Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP05_F_O10
(882 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 272 4e-74
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 144 1e-35
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 134 2e-32
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 105 6e-24
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 71 2e-13
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 65 1e-11
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 64 2e-11
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 43 5e-05
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 35 0.013
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.12
SPAPB1A10.09 |ase1||microtubule-associated protein Ase1 |Schizos... 28 2.0
SPAC29A4.20 |||RNA polymerase II elongator complex, histone acet... 27 2.7
SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein ... 27 3.5
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 6.2
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 26 6.2
SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 26 6.2
SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 26 8.2
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 272 bits (667), Expect = 4e-74
Identities = 139/197 (70%), Positives = 147/197 (74%), Gaps = 1/197 (0%)
Frame = +2
Query: 257 PRIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFY 436
P IVGRPRH G+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFY
Sbjct: 32 PSIVGRPRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFY 91
Query: 437 NELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGY 616
NELRVAPEEHP LLTEAPLNPK+NREKMTQI+FETFN PA YVAIQAVLSLYASGRTTG
Sbjct: 92 NELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGI 151
Query: 617 RAGLRRRCLPHRAHLRRIRTPPRHPASGLSRSRPHRLPHEXS-SPSRGYSFXTTAXREIV 793
+ H + P H L + + RGY+F TTA REIV
Sbjct: 152 VLD-SGDGVTHTVPIYEGYALP-HAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIV 209
Query: 794 RDIKEXLCYVALDFXXE 844
RDIKE LCYVALDF E
Sbjct: 210 RDIKEKLCYVALDFEQE 226
Score = 87.4 bits (207), Expect = 2e-18
Identities = 39/41 (95%), Positives = 41/41 (100%)
Frame = +3
Query: 615 IVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 737
IVLDSGDGV+HTVPIYEGYALPHAI+RLDLAGRDLTDYLMK
Sbjct: 151 IVLDSGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMK 191
Score = 60.9 bits (141), Expect = 2e-10
Identities = 26/35 (74%), Positives = 31/35 (88%)
Frame = +1
Query: 169 KKLPRLVVDNGSGMCKAGFAGDDAPRAVFPSNRGK 273
+++ LV+DNGSGMCKAGFAGDDAPRAVFPS G+
Sbjct: 3 EEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGR 37
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 144 bits (350), Expect = 1e-35
Identities = 81/197 (41%), Positives = 111/197 (56%), Gaps = 1/197 (0%)
Frame = +2
Query: 257 PRIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFY 436
P VGR +H+ VM QKD +VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + +
Sbjct: 36 PTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYS 95
Query: 437 N-ELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTG 613
+ +L PEEHP+LLTE PL N+EK+ + +ET N PA+ ++Q VL+LYAS RTTG
Sbjct: 96 DQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTG 155
Query: 614 YRAGLRRRCLPHRAHLRRIRTPPRHPASGLSRSRPHRLPHEXSSPSRGYSFXTTAXREIV 793
L H + + P R + G+ ++A +EIV
Sbjct: 156 IVLECGDG-LTHSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIV 214
Query: 794 RDIKEXLCYVALDFXXE 844
R+IKE CYVA DF E
Sbjct: 215 REIKEKCCYVASDFRSE 231
Score = 57.2 bits (132), Expect = 3e-09
Identities = 22/39 (56%), Positives = 34/39 (87%)
Frame = +3
Query: 615 IVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYL 731
IVL+ GDG++H+VPIY+G+++P AI + ++ GRD+TDYL
Sbjct: 156 IVLECGDGLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYL 194
Score = 44.0 bits (99), Expect = 3e-05
Identities = 17/30 (56%), Positives = 23/30 (76%)
Frame = +1
Query: 184 LVVDNGSGMCKAGFAGDDAPRAVFPSNRGK 273
+ +DNGSG KAGFAGDD P+ +FP+ G+
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGR 41
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 134 bits (324), Expect = 2e-32
Identities = 78/200 (39%), Positives = 115/200 (57%), Gaps = 3/200 (1%)
Frame = +2
