Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP05_F_I20
(884 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g06360.1 68418.m00712 ribosomal protein S8e family protein co... 261 5e-70
At5g41320.1 68418.m05022 expressed protein 33 0.33
At5g25410.1 68418.m03014 expressed protein contains Pfam profil... 31 0.77
At4g11170.1 68417.m01809 disease resistance protein (TIR-NBS-LRR... 31 0.77
At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N domain-... 31 0.77
At5g23780.1 68418.m02792 agenet domain-containing protein contai... 31 1.0
At3g20550.1 68416.m02601 forkhead-associated domain-containing p... 31 1.3
At4g20160.1 68417.m02949 expressed protein ; expression supporte... 30 2.3
At2g21090.1 68415.m02503 pentatricopeptide (PPR) repeat-containi... 30 2.3
At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar... 30 2.3
At3g56720.1 68416.m06309 expressed protein 29 4.1
At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains... 29 5.4
At1g76890.2 68414.m08948 trihelix DNA-binding protein / GT-2 fac... 29 5.4
At1g76890.1 68414.m08947 trihelix DNA-binding protein / GT-2 fac... 29 5.4
At5g53800.1 68418.m06685 expressed protein 28 7.2
At5g48690.1 68418.m06025 hypothetical protein 28 7.2
At5g27860.1 68418.m03342 expressed protein 28 7.2
At3g51070.1 68416.m05592 dehydration-responsive protein-related ... 28 7.2
At2g46810.1 68415.m05841 basic helix-loop-helix (bHLH) family pr... 28 7.2
At2g39260.1 68415.m04821 MIF4G domain-containing protein similar... 28 7.2
At5g41550.1 68418.m05049 disease resistance protein (TIR-NBS-LRR... 28 9.5
At4g35940.1 68417.m05113 expressed protein 28 9.5
At2g40950.1 68415.m05056 bZIP transcription factor family protei... 28 9.5
>At5g06360.1 68418.m00712 ribosomal protein S8e family protein
contains Pfam profile PF01201: Ribosomal protein S8e
Length = 260
Score = 261 bits (639), Expect = 5e-70
Identities = 119/164 (72%), Positives = 137/164 (83%)
Frame = +2
Query: 335 ETQRKVAEGALPVYLLDRDVQSRAKVLSNMIKQKRKEKAGKWDVPIPKVRAQADAEVFKV 514
+ V EGA+P YLLDR+ +RAKVLSN IKQKRKEKAGKW+VP+PKVR A+ E+F+V
Sbjct: 76 KADENVQEGAVPAYLLDREDTTRAKVLSNTIKQKRKEKAGKWEVPLPKVRPVAEDEMFRV 135
Query: 515 LKSGKSKRKAWKRMVTKVTFVGENFTRKPPKFERFIRPMALRFKKGHVTHPELKATFCLP 694
++SGK K K WKRMVTK TFVG FTRKPPK+ERFIRP LRF K HVTHPELK TFCL
Sbjct: 136 IRSGKRKTKQWKRMVTKATFVGPAFTRKPPKYERFIRPSGLRFTKAHVTHPELKCTFCLE 195
Query: 695 IIGVKKNPSSQMYTSLGVITKGTVIEVNISELGLVTQAGKVVWG 826
IIG+KKNP+ MYTSLGV+T+GT+IEVN+SELGLVT AGKVVWG
Sbjct: 196 IIGIKKNPNGPMYTSLGVMTRGTIIEVNVSELGLVTPAGKVVWG 239
Score = 49.2 bits (112), Expect = 4e-06
Identities = 21/24 (87%), Positives = 22/24 (91%)
Frame = +3
