Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP05_F_O06
(930 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 170 2e-43
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 101 1e-22
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 88 2e-18
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 81 2e-16
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 71 2e-13
SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe... 48 2e-06
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 29 1.2
SPBC32F12.10 |||phosphoglucomutase |Schizosaccharomyces pombe|ch... 27 2.8
SPBC32F12.02 |rec14||recombination protein Rec14|Schizosaccharom... 27 2.8
SPBC8D2.11 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 2.8
SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|ch... 27 3.8
SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl modifica... 26 6.6
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 26 6.6
SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces p... 26 6.6
SPBC8D2.12c |||mitochondrial DNA binding protein |Schizosaccharo... 26 8.7
>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 360
Score = 170 bits (414), Expect = 2e-43
Identities = 76/183 (41%), Positives = 113/183 (61%)
Frame = +2
Query: 167 NDDEVLIKIDCVGICGSDVKLYSTGTCGADVIDKPIVIGHEGAGTVVKVGDKVSSLRVGD 346
+D +V + I GICGSDV + G G ++ KP+++GHE AG VV+VG VSSL+ GD
Sbjct: 28 DDHQVKVAIKATGICGSDVHYWKEGGIGDFILKKPMILGHESAGVVVEVGKGVSSLKPGD 87
Query: 347 RVAIEPTQPCRSCELCKRGKYNLCVEPRYCSSMGAPGNLCRYYKHVADFCHKLPDNLTME 526
VA+EP CR C+ C+ G+YNLC + ++ G L YY DFC KLP +++E
Sbjct: 88 PVAVEPGCVCRLCDYCRSGRYNLCPHMEFAATPPYDGTLRTYYITTEDFCTKLPKQISVE 147
Query: 527 EGAAVQPLAIVIHACNRAKITLGSKIVILGAGPIGILCAMSAKAMGASKIILTDVVQSRL 706
EGA +P+++ +HA R + GS+++++G G +G+L AKA GA I+ D SR+
Sbjct: 148 EGALFEPMSVAVHAMTRGNLKCGSRVLVMGCGTVGLLMMAVAKAYGAIDIVAVDASPSRV 207
Query: 707 DAA 715
+ A
Sbjct: 208 EFA 210
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 101 bits (243), Expect = 1e-22
Identities = 77/231 (33%), Positives = 115/231 (49%), Gaps = 23/231 (9%)
Frame = +2
Query: 176 EVLIKIDCVGIC-GSDVKLYSTGTCGADVIDKPIVIGHEGAGTVVKVGDKVSSLRVGDRV 352
+V++K IC GSD ++S G I+K ++GHE G V + GD+V++L +GDRV
Sbjct: 63 DVIVKTTACTICSGSDSHIFSGEMPG---IEKGAILGHESCGIVAEKGDEVNNLEIGDRV 119
Query: 353 AIEPTQPCRSCELCKRGKYNLC--------VEPRYCSSMGA-----------PGNLCRYY 475
I C C CKR +Y C ++ Y S A PG Y
Sbjct: 120 VIAFDLACGQCSFCKRHEYAACDTTNDSKLMDVNYGSHHSAIFGYTKLLGDVPGCQAEYI 179
Query: 476 K-HVADF-CHKLPDNLTMEEGAAVQP-LAIVIHACNRAKITLGSKIVILGAGPIGILCAM 646
+ A+ C KLPD++ EG + L +HAC ++ G + I G GPIG+
Sbjct: 180 RVPFAEINCCKLPDDIPDSEGLFMSDVLCTSLHACTLGEVKKGDTVAIWGMGPIGLYAGR 239
Query: 647 SAKAMGASKIILTDVVQSRLDAALELGADTVLLVRREYTDEEVVKKIVKLL 799
A+ +GASK+I +VV R++ A + TV + R E +D V KKI++L+
Sbjct: 240 WAQILGASKVIGIEVVPERIELARQKFGFTV-IDRNEVSD--VPKKIMELV 287
>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
Adh1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 350
Score = 87.8 bits (208), Expect = 2e-18
Identities = 64/207 (30%), Positives = 99/207 (47%), Gaps = 4/207 (1%)
Frame = +2
Query: 173 DEVLIKIDCVGICGSDVK-LYSTGTCGADVIDKPIVIGHEGAGTVVKVGDKVSSLRVGDR 349
DEVL+ I G+C +D+ L A + P++ GHEGAG VVKVG V+ L++GDR
Sbjct: 34 DEVLVNIKYTGVCHTDLHALQGDWPLPAKM---PLIGGHEGAGVVVKVGAGVTRLKIGDR 90