Query: 257 PRIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHT 430
P IVGRP R + + KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +T
Sbjct: 30 PSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYT 89
Query: 431 FYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTT 610
F+ +L++ P +LLTE P+NP ANREKM + MFE + +YVAIQAVLSLYA G ++
Sbjct: 90 FFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSS 149
Query: 611 GYRAGLRRRCLPHRAHLRRIRTPPRHPASGLSRSRPHRLPHEXSSPSR-GYSFXTTAXRE 787
G + H + H L + + S R GY+F TA E
Sbjct: 150 GVVVD-SGDGVTHIVPVYE-SVVLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFE 207
Query: 788 IVRDIKEXLCYVALDFXXEN 847
VR++KE LCYV+ D ++
Sbjct: 208 TVREMKEKLCYVSYDLELDH 227
Score = 57.2 bits (132), Expect = 3e-09
Identities = 24/40 (60%), Positives = 31/40 (77%)
Frame = +3
Query: 615 IVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLM 734
+V+DSGDGV+H VP+YE L H + RLD+AGRD T YL+
Sbjct: 151 VVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGRDATRYLI 190
Score = 35.9 bits (79), Expect = 0.008
Identities = 15/30 (50%), Positives = 21/30 (70%)
Frame = +1
Query: 184 LVVDNGSGMCKAGFAGDDAPRAVFPSNRGK 273
+V+DNG+G K G+A D+ PR FPS G+
Sbjct: 6 IVLDNGTGFVKVGYAKDNFPRFQFPSIVGR 35
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 105 bits (253), Expect = 6e-24
Identities = 47/96 (48%), Positives = 63/96 (65%), Gaps = 1/96 (1%)
Frame = +2
Query: 314 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 490
D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE P
Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132
Query: 491 LNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYAS 598
LNP NRE +IMFE+FN +Y+A+QAVL+L AS
Sbjct: 133 LNPPENRENTAEIMFESFNCAGLYIAVQAVLALAAS 168
Score = 47.6 bits (108), Expect = 2e-06
Identities = 19/38 (50%), Positives = 29/38 (76%)
Frame = +3
Query: 618 VLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYL 731
V+DSGDGV+H +P+ EGY + +I + LAGRD+T ++
Sbjct: 183 VVDSGDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFV 220
Score = 38.3 bits (85), Expect = 0.001
Identities = 14/26 (53%), Positives = 21/26 (80%)
Frame = +1
Query: 184 LVVDNGSGMCKAGFAGDDAPRAVFPS 261
+++DNG+G K G+AG+DAP VFP+
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPT 33
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 71.3 bits (167), Expect = 2e-13
Identities = 33/85 (38%), Positives = 48/85 (56%)
Frame = +2
Query: 356 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 535
+ +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 536 ETFNTPAMYVAIQAVLSLYASGRTT 610
E+ PA Y+A Q + +ASG+ T
Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGT 147
Score = 33.1 bits (72), Expect = 0.054
Identities = 13/30 (43%), Positives = 20/30 (66%)
Frame = +1
Query: 184 LVVDNGSGMCKAGFAGDDAPRAVFPSNRGK 273
+V+D GS + GF+G+D P+ V PS G+
Sbjct: 14 IVIDPGSKWTRIGFSGEDIPKCVLPSYCGE 43
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 64.9 bits (151), Expect = 1e-11
Identities = 33/114 (28%), Positives = 61/114 (53%), Gaps = 2/114 (1%)
Frame = +2
Query: 275 PRHQGVMVGMGQKDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELR 448
P + GV + ++ YV DE Q I + +K +GI+ +W+ W +L+
Sbjct: 37 PSYYGVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQ 96
Query: 449 VAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTT 610
V P E+ +++TE NP++ R+++ + FE + PA Y+ QAV +A+ ++T
Sbjct: 97 VNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKST 150
Score = 35.5 bits (78), Expect = 0.010
Identities = 15/43 (34%), Positives = 29/43 (67%), Gaps = 1/43 (2%)
Frame = +1
Query: 169 KKLPRLVVDNGSGMCKAGFAGDDAPRAVFPSNRG-KAPPSGRD 294
+++P LV+D GS + G+AG+++P + PS G ++ +GR+
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYGVRSDVTGRN 50
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 64.5 bits (150), Expect = 2e-11
Identities = 30/98 (30%), Positives = 56/98 (57%), Gaps = 1/98 (1%)
Frame = +2
Query: 302 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL 478
+ + + VG++ + G ++ + P E +++NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 479 TEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLY 592
TE NP R MT+++FE +N P++ I + S Y
Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFY 158
