Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP05_F_L09
(923 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 142 8e-35
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 59 1e-09
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 55 1e-08
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 38 0.003
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 34 0.025
SPCC70.10 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||M... 29 0.93
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 27 2.8
SPCC1020.12c ||SPCC14G10.06|xap-5-like protein|Schizosaccharomyc... 27 2.8
SPAC3C7.07c |||arginine-tRNA protein transferase |Schizosaccharo... 27 4.9
SPBC3H7.01 |spo14|stl1, SPBP16F5.01c|WD repeat protein Spo14|Sch... 27 4.9
SPAC20H4.03c |tfs1||transcription elongation factor TFIIS |Schiz... 27 4.9
SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 26 8.6
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 142 bits (343), Expect = 8e-35
Identities = 97/205 (47%), Positives = 110/205 (53%), Gaps = 1/205 (0%)
Frame = +1
Query: 187 GMCKAGFAGDDAPRAVFRPSWVALATR-GVMVGMGQKDSYVGDEAQSKRGILTLKYPIEH 363
GMCKAGFAGDDAPRAVF PS V G+MVGMGQKDSYVGDEAQSKRGILTLKYPIEH
Sbjct: 15 GMCKAGFAGDDAPRAVF-PSIVGRPRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEH 73
Query: 364 GYHHQLGMTWRRSGHHTFYNELRVAPEGAPXWLLTEAPPQXPRXTGEKDDPDHV*DLQLP 543
G + + HHTFYNELRVAPE P LLTEA P P+ EK P
Sbjct: 74 GIVNNWD-DMEKIWHHTFYNELRVAPEEHPC-LLTEA-PLNPKSNREKMTQIIFETFNAP 130
Query: 544 RPCTSPXQGRALXCTPPXXXXXXXXXXXXWCLPHRTHLRRLXSGPXPSSVLDLGWSRXDR 723
+ +L + + H + + P LDL
Sbjct: 131 AFYVAIQAVLSLYASGRTTGIVLDSGDG---VTHTVPIYEGYALPHAIMRLDLAGRDLTD 187
Query: 724 LPHEXXTERGYSFTTTAXREIVRDI 798
+ ERGY+F+TTA REIVRDI
Sbjct: 188 YLMKILMERGYTFSTTAEREIVRDI 212
Score = 68.9 bits (161), Expect = 9e-13
Identities = 49/105 (46%), Positives = 53/105 (50%), Gaps = 1/105 (0%)
Frame = +2
Query: 506 KMTQIMFETFNSPGHVRRHXXXXXXXXXXXXXPXGIVLDSGDGVSHTVPIYEGXALAQXH 685
KMTQI+FETFN+P GIVLDSGDGV+HTVPIYEG AL
Sbjct: 118 KMTQIIFETFNAPAFYVA--IQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAI 175
Query: 686 PPFWTWAGRXLTDYLMXI-XPRGVTRSPPPLXGKSFVTSXKTLLC 817
AGR LTDYLM I RG T S + V K LC
Sbjct: 176 MRL-DLAGRDLTDYLMKILMERGYTFS--TTAEREIVRDIKEKLC 217
Score = 54.0 bits (124), Expect = 3e-08
Identities = 66/223 (29%), Positives = 83/223 (37%), Gaps = 1/223 (0%)
Frame = +3
Query: 237 PSIVGRPRHQGRDGRYGPERLLRG*RGPEQERYPHSEVPHRARVSSPTGDDMEKIWPPHL 416
PSIVGRPRH G G + G + + P + + DDMEKIW
Sbjct: 32 PSIVGRPRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVN-NWDDMEKIWHHTF 90
Query: 417 LQ*AACRPRRSTPVAAHRSPPSXPKXNRGER*PRSCLRPSTPPAMYVAXPGPCSRLYASG 596
P + P PK NR E+ + PA YVA S LYASG
Sbjct: 91 YNELRVAPEEHPCLLTEA--PLNPKSNR-EKMTQIIFETFNAPAFYVAIQAVLS-LYASG 146
Query: 597 SYXRVSCSTPEMVSPTPYPSTKVXLWPXAILRFGLGLVAX*PTTS*XSHREGLLVHHHR* 776
+ + + V+ T P + P AI+R L G
Sbjct: 147 RTTGIVLDSGDGVTHT-VPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAE 205