Query: 813 KLCGGKYAQVTNNPENDGCINAVL 884
K+ GKYAQVTNNPENDGCINAVL
Sbjct: 235 KVVWGKYAQVTNNPENDGCINAVL 258
Score = 40.3 bits (90), Expect = 0.002
Identities = 22/57 (38%), Positives = 31/57 (54%), Gaps = 3/57 (5%)
Frame = +1
Query: 190 KARAPHKRAEKARKLRGIKAKXFNKERRNEKIQMKKKIKAHER---ERMSNRNTEKG 351
+AR HK + A+K GIK K K+ EK MKK +K HE R ++ N ++G
Sbjct: 28 EAREVHKHSTMAQKSLGIKGKMIAKKNYAEKALMKKTLKMHEESSSRRKADENVQEG 84
>At5g41320.1 68418.m05022 expressed protein
Length = 515
Score = 32.7 bits (71), Expect = 0.33
Identities = 20/67 (29%), Positives = 33/67 (49%), Gaps = 1/67 (1%)
Frame = +1
Query: 124 ARHQKLXGXSGFAFCGAQTESVKARAP-HKRAEKARKLRGIKAKXFNKERRNEKIQMKKK 300
A ++L CGA E+V +R P +KR A + K K+++ E+ +MKKK
Sbjct: 120 ASRERLMDWVKTELCGAPNENVASRPPPNKRGVAAVAAKRPVKKRMTKKKKEEEEKMKKK 179
Query: 301 IKAHERE 321
+ +E
Sbjct: 180 EEEETKE 186
>At5g25410.1 68418.m03014 expressed protein contains Pfam profile
PF03080: Arabidopsis proteins of unknown function
Length = 369
Score = 31.5 bits (68), Expect = 0.77
Identities = 14/33 (42%), Positives = 18/33 (54%), Gaps = 1/33 (3%)
Frame = +1
Query: 271 RNEKIQMKKKIKAHE-RERMSNRNTEKGRRRCP 366
+N KIQMK + HE R + N T K + CP
Sbjct: 60 KNHKIQMKPSVSRHELRNQTDNTKTYKNKMGCP 92
>At4g11170.1 68417.m01809 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1095
Score = 31.5 bits (68), Expect = 0.77
Identities = 15/40 (37%), Positives = 26/40 (65%)
Frame = +2
Query: 407 KVLSNMIKQKRKEKAGKWDVPIPKVRAQADAEVFKVLKSG 526
+VL + ++ K KE+ W+ +P ++++ D EV KVLK G
Sbjct: 386 RVLGSFMRGKGKEE---WEFSLPTLKSRLDGEVEKVLKVG 422
>At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N
domain-containing protein weak similarity to SP|P45974
Ubiquitin carboxyl-terminal hydrolase 5 (EC 3.1.2.15)
{Homo sapiens}; contains Pfam profile PF00627: UBA/TS-N
domain
Length = 413
Score = 31.5 bits (68), Expect = 0.77
Identities = 20/56 (35%), Positives = 31/56 (55%)
Frame = +1
Query: 181 ESVKARAPHKRAEKARKLRGIKAKXFNKERRNEKIQMKKKIKAHERERMSNRNTEK 348
E VK +A R E+ARK + + K +ER E+I++ K++ E +RM N K
Sbjct: 179 EEVKLKAQELR-ERARKKKEEEEKRMEREREKERIRIGKELL--EAKRMEEVNERK 231
>At5g23780.1 68418.m02792 agenet domain-containing protein contains
Pfam PF05641: Agenet domain
Length = 426
Score = 31.1 bits (67), Expect = 1.0
Identities = 22/91 (24%), Positives = 38/91 (41%)
Frame = +1
Query: 52 SLVGLVXSVSCRISNQQCRSHASLARHQKLXGXSGFAFCGAQTESVKARAPHKRAEKARK 231
S VG++ + C + + H + L S FC + +AP R K
Sbjct: 294 SAVGMMLTFPCLLEEVKSLQHDNSI--SSLISLSN-NFCELEKHGFNVKAPQSRISKLLS 350
Query: 232 LRGIKAKXFNKERRNEKIQMKKKIKAHERER 324
LRG ++ ++ + EK+ +K+ E ER
Sbjct: 351 LRGKQSMKMDELKGAEKVTAEKESIKIENER 381
>At3g20550.1 68416.m02601 forkhead-associated domain-containing