Score = 30.7 bits (66), Expect = 0.29
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +1
Query: 184 LVVDNGSGMCKAGFAGDDAPRAVF 255
LV+DNGS +AG+ G+ P+ VF
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVF 50
Score = 30.7 bits (66), Expect = 0.29
Identities = 13/41 (31%), Positives = 21/41 (51%)
Frame = +3
Query: 615 IVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMK 737
IVL+ G+ SH +P+ G + R+ G + YL+K
Sbjct: 168 IVLNLGNAASHVIPVLNGERILSEAKRISWGGSQSSSYLLK 208
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 43.2 bits (97), Expect = 5e-05
Identities = 25/81 (30%), Positives = 38/81 (46%), Gaps = 4/81 (4%)
Frame = +2
Query: 371 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 538
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 539 TFNTPAMYVAIQAVLSLYASG 601
P +A + ++ LYA G
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIG 192
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 35.1 bits (77), Expect = 0.013
Identities = 14/39 (35%), Positives = 25/39 (64%)
Frame = +3
Query: 615 IVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYL 731
+V+DSG +H +P+ + A+ R+D+ G+ LT+YL
Sbjct: 159 LVIDSGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYL 197
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 31.9 bits (69), Expect = 0.12
Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +2
Query: 431 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 574
+Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAPB1A10.09 |ase1||microtubule-associated protein Ase1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 731
Score = 27.9 bits (59), Expect = 2.0
Identities = 21/55 (38%), Positives = 26/55 (47%), Gaps = 1/55 (1%)
Frame = +1
Query: 559 VRRHPSRALAVRVRSYHRXSCWTPATVSP-TPCPSTKDTHSPTPSCVWT*PVATS 720
V R PS+ V V S + A SP TP P K+T +P+ S P ATS
Sbjct: 491 VNRTPSQRGRVAVPSTPSVRSASRAMTSPRTPLPRVKNTQNPSRSISAEPPSATS 545
>SPAC29A4.20 |||RNA polymerase II elongator complex, histone
acetyltransferase subunit |Schizosaccharomyces pombe|chr
1|||Manual
Length = 544
Score = 27.5 bits (58), Expect = 2.7
Identities = 19/70 (27%), Positives = 30/70 (42%), Gaps = 4/70 (5%)
Frame = +2
Query: 536 ETFNTPAMYVAIQAVLSLYASGRTTGYRAGLRRRCLPHRAHLRRIRTPPRHPASGLS--- 706
+ N A+ + I L S R T A + + +R++R P ASG++
Sbjct: 29 KVINLNALKMRISKKHQLSESPRLTDIIAAIPPDAYLKESLMRKLRAKPVRTASGIAVVA 88
Query: 707 -RSRPHRLPH 733
+PHR PH
Sbjct: 89 VMCKPHRCPH 98
>SPAC22A12.08c |||cardiolipin synthase/ hydrolase fusion protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 570
Score = 27.1 bits (57), Expect = 3.5
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +2
Query: 14 PPHYREFFKISIPRSELGSLRCTAITKLCNSKLI 115
P +R FF +IP +E L+ T I+K+C + L+
Sbjct: 511 PKTFRRFFDFAIPTTE---LKPTRISKVCRNGLL 541
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 6.2
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2
Query: 473 LLTEAPLNPKANREKMTQIMFETFN 547
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 26.2 bits (55), Expect = 6.2
Identities = 13/40 (32%), Positives = 16/40 (40%)
Frame = -2
Query: 317 SPSVPYRPSRPDGGAFPRFEGNTARGASSPAKPALHIPEP 198
+P VP P RP P R A AL +P+P
Sbjct: 538 APEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSVPQP 577
>SPAC20G8.05c |cdc15||cell division control protein
Cdc15|Schizosaccharomyces pombe|chr 1|||Manual
Length = 927
Score = 26.2 bits (55), Expect = 6.2
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = -2
Query: 338 VPHLLHKSPSVPYRPSRPDGGA 273
+ L+ K+PS PY SRP A
Sbjct: 306 IDQLISKAPSYPYSSSRPSASA 327
>SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase
Tor2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2337
Score = 25.8 bits (54), Expect = 8.2
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = -3
Query: 367 FEGQDTSFALCLISYIRVLLSHTDHHA 287
+EG+DT+ +Y+R++L TD A
Sbjct: 74 YEGEDTTRITRFANYLRIILPGTDQKA 100
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,606,481
Number of Sequences: 5004
Number of extensions: 78354
Number of successful extensions: 246
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 211
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 243
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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