Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP06_F_C21
(1028 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 37 0.005
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 36 0.007
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 32 0.11
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 32 0.15
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 30 0.46
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 29 1.1
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.9
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 27 3.2
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 27 3.2
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 26 7.5
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 7.5
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 7.5
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 36.7 bits (81), Expect = 0.005
Identities = 14/28 (50%), Positives = 14/28 (50%)
Frame = +2
Query: 686 PPPPPPPXPXXAAGXXPPPPGXXGPPXP 769
PPPPPPP A PP G PP P
Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPP 364
Score = 29.9 bits (64), Expect = 0.61
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = +2
Query: 689 PPPPPPXPXXAAGXXPPP 742
PPPPPP + G PPP
Sbjct: 361 PPPPPPRSAPSTGRQPPP 378
Score = 25.8 bits (54), Expect = 9.9
Identities = 20/72 (27%), Positives = 22/72 (30%), Gaps = 9/72 (12%)
Frame = +2
Query: 692 PPPPPXPXXAAGXXPPPPGXXGPPXPXXXXXXXXXXXXXXXXPXRPXXPR---------X 844
P PPP P + G PP PP P P P R
Sbjct: 251 PAPPPIPPPSNGTVSSPPN--SPPRPIAPVSMNPAINSTSKPPLPPPSSRVSAAALAANK 308
Query: 845 RRXGXPPPAXRR 880
+R PPP RR
Sbjct: 309 KRPPPPPPPSRR 320
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 36.3 bits (80), Expect = 0.007
Identities = 13/20 (65%), Positives = 13/20 (65%)
Frame = +2
Query: 686 PPPPPPPXPXXAAGXXPPPP 745
PPPPPPP AG PPPP
Sbjct: 761 PPPPPPPPGVAGAGPPPPPP 780
Score = 35.5 bits (78), Expect = 0.012
Identities = 15/26 (57%), Positives = 15/26 (57%)
Frame = +2
Query: 692 PPPPPXPXXAAGXXPPPPGXXGPPXP 769
PPPPP P AG PPPP PP P
Sbjct: 761 PPPPPPPPGVAGAGPPPP----PPPP 782
Score = 33.1 bits (72), Expect = 0.065
Identities = 12/20 (60%), Positives = 12/20 (60%)
Frame = +2
Query: 686 PPPPPPPXPXXAAGXXPPPP 745
PPPPPPP A PPPP
Sbjct: 762 PPPPPPPGVAGAGPPPPPPP 781
Score = 30.7 bits (66), Expect = 0.35
Identities = 15/32 (46%), Positives = 15/32 (46%), Gaps = 4/32 (12%)
Frame = +2
Query: 686 PPPPPP----PXPXXAAGXXPPPPGXXGPPXP 769
PPPPPP P P A PPP G P P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPP 763
Score = 29.9 bits (64), Expect = 0.61
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +2
Query: 686 PPPPPPPXPXXAAG 727
PPPPPPP P +AG
Sbjct: 775 PPPPPPPPPAVSAG 788
Score = 29.5 bits (63), Expect = 0.80
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +2
Query: 686 PPPPPPPXPXXAAGXXPPPPGXXGPPXP 769
PPPPPPP A G P P P
Sbjct: 776 PPPPPPPPAVSAGGSRYYAPAPQAEPEP 803
Score = 28.7 bits (61), Expect = 1.4
Identities = 14/33 (42%), Positives = 14/33 (42%), Gaps = 5/33 (15%)
Frame = +2
Query: 686 PPPPPPPXPXXAAGXXPPPP-----GXXGPPXP 769
P P P P P G PPPP GPP P
Sbjct: 746 PAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPP 778
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 32.3 bits (70), Expect = 0.11
Identities = 12/23 (52%), Positives = 12/23 (52%)
Frame = +2
Query: 689 PPPPPPXPXXAAGXXPPPPGXXG 757
PPPPPP P PPPP G
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPPG 31
Score = 31.5 bits (68), Expect = 0.20
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = +2
Query: 686 PPPPPPPXPXXAAGXXPPPP 745
PPPPPPP PPPP
Sbjct: 9 PPPPPPPPGFEPPSQPPPPP 28
Score = 30.7 bits (66), Expect = 0.35
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = +2
Query: 686 PPPPPPPXPXXAAGXXPPPP 745
PPPPPPP + PPPP
Sbjct: 10 PPPPPPPGFEPPSQPPPPPP 29
Score = 30.7 bits (66), Expect = 0.35
Identities = 11/21 (52%), Positives = 12/21 (57%)
Frame = +2
Query: 686 PPPPPPPXPXXAAGXXPPPPG 748
PPPPPP + PPPPG
Sbjct: 11 PPPPPPGFEPPSQPPPPPPPG 31
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 31.9 bits (69), Expect = 0.15
Identities = 12/28 (42%), Positives = 13/28 (46%)
Frame = +2
Query: 686 PPPPPPPXPXXAAGXXPPPPGXXGPPXP 769
PP PP P + G P PP PP P
Sbjct: 1193 PPSEAPPVPKPSVGVPPVPPPSTAPPVP 1220
Score = 30.7 bits (66), Expect = 0.35
