Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP06_F_A12
         (881 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr ...    44   2e-05
SPAC9G1.13c |||histone acetyltransferase complex subunit Swc4 |S...    30   0.38 
SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schiz...    29   0.88 
SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit Bgs3|Schizo...    27   3.5  
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce...    26   6.2  

>SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 450

 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 41/118 (34%), Positives = 54/118 (45%)
 Frame = +3

Query: 252 DAVVEQDTLWRKLRHEADNFNKLKNVCSKEIGLKMKNKEPSGSEDEPVPSEIANNLVNLT 431
           D V+E    W  LR E DN NK  N   KEIGLKMK KE +        SE+     +LT
Sbjct: 33  DKVIEMYKEWVSLRFELDNTNKSINRVQKEIGLKMKAKEDA--------SELLEEKNSLT 84

Query: 432 GDNLKPLTVNQIKKVRVLIDNAISKNDEGLLAAEKARSAALREVGNHLHESVPVDDDE 605
                       ++ + LI+   +KN E L          +  +GN +H+SVPV  DE
Sbjct: 85  ------------ERKKNLIEQETAKNKEML--------NVVSSIGNIVHDSVPVSMDE 122



 Score = 39.5 bits (88), Expect = 6e-04
 Identities = 16/31 (51%), Positives = 25/31 (80%)
 Frame = +2

Query: 155 VLDLDLFRADKDGNPDKIRENQKKRFKDVAL 247
           +LD++LF+ +K GNP+ IRE+Q+KR  DV +
Sbjct: 1   MLDINLFQVEKGGNPEIIRESQRKRGADVGV 31


>SPAC9G1.13c |||histone acetyltransferase complex subunit Swc4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 411

 Score = 30.3 bits (65), Expect = 0.38
 Identities = 18/44 (40%), Positives = 24/44 (54%)
 Frame = +3

Query: 339 EIGLKMKNKEPSGSEDEPVPSEIANNLVNLTGDNLKPLTVNQIK 470
           EIG K K+K P+    E  P  I+  L +L G+N  PL + Q K
Sbjct: 16  EIGNKQKSKTPT----ERRPEGISRELYSLLGENSAPLAIYQKK 55


>SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 665

 Score = 29.1 bits (62), Expect = 0.88
 Identities = 16/38 (42%), Positives = 24/38 (63%)
 Frame = -3

Query: 264 RRLHPXRATSLNLFF*FSRILSGLPSLSARNKSKSRTM 151
           +RL+  R++  +++    R L GLPSLS RN  KS T+
Sbjct: 114 KRLNRSRSSKEDMYR--RRSLHGLPSLSRRNSKKSSTL 149


>SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit
           Bgs3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1826

 Score = 27.1 bits (57), Expect = 3.5
 Identities = 12/50 (24%), Positives = 23/50 (46%)
 Frame = -2

Query: 418 RLLAISEGTGSSSEPLGSLFFIFKPISLLHTFFSLLKLSASWRNFLHKVS 269
           + L +S+G+      + S +F+   I      +   + S +W +FLH  S
Sbjct: 376 KALLLSDGSRIMDADVASRYFLLADIQWQRVCYKSFRESRTWLHFLHNFS 425


>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 568

 Score = 26.2 bits (55), Expect = 6.2
 Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
 Frame = +3

Query: 435 DNLKPLTVNQIKKVRVLIDNAISKNDEG--LLAAEKARSAALRE 560
           +  +P+T N +KK +  ++ A  K  +     AA  AR+AA +E
Sbjct: 62  EEFEPITTNALKKAKKGVEQAAKKKAKAAEAEAAAAARAAAAKE 105


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,274,828
Number of Sequences: 5004
Number of extensions: 63000
Number of successful extensions: 189
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)