXXRXGGXVXFFXGRGGGGXXFLXXGGGGGGXXXKKKKXGXGXXXXKX 478
P GG G GG+ GG F G G GG G GGG G G
Sbjct: 204 PGGFGGFGGFGGEGHHHGGHGGF--GGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGF 261
Query: 477 GGPPXG 460
GG P G
Sbjct: 262 GGGPGG 267
Score = 29.9 bits (64), Expect = 0.50
Identities = 24/74 (32%), Positives = 25/74 (33%), Gaps = 1/74 (1%)
Frame = -2
Query: 645 GGGGXGGGKXXRXGGXVXFFXGRGGGGXXFLXXGG-GGGGXXXKKKKXGXGXXXXKXGGP 469
GG G G G G F G GG G GG GGG + G G GG
Sbjct: 191 GGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGG 250
Query: 468 PXGXKKXXXPXGGG 427
G GGG
Sbjct: 251 LGGFGGGPGGFGGG 264
Score = 25.8 bits (54), Expect = 8.1
Identities = 20/55 (36%), Positives = 21/55 (38%), Gaps = 2/55 (3%)
Frame = -1
Query: 628 GGEKXPXGGXGXVFXXAGX--GGXXFFVXXGGGGGXFXXKKKXWGGGXXXXXXGP 470
GGE GG G G GG F GGG G F +GGG GP
Sbjct: 214 GGEGHHHGGHGGFGGGPGGFEGGPGGF---GGGPGGFGGGLGGFGGGPGGFGGGP 265
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 31.9 bits (69), Expect = 0.12
Identities = 25/72 (34%), Positives = 27/72 (37%), Gaps = 3/72 (4%)
Frame = -2
Query: 645 GGGGXGGGKXXRXGGXVXFFXGRG---GGGXXFLXXGGGGGGXXXKKKKXGXGXXXXKXG 475
G GG GG+ GG F GRG GGG G GG G + G G G
Sbjct: 10 GRGGSRGGRGGFNGGRGGFGGGRGGARGGGRGGARGGRGGRGG----ARGGRGGSSGGRG 65
Query: 474 GPPXGXKKXXXP 439
G G K P
Sbjct: 66 GAKGGAKVIIEP 77
Score = 31.1 bits (67), Expect = 0.22
Identities = 22/60 (36%), Positives = 25/60 (41%), Gaps = 1/60 (1%)
Frame = -2
Query: 636 GXGGGKXXRXGGXVXFFXGRGG-GGXXFLXXGGGGGGXXXKKKKXGXGXXXXKXGGPPXG 460
G GG+ GG F GRGG GG GGG GG + + G G GG G
Sbjct: 6 GSRGGRGGSRGGRGGFNGGRGGFGGGRGGARGGGRGG--ARGGRGGRGGARGGRGGSSGG 63
Score = 31.1 bits (67), Expect = 0.22
Identities = 19/48 (39%), Positives = 20/48 (41%)
Frame = -3
Query: 869 GXGGWXGGXGGGR*XXXXGXXGXGXCGXXGAXGXVXGXXXGXXRRXGG 726
G GG+ GG GG R G G G G GA G G G GG
Sbjct: 24 GRGGFGGGRGGARGGGRGGARG-GRGGRGGARGGRGGSSGGRGGAKGG 70
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 31.1 bits (67), Expect = 0.22
Identities = 25/116 (21%), Positives = 27/116 (23%)
Frame = +1
Query: 529 PPPPPPXXQKXXPPPXPPXKKXXXXPPXXXXXXXXXXXXXXXXXXXXXXXXXTKHPXXXX 708
PPPPPP P PP + PP P
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPG 397
Query: 709 XXGXPPPPXLLXXPXXXPXTXPXAPXXPHXPXPXXPXXXXXRPPPXPPXHPPXPXL 876
PP + P P P P P PP P P P L
Sbjct: 398 RSAPALPP----LGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPL 449
Score = 30.3 bits (65), Expect = 0.38
Identities = 28/118 (23%), Positives = 29/118 (24%), Gaps = 4/118 (3%)
Frame = +1
Query: 529 PPPPPP--XXQKXXPPPXPPXKKXXXXPPXXXXXXXXXXXXXXXXXXXXXXXXXTKHPXX 702
PPPPPP PP + PP P
Sbjct: 361 PPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTP 420
Query: 703 XXXXGXPPPPXLLXXPXXXPXTXPXAPXXPHXPXPXXPXXXXXRP--PPXPPXHPPXP 870
PP P P P AP P P P P PP PP P P
Sbjct: 421 PSLPPSAPPSLPPSAPPSLPMGAPAAPPLP-PSAPIAPPLPAGMPAAPPLPPAAPAPP 477
Score = 29.1 bits (62), Expect = 0.87
Identities = 27/115 (23%), Positives = 28/115 (24%), Gaps = 3/115 (2%)
Frame = +1
Query: 529 PPPPPPXXQKXXPPPXPPXKKXXXXPPXXXXXXXXXXXXXXXXXXXXXXXXXTKHPXXXX 708
PPPPPP + PP PP P
Sbjct: 312 PPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPP-PRSAP 370
Query: 709 XXGXPPPPXLLXXPXXXPXTXPXAPXXPHXP--XPXXPXXXXXRPP-PXPPXHPP 864
G PPP P P A P P PP P PP PP
Sbjct: 371 STGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPP 425
Score = 29.1 bits (62), Expect = 0.87
Identities = 14/34 (41%), Positives = 16/34 (47%)
Frame = +3