protein / FHA domain-containing protein weak similarity
to SP|Q28147 Nuclear inhibitor of protein phosphatase-1
(NIPP-1) (Protein phosphatase 1, regulatory inhibitor
subunit 8) {Bos taurus}; contains Pfam profile PF00498:
FHA domain
Length = 314
Score = 30.7 bits (66), Expect = 1.3
Identities = 15/55 (27%), Positives = 33/55 (60%)
Frame = +1
Query: 193 ARAPHKRAEKARKLRGIKAKXFNKERRNEKIQMKKKIKAHERERMSNRNTEKGRR 357
+R+P R ++ R+ RG K ++ER ++ + +K+ + ER+R R+ ++ R+
Sbjct: 5 SRSPSPRTKRLRRARGEKEIGRSREREDDGREREKR-NSRERDRDIGRDRDRERK 58
>At4g20160.1 68417.m02949 expressed protein ; expression supported
by MPSS
Length = 1188
Score = 29.9 bits (64), Expect = 2.3
Identities = 18/62 (29%), Positives = 28/62 (45%), Gaps = 1/62 (1%)
Frame = +1
Query: 181 ESVKARAPHKRAEKARKLRGIKAKXFNKERRNEKIQMKKKIKAHERERMSNRNT-EKGRR 357
E K R + E+ LR +AK + R E Q +K + RE+ RN+ + G +
Sbjct: 762 ERDKERNSSQNGEETLSLRNSEAKSTKEIERQEVTQEEKSVSHGSREKDKERNSLQYGEK 821
Query: 358 RC 363
C
Sbjct: 822 MC 823
>At2g21090.1 68415.m02503 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 597
Score = 29.9 bits (64), Expect = 2.3
Identities = 15/38 (39%), Positives = 24/38 (63%), Gaps = 1/38 (2%)
Frame = +1
Query: 199 APHKRAEKARKLRGI-KAKXFNKERRNEKIQMKKKIKA 309
A H + E KLRG+ K + NKE+ I+++KK++A
Sbjct: 526 ADHGKWELVEKLRGVMKKRRVNKEKAVSWIEIEKKVEA 563
>At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar to
RNA helicase [Rattus norvegicus] GI:897915; contains
Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
Helicase conserved C-terminal domain
Length = 1166
Score = 29.9 bits (64), Expect = 2.3
Identities = 15/58 (25%), Positives = 31/58 (53%)
Frame = +1
Query: 187 VKARAPHKRAEKARKLRGIKAKXFNKERRNEKIQMKKKIKAHERERMSNRNTEKGRRR 360
V+ A K++ + R + K E+R EK + KK++K+ + E +R+ ++ R +
Sbjct: 16 VEEEADLKKSRRDRDRSNERKKDKGSEKRREKDRRKKRVKSSDSEDDYDRDDDEEREK 73
Score = 28.7 bits (61), Expect = 5.4
Identities = 17/61 (27%), Positives = 31/61 (50%), Gaps = 2/61 (3%)
Frame = +1
Query: 181 ESVKARAPHKRAEKARKLRGIKAKXFNK--ERRNEKIQMKKKIKAHERERMSNRNTEKGR 354
E + R ++ ++ R+ R K + K ERR + + ++K + ERER + E+ R
Sbjct: 125 EHERDRGKDRKRDREREERKDKEREREKDRERREREREEREKERVKERERREREDGERDR 184
Query: 355 R 357
R
Sbjct: 185 R 185
>At3g56720.1 68416.m06309 expressed protein
Length = 386
Score = 29.1 bits (62), Expect = 4.1
Identities = 12/31 (38%), Positives = 21/31 (67%)
Frame = +1
Query: 208 KRAEKARKLRGIKAKXFNKERRNEKIQMKKK 300
+R EK KL G+KAK N+E+ +++ +K+
Sbjct: 329 ERQEKFNKLMGVKAKVVNQEQNLGEVEAEKQ 359
>At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains
ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017
Length = 1340
