Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP06_F_D01
(920 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 37 0.005
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 35 0.014
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 33 0.075
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 31 0.23
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 31 0.30
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 29 0.70
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 29 1.2
SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit Vps23|Schizosac... 28 2.1
SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ... 27 2.8
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 4.9
SPAC1F12.06c |||endonuclease |Schizosaccharomyces pombe|chr 1|||... 27 4.9
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 26 8.6
SPCC1739.06c |||uroporphyrin methyltransferase |Schizosaccharomy... 26 8.6
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 36.7 bits (81), Expect = 0.005
Identities = 29/94 (30%), Positives = 29/94 (30%), Gaps = 3/94 (3%)
Frame = +2
Query: 638 PPXXQTXXXGXSAP--PPXPXLXRXQTPXFPLPXXXXPP-PPXXPXXXXXXXXXXXXXTX 808
PP G SAP PP R TP P P P PP P
Sbjct: 388 PPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAA- 446
Query: 809 PPPXPFFXSXAPRGXGGXPXXXXPPXTPXPPPPA 910
PP P AP G P P PPPA
Sbjct: 447 -PPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPA 479
Score = 32.3 bits (70), Expect = 0.100
Identities = 34/143 (23%), Positives = 39/143 (27%), Gaps = 1/143 (0%)
Frame = +2
Query: 485 PKXKNKKKXQXXKTXXNPXXXPPPXPXXXXXXPXXXSXXXXXXXXXGXX-PPPPXXQTXX 661
P +N+ K N PPP P P + G PPPP ++
Sbjct: 317 PSRRNRGKPPIGNGSSNSSLPPPPPP------PRSNAAGSIPLPPQGRSAPPPPPPRSAP 370
Query: 662 XGXSAPPPXPXLXRXQTPXFPLPXXXXPPPPXXPXXXXXXXXXXXXXTXPPPXPFFXSXA 841
PPP P P P P P T PP P S
Sbjct: 371 STGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALP------PLGNASRTSTPPVPTPPSLP 424
Query: 842 PRGXGGXPXXXXPPXTPXPPPPA 910
P P PP P P A
Sbjct: 425 PSAPPSLP-PSAPPSLPMGAPAA 446
Score = 32.3 bits (70), Expect = 0.100
Identities = 17/65 (26%), Positives = 20/65 (30%)
Frame = +1
Query: 550 PXXPSXXXXXPPAXVXXXPXPXXXGXXPPPPXXPNXXXRXXCPPPXPXPXPXXNXXXSPP 729
P PS PP+ P G PP P+ P P P +PP
Sbjct: 418 PTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPP 477
Query: 730 PXXXP 744
P P
Sbjct: 478 PAPAP 482
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 35.1 bits (77), Expect = 0.014
Identities = 17/50 (34%), Positives = 19/50 (38%), Gaps = 1/50 (2%)
Frame = +1
Query: 550 PXXPSXXXXXP-PAXVXXXPXPXXXGXXPPPPXXPNXXXRXXCPPPXPXP 696
P P+ P PA + P G PPPP P PPP P P
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 29.9 bits (64), Expect = 0.53
Identities = 13/37 (35%), Positives = 13/37 (35%)
Frame = +1
Query: 634 PPPXXPNXXXRXXCPPPXPXPXPXXNXXXSPPPXXXP 744
PPP P P P P P P PPP P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPP 768
