BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP05_F_J21
(965 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 33 0.060
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.18
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 3.0
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 5.2
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 26 6.9
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 6.9
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 22 9.9
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 33.1 bits (72), Expect = 0.060
Identities = 18/49 (36%), Positives = 18/49 (36%), Gaps = 6/49 (12%)
Frame = +2
Query: 635 PXPPPPXXXXXXXXXXXPPPPPXKKXGG------XXGGGGXXXPPPPPP 763
P PPP P PPP GG G G PPPPPP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 32.7 bits (71), Expect = 0.080
Identities = 16/43 (37%), Positives = 16/43 (37%)
Frame = +2
Query: 635 PXPPPPXXXXXXXXXXXPPPPPXKKXGGXXGGGGXXXPPPPPP 763
P P P PPPPP G G PPPPPP
Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPPPPPG---VAGAGPPPPPPPPP 783
Score = 30.7 bits (66), Expect = 0.32
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +2
Query: 635 PXPPPPXXXXXXXXXXXPPPPPXKKXGG 718
P PPPP PPPPP GG
Sbjct: 762 PPPPPPPGVAGAGPPPPPPPPPAVSAGG 789
Score = 27.9 bits (59), Expect = 2.3
Identities = 11/28 (39%), Positives = 11/28 (39%)
Frame = +2
Query: 635 PXPPPPXXXXXXXXXXXPPPPPXKKXGG 718
P PPPP PPPPP G
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPPAVSAG 788
Score = 26.2 bits (55), Expect = 6.9
Identities = 13/39 (33%), Positives = 13/39 (33%)
Frame = +2
Query: 641 PPPPXXXXXXXXXXXPPPPPXKKXGGXXGGGGXXXPPPP 757
PPPP PPPPP GG P P
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPP--AVSAGGSRYYAPAP 797
Score = 26.2 bits (55), Expect = 6.9
Identities = 12/41 (29%), Positives = 12/41 (29%)
Frame = +2
Query: 635 PXPPPPXXXXXXXXXXXPPPPPXKKXGGXXGGGGXXXPPPP 757
P PPPP PPPP G P P
Sbjct: 763 PPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAPQAEPEP 803
Score = 25.8 bits (54), Expect = 9.1
Identities = 10/21 (47%), Positives = 10/21 (47%)
Frame = -3
Query: 756 GGGGXXXPPPPXXPPXFXXGG 694
G G PPPP PP GG
Sbjct: 769 GVAGAGPPPPPPPPPAVSAGG 789
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 31.5 bits (68), Expect = 0.18
Identities = 13/32 (40%), Positives = 16/32 (50%), Gaps = 4/32 (12%)
Frame = +2
Query: 686 PPPPPXKKXGG----XXGGGGXXXPPPPPPXK 769
PPPPP ++ G G PPPPPP +
Sbjct: 313 PPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPR 344
Score = 29.5 bits (63), Expect = 0.74
Identities = 13/28 (46%), Positives = 13/28 (46%), Gaps = 2/28 (7%)
Frame = +2
Query: 686 PPPPPXKKXGGXXG--GGGXXXPPPPPP 763
PPPPP G G PPPPPP
Sbjct: 339 PPPPPRSNAAGSIPLPPQGRSAPPPPPP 366
Score = 27.9 bits (59), Expect = 2.3
Identities = 10/29 (34%), Positives = 12/29 (41%)
Frame = +2
Query: 686 PPPPPXKKXGGXXGGGGXXXPPPPPPXKK 772
P PPP + PPPPPP +
Sbjct: 291 PLPPPSSRVSAAALAANKKRPPPPPPPSR 319
Score = 26.6 bits (56), Expect = 5.2
Identities = 9/28 (32%), Positives = 12/28 (42%)
Frame = +2
Query: 689 PPPPXKKXGGXXGGGGXXXPPPPPPXKK 772
PPP + PPPPPP ++
Sbjct: 293 PPPSSRVSAAALAANKKRPPPPPPPSRR 320
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 27.5 bits (58), Expect = 3.0
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +2
Query: 635 PXPPPPXXXXXXXXXXXPPPPP 700
P PPPP PPPPP
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPP 30
Score = 27.5 bits (58), Expect = 3.0
Identities = 9/13 (69%), Positives = 9/13 (69%)
Frame = -1
Query: 509 PAPPPPPXXFXPP 471
P PPPPP F PP
Sbjct: 9 PPPPPPPPGFEPP 21
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 26.6 bits (56), Expect = 5.2
Identities = 15/42 (35%), Positives = 15/42 (35%)
Frame = -3
Query: 759 GGGGGXXXPPPPXXPPXFXXGGGGGXXXXXXXXXXXGGGGXG 634
GG GG PPP GG GG GGG G
Sbjct: 191 GGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGG 232
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 26.2 bits (55), Expect = 6.9
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +2
Query: 716 GXXGGGGXXXPPPPPP 763
G GG PPPPPP
Sbjct: 298 GDSANGGLPPPPPPPP 313
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.2 bits (55), Expect = 6.9
Identities = 9/13 (69%), Positives = 9/13 (69%)
Frame = -1
Query: 509 PAPPPPPXXFXPP 471
PAPPPPP PP
Sbjct: 236 PAPPPPPPPTLPP 248
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 21.8 bits (44), Expect(2) = 9.9
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = -2
Query: 520 FXXGPPPPPP 491
F PPPPPP
Sbjct: 944 FPPPPPPPPP 953
Score = 21.8 bits (44), Expect(2) = 9.9
Identities = 8/16 (50%), Positives = 8/16 (50%)
Frame = -2
Query: 508 PPPPPPXXFFXPPXGG 461
PPPPPP GG
Sbjct: 945 PPPPPPPPPLVSAAGG 960
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,646,760
Number of Sequences: 5004
Number of extensions: 20852
Number of successful extensions: 256
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 28
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 495302128
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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