Query: 350 VAIE-PTQPCRSCELCKRGKYNLCVEPRYCSSMGAPGNLCRYYKHVADFCHKLPDNLTME 526
V ++ C +CE C + + +C + S G Y A +P+++ +E
Sbjct: 91 VGVKWMNSSCGNCEYCMKAEETICPHIQ-LSGYTVDGTFQHYCIANATHATIIPESVPLE 149
Query: 527 EGAAVQPLAIVIH-ACNRAKITLGSKIVILGA-GPIGILCAMSAKAMGASKIILTDVVQS 700
A + I + A +K+ G I I GA G +G L AKAM A +++ D
Sbjct: 150 VAAPIMCAGITCYRALKESKVGPGEWICIPGAGGGLGHLAVQYAKAM-AMRVVAIDTGDD 208
Query: 701 RLDAALELGADTVLLVRREYTDEEVVK 781
+ + GA+ L ++E E VK
Sbjct: 209 KAELVKSFGAEVFLDFKKEADMIEAVK 235
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 81.4 bits (192), Expect = 2e-16
Identities = 67/226 (29%), Positives = 107/226 (47%), Gaps = 23/226 (10%)
Frame = +2
Query: 122 EPTTSESRKFQCPRINDDEVLIKIDCVGICGSDVKLYSTGTCGADVIDK-PIVIGHEGAG 298
EP + E + P+ + EV +K+D +C +D S G D PIV+GHEGAG
Sbjct: 20 EPLSIEDIQVAPPKAH--EVRVKVDWSAVCHTDAYTLS----GVDPEGAFPIVLGHEGAG 73
Query: 299 TVVKVGDKVSSLRVGDRVAIEPTQPCRSCELCKRGKYNLCVEPRYCSSMG-APGNL---- 463
V +G+ V ++R GD V + T C+ C+ C+ GK NLC + R G P
Sbjct: 74 IVESIGEGVINVRPGDHVILLYTPECKECKFCRSGKTNLCSKIRETQGRGLMPDGTSRFS 133
Query: 464 CR-----YYKHVADFC-HKLPDNLTMEEGAAVQPL-AIVIHAC----------NRAKITL 592
CR +Y + F + + ++++ + PL +I + C + AK+
Sbjct: 134 CRDKTLLHYMGCSSFSQYTVVADISLVAISHSAPLRSICLLGCGVTTGFGAVTHSAKVES 193
Query: 593 GSKIVILGAGPIGILCAMSAKAMGASKIILTDVVQSRLDAALELGA 730
GS + ++G G +G+ A A GAS+II D+ + A + GA
Sbjct: 194 GSTVAVVGCGCVGLAAMQGAVAAGASRIIAIDINADKEVYAKKFGA 239
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 70.9 bits (166), Expect = 2e-13
Identities = 62/225 (27%), Positives = 102/225 (45%), Gaps = 23/225 (10%)
Frame = +2
Query: 125 PTTSESRKFQCPRINDDEVLIKIDCVGICGSDVKLYSTGTCGADVIDK-PIVIGHEGAGT 301
P + E+ + PR++ EV IKI G+C +D S G D P+++GHEGAG
Sbjct: 24 PLSIENVQVFPPRVH--EVRIKIVNSGVCHTDAYTLS----GKDPEGLFPVILGHEGAGI 77
Query: 302 VVKVGDKVSSLRVGDRVAIEPTQPCRSCELCKRGKYNLC----------VEPRYCSSMGA 451
V VG +V++++VGD V T C++C+ CK GK NLC + P S
Sbjct: 78 VESVGPQVTTVQVGDPVIALYTPECKTCKFCKSGKTNLCGRIRTTQGKGLMPDGTSRFSC 137
Query: 452 PGNLCRYYKHVADFC-HKLPDNLTMEEGAAVQPL-AIVIHACN----------RAKITLG 595
GN ++ + F + + ++++ + PL ++ + C A I G
Sbjct: 138 NGNTLLHFMGCSTFSEYTVVADISVVAIERLAPLDSVCLLGCGITTGYGAATITADIKEG 197
Query: 596 SKIVILGAGPIGILCAMSAKAMGASKIILTDVVQSRLDAALELGA 730
+ + G G +G+ A A +I DV + + A+ GA
Sbjct: 198 DSVAVFGLGSVGLAVIQGAVKKRAGRIFGIDVNPEKKNWAMSFGA 242
>SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 346
Score = 48.0 bits (109), Expect = 2e-06
Identities = 49/203 (24%), Positives = 88/203 (43%), Gaps = 8/203 (3%)
Frame = +2
Query: 146 KFQCP-RINDDEVLIKIDCVGICGSDVKLYSTGTCGADVIDKPIVIGHEGAGTVVKVGDK 322
+++ P ++N EVL+K+ + D+ + + G + P+V G +GAG + KVG+
Sbjct: 21 EYEVPQKLNPGEVLVKLKAASLNYRDL-IITKGLYPLP-LQLPVVPGSDGAGIIEKVGED 78
Query: 323 VSSLRVGDRVAIEPTQPCRSCELCKRGKYNLCVEPRYCSSMGAPGNLCRYYKHVADFCHK 502
V GD V C + S++G + C + K+ H
Sbjct: 79 VEGFEKGDSVV---------CNFFTNYLDGTPTDFATHSALGGTRDGC-FQKYAVLPAHA 128
Query: 503 L---PDNLTMEEGAAVQPLAIV----IHACNRAKITLGSKIVILGAGPIGILCAMSAKAM 661
L P NL+ EE A + A+ + ++ G+ +++LG G + A A
Sbjct: 129 LVHAPKNLSFEEIATLPCAAVTAWNGLFGSKEHQVKPGNNVLVLGTGGVSTFALQFALAA 188
Query: 662 GASKIILTDVVQSRLDAALELGA 730
GA+ + +T +L+ A +LGA
Sbjct: 189 GAN-VTVTSSSDEKLEFAKKLGA 210
>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
Length = 582
Score = 28.7 bits (61), Expect = 1.2