Score = 29.9 bits (64), Expect = 0.53
Identities = 17/69 (24%), Positives = 18/69 (26%)
Frame = +2
Query: 548 PPPXPXXXXXXPXXXSXXXXXXXXXGXXPPPPXXQTXXXGXSAPPPXPXLXRXQTPXFPL 727
PPP P G PPPP G PPP P +
Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYA 794
Query: 728 PXXXXPPPP 754
P P P
Sbjct: 795 PAPQAEPEP 803
Score = 29.1 bits (62), Expect = 0.93
Identities = 18/56 (32%), Positives = 18/56 (32%)
Frame = +2
Query: 743 PPPPXXPXXXXXXXXXXXXXTXPPPXPFFXSXAPRGXGGXPXXXXPPXTPXPPPPA 910
PPPP P PPP P P PP P PPPPA
Sbjct: 732 PPPP--PPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP-PPPPA 784
Score = 28.3 bits (60), Expect = 1.6
Identities = 12/38 (31%), Positives = 13/38 (34%)
Frame = +3
Query: 804 PXPPPXLFFXPXXPXGXAGXXPXXPXLXXPXPPPXRXG 917
P PPP P P P + P PPP G
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPG 769
Score = 27.5 bits (58), Expect = 2.8
Identities = 12/34 (35%), Positives = 12/34 (35%)
Frame = +1
Query: 631 PPPPXXPNXXXRXXCPPPXPXPXPXXNXXXSPPP 732
PPPP P P P P P PPP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPP 766
Score = 25.8 bits (54), Expect = 8.6
Identities = 16/51 (31%), Positives = 17/51 (33%)
Frame = +2
Query: 671 SAPPPXPXLXRXQTPXFPLPXXXXPPPPXXPXXXXXXXXXXXXXTXPPPXP 823
S PPP P + TP P P PP P PPP P
Sbjct: 731 SPPPPPPAVI-VPTPA-PAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP 779
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 32.7 bits (71), Expect = 0.075
Identities = 24/98 (24%), Positives = 29/98 (29%), Gaps = 7/98 (7%)
Frame = +2
Query: 638 PPXXQTXXXGXSAPPPXPXLXRXQTPXFPLPXXXXP-------PPPXXPXXXXXXXXXXX 796
PP ++ SAPPP P + P P P P PP P
Sbjct: 1050 PPVPKSSSGAPSAPPPVPA-PSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPS 1108
Query: 797 XXTXPPPXPFFXSXAPRGXGGXPXXXXPPXTPXPPPPA 910
P P P P+ P P P P P+
Sbjct: 1109 VAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPS 1146
Score = 28.7 bits (61), Expect = 1.2
Identities = 21/79 (26%), Positives = 23/79 (29%)
Frame = +2
Query: 674 APPPXPXLXRXQTPXFPLPXXXXPPPPXXPXXXXXXXXXXXXXTXPPPXPFFXSXAPRGX 853
A PP P P P P PP P P P P P P+
Sbjct: 1148 AAPPVPA--PSGAPPVPKPSVAAPPVPA-PSSGIPPVPKPAAGVPPVPPPSEAPPVPKPS 1204
Query: 854 GGXPXXXXPPXTPXPPPPA 910
G P P P P P+
Sbjct: 1205 VGVPPVPPPSTAPPVPTPS 1223
Score = 27.1 bits (57), Expect = 3.7
Identities = 20/94 (21%), Positives = 24/94 (25%), Gaps = 1/94 (1%)
Frame = +2
Query: 632 PPPPXXQTXXXGXSAPPPXPXLXRXQTPXFPLPXXXXPPPPXXPXXXXXXXXXXXXXTXP 811
PP P AP P + + P+P PP P
Sbjct: 1102 PPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPV 1161
Query: 812 PPXPFFXSXAPRGXGGXPXXXXP-PXTPXPPPPA 910
P P G P P P PPP+
Sbjct: 1162 PKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPS 1195
Score = 26.6 bits (56), Expect = 4.9
Identities = 13/31 (41%), Positives = 13/31 (41%), Gaps = 3/31 (9%)
Frame = +1
Query: 664 RXXCPPPXPXPXPXXNXXXSP---PPXXXPP 747
R PP P P P N SP PP PP