Score = 28.7 bits (61), Expect = 5.4
Identities = 16/51 (31%), Positives = 27/51 (52%)
Frame = +1
Query: 208 KRAEKARKLRGIKAKXFNKERRNEKIQMKKKIKAHERERMSNRNTEKGRRR 360
++ ++A LR + +ERR E + + K + ERER +R E+ R R
Sbjct: 342 RKGDRASYLREFDLR--EEERRREDQRARDKEREREREREHDRERERQRER 390
>At1g76890.2 68414.m08948 trihelix DNA-binding protein / GT-2 factor
(GT2) identical to GT2 factor [Arabidopsis thaliana]
GI:416490, GI:2664202 (DNA binding factor GT-2 from
Arabidopsis)
Length = 575
Score = 28.7 bits (61), Expect = 5.4
Identities = 17/55 (30%), Positives = 28/55 (50%)
Frame = +2
Query: 485 AQADAEVFKVLKSGKSKRKAWKRMVTKVTFVGENFTRKPPKFERFIRPMALRFKK 649
A + E +KS + KRK WK + TK+T E ++ +RF+ + R K+
Sbjct: 241 ASDEEEDHHQVKSSRKKRKYWKGLFTKLT--KELMEKQEKMQKRFLETLEYREKE 293
>At1g76890.1 68414.m08947 trihelix DNA-binding protein / GT-2 factor
(GT2) identical to GT2 factor [Arabidopsis thaliana]
GI:416490, GI:2664202 (DNA binding factor GT-2 from
Arabidopsis)
Length = 498
Score = 28.7 bits (61), Expect = 5.4
Identities = 17/55 (30%), Positives = 28/55 (50%)
Frame = +2
Query: 485 AQADAEVFKVLKSGKSKRKAWKRMVTKVTFVGENFTRKPPKFERFIRPMALRFKK 649
A + E +KS + KRK WK + TK+T E ++ +RF+ + R K+
Sbjct: 164 ASDEEEDHHQVKSSRKKRKYWKGLFTKLT--KELMEKQEKMQKRFLETLEYREKE 216
>At5g53800.1 68418.m06685 expressed protein
Length = 351
Score = 28.3 bits (60), Expect = 7.2
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +3
Query: 282 DSDEKENQSARKRKNVKQKHRERSPK 359
DS+E E++ R+R+ K+K RE K
Sbjct: 120 DSEESESEDERRRRKRKRKEREEEEK 145
>At5g48690.1 68418.m06025 hypothetical protein
Length = 301
Score = 28.3 bits (60), Expect = 7.2
Identities = 19/56 (33%), Positives = 32/56 (57%)
Frame = +1
Query: 181 ESVKARAPHKRAEKARKLRGIKAKXFNKERRNEKIQMKKKIKAHERERMSNRNTEK 348
E+ ARA + +E+ARKLR + +ER E+I+ K++ E +R++ N K
Sbjct: 59 ETSHARAK-ELSEQARKLREEQETKREREREKERIRAGKELM--ETKRIAEENERK 111
>At5g27860.1 68418.m03342 expressed protein
Length = 177
Score = 28.3 bits (60), Expect = 7.2
Identities = 11/36 (30%), Positives = 21/36 (58%)
Frame = +1
Query: 244 KAKXFNKERRNEKIQMKKKIKAHERERMSNRNTEKG 351
K++ K + +K + K++ K+H +R NR +KG
Sbjct: 80 KSRRHKKHEKPKKAKDKERSKSHRHKRHKNRERKKG 115
>At3g51070.1 68416.m05592 dehydration-responsive protein-related
similar to early-responsive to dehydration stress ERD3
protein [Arabidopsis thaliana] GI:15320410; contains
Pfam profile PF03141: Putative methyltransferase
Length = 895
Score = 28.3 bits (60), Expect = 7.2
Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 4/64 (6%)
Frame = +1
Query: 172 AQTESVKARAPHKRAEKARKLRGIKAKXFNKERR----NEKIQMKKKIKAHERERMSNRN 339
++T S K + + K+ K K KE + +E+ QMKK +K E+E+ R+