Query: 777 XGNRS*HXEKXCYVALXFEQEM-XXXXASXPSRSLXTPDGQVI 902
EK CYVAL FEQE+ +S +S PDGQVI
Sbjct: 206 REIVRDIKEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVI 248
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 58.8 bits (136), Expect = 1e-09
Identities = 37/97 (38%), Positives = 47/97 (48%)
Frame = +1
Query: 187 GMCKAGFAGDDAPRAVFRPSWVALATRGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHG 366
G KAGFAGDD P+ +F + VM QKD +VG EAQ+ RG+L ++ PIE G
Sbjct: 19 GFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKIQRPIERG 78
Query: 367 YHHQLGMTWRRSGHHTFYNELRVAPEGAPXWLLTEAP 477
+ +L PE P LLTE P
Sbjct: 79 IIQNWSDMEEIWSYIYSDQQLNTLPEEHPL-LLTEPP 114
Score = 43.6 bits (98), Expect = 4e-05
Identities = 35/126 (27%), Positives = 49/126 (38%)
Frame = +2
Query: 353 PSSTGIITNWG*HGEDLATTPSTMSCVSPPKEHXXXXXXXXXXXXXXXXXRKMTQIMFET 532
P GII NW E + S + P+EH K+ + +ET
Sbjct: 74 PIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNK--EKIAEYFYET 131
Query: 533 FNSPGHVRRHXXXXXXXXXXXXXPXGIVLDSGDGVSHTVPIYEGXALAQXHPPFWTWAGR 712
N P GIVL+ GDG++H+VPIY+G ++ GR
Sbjct: 132 LNVPA--LSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQ-EEIGGR 188
Query: 713 XLTDYL 730
+TDYL
Sbjct: 189 DVTDYL 194
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 55.2 bits (127), Expect = 1e-08
Identities = 41/113 (36%), Positives = 56/113 (49%), Gaps = 6/113 (5%)
Frame = +1
Query: 187 GMCKAGFAGDDAPRAVFRPSWVALATRGVMVGMGQ---KDSYVGDEAQSKRGILTLKYPI 357
G K G+A D+ PR F PS V G KD VGDEA++ R +L +KYP+
Sbjct: 13 GFVKVGYAKDNFPRFQF-PSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRSLLQVKYPM 71
Query: 358 EHGY---HHQLGMTWRRSGHHTFYNELRVAPEGAPXWLLTEAPPQXPRXTGEK 507
E+G ++ W +TF+ +L++ P G LLTE PP P EK
Sbjct: 72 ENGIIRDFEEMNQLW----DYTFFEKLKIDPRGRKI-LLTE-PPMNPVANREK 118
Score = 39.5 bits (88), Expect = 7e-04
Identities = 21/47 (44%), Positives = 27/47 (57%)
Frame = +2
Query: 608 GIVLDSGDGVSHTVPIYEGXALAQXHPPFWTWAGRXLTDYLMXIXPR 748
G+V+DSGDGV+H VP+YE L AGR T YL+ + R
Sbjct: 150 GVVVDSGDGVTHIVPVYESVVLNHLVGRL-DVAGRDATRYLISLLLR 195
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 37.5 bits (83), Expect = 0.003
Identities = 19/77 (24%), Positives = 33/77 (42%)
Frame = +2
Query: 509 MTQIMFETFNSPGHVRRHXXXXXXXXXXXXXPXGIVLDSGDGVSHTVPIYEGXALAQXHP 688
MT+++FE +N+P GIVL+ G+ SH +P+ G +
Sbjct: 134 MTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNGERILSEAK 193
Query: 689 PFWTWAGRXLTDYLMXI 739
+W G + YL+ +
Sbjct: 194 RI-SWGGSQSSSYLLKL 209
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 34.3 bits (75), Expect = 0.025
Identities = 16/44 (36%), Positives = 25/44 (56%)
Frame = +2
Query: 608 GIVLDSGDGVSHTVPIYEGXALAQXHPPFWTWAGRXLTDYLMXI 739
G V+DSGDGV+H +P+ EG + AGR +T ++ +
Sbjct: 181 GTVVDSGDGVTHIIPVAEGYVIGSSIKTM-PLAGRDVTYFVQSL 223
Score = 31.5 bits (68), Expect = 0.17
Identities = 37/125 (29%), Positives = 50/125 (40%), Gaps = 24/125 (19%)
Frame = +1
Query: 187 GMCKAGFAGDDAPRAVFRPSWVALATRGVMVG---------MGQK--------------D 297