Sbjct: 962 RPAAPPSIPPPLPVSNILSSPTSEPPKDHPP 992
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 31.1 bits (67), Expect = 0.23
Identities = 22/60 (36%), Positives = 23/60 (38%)
Frame = -2
Query: 901 GGXGXXRXGXXGXXPAXPXGXXGXKKRXGGGXGXRXXRGGRV*GGXGXXGRXGGGXXGEG 722
GG G G G P G G GGG G GG + G G G GGG G G
Sbjct: 214 GGEGHHHGGHGGFG-GGPGGFEGGPGGFGGGPG---GFGGGLGGFGGGPGGFGGGPGGHG 269
Score = 25.8 bits (54), Expect = 8.6
Identities = 18/53 (33%), Positives = 18/53 (33%)
Frame = -2
Query: 901 GGXGXXRXGXXGXXPAXPXGXXGXKKRXGGGXGXRXXRGGRV*GGXGXXGRXG 743
GG G G G P G G GGG G G GG G G G
Sbjct: 221 GGHGGF-GGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGGHGGPG 272
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 30.7 bits (66), Expect = 0.30
Identities = 23/60 (38%), Positives = 24/60 (40%), Gaps = 2/60 (3%)
Frame = -2
Query: 901 GGXGXXRXGXXGXXPAXPXGXXGXK--KRXGGGXGXRXXRGGRV*GGXGXXGRXGGGXXG 728
GG G R G G G G + R GG G R RGGR G G G GG G
Sbjct: 9 GGRGGSRGGRGGFN-GGRGGFGGGRGGARGGGRGGARGGRGGRG-GARGGRGGSSGGRGG 66
Score = 29.5 bits (63), Expect = 0.70
Identities = 22/60 (36%), Positives = 22/60 (36%)
Frame = -2
Query: 901 GGXGXXRXGXXGXXPAXPXGXXGXKKRXGGGXGXRXXRGGRV*GGXGXXGRXGGGXXGEG 722
GG R G G G G R GG G R RGGR G G GG G G
Sbjct: 12 GGSRGGRGGFNGGRGGFGGGRGGA--RGGGRGGARGGRGGR----GGARGGRGGSSGGRG 65
Score = 27.5 bits (58), Expect = 2.8
Identities = 18/52 (34%), Positives = 19/52 (36%)
Frame = -2
Query: 877 GXXGXXPAXPXGXXGXKKRXGGGXGXRXXRGGRV*GGXGXXGRXGGGXXGEG 722
G G G G + GGG G RGG G G G GG G G
Sbjct: 9 GGRGGSRGGRGGFNGGRGGFGGGRGGA--RGGGRGGARGGRGGRGGARGGRG 58
Score = 26.2 bits (55), Expect = 6.5
Identities = 14/41 (34%), Positives = 15/41 (36%)
Frame = -2
Query: 904 GGGXGXXRXGXXGXXPAXPXGXXGXKKRXGGGXGXRXXRGG 782
GGG G R G G G G + GG G R G
Sbjct: 29 GGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGGRGGAKG 69
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 29.5 bits (63), Expect = 0.70
Identities = 20/80 (25%), Positives = 23/80 (28%), Gaps = 1/80 (1%)
Frame = +2
Query: 671 SAPPPXPXLXRXQTPXFPLPXXXXPPPPXXPXXXXXXXXXXXXXTXPPPX-PFFXSXAPR 847
SAP P + P P PPP + PPP P +P
Sbjct: 126 SAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPP 185
Query: 848 GXGGXPXXXXPPXTPXPPPP 907
P P PPPP
Sbjct: 186 SAPSLPSAVPPMPPKVPPPP 205
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 28.7 bits (61), Expect = 1.2
Identities = 17/46 (36%), Positives = 17/46 (36%)
Frame = -2
Query: 850 PXGXXGXKKRXGGGXGXRXXRGGRV*GGXGXXGRXGGGXXGEGKXR 713
P G G GG G R GG GG G R G G G R
Sbjct: 134 PAGRGGRGGFRGGRGGSRGGFGGNSRGGFGGGSRGGFGGGSRGGSR 179
Score = 28.3 bits (60), Expect = 1.6
Identities = 17/46 (36%), Positives = 17/46 (36%)
Frame = -2
Query: 859 PAXPXGXXGXKKRXGGGXGXRXXRGGRV*GGXGXXGRXGGGXXGEG 722
P P G GG G R RGG GG G R G G G
Sbjct: 124 PKKPKGARNGPAGRGGRGGFRGGRGGSR-GGFGGNSRGGFGGGSRG 168
>SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit
