Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP05_F_O16
(877 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 108 1e-24
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 53 6e-08
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 48 1e-06
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 42 2e-04
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 41 3e-04
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 37 0.004
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 34 0.023
SPAC3A12.06c |||sodium/calcium exchanger |Schizosaccharomyces po... 27 2.7
SPBC23E6.01c ||SPBPJ758.01|RNA-binding protein, rrm type|Schizos... 27 2.7
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 6.1
SPBC1773.11c |mug89||CDC50 domain protein|Schizosaccharomyces po... 26 8.1
SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 26 8.1
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 108 bits (259), Expect = 1e-24
Identities = 75/148 (50%), Positives = 87/148 (58%)
Frame = +3
Query: 237 GVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITKLG*HGERSGTTPSTIELRVAPEG 416
G+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGI+ E+ ELRVAPE
Sbjct: 42 GIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWD-DMEKIWHHTFYNELRVAPEE 100
Query: 417 APXCWFTEAPPQTPKXNRGER*PRIMF*XXXNSPRPMXRRHSXAVXSXPGTPSGSYHRVS 596
P C TEAP PK NR E+ +I+F N+P + G +G
Sbjct: 101 HP-CLLTEAP-LNPKSNR-EKMTQIIF-ETFNAPAFYVAIQAVLSLYASGRTTGIV---- 152
Query: 597 CSNSGRWVSXTPLPIYEGYALPQAIFRL 680
+SG V+ T +PIYEGYALP AI RL
Sbjct: 153 -LDSGDGVTHT-VPIYEGYALPHAIMRL 178
Score = 101 bits (241), Expect = 2e-22
Identities = 64/131 (48%), Positives = 78/131 (59%), Gaps = 7/131 (5%)
Frame = +2
Query: 116 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG-------RDGRYGPERLLR 274
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G +D G E +
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 275 G*RGPEQERYPHSEVPHRARYHHQTGMTWRKIWHHTFYN*AACRPRRSTPVLVHRSPPSN 454
RG +YP + H ++ M KIWHHTFYN P P L+ + P N
Sbjct: 62 --RGILTLKYP---IEH-GIVNNWDDM--EKIWHHTFYNELRVAPEEH-PCLLTEA-PLN 111
Query: 455 PKGQQGRKMTQ 487
PK + KMTQ
Sbjct: 112 PKSNR-EKMTQ 121
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 52.8 bits (121), Expect = 6e-08
Identities = 21/38 (55%), Positives = 28/38 (73%)
Frame = +2
Query: 122 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 235
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHE 45
Score = 42.3 bits (95), Expect = 9e-05
Identities = 28/69 (40%), Positives = 34/69 (49%)
Frame = +3
Query: 240 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITKLG*HGERSGTTPSTIELRVAPEGA 419
VM QKD +VG EAQ+ RG+L ++ PIE GII E S +L PE
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 420 PXCWFTEAP 446
P TE P
Sbjct: 107 PLL-LTEPP 114
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 48.4 bits (110), Expect = 1e-06
Identities = 34/112 (30%), Positives = 50/112 (44%), Gaps = 2/112 (1%)
Frame = +2
Query: 128 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGRDGRYGPERLLRG*RGPEQER 301
A +V+DNG+G K G+A D+ PR FPSIVGRP R + + G + ++ G
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63
Query: 302 YPHSEVPHRARYHHQTGMTWRKIWHHTFYN*AACRPRRSTPVLVHRSPPSNP 457
+ P ++W +TF+ PR +L PP NP
Sbjct: 64 LLQVKYPMENGIIRDF-EEMNQLWDYTFFEKLKIDPRGRKILLT--EPPMNP 112
Score = 40.3 bits (90), Expect = 4e-04
Identities = 27/70 (38%), Positives = 37/70 (52%)
Frame = +3
Query: 261 KDSYVGDEAQSKRGILTLKYPIEHGIITKLG*HGERSGTTPSTIELRVAPEGAPXCWFTE 440
KD VGDEA++ R +L +KYP+E+GII + T +L++ P G TE
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYT-FFEKLKIDPRGRKIL-LTE 107
Query: 441 APPQTPKXNR 470
PP P NR
Sbjct: 108 -PPMNPVANR 116
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 41.5 bits (93), Expect = 2e-04
Identities = 23/60 (38%), Positives = 35/60 (58%), Gaps = 2/60 (3%)
Frame = +2
Query: 119 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGRDGR--YGPERLLRG*RGPE 292
D+V+A+V+D GS + GF+G+D P+ V PS G + DGR +G E + + G E
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG----EFSDGRRLFGEEYIYKSNPGME 64
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 40.7 bits (91), Expect = 3e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +2
Query: 134 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 217
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 36.7 bits (81), Expect = 0.004
Identities = 18/45 (40%), Positives = 28/45 (62%)
Frame = +2
Query: 95 TQSSKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 229
T S + +D+ LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 15 TPSFQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 34.3 bits (75), Expect = 0.023
Identities = 12/34 (35%), Positives = 23/34 (67%)
Frame = +2
Query: 119 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 220
+++ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPAC3A12.06c |||sodium/calcium exchanger |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 743
Score = 27.5 bits (58), Expect = 2.7
Identities = 18/43 (41%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
Frame = -3
Query: 449 RGGFGEPAXGCSFGGDTQLNCRRCGARSF-SMSSQFGDDTVLD 324
R GF E A G FGG T G SF S S G+D+V++
Sbjct: 643 RSGFPEMAMGGVFGGPTLNILIGIGISSFYSSISNHGNDSVIE 685
>SPBC23E6.01c ||SPBPJ758.01|RNA-binding protein, rrm
type|Schizosaccharomyces pombe|chr 2|||Manual
Length = 473
Score = 27.5 bits (58), Expect = 2.7
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = +3
Query: 141 STMAPACARPVSPATTRPAPSSRPSWVALATRGVMVGM 254
+++ PA + P A P P+ PS +AL TRG +GM
Sbjct: 398 TSLFPAMSLPPQ-AQFSPYPAVAPSPLALQTRGAPIGM 434
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 26.2 bits (55), Expect = 6.1
Identities = 15/38 (39%), Positives = 17/38 (44%)
Frame = -3
Query: 344 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHHAPG 231
GDD+VLD V +D L L AH D H G
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFHIAG 713
>SPBC1773.11c |mug89||CDC50 domain protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 396
Score = 25.8 bits (54), Expect = 8.1
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = +1
Query: 346 NWDDMEKDLAPHL 384
NWD E +LAPHL
Sbjct: 380 NWDSEENNLAPHL 392
>SPBC1683.09c |frp1||ferric-chelate reductase
Frp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 564
Score = 25.8 bits (54), Expect = 8.1
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +3
Query: 225 LATRGVMVGMGQKDSYVGDEAQSKRGILTLKYPI 326
L+ +M+G +Y+G AQ KR +LT + I
Sbjct: 160 LSQYAIMIGAIHGFAYIGLAAQGKRALLTARVTI 193
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,231,386
Number of Sequences: 5004
Number of extensions: 63975
Number of successful extensions: 205
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 202
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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