G K G+AG+DAP VF P+ +A + G G M K D
Sbjct: 15 GYSKLGYAGNDAPSYVF-PTVIATRSAGASSGPAVSSKPSYMASKGSGHLSSKRATEDLD 73
Query: 298 SYVGDEAQSKRGI-LTLKYPIEHGYHHQLGMTWRRSGHHTFYNELRVAPEGAPXWLLTEA 474
++G++A K +L YPI HG R + + LR PE +LLTE
Sbjct: 74 FFIGNDALKKASAGYSLDYPIRHGQIENWD-HMERFWQQSLFKYLRCEPED-HYFLLTEP 131
Query: 475 PPQXP 489
P P
Sbjct: 132 PLNPP 136
>SPCC70.10 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 155
Score = 29.1 bits (62), Expect = 0.93
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +2
Query: 185 PACARPVSPATTRPAPSSVHRGSPSPP 265
PA RP++ A RP ++V R SP+ P
Sbjct: 39 PATTRPMAEARARPGATAVPRRSPTSP 65
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 27.5 bits (58), Expect = 2.8
Identities = 17/59 (28%), Positives = 24/59 (40%)
Frame = +2
Query: 185 PACARPVSPATTRPAPSSVHRGSPSPPGA*WSVWARKTPTWVTRPRAREVSSL*STPSS 361
PA V+PA PAP+ + P PP S+ P +T + + S P S
Sbjct: 721 PAIKPQVTPAPPTPAPTPAVKHHPPPPPVRSSISPSMPPAPLTHANSSTPMNYVSQPES 779
>SPCC1020.12c ||SPCC14G10.06|xap-5-like protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 288
Score = 27.5 bits (58), Expect = 2.8
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = +3
Query: 600 YXRVSCSTPEMVSPTPYPS 656
+ ++SCS+PEMV+ T PS
Sbjct: 219 FDKLSCSSPEMVATTQLPS 237
>SPAC3C7.07c |||arginine-tRNA protein transferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 361
Score = 26.6 bits (56), Expect = 4.9
Identities = 8/18 (44%), Positives = 13/18 (72%)
Frame = +1
Query: 364 GYHHQLGMTWRRSGHHTF 417
GY+ + + WRRSGH+ +
Sbjct: 10 GYNRLIDLGWRRSGHYLY 27
>SPBC3H7.01 |spo14|stl1, SPBP16F5.01c|WD repeat protein
Spo14|Schizosaccharomyces pombe|chr 2|||Manual
Length = 395
Score = 26.6 bits (56), Expect = 4.9
Identities = 16/44 (36%), Positives = 21/44 (47%)
Frame = -2
Query: 652 GYGVGDTISGVEHDTRWYXPEAYXREHGPGMATYMAGGVEGLKH 521
G G G T SGV++ + E Y E G G ++ G LKH
Sbjct: 24 GGGGGTTKSGVKNKLKLLSYEDYEPEVGEGHTKFIEHGEIELKH 67
>SPAC20H4.03c |tfs1||transcription elongation factor TFIIS
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 293
Score = 26.6 bits (56), Expect = 4.9
Identities = 11/25 (44%), Positives = 12/25 (48%)
Frame = -2
Query: 169 YQAQQHSSFAHSLNFCVCFVFGERW 95
YQ Q S+ FC C V G RW
Sbjct: 266 YQMQTRSADEPMTTFCECTVCGNRW 290
>SPBC1683.09c |frp1||ferric-chelate reductase
Frp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 564
Score = 25.8 bits (54), Expect = 8.6
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +1
Query: 256 LATRGVMVGMGQKDSYVGDEAQSKRGILTLKYPI 357
L+ +M+G +Y+G AQ KR +LT + I
Sbjct: 160 LSQYAIMIGAIHGFAYIGLAAQGKRALLTARVTI 193
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,629,491
Number of Sequences: 5004
Number of extensions: 76888
Number of successful extensions: 216
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 208
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 467341524
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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