Vps23|Schizosaccharomyces pombe|chr 1|||Manual
Length = 487
Score = 27.9 bits (59), Expect = 2.1
Identities = 16/49 (32%), Positives = 23/49 (46%)
Frame = +3
Query: 171 VHAFVKRDAPKEDNSLNTLAESAKKTIEELREKVESALAPETVKKNFGT 317
V A +K+D +E S L TIE+L+ K E+ P K F +
Sbjct: 128 VQALIKQDFEREHTSPPELPTKLVNTIEKLKVKEENEAPPVIPAKPFSS 176
>SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2344
Score = 27.5 bits (58), Expect = 2.8
Identities = 15/57 (26%), Positives = 29/57 (50%)
Frame = -2
Query: 289 GAKADSTFSLNSSIVFFALSASVFRLLSSLGASRLTNACTLARQIANKIISSLFILD 119
G A + F ++ SIV+ A+ ++V + + A ++ NK+ S L++LD
Sbjct: 410 GYMAITKFEVSQSIVYSAIVSAVAEFIRQILAEDQLLLNNFFEELKNKLESDLYLLD 466
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.6 bits (56), Expect = 4.9
Identities = 15/51 (29%), Positives = 16/51 (31%)
Frame = +1
Query: 580 PPAXVXXXPXPXXXGXXPPPPXXPNXXXRXXCPPPXPXPXPXXNXXXSPPP 732
PP P P PPP P PPP P P + P P
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPP---PLPASSAPSVPNP 1746
Score = 26.6 bits (56), Expect = 4.9
Identities = 13/34 (38%), Positives = 13/34 (38%)
Frame = +2
Query: 809 PPPXPFFXSXAPRGXGGXPXXXXPPXTPXPPPPA 910
PPP P P P PP P PP PA
Sbjct: 1707 PPPPPMSVPPPP---SAPPMPAGPPSAPPPPLPA 1737
>SPAC1F12.06c |||endonuclease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 252
Score = 26.6 bits (56), Expect = 4.9
Identities = 16/75 (21%), Positives = 36/75 (48%)
Frame = -1
Query: 455 YSIMSRTLLHSIYLGLXT*ILKNYFSGFRCFRGLQIFIKFVEAVYHRAKVFLNSLRGQGG 276
Y + R +++ YL + + ++Y GF FR ++ ++ + + H+ ++ + + G G
Sbjct: 60 YDLEQRMIIYKDYLCIEK-LEEDYVPGFLSFREIKWYLPLLNHIPHQFRIDIILVDGNGV 118
Query: 275 FNFFPQFLNCFLRTL 231
+ L C L L
Sbjct: 119 LHPVGFGLACHLGVL 133
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 25.8 bits (54), Expect = 8.6
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = +1
Query: 622 GXXPPPPXXPNXXXRXXCPPPXP 690
G PPPP P PPP P
Sbjct: 7 GNPPPPPPPPGFEPPSQPPPPPP 29
Score = 25.8 bits (54), Expect = 8.6
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +1
Query: 631 PPPPXXPNXXXRXXCPPPXPXP 696
PPPP P PPP P P
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPP 30
>SPCC1739.06c |||uroporphyrin methyltransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 496
Score = 25.8 bits (54), Expect = 8.6
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = -2
Query: 259 NSSIVFFALSASVFRLLSSLGASRLTNACTLARQIANKIISSL 131
N S+ F L A+ + S +N C LA+++ ++SSL
Sbjct: 121 NPSLCSFTLPATWSEPPLQISLSTSSNGCRLAQRLLRHVVSSL 163
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,690,369
Number of Sequences: 5004
Number of extensions: 53297
Number of successful extensions: 274
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 215
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 468512460
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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