Query: 531 PPPPPXXTKXXXPPXPAXXKTXPXPPXGXFSPPP 632
PPPPP PP + P PP G +PPP
Sbjct: 337 PPPPP-------PPRSNAAGSIPLPPQGRSAPPP 363
Score = 28.3 bits (60), Expect = 1.5
Identities = 11/32 (34%), Positives = 14/32 (43%)
Frame = +3
Query: 531 PPPPPXXTKXXXPPXPAXXKTXPXPPXGXFSP 626
PPPPP P P ++ P PP +P
Sbjct: 339 PPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAP 370
Score = 27.1 bits (57), Expect = 3.5
Identities = 15/45 (33%), Positives = 17/45 (37%)
Frame = +3
Query: 498 PPQXFFFXXKXPPPPPXXTKXXXPPXPAXXKTXPXPPXGXFSPPP 632
PP PP PP + PP PA P P +PPP
Sbjct: 436 PPSLPMGAPAAPPLPP--SAPIAPPLPAGMPAAPPLPPAAPAPPP 478
Score = 26.6 bits (56), Expect = 4.7
Identities = 19/54 (35%), Positives = 19/54 (35%), Gaps = 4/54 (7%)
Frame = +1
Query: 721 PP--PPXLLXXPXXXPXTXPXAPXXPHXP--XPXXPXXXXXRPPPXPPXHPPXP 870
PP PP L P P AP P P P P P P PP P P
Sbjct: 432 PPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAP-PPAPAPAP 484
Score = 26.2 bits (55), Expect = 6.1
Identities = 13/56 (23%), Positives = 18/56 (32%)
Frame = +1
Query: 685 TKHPXXXXXXGXPPPPXLLXXPXXXPXTXPXAPXXPHXPXPXXPXXXXXRPPPXPP 852
++ P P PP + + P +P P P P PP PP
Sbjct: 239 SRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPP 294
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 30.3 bits (65), Expect = 0.38
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = +1
Query: 529 PPPPPPXXQKXXPPPXPP 582
PPPPPP + PP PP
Sbjct: 11 PPPPPPGFEPPSQPPPPP 28
Score = 27.1 bits (57), Expect = 3.5
Identities = 11/20 (55%), Positives = 11/20 (55%), Gaps = 2/20 (10%)
Frame = +1
Query: 529 PPPPPP--XXQKXXPPPXPP 582
PPPPPP PPP PP
Sbjct: 10 PPPPPPPGFEPPSQPPPPPP 29
Score = 25.8 bits (54), Expect = 8.1
Identities = 9/17 (52%), Positives = 9/17 (52%)
Frame = +1
Query: 532 PPPPPXXQKXXPPPXPP 582
PPPPP PP PP
Sbjct: 9 PPPPPPPPGFEPPSQPP 25
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.7 bits (61), Expect = 1.2
Identities = 14/35 (40%), Positives = 15/35 (42%), Gaps = 2/35 (5%)
Frame = +3
Query: 531 PPPPPXXTKXXX--PPXPAXXKTXPXPPXGXFSPP 629
PPPPP PP PA + P PP S P
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741
Score = 25.8 bits (54), Expect = 8.1
Identities = 9/18 (50%), Positives = 9/18 (50%)
Frame = +1
Query: 529 PPPPPPXXQKXXPPPXPP 582
P PPPP PP PP
Sbjct: 1705 PTPPPPPMSVPPPPSAPP 1722
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 27.5 bits (58), Expect = 2.7
Identities = 19/52 (36%), Positives = 21/52 (40%)
Frame = -3
Query: 875 RXGXGGWXGGXGGGR*XXXXGXXGXGXCGXXGAXGXVXGXXXGXXRRXGGGG 720
R G GG+ GG GG R G G G G+ G G G R GG
Sbjct: 137 RGGRGGFRGGRGGSR--GGFGGNSRGGFG-GGSRGGFGGGSRGGSRGGFRGG 185
Score = 27.1 bits (57), Expect = 3.5
Identities = 19/50 (38%), Positives = 21/50 (42%), Gaps = 1/50 (2%)
Frame = -2
Query: 675 KKKKAXPRXXGG-GGXGGGKXXRXGGXVXFFXGRGGGGXXFLXXGGGGGG 529
K + P GG GG GG+ GG F G GG GG GGG
Sbjct: 128 KGARNGPAGRGGRGGFRGGRGGSRGG----FGGNSRGGFGGGSRGGFGGG 173
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 25.8 bits (54), Expect = 8.1
Identities = 9/18 (50%), Positives = 9/18 (50%)
Frame = +1
Query: 529 PPPPPPXXQKXXPPPXPP 582
PPPPPP P PP
Sbjct: 5 PPPPPPAPAPAAAAPAPP 22
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,741,897
Number of Sequences: 5004
Number of extensions: 24509
Number of successful extensions: 249
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 54
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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