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +2
Query: 239 GTCGADVIDKPIVIGHEGAGTVVKVGDKVSSLR 337
G+CG+ + K I +GAG VKV D+ +R
Sbjct: 349 GSCGSVTVTKEDTIIMKGAGDHVKVNDRCEQIR 381
>SPBC32F12.10 |||phosphoglucomutase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 554
Score = 27.5 bits (58), Expect = 2.8
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = +2
Query: 422 EPRYCSSMGAPGNLCRYYKHVADFCHKLPD-NLT 520
E +C +G P ++C+ K + DF PD NLT
Sbjct: 224 EALFCKELGMPSSVCQNCKPLPDFGGGHPDPNLT 257
>SPBC32F12.02 |rec14||recombination protein
Rec14|Schizosaccharomyces pombe|chr 2|||Manual
Length = 302
Score = 27.5 bits (58), Expect = 2.8
Identities = 21/96 (21%), Positives = 39/96 (40%), Gaps = 5/96 (5%)
Frame = +2
Query: 314 GDKVSSLRVGDRVAI-EPTQPCRSCELCKRGKYNLCVE----PRYCSSMGAPGNLCRYYK 478
G ++ V R+A+ + C+ EL +GK+ LC++ R+ S G L
Sbjct: 115 GQTIAFTSVDGRIAVWDNPSDCKISELDTKGKFGLCIDYSPNGRFIVSGHQTGQLFLIST 174
Query: 479 HVADFCHKLPDNLTMEEGAAVQPLAIVIHACNRAKI 586
H L + + A P + ++ A +K+
Sbjct: 175 ETGRLFHVLSGHTSPVRSVAFSPGSTLLAAAGDSKM 210
>SPBC8D2.11 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 122
Score = 27.5 bits (58), Expect = 2.8
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = -3
Query: 811 GRSPEQLHNFFYNLLVSVLPTD 746
GR PEQL NF + V VL T+
Sbjct: 34 GRLPEQLENFSFENFVEVLDTE 55
>SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 815
Score = 27.1 bits (57), Expect = 3.8
Identities = 15/50 (30%), Positives = 24/50 (48%)
Frame = +2
Query: 326 SSLRVGDRVAIEPTQPCRSCELCKRGKYNLCVEPRYCSSMGAPGNLCRYY 475
S + G +VA + R+C++C+R K C R C + A C Y+
Sbjct: 12 SKILEGAKVATRRRRVTRACDMCRRKKIK-CDGLRPCKNCKAGKLECTYH 60
>SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl
modification enzyme|Schizosaccharomyces pombe|chr
2|||Manual
Length = 666
Score = 26.2 bits (55), Expect = 6.6
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = +2
Query: 602 IVILGAGPIGILCAMSAKAMGASKIILT 685
+V++G G G+ A +A +GA +LT
Sbjct: 19 VVVIGGGHAGVEAAAAASRLGAKTTLLT 46
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 26.2 bits (55), Expect = 6.6
Identities = 15/44 (34%), Positives = 28/44 (63%), Gaps = 2/44 (4%)
Frame = +2
Query: 599 KIVILGAGPIGILCAMSAKAMG-ASKIILTDVVQSRLDA-ALEL 724
KIVI+GAG +G A + G A++I++ D+ + + + A++L
Sbjct: 22 KIVIVGAGNVGSTTAFTLLLSGLAAEIVIIDLNKKKAEGEAMDL 65
>SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 505
Score = 26.2 bits (55), Expect = 6.6
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 2/42 (4%)
Frame = -2
Query: 812 RAVTRATSQFFLQPPRQCTPD--GRGGRYQLPTPVLRLSVTE 693
RA R TS + PP + TPD G G +QL L +S+ +
Sbjct: 259 RAAERNTSLKVVIPPAELTPDMIGLSGVHQLGNTSLAVSLVQ 300
>SPBC8D2.12c |||mitochondrial DNA binding protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 293
Score = 25.8 bits (54), Expect = 8.7
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +2
Query: 704 LDAALELGADTVLLVRREYTDEE 772
LD A+E GA+ ++ +EY DEE
Sbjct: 185 LDDAIEAGAEDIVNRPKEYIDEE 207
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,441,019
Number of Sequences: 5004
Number of extensions: 68658
Number of successful extensions: 220
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 215
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 471335896
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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