Sbjct: 113 SETTSSKTQTQETQQNNDDKISEEKEKDNGKENQTVQESEEGQMKKVVKEFEKEQKQQRD 172
Query: 340 TEKG 351
+ G
Sbjct: 173 EDAG 176
>At2g46810.1 68415.m05841 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 371
Score = 28.3 bits (60), Expect = 7.2
Identities = 13/50 (26%), Positives = 27/50 (54%)
Frame = +1
Query: 211 RAEKARKLRGIKAKXFNKERRNEKIQMKKKIKAHERERMSNRNTEKGRRR 360
R+ + RK + + ++R+ + + K I+ E +RM++ E+ RRR
Sbjct: 155 RSRRKRKNNNLASLMTREKRKRRRTKPTKNIEEIESQRMTHIAVERNRRR 204
>At2g39260.1 68415.m04821 MIF4G domain-containing protein similar to
hUPF2 [Homo sapiens] GI:12232320; contains Pfam profile
PF02854: MIF4G domain
Length = 1186
Score = 28.3 bits (60), Expect = 7.2
Identities = 20/71 (28%), Positives = 36/71 (50%), Gaps = 3/71 (4%)
Frame = +1
Query: 172 AQTESVKARAPHKRAEKARKLRGIKAKXFNKERRNEKIQMKKKIKAHERER---MSNRNT 342
A + S+ R+ ++ ++ ++ KAK KE+ EK KK HE+E+ + N
Sbjct: 410 ADSASMDDRSNAEQPKEKEEVEKEKAKDTKKEKGKEKDSEKK--MEHEKEKGKSLDVANF 467
Query: 343 EKGRRRCPACL 375
E+ +R P C+
Sbjct: 468 ERLLQRLPGCV 478
>At5g41550.1 68418.m05049 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1085
Score = 27.9 bits (59), Expect = 9.5
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = +2
Query: 437 RKEKAGKWDVPIPKVRAQADAEVFKVLKSG 526
R E +W++ +P++ A D ++ +LK G
Sbjct: 396 RGESKHEWELQLPRIEASLDGKIESILKVG 425
>At4g35940.1 68417.m05113 expressed protein
Length = 451
Score = 27.9 bits (59), Expect = 9.5
Identities = 20/74 (27%), Positives = 34/74 (45%)
Frame = +1
Query: 139 LXGXSGFAFCGAQTESVKARAPHKRAEKARKLRGIKAKXFNKERRNEKIQMKKKIKAHER 318
L G A + + V+ +A ++ K R+ K K KER+ +K + +KK K E
Sbjct: 14 LNGIRDEAVIVSSIKGVEEKAKKEQRRKDRR-SDKKDKKDKKERKEKKEKKEKKRKEREG 72
Query: 319 ERMSNRNTEKGRRR 360
+ + + RRR
Sbjct: 73 KEVGSEKRSHKRRR 86
>At2g40950.1 68415.m05056 bZIP transcription factor family protein
similar to AtbZIP transcription factor GI:17065880 from
[Arabidopsis thaliana]; contains Pfam profile: bZIP
transcription factor PF00170
Length = 721
Score = 27.9 bits (59), Expect = 9.5
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +2
Query: 464 VPIPKVRAQADAEVFKVLKSGKSKRKAWKRMVTKVTFVG 580
+PIP+++ Q K KS K +A + V ++F+G
Sbjct: 334 IPIPRLKPQNTLGTSKAKKSESKKSEAKTKKVASISFLG 372
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,793,440
Number of Sequences: 28952
Number of extensions: 290633
Number of successful extensions: 1085
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 980
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1062
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2071520424
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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