Identities = 15/32 (46%), Positives = 15/32 (46%), Gaps = 4/32 (12%)
Frame = +2
Query: 686 PPPPP----PPXPXXAAGXXPPPPGXXGPPXP 769
P P P PP P AAG P PP PP P
Sbjct: 1170 PVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVP 1201
Score = 29.1 bits (62), Expect = 1.1
Identities = 13/29 (44%), Positives = 14/29 (48%), Gaps = 1/29 (3%)
Frame = +2
Query: 686 PPPPPPPXPXXAAGXXP-PPPGXXGPPXP 769
PP PP P +AG P P P PP P
Sbjct: 1212 PPSTAPPVPTPSAGLPPVPVPTAKAPPVP 1240
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 30.3 bits (65), Expect = 0.46
Identities = 11/18 (61%), Positives = 11/18 (61%)
Frame = +2
Query: 689 PPPPPPXPXXAAGXXPPP 742
PPPPPP P AA PP
Sbjct: 5 PPPPPPAPAPAAAAPAPP 22
Score = 27.5 bits (58), Expect = 3.2
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +2
Query: 686 PPPPPPPXPXXAAGXXPPPP 745
P PPPPP A P PP
Sbjct: 3 PAPPPPPPAPAPAAAAPAPP 22
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 29.1 bits (62), Expect = 1.1
Identities = 20/62 (32%), Positives = 20/62 (32%), Gaps = 1/62 (1%)
Frame = -3
Query: 867 GGGXPXRRXRGXXGRXGXXXXXXXXXXXXAXXXGXGG-PXXPGGGGXXPAAXXGXGGGGG 691
G G P G G G G GG PGG G P G GG G
Sbjct: 196 GSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFG 255
Query: 690 GG 685
GG
Sbjct: 256 GG 257
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.3 bits (60), Expect = 1.9
Identities = 12/29 (41%), Positives = 12/29 (41%), Gaps = 1/29 (3%)
Frame = +2
Query: 686 PPPPPPPXPXXAAGXXPPP-PGXXGPPXP 769
PPP PP P PPP P P P
Sbjct: 1716 PPPSAPPMPAGPPSAPPPPLPASSAPSVP 1744
Score = 27.5 bits (58), Expect = 3.2
Identities = 13/32 (40%), Positives = 13/32 (40%), Gaps = 4/32 (12%)
Frame = +2
Query: 686 PPPPPPPXPXXAAGXXPP----PPGXXGPPXP 769
P PPPPP PP PP PP P
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
Score = 27.5 bits (58), Expect = 3.2
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +3
Query: 657 PRRPPXXAXXPRRPPPPXP 713
P PP A P PPPP P
Sbjct: 1718 PSAPPMPAGPPSAPPPPLP 1736
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 27.5 bits (58), Expect = 3.2
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +2
Query: 689 PPPPPPXPXXAAGXXPPPP 745
P PP P P A PPPP
Sbjct: 729 PAPPTPAPTPAVKHHPPPP 747
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 27.5 bits (58), Expect = 3.2
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = +2
Query: 686 PPPPPPPXPXXAA 724
PPPPPPP P +A
Sbjct: 945 PPPPPPPPPLVSA 957
Score = 27.1 bits (57), Expect = 4.3
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +2
Query: 686 PPPPPPPXPXXAAG 727
PPPPPPP AAG
Sbjct: 946 PPPPPPPPLVSAAG 959
Score = 26.6 bits (56), Expect = 5.7
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = +2
Query: 467 PXXPPPXPGPPPXXRAA 517
P PPP P PPP AA
Sbjct: 942 PAFPPPPPPPPPLVSAA 958
Score = 26.6 bits (56), Expect = 5.7
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +2
Query: 686 PPPPPPPXPXXAAGXXPPP 742
PPPPPPP A G P
Sbjct: 947 PPPPPPPLVSAAGGKFVSP 965
Score = 23.4 bits (48), Expect(2) = 8.3
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = +2
Query: 689 PPPPPPXP 712
PPPPPP P
Sbjct: 907 PPPPPPLP 914
Score = 20.6 bits (41), Expect(2) = 8.3
Identities = 6/7 (85%), Positives = 6/7 (85%)
Frame = +2
Query: 686 PPPPPPP 706
P PPPPP
Sbjct: 905 PTPPPPP 911
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 26.2 bits (55), Expect = 7.5
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +2
Query: 686 PPPPPPPXP 712
PPPPPPP P
Sbjct: 306 PPPPPPPPP 314
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.2 bits (55), Expect = 7.5
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +2
Query: 686 PPPPPPPXP 712
PPPPPPP P
Sbjct: 189 PPPPPPPPP 197
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 26.2 bits (55), Expect = 7.5
Identities = 11/25 (44%), Positives = 11/25 (44%)
Frame = +2
Query: 689 PPPPPPXPXXAAGXXPPPPGXXGPP 763
PPPPPP AG P PP
Sbjct: 1882 PPPPPPMALPKAGPPSAAPTSALPP 1906
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,413,181
Number of Sequences: 5004
Number of extensions: 13963
Number of successful extensions: 307
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 49
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 229
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 537243034
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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